1nu3 - Revision history

OCA at 08:53, 10 April 2024

2024-04-10T08:53:20Z

←Older revision		Revision as of 08:53, 10 April 2024
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	<StructureSection load='1nu3' size='340' side='right'caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1nu3' size='340' side='right'caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium~~ erythropolis~~" gray and thornton 1928~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MES:2~~-~~(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>~~, ~~<scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class="sblockDat">limA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [https://pdbe.org/1nu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [https://pdbe.org/1nu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER]] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.	+	[https://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nu3 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nu3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. The classic epoxide hydrolases have an alpha/beta hydrolase fold and act via a two-step reaction mechanism including an enzyme-substrate intermediate. We report here the structure of the limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis, solved using single-wavelength anomalous dispersion from a selenomethionine-substituted protein and refined at 1.2 A resolution. This enzyme represents a completely different structure and a novel one-step mechanism. The fold features a highly curved six-stranded mixed beta-sheet, with four alpha-helices packed onto it to create a deep pocket. Although most residues lining this pocket are hydrophobic, a cluster of polar groups, including an Asp-Arg-Asp triad, interact at its deepest point. Site-directed mutagenesis supports the conclusion that this is the active site. Further, a 1.7 A resolution structure shows the inhibitor valpromide bound at this position, with its polar atoms interacting directly with the residues of the triad. We suggest that several bacterial proteins of currently unknown function will share this structure and, in some cases, catalytic properties.
-
-	Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site.,Arand M, Hallberg BM, Zou J, Bergfors T, Oesch F, van der Werf MJ, de Bont JA, Jones TA, Mowbray SL EMBO J. 2003 Jun 2;22(11):2583-92. PMID:12773375<ref>PMID:12773375</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Epoxide hydrolase 3D structures\|Epoxide hydrolase 3D structures]]		*[[Epoxide hydrolase 3D structures\|Epoxide hydrolase 3D structures]]
	*[[Limonene-1%2C2-epoxide hydrolase\|Limonene-1%2C2-epoxide hydrolase]]		*[[Limonene-1%2C2-epoxide hydrolase\|Limonene-1%2C2-epoxide hydrolase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Mycobacterium erythropolis gray and thornton 1928]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Limonene-1,2-epoxide hydrolase~~]]	+	[[Category: Rhodococcus erythropolis]]
-	[[Category: Arand, M]]	+	[[Category: Arand M]]
-	[[Category: Bergfors, T]]	+	[[Category: Bergfors T]]
-	~~[[Category: Bont, J A.M de~~]]	+	[[Category: Hallberg BM]]
-	[[Category: Hallberg~~, B M~~]]	+	[[Category: Jones TA]]
-	[[Category: Jones~~, T A~~]]	+	[[Category: Mowbray SL]]
-	[[Category: Mowbray~~, S L~~]]	+	[[Category: Oesch F]]
-	[[Category: Oesch, F]]	+	[[Category: Zou J]]
-	~~[[Category: Werf, M J.van der~~]]	+	[[Category: De Bont JAM]]
-	[[Category: Zou, J]]	+	[[Category: Van der Werf MJ]]
-	[[Category: ~~Hydrolase~~]]	+
-	[[Category: ~~Protein-ligand complex~~]]	+

OCA at 06:26, 10 November 2021

2021-11-10T06:26:41Z

OCA at 08:56, 2 December 2020

2020-12-02T08:56:55Z

←Older revision		Revision as of 08:56, 2 December 2020
Line 1:		Line 1:

	==Limonene-1,2-epoxide hydrolase in complex with valpromide==		==Limonene-1,2-epoxide hydrolase in complex with valpromide==
-	<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='1nu3' size='340' side='right'caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://pdbe.org/1nu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://pdbe.org/1nu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Epoxide hydrolase\|Epoxide hydrolase]]	+	*[[Epoxide hydrolase 3D structures\|Epoxide hydrolase 3D structures]]
		+	*[[Limonene-1%2C2-epoxide hydrolase\|Limonene-1%2C2-epoxide hydrolase]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium erythropolis gray and thornton 1928]]		[[Category: Mycobacterium erythropolis gray and thornton 1928]]
		+	[[Category: Large Structures]]
	[[Category: Limonene-1,2-epoxide hydrolase]]		[[Category: Limonene-1,2-epoxide hydrolase]]
	[[Category: Arand, M]]		[[Category: Arand, M]]

OCA at 07:31, 23 May 2018

2018-05-23T07:31:37Z

←Older revision		Revision as of 07:31, 23 May 2018
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/1nu3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/1nu3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Epoxide hydrolase\|Epoxide hydrolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:58, 1 November 2017

2017-11-01T09:58:09Z

←Older revision		Revision as of 09:58, 1 November 2017
Line 1:		Line 1:
		+
	==Limonene-1,2-epoxide hydrolase in complex with valpromide==		==Limonene-1,2-epoxide hydrolase in complex with valpromide==
	<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://pdbe.org/1nu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://pdbe.org/1nu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nu3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Epoxide hydrolase\|Epoxide hydrolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:46, 11 September 2015

2015-09-11T08:46:24Z

←Older revision		Revision as of 08:46, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodococcus_erythropolis Rhodococcus~~ erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 ~~Rhodococcus~~ erythropolis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://pdbe.org/1nu3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1nu3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Epoxide hydrolase\|Epoxide hydrolase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Mycobacterium erythropolis gray and thornton 1928]]
	[[Category: Limonene-1,2-epoxide hydrolase]]		[[Category: Limonene-1,2-epoxide hydrolase]]
-	[[Category: Rhodococcus erythropolis]]
	[[Category: Arand, M]]		[[Category: Arand, M]]
	[[Category: Bergfors, T]]		[[Category: Bergfors, T]]

