1otv - Revision history

OCA at 08:03, 14 February 2024

2024-02-14T08:03:08Z

←Older revision		Revision as of 08:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1otv' size='340' side='right'caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1otv' size='340' side='right'caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PQQC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~573~~ "~~Bacillus pneumoniae~~" ~~(Schroeter 1886) Flugge 1886~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [https://pdbe.org/1otv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [https://www.ebi.ac.uk/pdbsum/1otv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [https://pdbe.org/1otv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [https://www.ebi.ac.uk/pdbsum/1otv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PQQC_KLEPN PQQC_KLEPN]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.<ref>PMID:7665488</ref>	+	[https://www.uniprot.org/uniprot/PQQC_KLEPN PQQC_KLEPN] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.<ref>PMID:7665488</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1otv ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1otv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The biosynthesis of pyrroloquinoline quinone (PQQ), a vitamin and redox cofactor of quinoprotein dehydrogenases, is facilitated by an unknown pathway that requires the expression of six genes, pqqA to -F. PqqC, the protein encoded by pqqC, catalyzes the final step in the pathway in a reaction that involves ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-d icarboxylic-acid to PQQ. Herein, we describe the crystal structures of PqqC and its complex with PQQ and determine the stoichiometry of H2O2 formation and O2 uptake during the reaction. The PqqC structure(s) reveals a compact seven-helix bundle that provides the scaffold for a positively charged active site cavity. Product binding induces a large conformational change, which results in the active site recruitment of amino acid side chains proposed to play key roles in the catalytic mechanism. PqqC is unusual in that it transfers redox equivalents to molecular oxygen without the assistance of a redox active metal or cofactor. The structure of the enzyme-product complex shows additional electron density next to R179 and C5 of PQQ, which can be modeled as O2 or H2O2, indicating a site for oxygen binding. We propose a reaction sequence that involves base-catalyzed cyclization and a series of quinone-quinol tautomerizations that are followed by cycles of O2/H2O2-mediated oxidations.
-
-	Quinone biogenesis: Structure and mechanism of PqqC, the final catalyst in the production of pyrroloquinoline quinone.,Magnusson OT, Toyama H, Saeki M, Rojas A, Reed JC, Liddington RC, Klinman JP, Schwarzenbacher R Proc Natl Acad Sci U S A. 2004 May 25;101(21):7913-8. Epub 2004 May 17. PMID:15148379<ref>PMID:15148379</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1otv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adachi, O]]	+	[[Category: Adachi O]]
-	[[Category: Klinman~~, J P~~]]	+	[[Category: Klinman JP]]
-	[[Category: Magnusson~~, O T~~]]	+	[[Category: Magnusson OT]]
-	[[Category: Reed~~, J C~~]]	+	[[Category: Reed JC]]
-	[[Category: Rojas, A]]	+	[[Category: Rojas A]]
-	[[Category: SChwarzenbacher, R]]	+	[[Category: SChwarzenbacher R]]
-	[[Category: Saeki, M]]	+	[[Category: Saeki M]]
-	[[Category: Toyama, H]]	+	[[Category: Toyama H]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Pqq biosynthesis enzyme]]~~	+
-	~~[[Category: Seven helix bundle~~]]	+

OCA at 15:08, 27 October 2021

2021-10-27T15:08:36Z

←Older revision		Revision as of 15:08, 27 October 2021
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	<StructureSection load='1otv' size='340' side='right'caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1otv' size='340' side='right'caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1OTV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [~~http~~://pdbe.org/1otv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1otv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [https://pdbe.org/1otv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [https://www.ebi.ac.uk/pdbsum/1otv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PQQC_KLEPN PQQC_KLEPN]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.<ref>PMID:7665488</ref>	+	[[https://www.uniprot.org/uniprot/PQQC_KLEPN PQQC_KLEPN]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.<ref>PMID:7665488</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:15, 9 December 2020

2020-12-09T07:15:17Z

←Older revision		Revision as of 07:15, 9 December 2020
Line 1:		Line 1:

	==PqqC, Pyrroloquinolinquinone Synthase C==		==PqqC, Pyrroloquinolinquinone Synthase C==
-	<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1otv' size='340' side='right'caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OTV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://pdbe.org/1otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://pdbe.org/1otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Adachi, O]]		[[Category: Adachi, O]]
	[[Category: Klinman, J P]]		[[Category: Klinman, J P]]

OCA at 06:25, 31 January 2018

2018-01-31T06:25:58Z

←Older revision		Revision as of 06:25, 31 January 2018
Line 1:		Line 1:
		+
	==PqqC, Pyrroloquinolinquinone Synthase C==		==PqqC, Pyrroloquinolinquinone Synthase C==
	<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://pdbe.org/1otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://pdbe.org/1otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1otv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1otv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1otv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:16, 9 February 2016

2016-02-09T18:16:29Z

←Older revision		Revision as of 18:16, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1otv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:47, 11 September 2015

