1r5r - Revision history

OCA: Redirecting to 3bjh

2008-03-23T14:18:56Z

Redirecting to 3bjh

←Older revision		Revision as of 14:18, 23 March 2008
Line 1:		Line 1:
-	[[~~Image:1r5r.jpg\|left\|200px~~]]	+	#REDIRECT [[3bjh]] This PDB entry is obsolete and replaced by 3bjh
-		+
-	~~{{Structure~~	+
-	\|PDB~~= 1r5r \|SIZE=350\|CAPTION= <scene name='initialview01'>1r5r</scene>~~	+
-	~~\|SITE=~~	+
-	~~\|LIGAND=~~	+
-	~~\|ACTIVITY=~~	+
-	~~\|GENE=~~	+
-	}}	+
-		+
-	~~''''''~~	+
-		+
-		+
-	~~==About this Structure==~~	+
-	is ~~a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].~~	+
-	~~[[Category: Protein complex]]~~	+
-		+
-	~~''Page seeded~~ by ~~[http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:25:59 2008''~~	+

OCA at 11:26, 23 March 2008

2008-03-23T11:26:01Z

←Older revision		Revision as of 11:26, 23 March 2008
Line 1:		Line 1:
-	~~#REDIRECT~~ [[~~3bjh~~]] ~~This~~ PDB ~~entry~~ is ~~obsolete and replaced~~ by ~~3bjh~~	+	[[Image:1r5r.jpg\|left\|200px]]
		+
		+	{{Structure
		+	\|PDB= 1r5r \|SIZE=350\|CAPTION= <scene name='initialview01'>1r5r</scene>
		+	\|SITE=
		+	\|LIGAND=
		+	\|ACTIVITY=
		+	\|GENE=
		+	}}
		+
		+	''''''
		+
		+
		+	==About this Structure==
		+	is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA].
		+	[[Category: Protein complex]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:25:59 2008''

OCA: Redirecting to 3bjh

2008-03-19T11:56:12Z

Redirecting to 3bjh

←Older revision		Revision as of 11:56, 19 March 2008
Line 1:		Line 1:
-	[[~~Image:1r5r.jpg\|left\|200px~~]]~~<br /><applet load="1r5r" size="450" color="white" frame="true" align="right" spinBox="true"~~	+	#REDIRECT [[3bjh]] This PDB entry is obsolete and replaced by 3bjh
-	~~caption="1r5r, resolution 1.60Å" />~~	+
-	~~'''Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.'''<br />~~	+
-		+
-	~~==Overview==~~	+
-	Pheromone binding proteins (PBPs) are small helical proteins (, approximately 13-17 kDa) present in several sensory organs from moth and, other insect species. They are involved in the transport of pheromones, from the sensillar lymph to the olfactory receptors. We report here the, crystal structure of a PBP (Amel-ASP1) originating from the honey-bee, (Apis mellifera) antennae and expressed as recombinant protein in the, yeast Pichia pastoris. Crystals of Amel-ASP1 were obtained at pH 5.5 using, the nano-drops technique of crystallization with a novel optimization, procedure, and the structure was solved initially with the, single-wavelength anomalous diffraction technique using sulfur anomalous, dispersion. The structure of Amel-ASP1 has been refined at 1.6-A, resolution. Its fold is ~~roughly similar to that of other PBP/odorant, binding proteins, presenting six helices~~ and three disulfide bridges., Contrary to the PBPs from Bombyx mori (Sandler, B. H., Nikonova, L., Leal, W. S., and Clardy, J. (2000) Chem. Biol. 7, 143-151) and Leucophea maderae, (Lartigue, A., Gruez, A., Spinelli, S., Riviere, S., Brossut, R., Tegoni, M., and Cambillau, C. (2003) J. Biol. Chem. 278, 30213-30218), the, extended C terminus folds into the protein and forms a wall of the, internal hydrophobic cavity. Its backbone groups establish two hydrogen, bonds with a serendipitous ligand, n-butyl-benzene-sulfonamide, an, additive used in plastics. This mode of binding might, however, mimic that, used by ~~one of the pheromonal blend components and illustrates the binding, versatility of PBPs.~~	+
-		+
-	~~==About this Structure==~~	+
-	1R5R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera] with NBB as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R5R OCA].	+
-		+
-	~~==Reference==~~	+
-	Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14594955 14594955]	+
-	~~[[Category: Apis mellifera]]~~	+
-	~~[[Category: Single protein]]~~	+
-	~~[[Category: Bezirard, V.]]~~	+
-	~~[[Category: Blon, F.]]~~	+
-	~~[[Category: Briand, L.]]~~	+
-	~~[[Category: Cambillau, C.]]~~	+
-	~~[[Category: Gruez, A.]]~~	+
-	~~[[Category: Lartigue, A.]]~~	+
-	~~[[Category: Pernollet, J.C.]]~~	+
-	~~[[Category: Tegoni, M.]]~~	+
-	~~[[Category: Walsh, M.]]~~	+
-	~~[[Category: NBB]]~~	+
-	~~[[Category: pheromone binding protein (pbp)]]~~	+
-	~~[[Category: s-sad]]~~	+
-		+
-	~~''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:11:44 2007''~~	+

