1rz9 - Revision history

OCA at 06:10, 23 August 2023

2023-08-23T06:10:11Z

←Older revision		Revision as of 06:10, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1rz9' size='340' side='right'caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1rz9' size='340' side='right'caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Adeno-~~associated_virus_-_5~~ Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_5 Adeno-associated virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1m55\|1m55]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">rep ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=82300 Adeno-associated virus - 5])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [https://pdbe.org/1rz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [~~http~~://pdbe.org/1rz9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz9 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9YJC1_9VIRU Q9YJC1_9VIRU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Adeno-associated virus - 5]]	+	[[Category: Adeno-associated virus 5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dyda, F]]	+	[[Category: Dyda F]]
-	[[Category: Hickman~~, A B~~]]	+	[[Category: Hickman AB]]
-	[[Category: Kotin~~, R M~~]]	+	[[Category: Kotin RM]]
-	[[Category: Perez~~, Z N~~]]	+	[[Category: Perez ZN]]
-	[[Category: Ronning~~, D R]]~~	+	[[Category: Ronning DR]]
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Viral protein-dna complex~~]]	+

OCA at 13:36, 16 December 2020

2020-12-16T13:36:45Z

←Older revision		Revision as of 13:36, 16 December 2020
Line 1:		Line 1:

	==Crystal Structure of AAV Rep complexed with the Rep-binding sequence==		==Crystal Structure of AAV Rep complexed with the Rep-binding sequence==
-	<StructureSection load='1rz9' size='340' side='right' caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='1rz9' size='340' side='right'caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1m55\|1m55]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://pdbe.org/1rz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://pdbe.org/1rz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Adeno-associated virus - 5]]		[[Category: Adeno-associated virus - 5]]
		+	[[Category: Large Structures]]
	[[Category: Dyda, F]]		[[Category: Dyda, F]]
	[[Category: Hickman, A B]]		[[Category: Hickman, A B]]

OCA at 07:02, 8 March 2018

2018-03-08T07:02:07Z

←Older revision		Revision as of 07:02, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of AAV Rep complexed with the Rep-binding sequence==		==Crystal Structure of AAV Rep complexed with the Rep-binding sequence==
	<StructureSection load='1rz9' size='340' side='right' caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='1rz9' size='340' side='right' caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://pdbe.org/1rz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://pdbe.org/1rz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz9 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:55, 11 September 2015

2015-09-11T13:55:01Z

←Older revision		Revision as of 13:55, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://pdbe.org/1rz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1rz9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:14, 6 January 2015

2015-01-06T07:14:08Z

←Older revision		Revision as of 07:14, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Adeno-associated virus - 5]]		[[Category: Adeno-associated virus - 5]]
-	[[Category: Dyda, F.]]	+	[[Category: Dyda, F]]
-	[[Category: Hickman, A B.]]	+	[[Category: Hickman, A B]]
-	[[Category: Kotin, R M.]]	+	[[Category: Kotin, R M]]
-	[[Category: Perez, Z N.]]	+	[[Category: Perez, Z N]]
-	[[Category: Ronning, D R.]]	+	[[Category: Ronning, D R]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Viral protein-dna complex]]		[[Category: Viral protein-dna complex]]

OCA at 16:05, 29 September 2014

2014-09-29T16:05:49Z

←Older revision		Revision as of 16:05, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1rz9.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of AAV Rep complexed with the Rep-binding sequence==
		+	<StructureSection load='1rz9' size='340' side='right' caption='[[1rz9]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1m55\|1m55]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rep ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=82300 Adeno-associated virus - 5])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz9 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms. At the Rep binding site, five Rep monomers bind five tetranucleotide direct repeats; each repeat is recognized by two Rep monomers from opposing faces of the DNA. Stem-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.

