1msc - Revision history

OCA at 07:48, 14 February 2024

2024-02-14T07:48:01Z

←Older revision		Revision as of 07:48, 14 February 2024
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	<StructureSection load='1msc' size='340' side='right'caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1msc' size='340' side='right'caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacteriophage_ms2 Bacteriophage ms2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1MSC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~MS2 COAT GENE (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=12022 Bacteriophage MS2])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [~~http~~://pdbe.org/1msc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1msc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1msc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [https://pdbe.org/1msc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [https://www.ebi.ac.uk/pdbsum/1msc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1msc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~COAT_BPMS2 COAT_BPMS2~~]~~] Forms~~ the ~~phage~~ shell~~; binds~~ to the ~~phage~~ RNA.	+	[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: The coat protein in RNA bacteriophages binds and encapsidates viral RNA, and also acts as translational repressor of viral replicase by binding to an RNA hairpin in the RNA genome. Because of its dual function, the MS2 coat protein is an interesting candidate for structural studies of protein-RNA interactions and protein-protein interactions. In this study, unassembled MS2 coat protein dimers were selected to analyze repressor activity and virus assembly. RESULTS: The crystal structure of a mutant MS2 coat protein that is defective in viral assembly yet retains repressor activity has been determined at 2.0 A resolution. The unassembled dimer is stabilized by interdigitation of alpha-helices, and the formation of a 10-stranded antiparallel beta-sheet across the interface between monomers. The substitution of arginine for tryptophan at residue 82 results in the formation of two new inter-subunit hydrogen bonds that further stabilize the dimer. Residues that influence RNA recognition, identified by molecular genetics, were located across the beta-sheet. Two of these residues (Tyr85 and Asn87) are displaced in the unliganded dimer and are located in the same beta-strand as the Trp-->Arg mutation. CONCLUSIONS: When compared with the structure of the coat protein in the assembled virus, differences in orientation of residues 85 and 87 suggest conformational adjustment on binding RNA in the first step of viral assembly. The substitution at residue 82 may affect virus assembly by imposing conformational restriction on the loop that makes critical inter-subunit contacts in the capsid.
-
-	Crystal structure of the MS2 coat protein dimer: implications for RNA binding and virus assembly.,Ni CZ, Syed R, Kodandapani R, Wickersham J, Peabody DS, Ely KR Structure. 1995 Mar 15;3(3):255-63. PMID:7788292<ref>PMID:7788292</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1msc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage ms2~~]]	+	[[Category: Escherichia phage MS2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ely~~, K R~~]]	+	[[Category: Ely KR]]
-	[[Category: Kodandapani, R]]	+	[[Category: Kodandapani R]]
-	[[Category: Ni, C Z]]	+	[[Category: Ni C-Z]]
-	~~[[Category: Translation repressor]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 08:41, 2 December 2020

2020-12-02T08:41:01Z

←Older revision		Revision as of 08:41, 2 December 2020
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	==CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER==		==CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER==
-	<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1msc' size='340' side='right'caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MSC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Bacteriophage MS2])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Bacteriophage MS2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://pdbe.org/1msc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1msc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://pdbe.org/1msc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1msc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage ms2]]		[[Category: Bacteriophage ms2]]
		+	[[Category: Large Structures]]
	[[Category: Ely, K R]]		[[Category: Ely, K R]]
	[[Category: Kodandapani, R]]		[[Category: Kodandapani, R]]

OCA at 07:57, 31 January 2018

2018-01-31T07:57:52Z

←Older revision		Revision as of 07:57, 31 January 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER==		==CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER==
	<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Bacteriophage MS2])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Bacteriophage MS2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://pdbe.org/1msc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://pdbe.org/1msc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1msc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:25, 8 February 2016

2016-02-08T04:25:52Z

←Older revision		Revision as of 04:25, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:51, 10 September 2015

2015-09-10T07:51:14Z

←Older revision		Revision as of 07:51, 10 September 2015
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	<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacterio_phage_ms2 Enterobacterio phage~~ ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 ~~Enterobacterio phage~~ MS2])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Bacteriophage MS2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://pdbe.org/1msc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1msc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacterio phage~~ ms2]]	+	[[Category: Bacteriophage ms2]]
	[[Category: Ely, K R]]		[[Category: Ely, K R]]
	[[Category: Kodandapani, R]]		[[Category: Kodandapani, R]]

