1qxm - Revision history

OCA at 08:18, 14 February 2024

2024-02-14T08:18:35Z

←Older revision		Revision as of 08:18, 14 February 2024
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	<StructureSection load='1qxm' size='340' side='right'caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1qxm' size='340' side='right'caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_c3 Bacteriophage c3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_D_phage Clostridium botulinum D phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [https://pdbe.org/1qxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [https://www.ebi.ac.uk/pdbsum/1qxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [https://pdbe.org/1qxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [https://www.ebi.ac.uk/pdbsum/1qxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~HA33_CLOBO HA33_CLOBO~~]~~] Protects~~ the structural integrity of the neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. Involved in binding to the small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties.	+	[https://www.uniprot.org/uniprot/HA33D_CBDP HA33D_CBDP] The hemagglutinin (HA) component of the progenitor toxin protects the structural integrity of the neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. Involved in binding to the small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties (By similarity). Erythrocyte agglutination only occurs when the entire complex is assembled (PubMed:17581814). Binds eukaryotic host mucins as well as N-acetyl-beta-neuraminic acid, N-acetyl-D-galactosamine and galactose (but not glucose) via 2 sites (By similarity).[UniProtKB:P0DPR0]<ref>PMID:17581814</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxm ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Botulism food poisoning is caused primarily by ingestion of the Clostridium botulinum neurotoxin (BoNT). The 1300 amino acid BoNT forms a progenitor toxin (PTX) that, when associated with a number of other proteins, increases its oral toxicity by protecting it from the low pH of the stomach and from intestinal proteases. One of these associated proteins, HA1, has also been suggested to be involved with internalization of the toxin into the bloodstream by binding to oligosaccharides lining the intestine. Here is reported the crystal structure of HA1 from type C Clostridium botulinum at a resolution of 1.7 Angstrom. The protein consists of two beta-trefoil domains and bears structural similarities to the lectin B-chain from the deadly plant toxin ricin. Based on structural comparison to the ricin B-chain lactose-binding sites, residues of type A HA1 were selected and mutated. The D263A and N285A mutants lost the ability to bind carbohydrates containing galactose moieties, implicating these residues in carbohydrate binding.
-
-	Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum.,Inoue K, Sobhany M, Transue TR, Oguma K, Pedersen LC, Negishi M Microbiology. 2003 Dec;149(Pt 12):3361-70. PMID:14663070<ref>PMID:14663070</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qxm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage c3~~]]	+	[[Category: Clostridium botulinum D phage]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Inoue, K]]	+	[[Category: Inoue K]]
-	[[Category: Negishi, M]]	+	[[Category: Negishi M]]
-	[[Category: Oguma, K]]	+	[[Category: Oguma K]]
-	[[Category: Pedersen~~, L C~~]]	+	[[Category: Pedersen LC]]
-	[[Category: Sobhany, M]]	+	[[Category: Sobhany M]]
-	[[Category: Transue~~, T R]]~~	+	[[Category: Transue TR]]
-	~~[[Category: Clostridium botulinum]]~~	+
-	~~[[Category: Ha1]]~~	+
-	~~[[Category: Hemagglutinin]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Trefoil~~]]	+

OCA at 09:00, 21 April 2021

2021-04-21T09:00:16Z

←Older revision		Revision as of 09:00, 21 April 2021
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	<StructureSection load='1qxm' size='340' side='right'caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1qxm' size='340' side='right'caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QXM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [~~http~~://pdbe.org/1qxm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qxm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [https://pdbe.org/1qxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [https://www.ebi.ac.uk/pdbsum/1qxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HA33_CLOBO HA33_CLOBO]] Protects the structural integrity of the neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. Involved in binding to the small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties.	+	[[https://www.uniprot.org/uniprot/HA33_CLOBO HA33_CLOBO]] Protects the structural integrity of the neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. Involved in binding to the small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Hemagglutinin\|Hemagglutinin]]	+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:00, 17 July 2019

2019-07-17T13:00:10Z

←Older revision		Revision as of 13:00, 17 July 2019
Line 1:		Line 1:

	==Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum==		==Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum==
-	<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1qxm' size='340' side='right'caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxm_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxm_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage c3]]		[[Category: Bacteriophage c3]]
		+	[[Category: Large Structures]]
	[[Category: Inoue, K]]		[[Category: Inoue, K]]
	[[Category: Negishi, M]]		[[Category: Negishi, M]]

