1q2j - Revision history

OCA at 12:36, 10 January 2024

2024-01-10T12:36:21Z

←Older revision		Revision as of 12:36, 10 January 2024
Line 1:		Line 1:

	==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==		==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==
-	<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_stercusmuscarum Conus stercusmuscarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [https://pdbe.org/1q2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [https://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [https://pdbe.org/1q2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [https://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CM3A_CONSE CM3A_CONSE]] Mu-conotoxins block voltage-gated sodium channels (Nav). This toxin blocks rNav1.5/SCN5A (IC(50) is 1.3 uM), rNav1.6/SCN8A (IC(50) is 160 nM), rNav1.7/SCN9A (IC(50) is 1.3 uM), rNav1.1/SCN1A (K(d) is 3.8 nM), rNav1.2/SCN2A (K(d) is 1.3 nM), rNav1.4/SCN4A (K(d) is 0.22 nM), rNav1.6/SCN8A (K(d) is 69 nM), and rNav1.7/SCN9A (K(d) is 260 nM). This toxin is very potent but weakly discriminating among sodium channels. The block of these channels is modified when beta-subunits are coexpressed with alpha subunits. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits).<ref>PMID:12484778</ref> <ref>PMID:21652775</ref> <ref>PMID:22229737</ref> <ref>PMID:23146020</ref>	+	[https://www.uniprot.org/uniprot/CM3A_CONSE CM3A_CONSE] Mu-conotoxins block voltage-gated sodium channels (Nav). This toxin blocks rNav1.5/SCN5A (IC(50) is 1.3 uM), rNav1.6/SCN8A (IC(50) is 160 nM), rNav1.7/SCN9A (IC(50) is 1.3 uM), rNav1.1/SCN1A (K(d) is 3.8 nM), rNav1.2/SCN2A (K(d) is 1.3 nM), rNav1.4/SCN4A (K(d) is 0.22 nM), rNav1.6/SCN8A (K(d) is 69 nM), and rNav1.7/SCN9A (K(d) is 260 nM). This toxin is very potent but weakly discriminating among sodium channels. The block of these channels is modified when beta-subunits are coexpressed with alpha subunits. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits).<ref>PMID:12484778</ref> <ref>PMID:21652775</ref> <ref>PMID:22229737</ref> <ref>PMID:23146020</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Conus stercusmuscarum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bulaj, G]]	+	[[Category: Bulaj G]]
-	[[Category: Keizer~~, D W~~]]	+	[[Category: Keizer DW]]
-	[[Category: Lee~~, E F~~]]	+	[[Category: Lee EF]]
-	[[Category: Norton~~, R S~~]]	+	[[Category: Norton RS]]
-	[[Category: Olivera~~, B M~~]]	+	[[Category: Olivera BM]]
-	[[Category: West~~, P J~~]]	+	[[Category: West PJ]]
-	[[Category: Yoshikami, D]]	+	[[Category: Yoshikami D]]
-	~~[[Category: Mu-conotoxin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:51, 6 April 2022

