1rst - Revision history

OCA at 08:25, 14 February 2024

2024-02-14T08:25:43Z

←Older revision		Revision as of 08:25, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1rst' size='340' side='right'caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1rst' size='340' side='right'caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RST FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RST FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [https://pdbe.org/1rst PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [https://www.ebi.ac.uk/pdbsum/1rst PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rst ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [https://pdbe.org/1rst PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [https://www.ebi.ac.uk/pdbsum/1rst PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rst ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rst ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rst ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Strep-tag is a selected nine-amino acid peptide (AWRHPQFGG) that displays intrinsic binding affinity towards streptavidin and has been used as an affinity tag for recombinant proteins. In order to elucidate the molecular mechanism underlying this type of artificial protein-peptide recognition, X-ray crystallographic analyses and binding measurements were carried out. The crystal structure of the complex between recombinant core streptavidin and the synthesized peptide was solved and refined at 1.7 A resolution (space group I4(1)22; unit cell dimensions a = b = 58.3 A, c = 176.9 A). The Strep-tag was bound at the same surface pocket where biotin, the natural ligand of streptavidin, gets complexed. The peptide backbone exhibited 3(10)-helical conformation, with eight of the residues involved in protein contacts. The C-terminal Gly-Gly moiety of the Strep-tag participated in a salt bridge to Arg84 of streptavidin with its free carboxylate group. This finding explained why the use of the Strep-tag in fusions with recombinant proteins was restricted to their carboxyl end. Employing a synthetic peptide spot assay, the variant Strep-tag II was screened, which did not have this limitation. The isomorphous crystal structure of its complex with streptavidin revealed that a glutamate side-chain provided the salt bridge in this case, with an otherwise almost unchanged mode of binding. Affinity constants between the peptides and streptavidin were measured by isothermal titration calorimetry. A value of 2.7 x 10(4) M-1 was determined for the Strep-tag peptide, and slightly tighter binding was seen when the Strep-tag was applied as part of a bacterially produced fusion protein. This affinity is significantly higher, compared with values previously reported for shorter streptavidin-binding peptides, and agrees well with the remarkable selectivity observed in recombinant protein purification applications.
-
-	Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.,Schmidt TG, Koepke J, Frank R, Skerra A J Mol Biol. 1996 Feb 9;255(5):753-66. PMID:8636976<ref>PMID:8636976</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1rst" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Koepke, J~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Schmidt, T~~]]	+	[[Category: Koepke J]]
-	[[Category: ~~Skerra, A~~]]	+	[[Category: Schmidt T]]
-	[[Category: ~~Signal protein~~]]	+	[[Category: Skerra A]]

OCA at 07:21, 22 September 2021

2021-09-22T07:21:08Z

←Older revision		Revision as of 07:21, 22 September 2021
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	<StructureSection load='1rst' size='340' side='right'caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1rst' size='340' side='right'caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RST FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RST FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [~~http~~://pdbe.org/1rst PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1rst PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1rst ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [https://pdbe.org/1rst PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [https://www.ebi.ac.uk/pdbsum/1rst PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rst ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:46, 11 December 2019

2019-12-11T19:46:44Z

←Older revision		Revision as of 19:46, 11 December 2019
Line 1:		Line 1:

	==COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE==		==COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE==
-	<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1rst' size='340' side='right'caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Avidin\|Avidin]]	+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Koepke, J]]		[[Category: Koepke, J]]
	[[Category: Schmidt, T]]		[[Category: Schmidt, T]]
	[[Category: Skerra, A]]		[[Category: Skerra, A]]
	[[Category: Signal protein]]		[[Category: Signal protein]]

OCA at 07:17, 8 March 2018

2018-03-08T07:17:20Z

←Older revision		Revision as of 07:17, 8 March 2018
Line 1:		Line 1:
		+
	==COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE==		==COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE==
	<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://pdbe.org/1rst PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://pdbe.org/1rst PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rst ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/1rst_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/1rst_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:00, 8 February 2016

2016-02-08T21:00:37Z

←Older revision		Revision as of 21:00, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rst ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:20, 10 September 2015

