1s5b - Revision history

OCA at 06:12, 23 August 2023

2023-08-23T06:12:57Z

←Older revision		Revision as of 06:12, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1s5b' size='340' side='right'caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>		<StructureSection load='1s5b' size='340' side='right'caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S5B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.13Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1xtc\|1xtc]]~~, [[~~1lts~~\|~~1lts]], [[1ltg\|1ltg]], [[1lta\|1lta~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CTXA, TOXA, VC1457 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~666~~ "~~Bacillo virgola del Koch~~" ~~Trevisan 1884]), CTXB, TOXB, VC1456 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [https://pdbe.org/1s5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [https://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [https://pdbe.org/1s5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [https://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CHTA_VIBCH CHTA_VIBCH]] The A1 chain catalyzes the ADP-ribosylation of Gs alpha, a GTP-binding regulatory protein, to activate the adenylate cyclase. This leads to an overproduction of cAMP and eventually to a hypersecretion of chloride and bicarbonate followed by water, resulting in the characteristic cholera stool. The A2 chain tethers A1 to the pentameric ring. [[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.	+	[https://www.uniprot.org/uniprot/CHTA_VIBCH CHTA_VIBCH] The A1 chain catalyzes the ADP-ribosylation of Gs alpha, a GTP-binding regulatory protein, to activate the adenylate cyclase. This leads to an overproduction of cAMP and eventually to a hypersecretion of chloride and bicarbonate followed by water, resulting in the characteristic cholera stool. The A2 chain tethers A1 to the pentameric ring.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Amaya, E I~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: ~~Hol, W G~~]]	+	[[Category: Amaya EI]]
-	[[Category: ~~Holmes, R K~~]]	+	[[Category: Hol WG]]
-	[[Category: ~~Jobling, M G~~]]	+	[[Category: Holmes RK]]
-	[[Category: ~~Neal, C J.O~~]]	+	[[Category: Jobling MG]]
-	[[Category: ~~Ab5 toxin]]~~	+	[[Category: O'Neal CJ]]
-	~~[[Category: Adp ribose transferase]]~~	+
-	~~[[Category: Cholera toxin]]~~	+
-	~~[[Category: Heat-labile enterotoxin]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:30, 27 October 2021

2021-10-27T15:30:39Z

OCA at 13:40, 16 December 2020

2020-12-16T13:40:47Z

←Older revision		Revision as of 13:40, 16 December 2020
Line 1:		Line 1:

	==Cholera holotoxin with an A-subunit Y30S mutation Form 3==		==Cholera holotoxin with an A-subunit Y30S mutation Form 3==
-	<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>	+	<StructureSection load='1s5b' size='340' side='right'caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1S5B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://pdbe.org/1s5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://pdbe.org/1s5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Cholera toxin\|Cholera toxin]]	+	*[[Cholera toxin 3D structures\|Cholera toxin 3D structures]]
	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]		*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]
	== References ==		== References ==
Line 40:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Amaya, E I]]		[[Category: Amaya, E I]]
	[[Category: Hol, W G]]		[[Category: Hol, W G]]

OCA at 07:14, 8 March 2018

2018-03-08T07:14:51Z

←Older revision		Revision as of 07:14, 8 March 2018
Line 1:		Line 1:
		+
	==Cholera holotoxin with an A-subunit Y30S mutation Form 3==		==Cholera holotoxin with an A-subunit Y30S mutation Form 3==
	<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>		<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://pdbe.org/1s5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://pdbe.org/1s5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s5/1s5b_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s5/1s5b_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s5b ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:10, 11 September 2015

2015-09-11T17:10:41Z

←Older revision		Revision as of 17:10, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>		<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae Vibrio cholerae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S5B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 ~~Vibrio cholerae~~]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 ~~Vibrio cholerae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://pdbe.org/1s5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1s5b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae~~]]	+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Amaya, E I]]		[[Category: Amaya, E I]]
	[[Category: Hol, W G]]		[[Category: Hol, W G]]

