1n5v - Revision history

OCA at 07:53, 14 February 2024

2024-02-14T07:53:14Z

←Older revision		Revision as of 07:53, 14 February 2024
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	<StructureSection load='1n5v' size='340' side='right'caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>		<StructureSection load='1n5v' size='340' side='right'caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strco Strco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1N5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N5V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NOM:7~~-~~HYDROXY-5-METHYL-3~~,~~3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA~~[A]~~ANTHRACENE-~~2~~,6,11-TRIONE'>NOM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.24Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;'>[[1lq9\|1lq9]]~~, ~~[[1n5q\|1n5q]]~~, [~~[1n5s\|1n5s]~~], ~~[[1n5t\|1n5t]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [https://pdbe.org/1n5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [https://www.ebi.ac.uk/pdbsum/1n5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5v ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [~~http~~://pdbe.org/1n5v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1n5v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5v ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q53908_STRCH Q53908_STRCH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5v ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	ActVA-Orf6 monooxygenase from Streptomyces coelicolor that catalyses the oxidation of an aromatic intermediate of the actinorhodin biosynthetic pathway is a member of a class of small monooxygenases that carry out oxygenation without the assistance of any of the prosthetic groups, metal ions or cofactors normally associated with activation of molecular oxygen. The overall structure is a ferredoxin-like fold with a novel dimeric assembly, indicating that the widely represented ferredoxin fold may sustain yet another functionality. The resolution (1.3 A) of the enzyme structure and its complex with substrate and product analogues allows us to visualize the mechanism of binding and activation of the substrate for attack by molecular oxygen, and utilization of two gates for the reaction components including a proton gate and an O(2)/H(2)O gate with a putative protein channel. This is the first crystal structure of an enzyme involved in the tailoring of a type II aromatic polyketide and illustrates some of the enzyme-substrate recognition features that may apply to a range of other enzymes involved in modifying a polyketide core structure.
-
-	The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.,Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B EMBO J. 2003 Jan 15;22(2):205-15. PMID:12514126<ref>PMID:12514126</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]		*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Federici L]]
-	[[Category: Federici, L]]	+	[[Category: Kendrew SG]]
-	[[Category: Kendrew~~, S G~~]]	+	[[Category: Malatesta F]]
-	[[Category: Malatesta, F]]	+	[[Category: Marsh NG]]
-	[[Category: Marsh~~, N G~~]]	+	[[Category: Miele AE]]
-	[[Category: Miele~~, A E~~]]	+	[[Category: Savino C]]
-	[[Category: Savino, C]]	+	[[Category: Schimperna G]]
-	[[Category: Schimperna, G]]	+	[[Category: Sciara G]]
-	[[Category: Sciara, G]]	+	[[Category: Vallone B]]
-	[[Category: Vallone, B]]	+
-	~~[[Category: Actinorhodin]]~~	+
-	~~[[Category: Aromatic polyketide]]~~	+
-	~~[[Category: Dihydrokalafungin]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Nanaomycin d]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Streptomyces coelicolor~~]]	+

OCA at 08:45, 2 December 2020

2020-12-02T08:45:54Z

←Older revision		Revision as of 08:45, 2 December 2020
Line 1:		Line 1:

	==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D==		==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D==
-	<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>	+	<StructureSection load='1n5v' size='340' side='right'caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1N5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1N5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://pdbe.org/1n5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://pdbe.org/1n5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strco]]		[[Category: Strco]]
	[[Category: Federici, L]]		[[Category: Federici, L]]

OCA at 07:39, 7 February 2018

2018-02-07T07:39:06Z

←Older revision		Revision as of 07:39, 7 February 2018
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		+
	==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D==		==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D==
	<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>		<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://pdbe.org/1n5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://pdbe.org/1n5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5v_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5v_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Monooxygenase\|Monooxygenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:22, 10 February 2016

2016-02-10T02:22:13Z

←Older revision		Revision as of 02:22, 10 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:26, 11 September 2015

2015-09-11T04:26:09Z

←Older revision		Revision as of 04:26, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>		<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces coelicolor~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1902 Streptomyces coelicolor~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://pdbe.org/1n5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1n5v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase\|Monooxygenase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces coelicolor~~]]	+	[[Category: Strco]]
	[[Category: Federici, L]]		[[Category: Federici, L]]
	[[Category: Kendrew, S G]]		[[Category: Kendrew, S G]]
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	[[Category: Nanaomycin d]]		[[Category: Nanaomycin d]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
		+	[[Category: Streptomyces coelicolor]]