2015-09-10T15:20:42Z

←Older revision		Revision as of 15:20, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://pdbe.org/1rst PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1rst" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Koepke, J]]		[[Category: Koepke, J]]
	[[Category: Schmidt, T]]		[[Category: Schmidt, T]]
	[[Category: Skerra, A]]		[[Category: Skerra, A]]
	[[Category: Signal protein]]		[[Category: Signal protein]]

OCA at 02:06, 25 December 2014

2014-12-25T02:06:54Z

←Older revision		Revision as of 02:06, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Koepke, J.]]	+	[[Category: Koepke, J]]
-	[[Category: Schmidt, T.]]	+	[[Category: Schmidt, T]]
-	[[Category: Skerra, A.]]	+	[[Category: Skerra, A]]
	[[Category: Signal protein]]		[[Category: Signal protein]]

OCA at 23:38, 28 September 2014

2014-09-28T23:38:56Z

←Older revision		Revision as of 23:38, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1rst.~~png\|left~~\|200px]]	+	==COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE==
		+	<StructureSection load='1rst' size='340' side='right' caption='[[1rst]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RST FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rst FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rst OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rst RCSB], [http://www.ebi.ac.uk/pdbsum/1rst PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/1rst_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Strep-tag is a selected nine-amino acid peptide (AWRHPQFGG) that displays intrinsic binding affinity towards streptavidin and has been used as an affinity tag for recombinant proteins. In order to elucidate the molecular mechanism underlying this type of artificial protein-peptide recognition, X-ray crystallographic analyses and binding measurements were carried out. The crystal structure of the complex between recombinant core streptavidin and the synthesized peptide was solved and refined at 1.7 A resolution (space group I4(1)22; unit cell dimensions a = b = 58.3 A, c = 176.9 A). The Strep-tag was bound at the same surface pocket where biotin, the natural ligand of streptavidin, gets complexed. The peptide backbone exhibited 3(10)-helical conformation, with eight of the residues involved in protein contacts. The C-terminal Gly-Gly moiety of the Strep-tag participated in a salt bridge to Arg84 of streptavidin with its free carboxylate group. This finding explained why the use of the Strep-tag in fusions with recombinant proteins was restricted to their carboxyl end. Employing a synthetic peptide spot assay, the variant Strep-tag II was screened, which did not have this limitation. The isomorphous crystal structure of its complex with streptavidin revealed that a glutamate side-chain provided the salt bridge in this case, with an otherwise almost unchanged mode of binding. Affinity constants between the peptides and streptavidin were measured by isothermal titration calorimetry. A value of 2.7 x 10(4) M-1 was determined for the Strep-tag peptide, and slightly tighter binding was seen when the Strep-tag was applied as part of a bacterially produced fusion protein. This affinity is significantly higher, compared with values previously reported for shorter streptavidin-binding peptides, and agrees well with the remarkable selectivity observed in recombinant protein purification applications.

-	~~{{STRUCTURE_1rst\| PDB=1rst \| SCENE= }}~~	+	Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.,Schmidt TG, Koepke J, Frank R, Skerra A J Mol Biol. 1996 Feb 9;255(5):753-66. PMID:8636976<ref>PMID:8636976</ref>

-	~~===COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8636976}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1rst]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_avidinii Streptomyces avidinii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008636976<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Koepke, J.]]		[[Category: Koepke, J.]]

OCA at 11:27, 20 October 2012

2012-10-20T11:27:51Z

←Older revision		Revision as of 11:27, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1rst]] is a 2 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA].	+	[[1rst]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RST OCA].

	==See Also==		==See Also==

OCA at 05:28, 26 July 2012

2012-07-26T05:28:07Z

←Older revision		Revision as of 05:28, 26 July 2012
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	[[Image:1rst.png\|left\|200px]]		[[Image:1rst.png\|left\|200px]]

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	{{STRUCTURE_1rst\| PDB=1rst \| SCENE= }}		{{STRUCTURE_1rst\| PDB=1rst \| SCENE= }}

	===COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE===		===COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE===

-
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	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==