2015-09-11T00:47:53Z

←Older revision		Revision as of 00:47, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae Klebsiella~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 ~~Klebsiella~~ pneumoniae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://pdbe.org/1otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1otv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Klebsiella pneumoniae]]
	[[Category: Adachi, O]]		[[Category: Adachi, O]]
	[[Category: Klinman, J P]]		[[Category: Klinman, J P]]

OCA at 08:36, 25 December 2014

2014-12-25T08:36:39Z

←Older revision		Revision as of 08:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PQQC_KLEPN PQQC_KLEPN]] Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.<ref>PMID:7665488</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
-	[[Category: Adachi, O.]]	+	[[Category: Adachi, O]]
-	[[Category: Klinman, J P.]]	+	[[Category: Klinman, J P]]
-	[[Category: Magnusson, O T.]]	+	[[Category: Magnusson, O T]]
-	[[Category: Reed, J C.]]	+	[[Category: Reed, J C]]
-	[[Category: Rojas, A.]]	+	[[Category: Rojas, A]]
-	[[Category: SChwarzenbacher, R.]]	+	[[Category: SChwarzenbacher, R]]
-	[[Category: Saeki, M.]]	+	[[Category: Saeki, M]]
-	[[Category: Toyama, H.]]	+	[[Category: Toyama, H]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Pqq biosynthesis enzyme]]		[[Category: Pqq biosynthesis enzyme]]
	[[Category: Seven helix bundle]]		[[Category: Seven helix bundle]]

OCA at 21:31, 28 September 2014

2014-09-28T21:31:47Z

←Older revision		Revision as of 21:31, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1otv.~~png\|left~~\|200px]]	+	==PqqC, Pyrroloquinolinquinone Synthase C==
		+	<StructureSection load='1otv' size='340' side='right' caption='[[1otv]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OTV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PQQC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1otv RCSB], [http://www.ebi.ac.uk/pdbsum/1otv PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1otv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biosynthesis of pyrroloquinoline quinone (PQQ), a vitamin and redox cofactor of quinoprotein dehydrogenases, is facilitated by an unknown pathway that requires the expression of six genes, pqqA to -F. PqqC, the protein encoded by pqqC, catalyzes the final step in the pathway in a reaction that involves ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-d icarboxylic-acid to PQQ. Herein, we describe the crystal structures of PqqC and its complex with PQQ and determine the stoichiometry of H2O2 formation and O2 uptake during the reaction. The PqqC structure(s) reveals a compact seven-helix bundle that provides the scaffold for a positively charged active site cavity. Product binding induces a large conformational change, which results in the active site recruitment of amino acid side chains proposed to play key roles in the catalytic mechanism. PqqC is unusual in that it transfers redox equivalents to molecular oxygen without the assistance of a redox active metal or cofactor. The structure of the enzyme-product complex shows additional electron density next to R179 and C5 of PQQ, which can be modeled as O2 or H2O2, indicating a site for oxygen binding. We propose a reaction sequence that involves base-catalyzed cyclization and a series of quinone-quinol tautomerizations that are followed by cycles of O2/H2O2-mediated oxidations.

-	~~{{STRUCTURE_1otv\| PDB=1otv \| SCENE= }}~~	+	Quinone biogenesis: Structure and mechanism of PqqC, the final catalyst in the production of pyrroloquinoline quinone.,Magnusson OT, Toyama H, Saeki M, Rojas A, Reed JC, Liddington RC, Klinman JP, Schwarzenbacher R Proc Natl Acad Sci U S A. 2004 May 25;101(21):7913-8. Epub 2004 May 17. PMID:15148379<ref>PMID:15148379</ref>

-	~~===PqqC~~, ~~Pyrroloquinolinquinone Synthase C===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15148379}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1otv]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015148379<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
	[[Category: Adachi, O.]]		[[Category: Adachi, O.]]

OCA: Protected "1otv" [edit=sysop:move=sysop]

2012-12-12T21:43:12Z

Protected "1otv" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:43, 12 December 2012

OCA at 21:43, 12 December 2012

2012-12-12T21:43:10Z

←Older revision		Revision as of 21:43, 12 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1otv.png\|left\|200px]]		[[Image:1otv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1otv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1otv\| PDB=1otv \| SCENE= }}		{{STRUCTURE_1otv\| PDB=1otv \| SCENE= }}

	===PqqC, Pyrroloquinolinquinone Synthase C===		===PqqC, Pyrroloquinolinquinone Synthase C===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15148379}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15148379 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15148379}}		{{ABSTRACT_PUBMED_15148379}}

	==About this Structure==		==About this Structure==
-	~~1OTV~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA].	+	[[1otv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTV OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15148379~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015148379</ref><references group="xtra"/>
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
	[[Category: Adachi, O.]]		[[Category: Adachi, O.]]
Line 33:		Line 21:
	[[Category: Saeki, M.]]		[[Category: Saeki, M.]]
	[[Category: Toyama, H.]]		[[Category: Toyama, H.]]
		+	[[Category: Biosynthetic protein]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Pqq biosynthesis enzyme]]		[[Category: Pqq biosynthesis enzyme]]
	[[Category: Seven helix bundle]]		[[Category: Seven helix bundle]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:44:59 2009''