1ow1 - Revision history

OCA at 08:03, 14 February 2024

2024-02-14T08:03:17Z

←Older revision		Revision as of 08:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1ow1' size='340' side='right'caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1ow1' size='340' side='right'caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [https://pdbe.org/1ow1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ow1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [https://pdbe.org/1ow1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ow1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MINT_HUMAN MINT_HUMAN]] May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.<ref>PMID:11331609</ref> <ref>PMID:12374742</ref>	+	[https://www.uniprot.org/uniprot/MINT_HUMAN MINT_HUMAN] May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.<ref>PMID:11331609</ref> <ref>PMID:12374742</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ow1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ow1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Spen proteins regulate the expression of key transcriptional effectors in diverse signaling pathways. They are large proteins characterized by N-terminal RNA-binding motifs and a highly conserved C-terminal SPOC domain. The specific biological role of the SPOC domain (Spen paralog and ortholog C-terminal domain), and hence, the common function of Spen proteins, has been unclear to date. The Spen protein, SHARP (SMRT/HDAC1-associated repressor protein), was identified as a component of transcriptional repression complexes in both nuclear receptor and Notch/RBP-Jkappa signaling pathways. We have determined the 1.8 A crystal structure of the SPOC domain from SHARP. This structure shows that essentially all of the conserved surface residues map to a positively charged patch. Structure-based mutational analysis indicates that this conserved region is responsible for the interaction between SHARP and the universal transcriptional corepressor SMRT/NCoR (silencing mediator for retinoid and thyroid receptors/nuclear receptor corepressor. We demonstrate that this interaction involves a highly conserved acidic motif at the C terminus of SMRT/NCoR. These findings suggest that the conserved function of the SPOC domain is to mediate interaction with SMRT/NCoR corepressors, and that Spen proteins play an essential role in the repression complex.
-
-	A conserved structural motif reveals the essential transcriptional repression function of Spen proteins and their role in developmental signaling.,Ariyoshi M, Schwabe JW Genes Dev. 2003 Aug 1;17(15):1909-20. PMID:12897056<ref>PMID:12897056</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ow1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ariyoshi, M]]	+	[[Category: Ariyoshi M]]
-	[[Category: Schwabe~~, J W]]~~	+	[[Category: Schwabe JW]]
-	~~[[Category: Beta-alpha-barrel]]~~	+
-	~~[[Category: Spoc domain]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 09:22, 8 September 2021

2021-09-08T09:22:10Z

←Older revision		Revision as of 09:22, 8 September 2021
Line 3:		Line 3:
	<StructureSection load='1ow1' size='340' side='right'caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1ow1' size='340' side='right'caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [~~http~~://pdbe.org/1ow1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ow1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow1 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [https://pdbe.org/1ow1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ow1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MINT_HUMAN MINT_HUMAN]] May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.<ref>PMID:11331609</ref> <ref>PMID:12374742</ref>	+	[[https://www.uniprot.org/uniprot/MINT_HUMAN MINT_HUMAN]] May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.<ref>PMID:11331609</ref> <ref>PMID:12374742</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:57, 27 November 2019

2019-11-27T09:57:03Z

←Older revision		Revision as of 09:57, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.==		==Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.==
-	<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1ow1' size='340' side='right'caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ariyoshi, M]]		[[Category: Ariyoshi, M]]
	[[Category: Schwabe, J W]]		[[Category: Schwabe, J W]]

OCA at 07:31, 15 February 2018

2018-02-15T07:31:38Z

←Older revision		Revision as of 07:31, 15 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.==		==Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.==
	<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://pdbe.org/1ow1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://pdbe.org/1ow1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/1ow1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/1ow1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ow1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:23, 12 September 2015

2015-09-12T01:23:35Z

←Older revision		Revision as of 01:23, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://pdbe.org/1ow1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ow1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Ariyoshi, M]]		[[Category: Ariyoshi, M]]
	[[Category: Schwabe, J W]]		[[Category: Schwabe, J W]]

