1nbl - Revision history

OCA at 08:47, 10 April 2024

2024-04-10T08:47:41Z

←Older revision		Revision as of 08:47, 10 April 2024
Line 1:		Line 1:

	==NMR Structure of Hellethionin D==		==NMR Structure of Hellethionin D==
-	<StructureSection load='1nbl' size='340' side='right'caption='[[1nbl~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1nbl' size='340' side='right'caption='[[1nbl]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [https://pdbe.org/1nbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [https://www.ebi.ac.uk/pdbsum/1nbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [https://pdbe.org/1nbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [https://www.ebi.ac.uk/pdbsum/1nbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/THND_HELPU THND_HELPU] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nbl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nbl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Thionins are relatively small-sized multiple-cystine peptides that are probably involved in the plant defense against pathogens. As such, these peptides constitute promising candidates for engineered plant resistance in the agricultural industry. More recently, thionins have been proposed as potential immunotoxins in tumor therapy. In the search for pharmacologically active natural products, a new family of thionins was recently discovered in the roots of Helleborus purpurascens that accordingly were termed hellethionins. The structural characterization by NMR of one representative member of this family, i.e., of hellethionin D, clearly reveals that thionins from different sources share a highly conserved overall fold. In fact, the well-defined 3D structure of hellethionin D is very similar to those reported so far for viscotoxins, purothionins, or crambin, although distinct differences could be detected in the C-terminal portion, especially for loop 36-39. These differences may derive from the unusual distribution of charged residues in the C-terminal half of the peptide sequence compared to other thionins and from the uncommon occurrence of four contiguous threonine residues in loop 36-39. As expected, reduction of the disulfide bonds in hellethionin D leads to complete unfolding, but upon oxidative refolding by air oxygen in the presence of glutathione the correct isomer is recovered in high yields, confirming the very robust fold of this class of bioactive cystine peptides.
-
-	Structural characterization of hellethionins from Helleborus purpurascens.,Milbradt AG, Kerek F, Moroder L, Renner C Biochemistry. 2003 Mar 4;42(8):2404-11. PMID:12600207<ref>PMID:12600207</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1nbl" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Helleborus purpurascens]]		[[Category: Helleborus purpurascens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kerek, F]]	+	[[Category: Kerek F]]
-	[[Category: Milbradt~~, A G~~]]	+	[[Category: Milbradt AG]]
-	[[Category: Moroder, L]]	+	[[Category: Moroder L]]
-	[[Category: Renner, C]]	+	[[Category: Renner C]]
-	~~[[Category: Gamma thionins helleborus]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 07:00, 25 August 2021

2021-08-25T07:00:38Z

←Older revision		Revision as of 07:00, 25 August 2021
Line 3:		Line 3:
	<StructureSection load='1nbl' size='340' side='right'caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1nbl' size='340' side='right'caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NBL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [~~http~~://pdbe.org/1nbl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nbl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbl ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [https://pdbe.org/1nbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [https://www.ebi.ac.uk/pdbsum/1nbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 18:41, 20 November 2019

2019-11-20T18:41:03Z

←Older revision		Revision as of 18:41, 20 November 2019
Line 1:		Line 1:

	==NMR Structure of Hellethionin D==		==NMR Structure of Hellethionin D==
-	<StructureSection load='1nbl' size='340' side='right' caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1nbl' size='340' side='right'caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Helleborus purpurascens]]		[[Category: Helleborus purpurascens]]
		+	[[Category: Large Structures]]
	[[Category: Kerek, F]]		[[Category: Kerek, F]]
	[[Category: Milbradt, A G]]		[[Category: Milbradt, A G]]

OCA at 07:28, 7 February 2018

2018-02-07T07:28:17Z

←Older revision		Revision as of 07:28, 7 February 2018
Line 1:		Line 1:
		+
	==NMR Structure of Hellethionin D==		==NMR Structure of Hellethionin D==
	<StructureSection load='1nbl' size='340' side='right' caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1nbl' size='340' side='right' caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://pdbe.org/1nbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://pdbe.org/1nbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nbl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/1nbl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/1nbl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:36, 8 February 2016

2016-02-08T13:36:46Z

←Older revision		Revision as of 13:36, 8 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nbl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:05, 10 September 2015

