1mul - Revision history

OCA at 13:27, 13 March 2024

2024-03-13T13:27:04Z

←Older revision		Revision as of 13:27, 13 March 2024
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	<StructureSection load='1mul' size='340' side='right'caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1mul' size='340' side='right'caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1MUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [~~http~~://pdbe.org/1mul PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mul PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mul ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [https://pdbe.org/1mul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [https://www.ebi.ac.uk/pdbsum/1mul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mul ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DBHA_ECOLI DBHA_ECOLI]] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.	+	[https://www.uniprot.org/uniprot/DBHA_ECOLI DBHA_ECOLI] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mul ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mul ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Escherichia coli histone-like HU protein pool is composed of three dimeric forms: two homodimers, EcHUalpha(2) and EcHUbeta(2), and a heterodimer, EcHUalphabeta. The relative abundance of these dimeric forms varies during cell growth and in response to environmental changes, suggesting that each dimer plays different physiological roles. Here, differential scanning calorimetry and circular dichroism (CD) were used to study the thermal stability of the three E.coli HU dimers and show that each of them has its own thermodynamic signature. Unlike the other HU proteins studied so far, which melt through a single step (N(2)<-->2D), this present thermodynamic study shows that the three E.coli dimers melt according to a two-step mechanism (N(2)<-->I(2)<-->2D). The native dimer, N(2), melts partially into a dimeric intermediate, I(2), which in turn yields the unfolded monomers, D. In addition, the crystal structure of the EcHUalpha(2) dimer has been solved. Comparative thermodynamic and structural analysis between EcHUalpha(2) and the HU homodimer from Bacillus stearothermophilus suggests that the E.coli dimer is constituted by two subdomains of different energetic properties. The CD study indicates that the intermediate, I(2), corresponds to an HU dimer having partly lost its alpha-helices. The partially unfolded dimer I(2) is unable to complex with high-affinity, single-stranded break-containing DNA. These structural, thermodynamic and functional results suggest that the N(2)<-->I(2) equilibrium plays a central role in the physiology of E.coli HU. The I(2) molecular species seems to be the EcHUbeta(2) preferential conformation, possibly related to its role in the E.coli cold-shock adaptation. Besides, I(2) might be required in E.coli for the HU chain exchange, which allows the heterodimer formation from homodimers.
-
-	Evidence of a thermal unfolding dimeric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure.,Ramstein J, Hervouet N, Coste F, Zelwer C, Oberto J, Castaing B J Mol Biol. 2003 Aug 1;331(1):101-21. PMID:12875839<ref>PMID:12875839</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mul" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Castaing, B]]	+	[[Category: Castaing B]]
-	[[Category: Coste, F]]	+	[[Category: Coste F]]
-	[[Category: Hervouet, N]]	+	[[Category: Hervouet N]]
-	[[Category: Oberto, J]]	+	[[Category: Oberto J]]
-	[[Category: Ramstein, J]]	+	[[Category: Ramstein J]]
-	[[Category: Zelwer, C]]	+	[[Category: Zelwer C]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Histone-like~~]]	+

OCA at 08:41, 2 December 2020

2020-12-02T08:41:38Z

←Older revision		Revision as of 08:41, 2 December 2020
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	==Crystal structure of the E. coli HU alpha2 protein==		==Crystal structure of the E. coli HU alpha2 protein==
-	<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1mul' size='340' side='right'caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://pdbe.org/1mul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mul ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://pdbe.org/1mul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mul ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1mul_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1mul_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Castaing, B]]		[[Category: Castaing, B]]
	[[Category: Coste, F]]		[[Category: Coste, F]]

OCA at 10:21, 25 October 2017

2017-10-25T10:21:13Z

←Older revision		Revision as of 10:21, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the E. coli HU alpha2 protein==		==Crystal structure of the E. coli HU alpha2 protein==
	<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://pdbe.org/1mul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://pdbe.org/1mul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mul ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mul ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:05, 12 September 2015

2015-09-12T01:05:48Z

←Older revision		Revision as of 01:05, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://pdbe.org/1mul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mul" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Castaing, B]]		[[Category: Castaing, B]]
	[[Category: Coste, F]]		[[Category: Coste, F]]

