1mow - Revision history

OCA at 07:46, 14 February 2024

2024-02-14T07:46:45Z

←Older revision		Revision as of 07:46, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35582_[[desulfurococcus_mobilis~~]~~] Atcc 35582~~ [~~[desulfurococcus mobilis]]~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Desulfurococcus_mucosus Desulfurococcus mucosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [https://pdbe.org/1mow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [https://www.ebi.ac.uk/pdbsum/1mow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [https://pdbe.org/1mow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [https://www.ebi.ac.uk/pdbsum/1mow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO]] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.	+	[https://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mow ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mow ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.
-
-	Design, activity, and structure of a highly specific artificial endonuclease.,Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL Mol Cell. 2002 Oct;10(4):895-905. PMID:12419232<ref>PMID:12419232</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlamydomonas reinhardtii]]
		+	[[Category: Desulfurococcus mucosus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker, D]]	+	[[Category: Baker D]]
-	[[Category: Chadsey~~, M S~~]]	+	[[Category: Chadsey MS]]
-	[[Category: Chevalier~~, B S~~]]	+	[[Category: Chevalier BS]]
-	[[Category: Kortemme, T]]	+	[[Category: Kortemme T]]
-	[[Category: Monnat~~, R J~~]]	+	[[Category: Monnat Jr RJ]]
-	[[Category: Stoddard~~, B L]]~~	+	[[Category: Stoddard BL]]
-	~~[[Category: Design]]~~	+
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Engineering]]~~	+
-	~~[[Category: Homing]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Laglidadg~~]]	+

OCA at 09:53, 26 May 2021

2021-05-26T09:53:13Z

←Older revision		Revision as of 09:53, 26 May 2021
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	<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MOW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [~~http~~://pdbe.org/1mow PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mow PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [https://pdbe.org/1mow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [https://www.ebi.ac.uk/pdbsum/1mow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO]] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.	+	[[https://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO]] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:13, 21 August 2019

2019-08-21T07:13:40Z

←Older revision		Revision as of 07:13, 21 August 2019
Line 1:		Line 1:

	==E-DreI==		==E-DreI==
-	<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1mow' size='340' side='right'caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1mow_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1mow_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Baker, D]]		[[Category: Baker, D]]
	[[Category: Chadsey, M S]]		[[Category: Chadsey, M S]]

OCA at 02:04, 16 November 2017

2017-11-16T02:04:20Z

←Older revision		Revision as of 02:04, 16 November 2017
Line 1:		Line 1:
		+
	==E-DreI==		==E-DreI==
	<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_35582~~ Atcc 35582]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35582_[[desulfurococcus_mobilis]] Atcc 35582 [[desulfurococcus mobilis]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://pdbe.org/1mow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://pdbe.org/1mow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mow ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35582]]
	[[Category: Baker, D]]		[[Category: Baker, D]]
	[[Category: Chadsey, M S]]		[[Category: Chadsey, M S]]

OCA at 04:24, 10 February 2016

2016-02-10T04:24:17Z

←Older revision		Revision as of 04:24, 10 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mow ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:21, 11 September 2015

2015-09-11T05:21:08Z

←Older revision		Revision as of 05:21, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35582 Atcc 35582]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://pdbe.org/1mow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mow" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlamydomonas reinhardtii~~]]	+	[[Category: Atcc 35582]]
	[[Category: Baker, D]]		[[Category: Baker, D]]
	[[Category: Chadsey, M S]]		[[Category: Chadsey, M S]]

OCA at 11:46, 25 December 2014

2014-12-25T11:46:28Z

←Older revision		Revision as of 11:46, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DMO1_DESMO DMO1_DESMO]] Endonuclease involved in intron homing. Recognizes a recognizes up to 20 bp of DNA in the 23S rRNA gene intron. It has a slow turnover rate and cuts the coding strand with a slight preference over the non-coding strand.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
-	[[Category: Baker, D.]]	+	[[Category: Baker, D]]
-	[[Category: Chadsey, M S.]]	+	[[Category: Chadsey, M S]]
-	[[Category: Chevalier, B S.]]	+	[[Category: Chevalier, B S]]
-	[[Category: Kortemme, T.]]	+	[[Category: Kortemme, T]]
-	[[Category: Monnat, R J.]]	+	[[Category: Monnat, R J]]
-	[[Category: Stoddard, B L.]]	+	[[Category: Stoddard, B L]]
	[[Category: Design]]		[[Category: Design]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]

OCA at 13:43, 28 September 2014

2014-09-28T13:43:05Z

←Older revision		Revision as of 13:43, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1mow.~~png\|left~~\|200px]]	+	==E-DreI==
		+	<StructureSection load='1mow' size='340' side='right' caption='[[1mow]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MOW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1mow_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.

-	~~{{STRUCTURE_1mow\| PDB=1mow \| SCENE= }}~~	+	Design, activity, and structure of a highly specific artificial endonuclease.,Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL Mol Cell. 2002 Oct;10(4):895-905. PMID:12419232<ref>PMID:12419232</ref>

-	~~===E-DreI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12419232}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1mow]] is~~ a ~~12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA].~~	+

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012419232<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Baker, D.]]		[[Category: Baker, D.]]

OCA at 16:19, 20 October 2012

2012-10-20T16:19:20Z

←Older revision		Revision as of 16:19, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1mow]] is a 12 chain structure ~~of [[Endonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/~~Desulfurococcus_mobilis,_chlamydomonas_reinhardtii Desulfurococcus mobilis, chlamydomonas~~ reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA].	+	[[1mow]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOW OCA].

	==See Also==		==See Also==
Line 15:		Line 15:
	==Reference==		==Reference==
	<ref group="xtra">PMID:012419232</ref><references group="xtra"/>		<ref group="xtra">PMID:012419232</ref><references group="xtra"/>
-	[[Category: ~~Desulfurococcus mobilis, chlamydomonas~~ reinhardtii]]	+	[[Category: Chlamydomonas reinhardtii]]
	[[Category: Baker, D.]]		[[Category: Baker, D.]]
	[[Category: Chadsey, M S.]]		[[Category: Chadsey, M S.]]

OCA: Protected "1mow" [edit=sysop:move=sysop]

2012-07-26T21:18:03Z

Protected "1mow" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:18, 26 July 2012