1nqn - Revision history

OCA at 08:52, 10 April 2024

2024-04-10T08:52:27Z

←Older revision		Revision as of 08:52, 10 April 2024
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	<StructureSection load='1nqn' size='340' side='right'caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1nqn' size='340' side='right'caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1nqm\|1nqm]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">AVD ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9031 CHICK])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [https://pdbe.org/1nqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [https://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [https://pdbe.org/1nqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [https://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).	+	[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nqn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nqn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The biotin-binding tetrameric proteins, streptavidin from Streptomyces avidinii and chicken egg white avidin, are excellent models for the study of subunit-subunit interactions of a multimeric protein. Efforts are thus being made to prepare mutated forms of streptavidin and avidin, which would form monomers or dimers, in order to examine their effect on quaternary structure and assembly. In the present communication, we compared the crystal structures of binding site W-->K mutations in streptavidin and avidin. In solution, both mutant proteins are known to form dimers, but upon crystallization, both formed tetramers with the same parameters as the native proteins. All of the intersubunit bonds were conserved, except for the hydrophobic interaction between biotin and the tryptophan that was replaced by lysine. In the crystal structure, the binding site of the mutated apo-avidin contains 3 molecules of structured water instead of the 5 contained in the native protein. The lysine side chain extends in a direction opposite that of the native tryptophan, the void being partially filled by an adjacent lysine residue. Nevertheless, the binding-site conformation observed for the mutant tetramer is an artificial consequence of crystal packing that would not be maintained in the solution-phase dimer. It appears that the dimer-tetramer transition may be concentration dependent, and the interaction among subunits obeys the law of mass action.
-
-	Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.,Pazy Y, Eisenberg-Domovich Y, Laitinen OH, Kulomaa MS, Bayer EA, Wilchek M, Livnah O J Bacteriol. 2003 Jul;185(14):4050-6. PMID:12837778<ref>PMID:12837778</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bayer~~, E A~~]]	+	[[Category: Bayer EA]]
-	[[Category: Eisenberg-Domovich, Y]]	+	[[Category: Eisenberg-Domovich Y]]
-	[[Category: Kulomaa~~, M S~~]]	+	[[Category: Kulomaa MS]]
-	[[Category: Laitinen~~, O H~~]]	+	[[Category: Laitinen OH]]
-	[[Category: Livnah, O]]	+	[[Category: Livnah O]]
-	[[Category: Pazy, Y]]	+	[[Category: Pazy Y]]
-	[[Category: Wilchek, M]]	+	[[Category: Wilchek M]]
-	~~[[Category: Avidin]]~~	+
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Monomer-monomer interaction]]~~	+
-	~~[[Category: Streptavidin]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 15:03, 27 October 2021

2021-10-27T15:03:33Z

←Older revision		Revision as of 15:03, 27 October 2021
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	<StructureSection load='1nqn' size='340' side='right'caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1nqn' size='340' side='right'caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1NQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nqm\|1nqm]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nqm\|1nqm]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [~~http~~://pdbe.org/1nqn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [https://pdbe.org/1nqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [https://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).	+	[[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:55, 2 December 2020

2020-12-02T08:55:39Z

←Older revision		Revision as of 08:55, 2 December 2020
Line 1:		Line 1:

	==Structure of Avm-W110K (W110K mutant of avidin)==		==Structure of Avm-W110K (W110K mutant of avidin)==
-	<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1nqn' size='340' side='right'caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nqm\|1nqm]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://pdbe.org/1nqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://pdbe.org/1nqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Eisenberg-Domovich, Y]]		[[Category: Eisenberg-Domovich, Y]]

OCA at 06:25, 31 January 2018

2018-01-31T06:25:24Z

←Older revision		Revision as of 06:25, 31 January 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/1nqn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/1nqn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:02, 1 November 2017

2017-11-01T10:02:37Z

←Older revision		Revision as of 10:02, 1 November 2017
Line 1:		Line 1:
		+
	==Structure of Avm-W110K (W110K mutant of avidin)==		==Structure of Avm-W110K (W110K mutant of avidin)==
	<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://pdbe.org/1nqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://pdbe.org/1nqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nqn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nqn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Avidin\|Avidin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:14, 11 September 2015