-	~~{{STRUCTURE_1rz9\| PDB=1rz9 \| SCENE= }}~~	+	The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces.,Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F Mol Cell. 2004 Feb 13;13(3):403-14. PMID:14967147<ref>PMID:14967147</ref>

-	~~===Crystal Structure~~ of ~~AAV Rep complexed with~~ the ~~Rep-binding sequence===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14967147}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1rz9]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014967147<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Adeno-associated virus - 5]]		[[Category: Adeno-associated virus - 5]]
	[[Category: Dyda, F.]]		[[Category: Dyda, F.]]

OCA: Protected "1rz9" [edit=sysop:move=sysop]

2013-01-04T10:33:54Z

Protected "1rz9" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:33, 4 January 2013

OCA at 10:33, 4 January 2013

2013-01-04T10:33:51Z

←Older revision		Revision as of 10:33, 4 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1rz9.png\|left\|200px]]		[[Image:1rz9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1rz9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1rz9\| PDB=1rz9 \| SCENE= }}		{{STRUCTURE_1rz9\| PDB=1rz9 \| SCENE= }}

	===Crystal Structure of AAV Rep complexed with the Rep-binding sequence===		===Crystal Structure of AAV Rep complexed with the Rep-binding sequence===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14967147}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14967147 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14967147}}		{{ABSTRACT_PUBMED_14967147}}

	==About this Structure==		==About this Structure==
-	~~1RZ9~~ is a 7 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].	+	[[1rz9]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14967147~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014967147</ref><references group="xtra"/>
	[[Category: Adeno-associated virus - 5]]		[[Category: Adeno-associated virus - 5]]
	[[Category: Dyda, F.]]		[[Category: Dyda, F.]]
Line 31:		Line 19:
	[[Category: Ronning, D R.]]		[[Category: Ronning, D R.]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
-		+	[[Category: Viral protein-dna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:04:00 2009''~~	+

OCA at 22:04, 17 February 2009

2009-02-17T22:04:01Z

←Older revision		Revision as of 22:04, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1RZ9 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].	+	1RZ9 is a 7 chains structure of sequences from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ9 OCA].

	==Reference==		==Reference==
-	The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14967147 14967147]~~	+	<ref group="xtra">PMID:14967147</ref><references group="xtra"/>
	[[Category: Adeno-associated virus - 5]]		[[Category: Adeno-associated virus - 5]]
-	[[Category: Single protein]]
	[[Category: Dyda, F.]]		[[Category: Dyda, F.]]
	[[Category: Hickman, A B.]]		[[Category: Hickman, A B.]]
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	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 07~~:22:~~24 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:04:00 2009''

OCA at 04:22, 29 July 2008

2008-07-29T04:22:27Z

←Older revision		Revision as of 04:22, 29 July 2008
Line 1:		Line 1:
-	[[Image:1rz9.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1rz9.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1rz9\| PDB=1rz9 \| SCENE= }}		{{STRUCTURE_1rz9\| PDB=1rz9 \| SCENE= }}

-	~~'''~~Crystal Structure of AAV Rep complexed with the Rep-binding sequence~~'''~~	+	===Crystal Structure of AAV Rep complexed with the Rep-binding sequence===


-	~~==Overview==~~	+	<!--
-	Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, ~~we have determined~~ the ~~structures of~~ the ~~Rep endonuclease domain separately complexed with two DNA substrates~~: ~~the Rep binding site within the viral inverted terminal repeat and one of the terminal hairpin arms~~. ~~At the Rep binding site~~, ~~five Rep monomers bind five tetranucleotide direct repeats; each repeat~~ is ~~recognized by two Rep monomers from opposing faces of~~ the ~~DNA~~. ~~Stem~~-loop binding involves a protein interface on the opposite side of the molecule from the active site where ssDNA is cleaved. Rep therefore has three distinct binding sites within its endonuclease domain for its different DNA substrates. Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration.	+	The line below this paragraph, {{ABSTRACT_PUBMED_14967147}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14967147 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14967147}}

	==About this Structure==		==About this Structure==
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	[[Category: Ronning, D R.]]		[[Category: Ronning, D R.]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:05:45 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:22:24 2008''