2022-04-06T10:51:38Z

←Older revision		Revision as of 10:51, 6 April 2022
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	<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [~~http~~://pdbe.org/1q2j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [https://pdbe.org/1q2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [https://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CM3A_CONSE CM3A_CONSE]] Mu-conotoxins block voltage-gated sodium channels (Nav). This toxin blocks rNav1.5/SCN5A (IC(50) is 1.3 uM), rNav1.6/SCN8A (IC(50) is 160 nM), rNav1.7/SCN9A (IC(50) is 1.3 uM), rNav1.1/SCN1A (K(d) is 3.8 nM), rNav1.2/SCN2A (K(d) is 1.3 nM), rNav1.4/SCN4A (K(d) is 0.22 nM), rNav1.6/SCN8A (K(d) is 69 nM), and rNav1.7/SCN9A (K(d) is 260 nM). This toxin is very potent but weakly discriminating among sodium channels. The block of these channels is modified when beta-subunits are coexpressed with alpha subunits. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits).<ref>PMID:12484778</ref> <ref>PMID:21652775</ref> <ref>PMID:22229737</ref> <ref>PMID:23146020</ref>	+	[[https://www.uniprot.org/uniprot/CM3A_CONSE CM3A_CONSE]] Mu-conotoxins block voltage-gated sodium channels (Nav). This toxin blocks rNav1.5/SCN5A (IC(50) is 1.3 uM), rNav1.6/SCN8A (IC(50) is 160 nM), rNav1.7/SCN9A (IC(50) is 1.3 uM), rNav1.1/SCN1A (K(d) is 3.8 nM), rNav1.2/SCN2A (K(d) is 1.3 nM), rNav1.4/SCN4A (K(d) is 0.22 nM), rNav1.6/SCN8A (K(d) is 69 nM), and rNav1.7/SCN9A (K(d) is 260 nM). This toxin is very potent but weakly discriminating among sodium channels. The block of these channels is modified when beta-subunits are coexpressed with alpha subunits. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits).<ref>PMID:12484778</ref> <ref>PMID:21652775</ref> <ref>PMID:22229737</ref> <ref>PMID:23146020</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:36, 25 June 2020

2020-06-25T06:36:29Z

←Older revision		Revision as of 06:36, 25 June 2020
Line 3:		Line 3:
	<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://pdbe.org/1q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://pdbe.org/1q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CM3A_CONSE CM3A_CONSE]] Mu-conotoxins block voltage-gated sodium channels (Nav). This toxin blocks rNav1.5/SCN5A (IC(50) is 1.3 uM), rNav1.6/SCN8A (IC(50) is 160 nM), rNav1.7/SCN9A (IC(50) is 1.3 uM), rNav1.1/SCN1A (K(d) is 3.8 nM), rNav1.2/SCN2A (K(d) is 1.3 nM), rNav1.4/SCN4A (K(d) is 0.22 nM), rNav1.6/SCN8A (K(d) is 69 nM), and rNav1.7/SCN9A (K(d) is 260 nM). This toxin is very potent but weakly discriminating among sodium channels. The block of these channels is modified when beta-subunits are coexpressed with alpha subunits. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits).<ref>PMID:12484778</ref> <ref>PMID:21652775</ref> <ref>PMID:22229737</ref> <ref>PMID:23146020</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:46, 27 November 2019

2019-11-27T09:46:34Z

←Older revision		Revision as of 09:46, 27 November 2019
Line 1:		Line 1:

	==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==		==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==
-	<StructureSection load='1q2j' size='340' side='right' caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1q2j' size='340' side='right'caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bulaj, G]]		[[Category: Bulaj, G]]
	[[Category: Keizer, D W]]		[[Category: Keizer, D W]]

OCA at 07:53, 24 February 2018

2018-02-24T07:53:21Z

←Older revision		Revision as of 07:53, 24 February 2018
Line 1:		Line 1:
		+
	==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==		==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==
	<StructureSection load='1q2j' size='340' side='right' caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1q2j' size='340' side='right' caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://pdbe.org/1q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://pdbe.org/1q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:00, 11 September 2015

2015-09-11T15:00:36Z

←Older revision		Revision as of 15:00, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://pdbe.org/1q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1q2j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:07, 15 January 2015

2015-01-15T16:07:24Z

←Older revision		Revision as of 16:07, 15 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bulaj, G.]]	+	[[Category: Bulaj, G]]
-	[[Category: Keizer, D W.]]	+	[[Category: Keizer, D W]]
-	[[Category: Lee, E F.]]	+	[[Category: Lee, E F]]
-	[[Category: Norton, R S.]]	+	[[Category: Norton, R S]]
-	[[Category: Olivera, B M.]]	+	[[Category: Olivera, B M]]
-	[[Category: West, P J.]]	+	[[Category: West, P J]]
-	[[Category: Yoshikami, D.]]	+	[[Category: Yoshikami, D]]
	[[Category: Mu-conotoxin]]		[[Category: Mu-conotoxin]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 08:17, 8 October 2014