OCA: New page: 200px
'''Soft-SAD crystal str...
2007-11-24T20:04:14Z

New page: 200px<br /><applet load="1r5r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r5r, resolution 1.60Å" /> '''Soft-SAD crystal str...

New page
[[Image:1r5r.jpg|left|200px]]<br /><applet load="1r5r" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1r5r, resolution 1.60Å" />
'''Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.'''<br />

==Overview==
Pheromone binding proteins (PBPs) are small helical proteins (, approximately 13-17 kDa) present in several sensory organs from moth and, other insect species. They are involved in the transport of pheromones, from the sensillar lymph to the olfactory receptors. We report here the, crystal structure of a PBP (Amel-ASP1) originating from the honey-bee, (Apis mellifera) antennae and expressed as recombinant protein in the, yeast Pichia pastoris. Crystals of Amel-ASP1 were obtained at pH 5.5 using, the nano-drops technique of crystallization with a novel optimization, procedure, and the structure was solved initially with the, single-wavelength anomalous diffraction technique using sulfur anomalous, dispersion. The structure of Amel-ASP1 has been refined at 1.6-A, resolution. Its fold is roughly similar to that of other PBP/odorant, binding proteins, presenting six helices and three disulfide bridges., Contrary to the PBPs from Bombyx mori (Sandler, B. H., Nikonova, L., Leal, W. S., and Clardy, J. (2000) Chem. Biol. 7, 143-151) and Leucophea maderae, (Lartigue, A., Gruez, A., Spinelli, S., Riviere, S., Brossut, R., Tegoni, M., and Cambillau, C. (2003) J. Biol. Chem. 278, 30213-30218), the, extended C terminus folds into the protein and forms a wall of the, internal hydrophobic cavity. Its backbone groups establish two hydrogen, bonds with a serendipitous ligand, n-butyl-benzene-sulfonamide, an, additive used in plastics. This mode of binding might, however, mimic that, used by one of the pheromonal blend components and illustrates the binding, versatility of PBPs.

==About this Structure==
1R5R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera] with NBB as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R5R OCA].

==Reference==
Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14594955 14594955]
[[Category: Apis mellifera]]
[[Category: Single protein]]
[[Category: Bezirard, V.]]
[[Category: Blon, F.]]
[[Category: Briand, L.]]
[[Category: Cambillau, C.]]
[[Category: Gruez, A.]]
[[Category: Lartigue, A.]]
[[Category: Pernollet, J.C.]]
[[Category: Tegoni, M.]]
[[Category: Walsh, M.]]
[[Category: NBB]]
[[Category: pheromone binding protein (pbp)]]
[[Category: s-sad]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:11:44 2007''