2015-09-11T21:14:39Z

←Older revision		Revision as of 21:14, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NQN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 ~~Gallus gallus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://pdbe.org/1nqn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nqn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Bayer, E A]]		[[Category: Bayer, E A]]
	[[Category: Eisenberg-Domovich, Y]]		[[Category: Eisenberg-Domovich, Y]]

OCA at 19:54, 25 December 2014

2014-12-25T19:54:42Z

←Older revision		Revision as of 19:54, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NQN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Bayer, E A.]]	+	[[Category: Bayer, E A]]
-	[[Category: Eisenberg-Domovich, Y.]]	+	[[Category: Eisenberg-Domovich, Y]]
-	[[Category: Kulomaa, M S.]]	+	[[Category: Kulomaa, M S]]
-	[[Category: Laitinen, O H.]]	+	[[Category: Laitinen, O H]]
-	[[Category: Livnah, O.]]	+	[[Category: Livnah, O]]
-	[[Category: Pazy, Y.]]	+	[[Category: Pazy, Y]]
-	[[Category: Wilchek, M.]]	+	[[Category: Wilchek, M]]
	[[Category: Avidin]]		[[Category: Avidin]]
	[[Category: Biotin]]		[[Category: Biotin]]

OCA at 14:06, 28 September 2014

2014-09-28T14:06:38Z

←Older revision		Revision as of 14:06, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1nqn.~~png~~\|~~left~~\|200px]]	+	==Structure of Avm-W110K (W110K mutant of avidin)==
		+	<StructureSection load='1nqn' size='340' side='right' caption='[[1nqn]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NQN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nqm\|1nqm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AVD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nqn RCSB], [http://www.ebi.ac.uk/pdbsum/1nqn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/1nqn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biotin-binding tetrameric proteins, streptavidin from Streptomyces avidinii and chicken egg white avidin, are excellent models for the study of subunit-subunit interactions of a multimeric protein. Efforts are thus being made to prepare mutated forms of streptavidin and avidin, which would form monomers or dimers, in order to examine their effect on quaternary structure and assembly. In the present communication, we compared the crystal structures of binding site W-->K mutations in streptavidin and avidin. In solution, both mutant proteins are known to form dimers, but upon crystallization, both formed tetramers with the same parameters as the native proteins. All of the intersubunit bonds were conserved, except for the hydrophobic interaction between biotin and the tryptophan that was replaced by lysine. In the crystal structure, the binding site of the mutated apo-avidin contains 3 molecules of structured water instead of the 5 contained in the native protein. The lysine side chain extends in a direction opposite that of the native tryptophan, the void being partially filled by an adjacent lysine residue. Nevertheless, the binding-site conformation observed for the mutant tetramer is an artificial consequence of crystal packing that would not be maintained in the solution-phase dimer. It appears that the dimer-tetramer transition may be concentration dependent, and the interaction among subunits obeys the law of mass action.

-	~~{{STRUCTURE_1nqn\| PDB=1nqn \| SCENE= }}~~	+	Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.,Pazy Y, Eisenberg-Domovich Y, Laitinen OH, Kulomaa MS, Bayer EA, Wilchek M, Livnah O J Bacteriol. 2003 Jul;185(14):4050-6. PMID:12837778<ref>PMID:12837778</ref>

-	~~===Structure~~ of ~~Avm-W110K (W110K mutant of avidin)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12837778}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1nqn]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012837778<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Bayer, E A.]]		[[Category: Bayer, E A.]]

OCA at 20:48, 20 October 2012

2012-10-20T20:48:32Z

←Older revision		Revision as of 20:48, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1nqn]] is a 2 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA].	+	[[1nqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQN OCA].

	==See Also==		==See Also==

OCA at 11:41, 27 July 2012

2012-07-27T11:41:12Z

←Older revision		Revision as of 11:41, 27 July 2012
Line 1:		Line 1:
	[[Image:1nqn.png\|left\|200px]]		[[Image:1nqn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1nqn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1nqn\| PDB=1nqn \| SCENE= }}		{{STRUCTURE_1nqn\| PDB=1nqn \| SCENE= }}

	===Structure of Avm-W110K (W110K mutant of avidin)===		===Structure of Avm-W110K (W110K mutant of avidin)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12837778}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12837778 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12837778}}		{{ABSTRACT_PUBMED_12837778}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12837778~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012837778</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Bayer, E A.]]		[[Category: Bayer, E A.]]