1ru3 - Revision history

OCA at 08:27, 1 May 2024

2024-05-01T08:27:06Z

←Older revision		Revision as of 08:27, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [https://pdbe.org/1ru3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [https://pdbe.org/1ru3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/P83789_CARHY P83789_CARHY]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ru3 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ru3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In anaerobic microorganisms employing the acetyl-CoA pathway, acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH) form a complex (ACS/CODH) that catalyzes the synthesis of acetyl-CoA from CO, a methyl group, and CoA. Previously, a [4Fe-4S] cubane bridged to a copper-nickel binuclear site (active site cluster A of the ACS component) was identified in the ACS(Mt)/CODH(Mt) from Moorella thermoacetica whereas another study revealed a nickel-nickel site in the open form of ACS(Mt), and a zink-nickel site in the closed form. The ACS(Ch) of the hydrogenogenic bacterium Carboxydothermus hydrogenoformans was found to exist as an 82.2-kDa monomer as well as in a 1:1 molar complex with the 73.3-kDa CODHIII(Ch). Homogeneous ACS(Ch) and ACS(Ch)/CODHIII(Ch) catalyzed the exchange between [1-(14)C]acetyl-CoA and (12)CO with specific activities of 2.4 or 5.9 micromol of CO per min per mg, respectively, at 70 degrees C and pH 6.0. They also catalyzed the synthesis of acetyl-CoA from CO, methylcobalamin, corrinoid iron-sulfur protein, and CoA with specific activities of 0.14 or 0.91 micromol of acetyl-CoA formed per min per mg, respectively, at 70 degrees C and pH 7.3. The functional cluster A of ACS(Ch) contains a Ni-Ni-[4Fe-4S] site, in which the positions proximal and distal to the cubane are occupied by Ni ions. This result is apparent from a positive correlation of the Ni contents and negative correlations of the Cu or Zn contents with the acetyl-CoA/CO exchange activities of different preparations of monomeric ACS(Ch), a 2.2-A crystal structure of the dithionite-reduced monomer in an open conformation, and x-ray absorption spectroscopy.
-
-	A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans.,Svetlitchnyi V, Dobbek H, Meyer-Klaucke W, Meins T, Thiele B, Romer P, Huber R, Meyer O Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):446-51. Epub 2003 Dec 29. PMID:14699043<ref>PMID:14699043</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ru3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 25:
	*[[Acetyl-CoA synthase 3D structures\|Acetyl-CoA synthase 3D structures]]		*[[Acetyl-CoA synthase 3D structures\|Acetyl-CoA synthase 3D structures]]
	*[[IronÃ¢ÂÂsulfur proteins\|IronÃ¢ÂÂsulfur proteins]]		*[[IronÃ¢ÂÂsulfur proteins\|IronÃ¢ÂÂsulfur proteins]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Carboxydothermus hydrogenoformans]]		[[Category: Carboxydothermus hydrogenoformans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dobbek, H]]	+	[[Category: Dobbek H]]
-	[[Category: Huber, R]]	+	[[Category: Huber R]]
-	[[Category: Meins, T]]	+	[[Category: Meins T]]
-	[[Category: Meyer, O]]	+	[[Category: Meyer O]]
-	[[Category: Meyer-Klaucke, W]]	+	[[Category: Meyer-Klaucke W]]
-	[[Category: Rmer, P]]	+	[[Category: Rmer P]]
-	[[Category: Svetlitchnyi, V]]	+	[[Category: Svetlitchnyi V]]
-	[[Category: Thiele, B]]	+	[[Category: Thiele B]]
-	~~[[Category: Cluster some]]~~	+
-	~~[[Category: Nickel]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:21, 22 September 2021

2021-09-22T07:21:44Z

←Older revision		Revision as of 07:21, 22 September 2021
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	<StructureSection load='1ru3' size='340' side='right'caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ru3' size='340' side='right'caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [~~http~~://pdbe.org/1ru3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [https://pdbe.org/1ru3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:47, 20 November 2019

2019-11-20T15:47:43Z

←Older revision		Revision as of 15:47, 20 November 2019
Line 1:		Line 1:

	==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==		==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==
-	<StructureSection load='1ru3' size='340' side='right' caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1ru3' size='340' side='right'caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]		*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]
		+	*[[Acetyl-CoA synthase 3D structures\|Acetyl-CoA synthase 3D structures]]
		+	*[[IronÃ¢ÂÂsulfur proteins\|IronÃ¢ÂÂsulfur proteins]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Carboxydothermus hydrogenoformans]]		[[Category: Carboxydothermus hydrogenoformans]]
		+	[[Category: Large Structures]]
	[[Category: Dobbek, H]]		[[Category: Dobbek, H]]
	[[Category: Huber, R]]		[[Category: Huber, R]]

OCA at 07:08, 8 March 2018

2018-03-08T07:08:52Z

←Older revision		Revision as of 07:08, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==		==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==
	<StructureSection load='1ru3' size='340' side='right' caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ru3' size='340' side='right' caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://pdbe.org/1ru3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://pdbe.org/1ru3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ru3 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ru/1ru3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ru/1ru3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	==See Also==		==See Also==
	*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]		*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]
-	*[[Reverse transcriptase\|Reverse transcriptase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:53, 9 February 2016