OCA at 12:30, 12 October 2017

2017-10-12T12:30:43Z

←Older revision		Revision as of 12:30, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum==		==Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum==
	<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://pdbe.org/1qxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://pdbe.org/1qxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:31, 8 February 2016

2016-02-08T05:31:01Z

←Older revision		Revision as of 05:31, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:22, 10 September 2015

2015-09-10T08:22:44Z

←Older revision		Revision as of 08:22, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum_d_phage Clostridium botulinum d phage~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_c3 Bacteriophage c3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://pdbe.org/1qxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qxm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium botulinum d phage~~]]	+	[[Category: Bacteriophage c3]]
	[[Category: Inoue, K]]		[[Category: Inoue, K]]
	[[Category: Negishi, M]]		[[Category: Negishi, M]]

OCA at 20:58, 24 December 2014

2014-12-24T20:58:50Z

←Older revision		Revision as of 20:58, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HA33_CLOBO HA33_CLOBO]] Protects the structural integrity of the neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. Involved in binding to the small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium botulinum d phage]]		[[Category: Clostridium botulinum d phage]]
-	[[Category: Inoue, K.]]	+	[[Category: Inoue, K]]
-	[[Category: Negishi, M.]]	+	[[Category: Negishi, M]]
-	[[Category: Oguma, K.]]	+	[[Category: Oguma, K]]
-	[[Category: Pedersen, L C.]]	+	[[Category: Pedersen, L C]]
-	[[Category: Sobhany, M.]]	+	[[Category: Sobhany, M]]
-	[[Category: Transue, T R.]]	+	[[Category: Transue, T R]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Ha1]]		[[Category: Ha1]]

OCA at 16:49, 29 September 2014

2014-09-29T16:49:13Z

←Older revision		Revision as of 16:49, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1qxm\| PDB=1qxm \| SCENE= }}~~	+	==Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum==
-	===Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum===	+	<StructureSection load='1qxm' size='340' side='right' caption='[[1qxm]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_14663070}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QXM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qxm RCSB], [http://www.ebi.ac.uk/pdbsum/1qxm PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxm_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulism food poisoning is caused primarily by ingestion of the Clostridium botulinum neurotoxin (BoNT). The 1300 amino acid BoNT forms a progenitor toxin (PTX) that, when associated with a number of other proteins, increases its oral toxicity by protecting it from the low pH of the stomach and from intestinal proteases. One of these associated proteins, HA1, has also been suggested to be involved with internalization of the toxin into the bloodstream by binding to oligosaccharides lining the intestine. Here is reported the crystal structure of HA1 from type C Clostridium botulinum at a resolution of 1.7 Angstrom. The protein consists of two beta-trefoil domains and bears structural similarities to the lectin B-chain from the deadly plant toxin ricin. Based on structural comparison to the ricin B-chain lactose-binding sites, residues of type A HA1 were selected and mutated. The D263A and N285A mutants lost the ability to bind carbohydrates containing galactose moieties, implicating these residues in carbohydrate binding.

-	~~==About this Structure==~~	+	Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum.,Inoue K, Sobhany M, Transue TR, Oguma K, Pedersen LC, Negishi M Microbiology. 2003 Dec;149(Pt 12):3361-70. PMID:14663070<ref>PMID:14663070</ref>
-	~~[[1qxm]] is~~ a ~~2 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage~~ Clostridium botulinum ~~d phage]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1QXM OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Hemagglutinin\|Hemagglutinin]]		*[[Hemagglutinin\|Hemagglutinin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:014663070<~~/~~ref~~><~~ref group="xtra">PMID:017009862</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium botulinum d phage]]		[[Category: Clostridium botulinum d phage]]
	[[Category: Inoue, K.]]		[[Category: Inoue, K.]]

OCA at 10:16, 11 March 2013

2013-03-11T10:16:12Z

←Older revision		Revision as of 10:16, 11 March 2013
Line 1:		Line 1:
-	[[Image:1qxm.png\|left\|200px]]
-
	{{STRUCTURE_1qxm\| PDB=1qxm \| SCENE= }}		{{STRUCTURE_1qxm\| PDB=1qxm \| SCENE= }}
-
	===Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum===		===Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum===
-
	{{ABSTRACT_PUBMED_14663070}}		{{ABSTRACT_PUBMED_14663070}}

OCA at 09:04, 20 October 2012

2012-10-20T09:04:33Z

←Older revision		Revision as of 09:04, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1qxm]] is a 2 chain structure ~~of [[Hemagglutinin]]~~ with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA].	+	[[1qxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA].

	==See Also==		==See Also==