OCA at 20:36, 24 December 2014

2014-12-24T20:36:24Z

←Older revision		Revision as of 20:36, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
-	[[Category: Ely, K R.]]	+	[[Category: Ely, K R]]
-	[[Category: Kodandapani, R.]]	+	[[Category: Kodandapani, R]]
-	[[Category: Ni, C Z.]]	+	[[Category: Ni, C Z]]
	[[Category: Translation repressor]]		[[Category: Translation repressor]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 16:06, 28 September 2014

2014-09-28T16:06:14Z

←Older revision		Revision as of 16:06, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1msc.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER==
		+	<StructureSection load='1msc' size='340' side='right' caption='[[1msc]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MSC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MS2 COAT GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1msc RCSB], [http://www.ebi.ac.uk/pdbsum/1msc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The coat protein in RNA bacteriophages binds and encapsidates viral RNA, and also acts as translational repressor of viral replicase by binding to an RNA hairpin in the RNA genome. Because of its dual function, the MS2 coat protein is an interesting candidate for structural studies of protein-RNA interactions and protein-protein interactions. In this study, unassembled MS2 coat protein dimers were selected to analyze repressor activity and virus assembly. RESULTS: The crystal structure of a mutant MS2 coat protein that is defective in viral assembly yet retains repressor activity has been determined at 2.0 A resolution. The unassembled dimer is stabilized by interdigitation of alpha-helices, and the formation of a 10-stranded antiparallel beta-sheet across the interface between monomers. The substitution of arginine for tryptophan at residue 82 results in the formation of two new inter-subunit hydrogen bonds that further stabilize the dimer. Residues that influence RNA recognition, identified by molecular genetics, were located across the beta-sheet. Two of these residues (Tyr85 and Asn87) are displaced in the unliganded dimer and are located in the same beta-strand as the Trp-->Arg mutation. CONCLUSIONS: When compared with the structure of the coat protein in the assembled virus, differences in orientation of residues 85 and 87 suggest conformational adjustment on binding RNA in the first step of viral assembly. The substitution at residue 82 may affect virus assembly by imposing conformational restriction on the loop that makes critical inter-subunit contacts in the capsid.

-	~~{{STRUCTURE_1msc\| PDB=1msc \| SCENE= }}~~	+	Crystal structure of the MS2 coat protein dimer: implications for RNA binding and virus assembly.,Ni CZ, Syed R, Kodandapani R, Wickersham J, Peabody DS, Ely KR Structure. 1995 Mar 15;3(3):255-63. PMID:7788292<ref>PMID:7788292</ref>

-	~~===CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7788292}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1msc]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007788292<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
	[[Category: Ely, K R.]]		[[Category: Ely, K R.]]

OCA: Protected "1msc" [edit=sysop:move=sysop]

2012-12-05T21:04:01Z

Protected "1msc" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:04, 5 December 2012

OCA at 21:03, 5 December 2012

2012-12-05T21:03:58Z

←Older revision		Revision as of 21:03, 5 December 2012
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-	{{Seed}}
	[[Image:1msc.png\|left\|200px]]		[[Image:1msc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1msc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1msc\| PDB=1msc \| SCENE= }}		{{STRUCTURE_1msc\| PDB=1msc \| SCENE= }}

	===CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER===		===CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_7788292}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 7788292 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_7788292}}		{{ABSTRACT_PUBMED_7788292}}

	==About this Structure==		==About this Structure==
-	~~1MSC~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA].	+	[[1msc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~7788292~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007788292</ref><references group="xtra"/>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
	[[Category: Ely, K R.]]		[[Category: Ely, K R.]]
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	[[Category: Ni, C Z.]]		[[Category: Ni, C Z.]]
	[[Category: Translation repressor]]		[[Category: Translation repressor]]
-		+	[[Category: Viral protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 18:47:49 2009''~~	+

OCA at 16:47, 16 February 2009

2009-02-16T16:47:51Z

←Older revision		Revision as of 16:47, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1MSC is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA].	+	1MSC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSC OCA].

	==Reference==		==Reference==
-	~~Crystal structure of the MS2 coat protein dimer: implications for RNA binding and virus assembly., Ni CZ, Syed R, Kodandapani R, Wickersham J, Peabody DS, Ely KR, Structure. 1995 Mar 15;3(3):255-63.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/7788292 7788292]~~	+	<ref group="xtra">PMID:7788292</ref><references group="xtra"/>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
-	[[Category: Single protein]]
	[[Category: Ely, K R.]]		[[Category: Ely, K R.]]
	[[Category: Kodandapani, R.]]		[[Category: Kodandapani, R.]]
Line 31:		Line 30:
	[[Category: Translation repressor]]		[[Category: Translation repressor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 23~~:05:~~08 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:47:49 2009''