2016-02-09T13:53:04Z

←Older revision		Revision as of 13:53, 9 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ru3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:52, 10 September 2015

2015-09-10T22:52:14Z

←Older revision		Revision as of 22:52, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://pdbe.org/1ru3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ru3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:23, 6 January 2015

2015-01-06T07:23:18Z

←Older revision		Revision as of 07:23, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Carboxydothermus hydrogenoformans]]		[[Category: Carboxydothermus hydrogenoformans]]
-	[[Category: Dobbek, H.]]	+	[[Category: Dobbek, H]]
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Meins, T.]]	+	[[Category: Meins, T]]
-	[[Category: Meyer, O.]]	+	[[Category: Meyer, O]]
-	[[Category: Meyer-Klaucke, W.]]	+	[[Category: Meyer-Klaucke, W]]
-	[[Category: Rmer, P.]]	+	[[Category: Rmer, P]]
-	[[Category: Svetlitchnyi, V.]]	+	[[Category: Svetlitchnyi, V]]
-	[[Category: Thiele, B.]]	+	[[Category: Thiele, B]]
	[[Category: Cluster some]]		[[Category: Cluster some]]
	[[Category: Nickel]]		[[Category: Nickel]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 14:13, 29 September 2014

2014-09-29T14:13:51Z

←Older revision		Revision as of 14:13, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1ru3\| PDB=1ru3 \| SCENE= }}~~	+	==Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans==
-	===Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans===	+	<StructureSection load='1ru3' size='340' side='right' caption='[[1ru3]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_14699043}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ru3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RU3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ru3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ru3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ru3 RCSB], [http://www.ebi.ac.uk/pdbsum/1ru3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ru/1ru3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In anaerobic microorganisms employing the acetyl-CoA pathway, acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH) form a complex (ACS/CODH) that catalyzes the synthesis of acetyl-CoA from CO, a methyl group, and CoA. Previously, a [4Fe-4S] cubane bridged to a copper-nickel binuclear site (active site cluster A of the ACS component) was identified in the ACS(Mt)/CODH(Mt) from Moorella thermoacetica whereas another study revealed a nickel-nickel site in the open form of ACS(Mt), and a zink-nickel site in the closed form. The ACS(Ch) of the hydrogenogenic bacterium Carboxydothermus hydrogenoformans was found to exist as an 82.2-kDa monomer as well as in a 1:1 molar complex with the 73.3-kDa CODHIII(Ch). Homogeneous ACS(Ch) and ACS(Ch)/CODHIII(Ch) catalyzed the exchange between [1-(14)C]acetyl-CoA and (12)CO with specific activities of 2.4 or 5.9 micromol of CO per min per mg, respectively, at 70 degrees C and pH 6.0. They also catalyzed the synthesis of acetyl-CoA from CO, methylcobalamin, corrinoid iron-sulfur protein, and CoA with specific activities of 0.14 or 0.91 micromol of acetyl-CoA formed per min per mg, respectively, at 70 degrees C and pH 7.3. The functional cluster A of ACS(Ch) contains a Ni-Ni-[4Fe-4S] site, in which the positions proximal and distal to the cubane are occupied by Ni ions. This result is apparent from a positive correlation of the Ni contents and negative correlations of the Cu or Zn contents with the acetyl-CoA/CO exchange activities of different preparations of monomeric ACS(Ch), a 2.2-A crystal structure of the dithionite-reduced monomer in an open conformation, and x-ray absorption spectroscopy.

-	~~==About this Structure==~~	+	A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans.,Svetlitchnyi V, Dobbek H, Meyer-Klaucke W, Meins T, Thiele B, Romer P, Huber R, Meyer O Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):446-51. Epub 2003 Dec 29. PMID:14699043<ref>PMID:14699043</ref>
-	[~~[1ru3~~]~~] is a 1 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans~~ Carboxydothermus hydrogenoformans]. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1RU3 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]		*[[Acetyl-CoA synthase\|Acetyl-CoA synthase]]
-		+	*[[Reverse transcriptase\|Reverse transcriptase]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:014699043<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Carboxydothermus hydrogenoformans]]		[[Category: Carboxydothermus hydrogenoformans]]
	[[Category: Dobbek, H.]]		[[Category: Dobbek, H.]]

OCA at 09:30, 11 March 2013

2013-03-11T09:30:25Z

←Older revision		Revision as of 09:30, 11 March 2013
Line 1:		Line 1:
-	[[Image:1ru3.png\|left\|200px]]
-
	{{STRUCTURE_1ru3\| PDB=1ru3 \| SCENE= }}		{{STRUCTURE_1ru3\| PDB=1ru3 \| SCENE= }}
-
	===Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans===		===Crystal Structure of the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans===
-
	{{ABSTRACT_PUBMED_14699043}}		{{ABSTRACT_PUBMED_14699043}}

OCA: Protected "1ru3" [edit=sysop:move=sysop]

2013-01-04T10:43:11Z

Protected "1ru3" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:43, 4 January 2013