1r2u - Revision history

OCA at 09:02, 22 May 2024

2024-05-22T09:02:55Z

←Older revision		Revision as of 09:02, 22 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2u ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Adaptation for life at different temperatures can cause changes in many aspects of an organism. One example is the expression of different protein isoforms in species adapted to different temperatures. The calcium regulatory protein cardiac troponin C (cTnC), from rainbow trout (Oncorhynchus mykiss), is a good model for studying temperature effects, both because of its low physiological temperature and because mammalian cTnC, extensively studied at higher temperatures, can be used for comparison. We determined the structure and studied the backbone dynamics of the regulatory domain of trout cardiac troponin C (ScNTnC) with one Ca(2+) bound at 7 and 30 degrees C, using nuclear magnetic resonance spectroscopy (NMR). The overall fold of the regulatory domain of trout cTnC at both temperatures is similar to the regulatory domain of mammalian (human, bovine, and porcine isoform) cTnC bound to one Ca(2+). By comparing the trout structures at the two temperatures, we identify differences between the positions of the helices flanking the calcium binding loops, and the overall structure at 7 degrees C is more compact than that at 30 degrees C. The structure at 7 degrees C is more similar to the mammalian cTnC, which was determined at 30 degrees C, indicating that they have the same conformation at their respective physiological temperatures. The dynamic properties of the regulatory domain of trout cTnC are similar at the two temperatures that were used in these studies.
		+
		+	Effect of temperature on the structure of trout troponin C.,Blumenschein TM, Gillis TE, Tibbits GF, Sykes BD Biochemistry. 2004 May 4;43(17):4955-63. PMID:15109253<ref>PMID:15109253</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1r2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Troponin 3D structures\|Troponin 3D structures]]		*[[Troponin 3D structures\|Troponin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:09, 17 April 2024

2024-04-17T06:09:00Z

←Older revision		Revision as of 06:09, 17 April 2024
Line 1:		Line 1:

	==NMR structure of the N domain of trout cardiac troponin C at 30 C==		==NMR structure of the N domain of trout cardiac troponin C at 30 C==
-	<StructureSection load='1r2u' size='340' side='right'caption='[[1r2u~~]], [[NMR_Ensembles_of_Models \| 40 NMR models~~]]' scene=''>	+	<StructureSection load='1r2u' size='340' side='right'caption='[[1r2u]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Oncmy Oncmy~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [https://pdbe.org/1r2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [https://www.ebi.ac.uk/pdbsum/1r2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [https://pdbe.org/1r2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [https://www.ebi.ac.uk/pdbsum/1r2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q7ZZB9_ONCMY Q7ZZB9_ONCMY]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2u ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Adaptation for life at different temperatures can cause changes in many aspects of an organism. One example is the expression of different protein isoforms in species adapted to different temperatures. The calcium regulatory protein cardiac troponin C (cTnC), from rainbow trout (Oncorhynchus mykiss), is a good model for studying temperature effects, both because of its low physiological temperature and because mammalian cTnC, extensively studied at higher temperatures, can be used for comparison. We determined the structure and studied the backbone dynamics of the regulatory domain of trout cardiac troponin C (ScNTnC) with one Ca(2+) bound at 7 and 30 degrees C, using nuclear magnetic resonance spectroscopy (NMR). The overall fold of the regulatory domain of trout cTnC at both temperatures is similar to the regulatory domain of mammalian (human, bovine, and porcine isoform) cTnC bound to one Ca(2+). By comparing the trout structures at the two temperatures, we identify differences between the positions of the helices flanking the calcium binding loops, and the overall structure at 7 degrees C is more compact than that at 30 degrees C. The structure at 7 degrees C is more similar to the mammalian cTnC, which was determined at 30 degrees C, indicating that they have the same conformation at their respective physiological temperatures. The dynamic properties of the regulatory domain of trout cTnC are similar at the two temperatures that were used in these studies.
-
-	Effect of temperature on the structure of trout troponin C.,Blumenschein TM, Gillis TE, Tibbits GF, Sykes BD Biochemistry. 2004 May 4;43(17):4955-63. PMID:15109253<ref>PMID:15109253</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1r2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Troponin\|Troponin]]	+	*[[Troponin 3D structures\|Troponin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Oncmy~~]]	+	[[Category: Oncorhynchus mykiss]]
-	[[Category: Blumenschein~~, T M~~]]	+	[[Category: Blumenschein TM]]
-	[[Category: Gillis~~, T E~~]]	+	[[Category: Gillis TE]]
-	[[Category: Sykes~~, B D~~]]	+	[[Category: Sykes BD]]
-	[[Category: Tibbits~~, G F]]~~	+	[[Category: Tibbits GF]]
-	~~[[Category: Ef-hand]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 10:07, 15 September 2021

2021-09-15T10:07:42Z

←Older revision		Revision as of 10:07, 15 September 2021
Line 3:		Line 3:
	<StructureSection load='1r2u' size='340' side='right'caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='1r2u' size='340' side='right'caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1R2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [~~http~~://pdbe.org/1r2u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1r2u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [https://pdbe.org/1r2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [https://www.ebi.ac.uk/pdbsum/1r2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2u ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:45, 4 December 2019

2019-12-04T11:45:09Z

←Older revision		Revision as of 11:45, 4 December 2019
Line 1:		Line 1:

