1ozf - Revision history

OCA at 09:34, 16 August 2023

2023-08-16T09:34:31Z

←Older revision		Revision as of 09:34, 16 August 2023
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	<StructureSection load='1ozf' size='340' side='right'caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozf' size='340' side='right'caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1OZF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [https://pdbe.org/1ozf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [https://www.ebi.ac.uk/pdbsum/1ozf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [~~http~~://pdbe.org/1ozf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ozf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ILVB_KLEPN ILVB_KLEPN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lyase]]~~	+	[[Category: Duggleby RG]]
-	[[Category: Duggleby~~, R G~~]]	+	[[Category: Guddat LW]]
-	[[Category: Guddat~~, L W~~]]	+	[[Category: Pang SS]]
-	[[Category: Pang~~, S S~~]]	+	[[Category: Schowen RL]]
-	[[Category: Schowen~~, R L]]~~	+
-	~~[[Category: Acetohydroxyacid synthase]]~~	+
-	~~[[Category: Acetolactate synthase]]~~	+
-	~~[[Category: Thiamin diphosphate~~]]	+

OCA at 07:19, 9 December 2020

2020-12-09T07:19:30Z

←Older revision		Revision as of 07:19, 9 December 2020
Line 1:		Line 1:

	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors==		==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors==
-	<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1ozf' size='340' side='right'caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OZF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1OZF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://pdbe.org/1ozf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://pdbe.org/1ozf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Duggleby, R G]]		[[Category: Duggleby, R G]]

OCA at 07:32, 15 February 2018

2018-02-15T07:32:15Z

←Older revision		Revision as of 07:32, 15 February 2018
Line 1:		Line 1:
		+
	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors==		==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors==
	<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://pdbe.org/1ozf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://pdbe.org/1ozf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:49, 9 February 2016

2016-02-09T05:49:28Z

←Older revision		Revision as of 05:49, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:13, 10 September 2015

2015-09-10T19:13:59Z

←Older revision		Revision as of 19:13, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae Klebsiella~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 ~~Klebsiella~~ pneumoniae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Acetolactate_synthase Acetolactate synthase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.2~~.1.~~6 2~~.2.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.18 4.1.3.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://pdbe.org/1ozf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ozf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acetolactate synthase]]~~	+	[[Category: Lyase]]
-	~~[[Category: Klebsiella pneumoniae~~]]	+
	[[Category: Duggleby, R G]]		[[Category: Duggleby, R G]]
	[[Category: Guddat, L W]]		[[Category: Guddat, L W]]
Line 37:		Line 37:
	[[Category: Schowen, R L]]		[[Category: Schowen, R L]]
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Acetolactate synthase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

OCA at 06:50, 6 January 2015

2015-01-06T06:50:54Z

←Older revision		Revision as of 06:50, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
-	[[Category: Duggleby, R G.]]	+	[[Category: Duggleby, R G]]
-	[[Category: Guddat, L W.]]	+	[[Category: Guddat, L W]]
-	[[Category: Pang, S S.]]	+	[[Category: Pang, S S]]
-	[[Category: Schowen, R L.]]	+	[[Category: Schowen, R L]]
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
-	[[Category: Acetolactate synthase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

OCA at 22:17, 28 September 2014

2014-09-28T22:17:07Z

←Older revision		Revision as of 22:17, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ozf.~~png~~\|~~left~~\|200px]]	+	==The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors==
		+	<StructureSection load='1ozf' size='340' side='right' caption='[[1ozf]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OZF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BUDB OR ILVK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ozf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ozf RCSB], [http://www.ebi.ac.uk/pdbsum/1ozf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetohydroxyacid synthase (AHAS) and acetolactate synthase (ALS) are thiamine diphosphate (ThDP)-dependent enzymes that catalyze the decarboxylation of pyruvate to give a cofactor-bound hydroxyethyl group, which is transferred to a second molecule of pyruvate to give 2-acetolactate. AHAS is found in plants, fungi, and bacteria, is involved in the biosynthesis of the branched-chain amino acids, and contains non-catalytic FAD. ALS is found only in some bacteria, is a catabolic enzyme required for the butanediol fermentation, and does not contain FAD. Here we report the 2.3-A crystal structure of Klebsiella pneumoniae ALS. The overall structure is similar to AHAS except for a groove that accommodates FAD in AHAS, which is filled with amino acid side chains in ALS. The ThDP cofactor has an unusual conformation that is unprecedented among the 26 known three-dimensional structures of nine ThDP-dependent enzymes, including AHAS. This conformation suggests a novel mechanism for ALS. A second structure, at 2.0 A, is described in which the enzyme is trapped halfway through the catalytic cycle so that it contains the hydroxyethyl intermediate bound to ThDP. The cofactor has a tricyclic structure that has not been observed previously in any ThDP-dependent enzyme, although similar structures are well known for free thiamine. This structure is consistent with our proposed mechanism and probably results from an intramolecular proton transfer within a tricyclic carbanion that is the true reaction intermediate. Modeling of the second molecule of pyruvate into the active site of the enzyme with the bound intermediate is consistent with the stereochemistry and specificity of ALS.

-	~~{{STRUCTURE_1ozf\| PDB=1ozf \| SCENE= }}~~	+	The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.,Pang SS, Duggleby RG, Schowen RL, Guddat LW J Biol Chem. 2004 Jan 16;279(3):2242-53. Epub 2003 Oct 13. PMID:14557277<ref>PMID:14557277</ref>

-	~~===The crystal structure~~ of ~~Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14557277}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ozf]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014557277<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]

OCA: Protected "1ozf" [edit=sysop:move=sysop]

2012-12-12T21:53:27Z

Protected "1ozf" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:53, 12 December 2012

OCA at 21:53, 12 December 2012

2012-12-12T21:53:25Z

←Older revision		Revision as of 21:53, 12 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ozf.png\|left\|200px]]		[[Image:1ozf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ozf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ozf\| PDB=1ozf \| SCENE= }}		{{STRUCTURE_1ozf\| PDB=1ozf \| SCENE= }}

	===The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors===		===The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14557277}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14557277 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14557277}}		{{ABSTRACT_PUBMED_14557277}}

	==About this Structure==		==About this Structure==
-	~~1OZF~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA].	+	[[1ozf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14557277~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014557277</ref><references group="xtra"/>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
Line 32:		Line 20:
	[[Category: Acetohydroxyacid synthase]]		[[Category: Acetohydroxyacid synthase]]
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
		+	[[Category: Lyase]]
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:58:14 2009''

OCA at 04:58, 17 February 2009

2009-02-17T04:58:15Z

←Older revision		Revision as of 04:58, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1OZF is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA].	+	1OZF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZF OCA].

	==Reference==		==Reference==
-	The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate., Pang SS, Duggleby RG, Schowen RL, Guddat LW, J Biol Chem. 2004 Jan 16;279(3):2242-53. Epub 2003 Oct 13. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14557277 14557277]~~	+	<ref group="xtra">PMID:14557277</ref><references group="xtra"/>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
-	[[Category: Single protein]]
	[[Category: Duggleby, R G.]]		[[Category: Duggleby, R G.]]
	[[Category: Guddat, L W.]]		[[Category: Guddat, L W.]]
Line 35:		Line 34:
	[[Category: Thiamin diphosphate]]		[[Category: Thiamin diphosphate]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 10~~:37:~~44 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:58:14 2009''