OCA at 17:12, 5 January 2015

2015-01-05T17:12:37Z

←Older revision		Revision as of 17:12, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N5V FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Federici, L.]]	+	[[Category: Federici, L]]
-	[[Category: Kendrew, S G.]]	+	[[Category: Kendrew, S G]]
-	[[Category: Malatesta, F.]]	+	[[Category: Malatesta, F]]
-	[[Category: Marsh, N G.]]	+	[[Category: Marsh, N G]]
-	[[Category: Miele, A E.]]	+	[[Category: Miele, A E]]
-	[[Category: Savino, C.]]	+	[[Category: Savino, C]]
-	[[Category: Schimperna, G.]]	+	[[Category: Schimperna, G]]
-	[[Category: Sciara, G.]]	+	[[Category: Sciara, G]]
-	[[Category: Vallone, B.]]	+	[[Category: Vallone, B]]
	[[Category: Actinorhodin]]		[[Category: Actinorhodin]]
	[[Category: Aromatic polyketide]]		[[Category: Aromatic polyketide]]
Line 46:		Line 46:
	[[Category: Nanaomycin d]]		[[Category: Nanaomycin d]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Streptomyces coelicolor]]

OCA at 18:47, 28 September 2014

2014-09-28T18:47:21Z

←Older revision		Revision as of 18:47, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1n5v.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D==
		+	<StructureSection load='1n5v' size='340' side='right' caption='[[1n5v]], [[Resolution\|resolution]] 2.24Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N5V FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NOM:7-HYDROXY-5-METHYL-3,3A,5,11B-TETRAHYDRO-1,4-DIOXA-CYCLOPENTA[A]ANTHRACENE-2,6,11-TRIONE'>NOM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq9\|1lq9]], [[1n5q\|1n5q]], [[1n5s\|1n5s]], [[1n5t\|1n5t]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n5v RCSB], [http://www.ebi.ac.uk/pdbsum/1n5v PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5v_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ActVA-Orf6 monooxygenase from Streptomyces coelicolor that catalyses the oxidation of an aromatic intermediate of the actinorhodin biosynthetic pathway is a member of a class of small monooxygenases that carry out oxygenation without the assistance of any of the prosthetic groups, metal ions or cofactors normally associated with activation of molecular oxygen. The overall structure is a ferredoxin-like fold with a novel dimeric assembly, indicating that the widely represented ferredoxin fold may sustain yet another functionality. The resolution (1.3 A) of the enzyme structure and its complex with substrate and product analogues allows us to visualize the mechanism of binding and activation of the substrate for attack by molecular oxygen, and utilization of two gates for the reaction components including a proton gate and an O(2)/H(2)O gate with a putative protein channel. This is the first crystal structure of an enzyme involved in the tailoring of a type II aromatic polyketide and illustrates some of the enzyme-substrate recognition features that may apply to a range of other enzymes involved in modifying a polyketide core structure.

-	~~{{STRUCTURE_1n5v\| PDB=1n5v \| SCENE= }}~~	+	The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.,Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B EMBO J. 2003 Jan 15;22(2):205-15. PMID:12514126<ref>PMID:12514126</ref>

-	~~===Crystal structure of~~ a ~~Monooxygenase from the gene ActVA-Orf6~~ of ~~Streptomyces coelicolor in complex with~~ the ~~ligand Nanaomycin D===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12514126}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1n5v]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012514126<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Federici, L.]]		[[Category: Federici, L.]]

OCA: Protected "1n5v" [edit=sysop:move=sysop]

2012-12-05T21:30:40Z

Protected "1n5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:30, 5 December 2012

OCA at 21:30, 5 December 2012

2012-12-05T21:30:37Z

←Older revision		Revision as of 21:30, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1n5v.png\|left\|200px]]		[[Image:1n5v.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1n5v", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1n5v\| PDB=1n5v \| SCENE= }}		{{STRUCTURE_1n5v\| PDB=1n5v \| SCENE= }}

	===Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D===		===Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Nanaomycin D===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12514126}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12514126 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12514126}}		{{ABSTRACT_PUBMED_12514126}}

	==About this Structure==		==About this Structure==
-	~~1N5V~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA].	+	[[1n5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12514126~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012514126</ref><references group="xtra"/>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Federici, L.]]		[[Category: Federici, L.]]
Line 39:		Line 27:
	[[Category: Monooxygenase]]		[[Category: Monooxygenase]]
	[[Category: Nanaomycin d]]		[[Category: Nanaomycin d]]
		+	[[Category: Oxidoreductase]]
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:39:10 2009''

OCA at 12:39, 17 February 2009

2009-02-17T12:39:11Z

←Older revision		Revision as of 12:39, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1N5V is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA].	+	1N5V is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5V OCA].

	==Reference==		==Reference==
-	The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis., Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B, EMBO J. 2003 Jan 15;22(2):205-15. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12514126 12514126]~~	+	<ref group="xtra">PMID:12514126</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Federici, L.]]		[[Category: Federici, L.]]
Line 42:		Line 41:
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 20~~:07:~~05 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:39:10 2009''