	==NMR structure of the N domain of trout cardiac troponin C at 30 C==		==NMR structure of the N domain of trout cardiac troponin C at 30 C==
-	<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>	+	<StructureSection load='1r2u' size='340' side='right'caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Oncmy]]		[[Category: Oncmy]]
	[[Category: Blumenschein, T M]]		[[Category: Blumenschein, T M]]

OCA at 08:09, 28 February 2018

2018-02-28T08:09:14Z

←Older revision		Revision as of 08:09, 28 February 2018
Line 1:		Line 1:
		+
	==NMR structure of the N domain of trout cardiac troponin C at 30 C==		==NMR structure of the N domain of trout cardiac troponin C at 30 C==
	<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://pdbe.org/1r2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://pdbe.org/1r2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2u ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r2u_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r2u_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:40, 11 September 2015

2015-09-11T11:40:57Z

←Older revision		Revision as of 11:40, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Oncorhynchus_mykiss Oncorhynchus mykiss~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncmy Oncmy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://pdbe.org/1r2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1r2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Oncorhynchus mykiss~~]]	+	[[Category: Oncmy]]
	[[Category: Blumenschein, T M]]		[[Category: Blumenschein, T M]]
	[[Category: Gillis, T E]]		[[Category: Gillis, T E]]

OCA at 08:23, 6 January 2015

2015-01-06T08:23:16Z

←Older revision		Revision as of 08:23, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Oncorhynchus mykiss]]		[[Category: Oncorhynchus mykiss]]
-	[[Category: Blumenschein, T M.]]	+	[[Category: Blumenschein, T M]]
-	[[Category: Gillis, T E.]]	+	[[Category: Gillis, T E]]
-	[[Category: Sykes, B D.]]	+	[[Category: Sykes, B D]]
-	[[Category: Tibbits, G F.]]	+	[[Category: Tibbits, G F]]
	[[Category: Ef-hand]]		[[Category: Ef-hand]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]

OCA at 20:36, 28 September 2014

2014-09-28T20:36:20Z

←Older revision		Revision as of 20:36, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1r2u.~~png\|left~~\|200px]]	+	==NMR structure of the N domain of trout cardiac troponin C at 30 C==
		+	<StructureSection load='1r2u' size='340' side='right' caption='[[1r2u]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R2U FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r2u RCSB], [http://www.ebi.ac.uk/pdbsum/1r2u PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r2u_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Adaptation for life at different temperatures can cause changes in many aspects of an organism. One example is the expression of different protein isoforms in species adapted to different temperatures. The calcium regulatory protein cardiac troponin C (cTnC), from rainbow trout (Oncorhynchus mykiss), is a good model for studying temperature effects, both because of its low physiological temperature and because mammalian cTnC, extensively studied at higher temperatures, can be used for comparison. We determined the structure and studied the backbone dynamics of the regulatory domain of trout cardiac troponin C (ScNTnC) with one Ca(2+) bound at 7 and 30 degrees C, using nuclear magnetic resonance spectroscopy (NMR). The overall fold of the regulatory domain of trout cTnC at both temperatures is similar to the regulatory domain of mammalian (human, bovine, and porcine isoform) cTnC bound to one Ca(2+). By comparing the trout structures at the two temperatures, we identify differences between the positions of the helices flanking the calcium binding loops, and the overall structure at 7 degrees C is more compact than that at 30 degrees C. The structure at 7 degrees C is more similar to the mammalian cTnC, which was determined at 30 degrees C, indicating that they have the same conformation at their respective physiological temperatures. The dynamic properties of the regulatory domain of trout cTnC are similar at the two temperatures that were used in these studies.

-	~~{{STRUCTURE_1r2u\| PDB=1r2u \| SCENE= }}~~	+	Effect of temperature on the structure of trout troponin C.,Blumenschein TM, Gillis TE, Tibbits GF, Sykes BD Biochemistry. 2004 May 4;43(17):4955-63. PMID:15109253<ref>PMID:15109253</ref>

-	~~===NMR structure~~ of the ~~N domain of trout cardiac troponin C at 30 C===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15109253}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1r2u]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA].~~	+

	==See Also==		==See Also==
	*[[Troponin\|Troponin]]		*[[Troponin\|Troponin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015109253<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Oncorhynchus mykiss]]		[[Category: Oncorhynchus mykiss]]
	[[Category: Blumenschein, T M.]]		[[Category: Blumenschein, T M.]]

OCA at 18:37, 20 October 2012

2012-10-20T18:37:38Z

←Older revision		Revision as of 18:37, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1r2u]] is a 1 chain structure ~~of [[Troponin]]~~ with sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA].	+	[[1r2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2U OCA].

	==See Also==		==See Also==

OCA at 04:41, 27 July 2012

2012-07-27T04:41:05Z

←Older revision		Revision as of 04:41, 27 July 2012
Line 1:		Line 1:
	[[Image:1r2u.png\|left\|200px]]		[[Image:1r2u.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1r2u", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1r2u\| PDB=1r2u \| SCENE= }}		{{STRUCTURE_1r2u\| PDB=1r2u \| SCENE= }}

	===NMR structure of the N domain of trout cardiac troponin C at 30 C===		===NMR structure of the N domain of trout cardiac troponin C at 30 C===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15109253}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15109253 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15109253}}		{{ABSTRACT_PUBMED_15109253}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Troponin]]	+	*[[Troponin\|Troponin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15109253~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015109253</ref><references group="xtra"/>
	[[Category: Oncorhynchus mykiss]]		[[Category: Oncorhynchus mykiss]]
	[[Category: Blumenschein, T M.]]		[[Category: Blumenschein, T M.]]