OCA at 14:03, 25 December 2014

2014-12-25T14:03:02Z

←Older revision		Revision as of 14:03, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER]] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: Limonene-1,2-epoxide hydrolase]]		[[Category: Limonene-1,2-epoxide hydrolase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
-	[[Category: Arand, M.]]	+	[[Category: Arand, M]]
-	[[Category: Bergfors, T.]]	+	[[Category: Bergfors, T]]
-	[[Category: Bont, J A.M de.]]	+	[[Category: Bont, J A.M de]]
-	[[Category: Hallberg, B M.]]	+	[[Category: Hallberg, B M]]
-	[[Category: Jones, T A.]]	+	[[Category: Jones, T A]]
-	[[Category: Mowbray, S L.]]	+	[[Category: Mowbray, S L]]
-	[[Category: Oesch, F.]]	+	[[Category: Oesch, F]]
-	[[Category: Werf, M J.van der.]]	+	[[Category: Werf, M J.van der]]
-	[[Category: Zou, J.]]	+	[[Category: Zou, J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein-ligand complex]]		[[Category: Protein-ligand complex]]

OCA at 16:13, 28 September 2014

2014-09-28T16:13:34Z

←Older revision		Revision as of 16:13, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1nu3.~~png~~\|~~left~~\|200px]]	+	==Limonene-1,2-epoxide hydrolase in complex with valpromide==
		+	<StructureSection load='1nu3' size='340' side='right' caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [http://www.ebi.ac.uk/pdbsum/1nu3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/1nu3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Epoxide hydrolases are essential for the processing of epoxide-containing compounds in detoxification or metabolism. The classic epoxide hydrolases have an alpha/beta hydrolase fold and act via a two-step reaction mechanism including an enzyme-substrate intermediate. We report here the structure of the limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis, solved using single-wavelength anomalous dispersion from a selenomethionine-substituted protein and refined at 1.2 A resolution. This enzyme represents a completely different structure and a novel one-step mechanism. The fold features a highly curved six-stranded mixed beta-sheet, with four alpha-helices packed onto it to create a deep pocket. Although most residues lining this pocket are hydrophobic, a cluster of polar groups, including an Asp-Arg-Asp triad, interact at its deepest point. Site-directed mutagenesis supports the conclusion that this is the active site. Further, a 1.7 A resolution structure shows the inhibitor valpromide bound at this position, with its polar atoms interacting directly with the residues of the triad. We suggest that several bacterial proteins of currently unknown function will share this structure and, in some cases, catalytic properties.

-	~~{{STRUCTURE_1nu3\| PDB=1nu3 \| SCENE= }}~~	+	Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site.,Arand M, Hallberg BM, Zou J, Bergfors T, Oesch F, van der Werf MJ, de Bont JA, Jones TA, Mowbray SL EMBO J. 2003 Jun 2;22(11):2583-92. PMID:12773375<ref>PMID:12773375</ref>

-	~~===Limonene-1~~,~~2-epoxide hydrolase in complex with valpromide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12773375}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1nu3]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012773375<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Limonene-1,2-epoxide hydrolase]]		[[Category: Limonene-1,2-epoxide hydrolase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]

OCA: Protected "1nu3" [edit=sysop:move=sysop]

2012-12-05T21:18:23Z

Protected "1nu3" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:18, 5 December 2012

OCA at 21:18, 5 December 2012

2012-12-05T21:18:20Z

←Older revision		Revision as of 21:18, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1nu3.png\|left\|200px]]		[[Image:1nu3.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1nu3", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1nu3\| PDB=1nu3 \| SCENE= }}		{{STRUCTURE_1nu3\| PDB=1nu3 \| SCENE= }}

	===Limonene-1,2-epoxide hydrolase in complex with valpromide===		===Limonene-1,2-epoxide hydrolase in complex with valpromide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12773375}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12773375 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12773375}}		{{ABSTRACT_PUBMED_12773375}}

	==About this Structure==		==About this Structure==
-	~~1NU3~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA].	+	[[1nu3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12773375~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012773375</ref><references group="xtra"/>
	[[Category: Limonene-1,2-epoxide hydrolase]]		[[Category: Limonene-1,2-epoxide hydrolase]]
	[[Category: Rhodococcus erythropolis]]		[[Category: Rhodococcus erythropolis]]
Line 35:		Line 23:
	[[Category: Werf, M J.van der.]]		[[Category: Werf, M J.van der.]]
	[[Category: Zou, J.]]		[[Category: Zou, J.]]
		+	[[Category: Hydrolase]]
	[[Category: Protein-ligand complex]]		[[Category: Protein-ligand complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:10:06 2009''

←Older revision		Revision as of 06:26, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='1nu3' size='340' side='right'caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1nu3' size='340' side='right'caption='[[1nu3]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1nu3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_erythropolis"_gray_and_thornton_1928 "mycobacterium erythropolis" gray and thornton 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NU3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=VPR:2-PROPYLPENTANAMIDE'>VPR</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">limA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 "Mycobacterium erythropolis" Gray and Thornton 1928])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [~~http~~://pdbe.org/1nu3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nu3 OCA], [https://pdbe.org/1nu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nu3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nu3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER]] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.	+	[[https://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER]] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]