1q19 - Revision history

OCA at 09:54, 16 August 2023

2023-08-16T09:54:34Z

←Older revision		Revision as of 09:54, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='1q19' size='340' side='right'caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1q19' size='340' side='right'caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q15\|1q15]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [https://pdbe.org/1q19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [https://www.ebi.ac.uk/pdbsum/1q19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q19 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [https://pdbe.org/1q19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [https://www.ebi.ac.uk/pdbsum/1q19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q19 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CARA_PECCA CARA_PECCA]~~] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>	+	[https://www.uniprot.org/uniprot/CARA_PECCC CARA_PECCC] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus carotovorus jones 1901]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gerratana, B~~]]	+	[[Category: Pectobacterium carotovorum]]
-	[[Category: ~~Miller, M T~~]]	+	[[Category: Gerratana B]]
-	[[Category: ~~Rosenzweig, A C~~]]	+	[[Category: Miller MT]]
-	[[Category: ~~Stapon, A~~]]	+	[[Category: Rosenzweig AC]]
-	[[Category: ~~Townsend, C~~ A]]	+	[[Category: Stapon A]]
-	[[Category: ~~Amp-cpp]]~~	+	[[Category: Townsend CA]]
-	~~[[Category: As-b]]~~	+
-	~~[[Category: B-l]]~~	+
-	~~[[Category: B-lactam synthetase]]~~	+
-	~~[[Category: B-methyleneadenosine 5-triphosphate]]~~	+
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Cea]]~~	+
-	~~[[Category: Class b asparagine synthetase]]~~	+
-	~~[[Category: Cma]]~~	+
-	~~[[Category: Cmpr]]~~	+
-	~~[[Category: Some~~]]	+

OCA at 09:37, 8 September 2021

2021-09-08T09:37:14Z

←Older revision		Revision as of 09:37, 8 September 2021
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	<StructureSection load='1q19' size='340' side='right'caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1q19' size='340' side='right'caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q15\|1q15]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [~~http~~://pdbe.org/1q19 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1q19 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1q19 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [https://pdbe.org/1q19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [https://www.ebi.ac.uk/pdbsum/1q19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q19 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CARA_PECCA CARA_PECCA]] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>	+	[[https://www.uniprot.org/uniprot/CARA_PECCA CARA_PECCA]] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:13, 27 November 2019

2019-11-27T10:13:43Z

←Older revision		Revision as of 10:13, 27 November 2019
Line 1:		Line 1:

	==Carbapenam Synthetase==		==Carbapenam Synthetase==
-	<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1q19' size='340' side='right'caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus carotovorus jones 1901]]		[[Category: Bacillus carotovorus jones 1901]]
		+	[[Category: Large Structures]]
	[[Category: Gerratana, B]]		[[Category: Gerratana, B]]
	[[Category: Miller, M T]]		[[Category: Miller, M T]]

OCA at 08:14, 24 February 2018

2018-02-24T08:14:15Z

←Older revision		Revision as of 08:14, 24 February 2018
Line 1:		Line 1:
		+
	==Carbapenam Synthetase==		==Carbapenam Synthetase==
	<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://pdbe.org/1q19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://pdbe.org/1q19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q19 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q19_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q19_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q19 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 40:		Line 41:
	[[Category: Amp-cpp]]		[[Category: Amp-cpp]]
	[[Category: As-b]]		[[Category: As-b]]
-	[[Category: B-lactam synthetase]]
	[[Category: B-l]]		[[Category: B-l]]
		+	[[Category: B-lactam synthetase]]
	[[Category: B-methyleneadenosine 5-triphosphate]]		[[Category: B-methyleneadenosine 5-triphosphate]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
Line 48:		Line 49:
	[[Category: Cma]]		[[Category: Cma]]
	[[Category: Cmpr]]		[[Category: Cmpr]]
		+	[[Category: Some]]