2014-10-08T08:17:01Z

←Older revision		Revision as of 08:17, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1q2j.~~png~~\|~~left\|200px~~]]	+	==Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA==
		+	<StructureSection load='1q2j' size='340' side='right' caption='[[1q2j]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2J FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q2j RCSB], [http://www.ebi.ac.uk/pdbsum/1q2j PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SmIIIA is a new micro-conotoxin isolated recently from Conus stercusmuscarum. Although it shares several biochemical characteristics with other micro-conotoxins (the arrangement of cysteine residues and a conserved arginine believed to interact with residues near the channel pore), it has several distinctive features, including the absence of hydroxyproline, and is the first specific antagonist of tetrodotoxin-resistant voltage-gated sodium channels to be characterized. It therefore represents a potentially useful tool to investigate the functional roles of these channels. We have determined the three-dimensional structure of SmIIIA in aqueous solution. Consistent with the absence of hydroxyprolines, SmIIIA adopts a single conformation with all peptide bonds in the trans configuration. The spatial orientations of several conserved Arg and Lys side chains, including Arg14 (using a consensus numbering system), which plays a key role in sodium channel binding, are similar to those in other micro-conotoxins but the N-terminal regions differ, reflecting the trans conformation for the peptide bond preceding residue 8 in SmIIIA, as opposed to the cis conformation in micro-conotoxins GIIIA and GIIIB. Comparison of the surfaces of SmIIIA with other micro-conotoxins suggests that the affinity of SmIIIA for TTX-resistant channels is influenced by the Trp15 side chain, which is unique to SmIIIA. Arg17, which replaces Lys in the other micro-conotoxins, may also be important. Consistent with these inferences from the structure, assays of two chimeras of SmIIIA and PIIIA in which their N- and C-terminal halves were recombined, indicated that residues in the C-terminal half of SmIIIA confer affinity for tetrodotoxin-resistant sodium channels in the cell bodies of frog sympathetic neurons. SmIIIA and the chimera possessing the C-terminal half of SmIIIA also inhibit tetrodotoxin-resistant sodium channels in the postganglionic axons of sympathetic neurons, as indicated by their inhibition of C-neuron compound action potentials that persist in the presence of tetrodotoxin.

-	~~{{STRUCTURE_1q2j\| PDB=1q2j \| SCENE= }}~~	+	Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA.,Keizer DW, West PJ, Lee EF, Yoshikami D, Olivera BM, Bulaj G, Norton RS J Biol Chem. 2003 Nov 21;278(47):46805-13. Epub 2003 Sep 10. PMID:12970353<ref>PMID:12970353</ref>

-	~~===Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12970353}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1q2j]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA].~~	+	</StructureSection>
	[[Category: Bulaj, G.]]		[[Category: Bulaj, G.]]
	[[Category: Keizer, D W.]]		[[Category: Keizer, D W.]]

OCA: Protected "1q2j" [edit=sysop:move=sysop]

2013-01-02T09:47:21Z

Protected "1q2j" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:47, 2 January 2013

OCA at 09:47, 2 January 2013

2013-01-02T09:47:19Z

←Older revision		Revision as of 09:47, 2 January 2013
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	[[Image:1q2j.png\|left\|200px]]		[[Image:1q2j.png\|left\|200px]]

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	===Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA===		===Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA===

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	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA].	+	[[1q2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2J OCA].
-		+
-	~~==Reference==~~	+
-	Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA., Keizer DW, West PJ, Lee EF, Yoshikami D, Olivera BM, Bulaj G, Norton RS, J Biol Chem. 2003 Nov 21;278(47):46805-13. Epub 2003 Sep 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970353 12970353]	+
	[[Category: Bulaj, G.]]		[[Category: Bulaj, G.]]
	[[Category: Keizer, D W.]]		[[Category: Keizer, D W.]]
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	[[Category: Yoshikami, D.]]		[[Category: Yoshikami, D.]]
	[[Category: Mu-conotoxin]]		[[Category: Mu-conotoxin]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Jul 29 08:28:32 2008''~~	+