OCA at 21:48, 25 December 2014

2014-12-25T21:48:05Z

←Older revision		Revision as of 21:48, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DBHA_ECOLI DBHA_ECOLI]] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Castaing, B.]]	+	[[Category: Castaing, B]]
-	[[Category: Coste, F.]]	+	[[Category: Coste, F]]
-	[[Category: Hervouet, N.]]	+	[[Category: Hervouet, N]]
-	[[Category: Oberto, J.]]	+	[[Category: Oberto, J]]
-	[[Category: Ramstein, J.]]	+	[[Category: Ramstein, J]]
-	[[Category: Zelwer, C.]]	+	[[Category: Zelwer, C]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Histone-like]]		[[Category: Histone-like]]

OCA at 16:17, 28 September 2014

2014-09-28T16:17:54Z

←Older revision		Revision as of 16:17, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1mul.~~png\|left~~\|200px]]	+	==Crystal structure of the E. coli HU alpha2 protein==
		+	<StructureSection load='1mul' size='340' side='right' caption='[[1mul]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MUL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HUPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mul OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mul RCSB], [http://www.ebi.ac.uk/pdbsum/1mul PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1mul_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Escherichia coli histone-like HU protein pool is composed of three dimeric forms: two homodimers, EcHUalpha(2) and EcHUbeta(2), and a heterodimer, EcHUalphabeta. The relative abundance of these dimeric forms varies during cell growth and in response to environmental changes, suggesting that each dimer plays different physiological roles. Here, differential scanning calorimetry and circular dichroism (CD) were used to study the thermal stability of the three E.coli HU dimers and show that each of them has its own thermodynamic signature. Unlike the other HU proteins studied so far, which melt through a single step (N(2)<-->2D), this present thermodynamic study shows that the three E.coli dimers melt according to a two-step mechanism (N(2)<-->I(2)<-->2D). The native dimer, N(2), melts partially into a dimeric intermediate, I(2), which in turn yields the unfolded monomers, D. In addition, the crystal structure of the EcHUalpha(2) dimer has been solved. Comparative thermodynamic and structural analysis between EcHUalpha(2) and the HU homodimer from Bacillus stearothermophilus suggests that the E.coli dimer is constituted by two subdomains of different energetic properties. The CD study indicates that the intermediate, I(2), corresponds to an HU dimer having partly lost its alpha-helices. The partially unfolded dimer I(2) is unable to complex with high-affinity, single-stranded break-containing DNA. These structural, thermodynamic and functional results suggest that the N(2)<-->I(2) equilibrium plays a central role in the physiology of E.coli HU. The I(2) molecular species seems to be the EcHUbeta(2) preferential conformation, possibly related to its role in the E.coli cold-shock adaptation. Besides, I(2) might be required in E.coli for the HU chain exchange, which allows the heterodimer formation from homodimers.

-	~~{{STRUCTURE_1mul\| PDB=1mul \| SCENE= }}~~	+	Evidence of a thermal unfolding dimeric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure.,Ramstein J, Hervouet N, Coste F, Zelwer C, Oberto J, Castaing B J Mol Biol. 2003 Aug 1;331(1):101-21. PMID:12875839<ref>PMID:12875839</ref>

-	~~===Crystal structure~~ of the E. ~~coli HU alpha2 protein===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12875839}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1mul]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012875839<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Castaing, B.]]		[[Category: Castaing, B.]]

OCA: Protected "1mul" [edit=sysop:move=sysop]

2012-12-05T21:24:14Z

Protected "1mul" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:24, 5 December 2012

OCA at 21:24, 5 December 2012

2012-12-05T21:24:12Z

←Older revision		Revision as of 21:24, 5 December 2012
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-	{{Seed}}
	[[Image:1mul.png\|left\|200px]]		[[Image:1mul.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1mul", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1mul\| PDB=1mul \| SCENE= }}		{{STRUCTURE_1mul\| PDB=1mul \| SCENE= }}

	===Crystal structure of the E. coli HU alpha2 protein===		===Crystal structure of the E. coli HU alpha2 protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12875839}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12875839 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12875839}}		{{ABSTRACT_PUBMED_12875839}}