2015-09-10T12:05:13Z

←Older revision		Revision as of 12:05, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://pdbe.org/1nbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nbl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:00, 5 January 2015

2015-01-05T16:00:14Z

←Older revision		Revision as of 16:00, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Helleborus purpurascens]]		[[Category: Helleborus purpurascens]]
-	[[Category: Kerek, F.]]	+	[[Category: Kerek, F]]
-	[[Category: Milbradt, A G.]]	+	[[Category: Milbradt, A G]]
-	[[Category: Moroder, L.]]	+	[[Category: Moroder, L]]
-	[[Category: Renner, C.]]	+	[[Category: Renner, C]]
	[[Category: Gamma thionins helleborus]]		[[Category: Gamma thionins helleborus]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 14:36, 28 September 2014

2014-09-28T14:36:53Z

←Older revision		Revision as of 14:36, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1nbl.~~png\|left~~\|200px]]	+	==NMR Structure of Hellethionin D==
		+	<StructureSection load='1nbl' size='340' side='right' caption='[[1nbl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NBL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nbl RCSB], [http://www.ebi.ac.uk/pdbsum/1nbl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/1nbl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thionins are relatively small-sized multiple-cystine peptides that are probably involved in the plant defense against pathogens. As such, these peptides constitute promising candidates for engineered plant resistance in the agricultural industry. More recently, thionins have been proposed as potential immunotoxins in tumor therapy. In the search for pharmacologically active natural products, a new family of thionins was recently discovered in the roots of Helleborus purpurascens that accordingly were termed hellethionins. The structural characterization by NMR of one representative member of this family, i.e., of hellethionin D, clearly reveals that thionins from different sources share a highly conserved overall fold. In fact, the well-defined 3D structure of hellethionin D is very similar to those reported so far for viscotoxins, purothionins, or crambin, although distinct differences could be detected in the C-terminal portion, especially for loop 36-39. These differences may derive from the unusual distribution of charged residues in the C-terminal half of the peptide sequence compared to other thionins and from the uncommon occurrence of four contiguous threonine residues in loop 36-39. As expected, reduction of the disulfide bonds in hellethionin D leads to complete unfolding, but upon oxidative refolding by air oxygen in the presence of glutathione the correct isomer is recovered in high yields, confirming the very robust fold of this class of bioactive cystine peptides.

-	~~{{STRUCTURE_1nbl\| PDB=1nbl \| SCENE= }}~~	+	Structural characterization of hellethionins from Helleborus purpurascens.,Milbradt AG, Kerek F, Moroder L, Renner C Biochemistry. 2003 Mar 4;42(8):2404-11. PMID:12600207<ref>PMID:12600207</ref>

-	~~===NMR Structure~~ of ~~Hellethionin D===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12600207}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1nbl]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Helleborus_purpurascens Helleborus purpurascens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012600207<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Helleborus purpurascens]]		[[Category: Helleborus purpurascens]]
	[[Category: Kerek, F.]]		[[Category: Kerek, F.]]

OCA: Protected "1nbl" [edit=sysop:move=sysop]

2012-12-05T20:13:37Z

Protected "1nbl" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:13, 5 December 2012

OCA at 20:13, 5 December 2012

2012-12-05T20:13:33Z

←Older revision		Revision as of 20:13, 5 December 2012
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-	{{Seed}}
	[[Image:1nbl.png\|left\|200px]]		[[Image:1nbl.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1nbl\| PDB=1nbl \| SCENE= }}		{{STRUCTURE_1nbl\| PDB=1nbl \| SCENE= }}

	===NMR Structure of Hellethionin D===		===NMR Structure of Hellethionin D===

-
-	<!--
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	{{ABSTRACT_PUBMED_12600207}}		{{ABSTRACT_PUBMED_12600207}}

	==About this Structure==		==About this Structure==
-	~~1NBL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA].	+	[[1nbl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helleborus_purpurascens Helleborus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12600207~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012600207</ref><references group="xtra"/>
	[[Category: Helleborus purpurascens]]		[[Category: Helleborus purpurascens]]
	[[Category: Kerek, F.]]		[[Category: Kerek, F.]]
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	[[Category: Renner, C.]]		[[Category: Renner, C.]]
	[[Category: Gamma thionins helleborus]]		[[Category: Gamma thionins helleborus]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 22:43:02 2009''~~	+