1pv6 - Revision history

OCA at 18:15, 29 May 2024

2024-05-29T18:15:12Z

←Older revision		Revision as of 18:15, 29 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure at 3.5 angstroms of the Escherichia coli lactose permease, an intensively studied member of the major facilitator superfamily of transporters. The molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease. A large internal hydrophilic cavity open to the cytoplasmic side represents the inward-facing conformation of the transporter. The structure with a bound lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major roles in substrate recognition and proton translocation are identified. We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data.
		+
		+	Structure and mechanism of the lactose permease of Escherichia coli.,Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S Science. 2003 Aug 1;301(5633):610-5. PMID:12893935<ref>PMID:12893935</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lactose Permease\|Lactose Permease]]		*[[Lactose Permease\|Lactose Permease]]
	*[[Symporter 3D structures\|Symporter 3D structures]]		*[[Symporter 3D structures\|Symporter 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:59, 17 April 2024

2024-04-17T05:59:35Z

←Older revision		Revision as of 05:59, 17 April 2024
Line 3:		Line 3:
	<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1pv7~~\|~~1pv7~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [https://pdbe.org/1pv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [https://pdbe.org/1pv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).	+	[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure at 3.5 angstroms of the Escherichia coli lactose permease, an intensively studied member of the major facilitator superfamily of transporters. The molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease. A large internal hydrophilic cavity open to the cytoplasmic side represents the inward-facing conformation of the transporter. The structure with a bound lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major roles in substrate recognition and proton translocation are identified. We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data.
-
-	Structure and mechanism of the lactose permease of Escherichia coli.,Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S Science. 2003 Aug 1;301(5633):610-5. PMID:12893935<ref>PMID:12893935</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lactose Permease\|Lactose Permease]]		*[[Lactose Permease\|Lactose Permease]]
	*[[Symporter 3D structures\|Symporter 3D structures]]		*[[Symporter 3D structures\|Symporter 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abramson, J]]	+	[[Category: Abramson J]]
-	[[Category: Iwata, S]]	+	[[Category: Iwata S]]
-	[[Category: Kaback~~, H R~~]]	+	[[Category: Kaback HR]]
-	[[Category: Kasho, V]]	+	[[Category: Kasho V]]
-	[[Category: Smirnova, I]]	+	[[Category: Smirnova I]]
-	[[Category: Verner, G]]	+	[[Category: Verner G]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Sugar transport]]~~	+
-	~~[[Category: Symport]]~~	+
-	~~[[Category: Transport]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 09:35, 8 September 2021

2021-09-08T09:35:20Z

←Older revision		Revision as of 09:35, 8 September 2021
Line 3:		Line 3:
	<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pv7\|1pv7]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [~~http~~://pdbe.org/1pv6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [https://pdbe.org/1pv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).	+	[[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[Lactose Permease\|Lactose Permease]]		*[[Lactose Permease\|Lactose Permease]]
-	*[[Symporter\|Symporter]]	+	*[[Symporter 3D structures\|Symporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:09, 27 November 2019

2019-11-27T10:09:00Z

←Older revision		Revision as of 10:09, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of lactose permease==		==Crystal structure of lactose permease==
-	<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lactose Permease\|Lactose Permease]]
		+	*[[Symporter\|Symporter]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Abramson, J]]		[[Category: Abramson, J]]
	[[Category: Iwata, S]]		[[Category: Iwata, S]]

OCA at 08:09, 24 February 2018

2018-02-24T08:09:32Z

←Older revision		Revision as of 08:09, 24 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of lactose permease==		==Crystal structure of lactose permease==
	<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://pdbe.org/1pv6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://pdbe.org/1pv6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pv6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pv6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Lactose Permease\|Lactose Permease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:32, 10 February 2016

2016-02-10T01:32:34Z

←Older revision		Revision as of 01:32, 10 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:02, 11 September 2015

2015-09-11T04:02:34Z

←Older revision		Revision as of 04:02, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://pdbe.org/1pv6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Abramson, J]]		[[Category: Abramson, J]]
	[[Category: Iwata, S]]		[[Category: Iwata, S]]

OCA at 10:52, 25 December 2014

2014-12-25T10:52:24Z

←Older revision		Revision as of 10:52, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI]] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Abramson, J.]]	+	[[Category: Abramson, J]]
-	[[Category: Iwata, S.]]	+	[[Category: Iwata, S]]
-	[[Category: Kaback, H R.]]	+	[[Category: Kaback, H R]]
-	[[Category: Kasho, V.]]	+	[[Category: Kasho, V]]
-	[[Category: Smirnova, I.]]	+	[[Category: Smirnova, I]]
-	[[Category: Verner, G.]]	+	[[Category: Verner, G]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Sugar transport]]		[[Category: Sugar transport]]

OCA at 23:55, 28 September 2014

2014-09-28T23:55:55Z

←Older revision		Revision as of 23:55, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1pv6.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of lactose permease==
		+	<StructureSection load='1pv6' size='340' side='right' caption='[[1pv6]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pv7\|1pv7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [http://www.ebi.ac.uk/pdbsum/1pv6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pv6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure at 3.5 angstroms of the Escherichia coli lactose permease, an intensively studied member of the major facilitator superfamily of transporters. The molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease. A large internal hydrophilic cavity open to the cytoplasmic side represents the inward-facing conformation of the transporter. The structure with a bound lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major roles in substrate recognition and proton translocation are identified. We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data.

-	~~{{STRUCTURE_1pv6\| PDB=1pv6 \| SCENE= }}~~	+	Structure and mechanism of the lactose permease of Escherichia coli.,Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S Science. 2003 Aug 1;301(5633):610-5. PMID:12893935<ref>PMID:12893935</ref>

-	~~===Crystal structure~~ of ~~lactose permease===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12893935}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1pv6]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA].~~	+

	==See Also==		==See Also==
	*[[Lactose Permease\|Lactose Permease]]		*[[Lactose Permease\|Lactose Permease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012893935<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Abramson, J.]]		[[Category: Abramson, J.]]

OCA at 15:50, 20 October 2012

2012-10-20T15:50:55Z

←Older revision		Revision as of 15:50, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1pv6]] is a 2 chain structure ~~of [[Lactose Permease]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA].	+	[[1pv6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA].

	==See Also==		==See Also==