OCA at 07:51, 11 September 2015

2015-09-11T07:51:19Z

←Older revision		Revision as of 07:51, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pectobacterium_carotovorum Pectobacterium carotovorum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_carotovorus"_jones_1901 "bacillus carotovorus" jones 1901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://pdbe.org/1q19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9XB61_ERWCA Q9XB61_ERWCA~~]] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>	+	[[http://www.uniprot.org/uniprot/CARA_PECCA CARA_PECCA]] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1q19" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pectobacterium carotovorum~~]]	+	[[Category: Bacillus carotovorus jones 1901]]
	[[Category: Gerratana, B]]		[[Category: Gerratana, B]]
	[[Category: Miller, M T]]		[[Category: Miller, M T]]

OCA at 13:28, 25 December 2014

2014-12-25T13:28:41Z

←Older revision		Revision as of 13:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9XB61_ERWCA Q9XB61_ERWCA]] Involved in the biosynthesis of carbapenam-3-carboxylate, a beta-lactam antibiotic of the carbapenem class. Catalyzes the ATP-dependent formation of (3S,5S)-carbapenam-3-carboxylate from (2S,5S)-5-carboxymethylproline.<ref>PMID:12820893</ref> <ref>PMID:17658887</ref> <ref>PMID:19371088</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Pectobacterium carotovorum]]		[[Category: Pectobacterium carotovorum]]
-	[[Category: Gerratana, B.]]	+	[[Category: Gerratana, B]]
-	[[Category: Miller, M T.]]	+	[[Category: Miller, M T]]
-	[[Category: Rosenzweig, A C.]]	+	[[Category: Rosenzweig, A C]]
-	[[Category: Stapon, A.]]	+	[[Category: Stapon, A]]
-	[[Category: Townsend, C A.]]	+	[[Category: Townsend, C A]]
	[[Category: Amp-cpp]]		[[Category: Amp-cpp]]
	[[Category: As-b]]		[[Category: As-b]]

OCA at 16:04, 29 September 2014

2014-09-29T16:04:09Z

←Older revision		Revision as of 16:04, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1q19.~~png~~\|~~left~~\|200px]]	+	==Carbapenam Synthetase==
		+	<StructureSection load='1q19' size='340' side='right' caption='[[1q19]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q19 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSC:(2S,5S)-5-CARBOXYMETHYLPROLINE'>SSC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q15\|1q15]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q19 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q19 RCSB], [http://www.ebi.ac.uk/pdbsum/1q19 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q19_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carbapenam synthetase (CarA) is an ATP/Mg2+-dependent enzyme that catalyzes formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis. CarA is homologous to beta-lactam synthetase (beta-LS), which is involved in clavulanic acid biosynthesis. The catalytic cycles of CarA and beta-LS mediate substrate adenylation followed by beta-lactamization via a tetrahedral intermediate or transition state. Another member of this family of ATP/Mg2+-dependent enzymes, asparagine synthetase (AS-B), catalyzes intermolecular, rather than intramolecular, amide bond formation in asparagine biosynthesis. The crystal structures of apo-CarA and CarA complexed with the substrate (2S,5S)-5-carboxymethylproline (CMPr), ATP analog alpha,beta-methyleneadenosine 5'-triphosphate (AMP-CPP), and a single Mg2+ ion have been determined. CarA forms a tetramer. Each monomer resembles beta-LS and AS-B in overall fold, but key differences are observed. The N-terminal domain lacks the glutaminase active site found in AS-B, and an extended loop region not observed in beta-LS or AS-B is present. Comparison of the C-terminal synthetase active site to that in beta-LS reveals that the ATP binding site is highly conserved. By contrast, variations in the substrate binding pocket reflect the different substrates of the two enzymes. The Mg2+ coordination is also different. Several key residues in the active site are conserved between CarA and beta-LS, supporting proposed roles in beta-lactam formation. These data provide further insight into the structures of this class of enzymes and suggest that CarA might be a versatile target for protein engineering experiments aimed at developing improved production methods and new carbapenem antibiotics.