	==About this Structure==		==About this Structure==
-	~~1MUL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA].	+	[[1mul]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12875839~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012875839</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Castaing, B.]]		[[Category: Castaing, B.]]
Line 31:		Line 19:
	[[Category: Ramstein, J.]]		[[Category: Ramstein, J.]]
	[[Category: Zelwer, C.]]		[[Category: Zelwer, C.]]
		+	[[Category: Dna binding protein]]
	[[Category: Histone-like]]		[[Category: Histone-like]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:52:56 2009''

OCA at 06:52, 18 February 2009

2009-02-18T06:52:57Z

←Older revision		Revision as of 06:52, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1MUL is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA].	+	1MUL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUL OCA].

	==Reference==		==Reference==
-	Evidence of a thermal unfolding dimeric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure., Ramstein J, Hervouet N, Coste F, Zelwer C, Oberto J, Castaing B, J Mol Biol. 2003 Aug 1;331(1):101-21. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12875839 12875839]~~	+	<ref group="xtra">PMID:12875839</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Castaing, B.]]		[[Category: Castaing, B.]]
	[[Category: Coste, F.]]		[[Category: Coste, F.]]
Line 34:		Line 33:
	[[Category: Histone-like]]		[[Category: Histone-like]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:04:~~55 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:52:56 2009''

OCA at 12:04, 29 July 2008

2008-07-29T12:04:58Z

←Older revision		Revision as of 12:04, 29 July 2008
Line 1:		Line 1:
-	[[Image:1mul.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1mul.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1mul\| PDB=1mul \| SCENE= }}		{{STRUCTURE_1mul\| PDB=1mul \| SCENE= }}

-	~~'''~~Crystal structure of the E. coli HU alpha2 protein~~'''~~	+	===Crystal structure of the E. coli HU alpha2 protein===


-	~~==Overview==~~	+	<!--
-	The ~~Escherichia coli histone-like HU protein pool is composed of three dimeric forms: two homodimers~~, ~~EcHUalpha(2) and EcHUbeta(2)~~, ~~and a heterodimer, EcHUalphabeta. The relative abundance of these dimeric forms varies during cell growth and in response~~ to ~~environmental changes, suggesting that each dimer plays different physiological roles. Here, differential scanning calorimetry and circular dichroism (CD) were used to study~~ the ~~thermal stability of the three E.coli HU dimers and show that each of them has its own thermodynamic signature. Unlike the other HU proteins studied so far, which melt through a single step~~ (~~N(2)<-->2D), this present thermodynamic study shows that the three E~~.~~coli dimers melt according to a two-step mechanism (N(2)<-->I(2)<-->2D)~~. ~~The native dimer, N(2~~), melts partially into a dimeric intermediate, I(2), which in turn yields the unfolded monomers, D. In addition, the crystal structure of the EcHUalpha(2) dimer has been solved. Comparative thermodynamic and structural analysis between EcHUalpha(2) and the HU homodimer from Bacillus stearothermophilus suggests that the E.coli dimer is ~~constituted by two subdomains of different energetic properties. The CD study indicates that~~ the ~~intermediate, I(2), corresponds to an HU dimer having partly lost its alpha-helices~~. ~~The partially unfolded dimer I(2) is unable to complex with high~~-~~affinity, single~~-stranded break-containing DNA. These structural, thermodynamic and functional results suggest that the N(2)<-->I(2) equilibrium plays a central role in the physiology of E.coli HU. The I(2) molecular species seems to be the EcHUbeta(2) preferential conformation, possibly related to its role in the E.coli cold-shock adaptation. Besides, I(2) might be required in E.coli for the HU chain exchange, which allows the heterodimer formation from homodimers.	+	The line below this paragraph, {{ABSTRACT_PUBMED_12875839}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12875839 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12875839}}

	==About this Structure==		==About this Structure==
Line 29:		Line 33:
	[[Category: Zelwer, C.]]		[[Category: Zelwer, C.]]
	[[Category: Histone-like]]		[[Category: Histone-like]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 01~~:44:21 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:04:55 2008''