OCA at 21:57, 25 December 2014

2014-12-25T21:57:19Z

←Older revision		Revision as of 21:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MINT_HUMAN MINT_HUMAN]] May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.<ref>PMID:11331609</ref> <ref>PMID:12374742</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Ariyoshi, M.]]	+	[[Category: Ariyoshi, M]]
-	[[Category: Schwabe, J W.]]	+	[[Category: Schwabe, J W]]
	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]
	[[Category: Spoc domain]]		[[Category: Spoc domain]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 23:16, 28 September 2014

2014-09-28T23:16:35Z

←Older revision		Revision as of 23:16, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1ow1.~~png\|left~~\|200px]]	+	==Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.==
		+	<StructureSection load='1ow1' size='340' side='right' caption='[[1ow1]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OW1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ow1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ow1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/1ow1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Spen proteins regulate the expression of key transcriptional effectors in diverse signaling pathways. They are large proteins characterized by N-terminal RNA-binding motifs and a highly conserved C-terminal SPOC domain. The specific biological role of the SPOC domain (Spen paralog and ortholog C-terminal domain), and hence, the common function of Spen proteins, has been unclear to date. The Spen protein, SHARP (SMRT/HDAC1-associated repressor protein), was identified as a component of transcriptional repression complexes in both nuclear receptor and Notch/RBP-Jkappa signaling pathways. We have determined the 1.8 A crystal structure of the SPOC domain from SHARP. This structure shows that essentially all of the conserved surface residues map to a positively charged patch. Structure-based mutational analysis indicates that this conserved region is responsible for the interaction between SHARP and the universal transcriptional corepressor SMRT/NCoR (silencing mediator for retinoid and thyroid receptors/nuclear receptor corepressor. We demonstrate that this interaction involves a highly conserved acidic motif at the C terminus of SMRT/NCoR. These findings suggest that the conserved function of the SPOC domain is to mediate interaction with SMRT/NCoR corepressors, and that Spen proteins play an essential role in the repression complex.

-	~~{{STRUCTURE_1ow1\| PDB=1ow1 \| SCENE= }}~~	+	A conserved structural motif reveals the essential transcriptional repression function of Spen proteins and their role in developmental signaling.,Ariyoshi M, Schwabe JW Genes Dev. 2003 Aug 1;17(15):1909-20. PMID:12897056<ref>PMID:12897056</ref>

-	~~===Crystal structure~~ of the ~~SPOC domain~~ of ~~the human transcriptional corepressor, SHARP~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12897056}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ow1]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012897056<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ariyoshi, M.]]		[[Category: Ariyoshi, M.]]

OCA: Protected "1ow1" [edit=sysop:move=sysop]

2012-12-12T21:52:49Z

Protected "1ow1" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:52, 12 December 2012

OCA at 21:52, 12 December 2012

2012-12-12T21:52:44Z

←Older revision		Revision as of 21:52, 12 December 2012
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-	{{Seed}}
	[[Image:1ow1.png\|left\|200px]]		[[Image:1ow1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ow1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1ow1\| PDB=1ow1 \| SCENE= }}		{{STRUCTURE_1ow1\| PDB=1ow1 \| SCENE= }}

	===Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.===		===Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12897056}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12897056 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12897056}}		{{ABSTRACT_PUBMED_12897056}}

	==About this Structure==		==About this Structure==
-	~~1OW1~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA].	+	[[1ow1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12897056~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012897056</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ariyoshi, M.]]		[[Category: Ariyoshi, M.]]
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	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]
	[[Category: Spoc domain]]		[[Category: Spoc domain]]
-		+	[[Category: Transcription]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 09:07:01 2009''~~	+

OCA at 07:07, 18 February 2009

2009-02-18T07:07:02Z

←Older revision		Revision as of 07:07, 18 February 2009
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	==About this Structure==		==About this Structure==
-	1OW1 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA].	+	1OW1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW1 OCA].

	==Reference==		==Reference==
-	A conserved structural motif reveals the essential transcriptional repression function of Spen proteins and their role in developmental signaling., Ariyoshi M, Schwabe JW, Genes Dev. 2003 Aug 1;17(15):1909-20. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12897056 12897056]~~	+	<ref group="xtra">PMID:12897056</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Ariyoshi, M.]]		[[Category: Ariyoshi, M.]]
	[[Category: Schwabe, J W.]]		[[Category: Schwabe, J W.]]
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	[[Category: Spoc domain]]		[[Category: Spoc domain]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:20:~~47 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:07:01 2009''