-	~~{{STRUCTURE_1q19\| PDB=1q19 \| SCENE= }}~~	+	Crystal structure of carbapenam synthetase (CarA).,Miller MT, Gerratana B, Stapon A, Townsend CA, Rosenzweig AC J Biol Chem. 2003 Oct 17;278(42):40996-1002. Epub 2003 Jul 30. PMID:12890666<ref>PMID:12890666</ref>

-	~~===Carbapenam Synthetase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12890666}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1q19]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012890666<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pectobacterium carotovorum]]		[[Category: Pectobacterium carotovorum]]
	[[Category: Gerratana, B.]]		[[Category: Gerratana, B.]]

OCA: Protected "1q19" [edit=sysop:move=sysop]

2013-01-02T09:46:53Z

Protected "1q19" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:46, 2 January 2013

OCA at 09:46, 2 January 2013

2013-01-02T09:46:49Z

←Older revision		Revision as of 09:46, 2 January 2013
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-	{{Seed}}
	[[Image:1q19.png\|left\|200px]]		[[Image:1q19.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1q19", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1q19\| PDB=1q19 \| SCENE= }}		{{STRUCTURE_1q19\| PDB=1q19 \| SCENE= }}

	===Carbapenam Synthetase===		===Carbapenam Synthetase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12890666}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12890666 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12890666}}		{{ABSTRACT_PUBMED_12890666}}

	==About this Structure==		==About this Structure==
-	~~1Q19~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA].	+	[[1q19]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12890666~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012890666</ref><references group="xtra"/>
	[[Category: Pectobacterium carotovorum]]		[[Category: Pectobacterium carotovorum]]
	[[Category: Gerratana, B.]]		[[Category: Gerratana, B.]]
Line 30:		Line 18:
	[[Category: Stapon, A.]]		[[Category: Stapon, A.]]
	[[Category: Townsend, C A.]]		[[Category: Townsend, C A.]]
-	[[Category: A,b-methyleneadenosine 5-triphosphate]]
	[[Category: Amp-cpp]]		[[Category: Amp-cpp]]
	[[Category: As-b]]		[[Category: As-b]]
	[[Category: B-lactam synthetase]]		[[Category: B-lactam synthetase]]
	[[Category: B-l]]		[[Category: B-l]]
		+	[[Category: B-methyleneadenosine 5-triphosphate]]
		+	[[Category: Biosynthetic protein]]
	[[Category: Cea]]		[[Category: Cea]]
	[[Category: Class b asparagine synthetase]]		[[Category: Class b asparagine synthetase]]
	[[Category: Cma]]		[[Category: Cma]]
	[[Category: Cmpr]]		[[Category: Cmpr]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:25:56 2009''

OCA at 15:25, 17 February 2009

2009-02-17T15:25:57Z

←Older revision		Revision as of 15:25, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1Q19 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA].	+	1Q19 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q19 OCA].

	==Reference==		==Reference==
-	~~Crystal structure of carbapenam synthetase (CarA)., Miller MT, Gerratana B, Stapon A, Townsend CA, Rosenzweig AC, J Biol Chem. 2003 Oct 17;278(42):40996-1002. Epub 2003 Jul 30.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12890666 12890666]~~	+	<ref group="xtra">PMID:12890666</ref><references group="xtra"/>
	[[Category: Pectobacterium carotovorum]]		[[Category: Pectobacterium carotovorum]]
-	[[Category: Single protein]]
	[[Category: Gerratana, B.]]		[[Category: Gerratana, B.]]
	[[Category: Miller, M T.]]		[[Category: Miller, M T.]]
Line 41:		Line 40:
	[[Category: Cmpr]]		[[Category: Cmpr]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 00~~:24:~~20 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:25:56 2009''