OCA at 19:25, 25 December 2014

2014-12-25T19:25:42Z

←Older revision		Revision as of 19:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S5B FirstGlance]. <br>		<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHTA_VIBCH CHTA_VIBCH]] The A1 chain catalyzes the ADP-ribosylation of Gs alpha, a GTP-binding regulatory protein, to activate the adenylate cyclase. This leads to an overproduction of cAMP and eventually to a hypersecretion of chloride and bicarbonate followed by water, resulting in the characteristic cholera stool. The A2 chain tethers A1 to the pentameric ring. [[http://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
-	[[Category: Amaya, E I.]]	+	[[Category: Amaya, E I]]
-	[[Category: Hol, W G.]]	+	[[Category: Hol, W G]]
-	[[Category: Holmes, R K.]]	+	[[Category: Holmes, R K]]
-	[[Category: Jobling, M G.]]	+	[[Category: Jobling, M G]]
-	[[Category: Neal, C J.O.]]	+	[[Category: Neal, C J.O]]
	[[Category: Ab5 toxin]]		[[Category: Ab5 toxin]]
	[[Category: Adp ribose transferase]]		[[Category: Adp ribose transferase]]

OCA at 19:56, 28 September 2014

2014-09-28T19:56:20Z

←Older revision		Revision as of 19:56, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1s5b.~~png~~\|~~left~~\|200px]]	+	==Cholera holotoxin with an A-subunit Y30S mutation Form 3==
		+	<StructureSection load='1s5b' size='340' side='right' caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae]), CTXB, TOXB, VC1456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [http://www.ebi.ac.uk/pdbsum/1s5b PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s5/1s5b_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cholera toxin (CT) is a heterohexameric bacterial protein toxin belonging to a larger family of A/B ADP-ribosylating toxins. Each of these toxins undergoes limited proteolysis and/or disulfide bond reduction to form the enzymatically active toxic fragment. Nicking and reduction render both CT and the closely related heat-labile enterotoxin from Escherichia coli (LT) unstable in solution, thus far preventing a full structural understanding of the conformational changes resulting from toxin activation. We present the first structural glimpse of an active CT in structures from three crystal forms of a single-site A-subunit CT variant, Y30S, which requires no activational modifications for full activity. We also redetermined the structure of the wild-type, proenzyme CT from two crystal forms, both of which exhibit (i) better geometry and (ii) a different A2 "tail" conformation than the previously determined structure [Zhang et al. (1995) J. Mol. Biol. 251, 563-573]. Differences between wild-type CT and active CTY30S are observed in A-subunit loop regions that had been previously implicated in activation by analysis of the structure of an LT A-subunit R7K variant [van den Akker et al. (1995) Biochemistry 34, 10996-11004]. The 25-36 activation loop is disordered in CTY30S, while the 47-56 active site loop displays varying degrees of order in the three CTY30S structures, suggesting that disorder in the activation loop predisposes the active site loop to a greater degree of flexibility than that found in unactivated wild-type CT. On the basis of these six new views of the CT holotoxin, we propose a model for how the activational modifications experienced by wild-type CT are communicated to the active site.

-	~~{{STRUCTURE_1s5b\| PDB=1s5b \| SCENE= }}~~	+	Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism.,O'Neal CJ, Amaya EI, Jobling MG, Holmes RK, Hol WG Biochemistry. 2004 Apr 6;43(13):3772-82. PMID:15049684<ref>PMID:15049684</ref>

-	~~===Cholera holotoxin with an A-subunit Y30S mutation Form 3===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15049684}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1s5b]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vibrio_cholerae Vibrio cholerae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA].~~	+

	==See Also==		==See Also==
	*[[Cholera toxin\|Cholera toxin]]		*[[Cholera toxin\|Cholera toxin]]
	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]		*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015049684<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Amaya, E I.]]		[[Category: Amaya, E I.]]

OCA at 20:31, 20 October 2012

2012-10-20T20:31:34Z

←Older revision		Revision as of 20:31, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1s5b]] is a 6 chain structure ~~of [[Cholera toxin]] and [[User:David Solfiell/sandbox 1]]~~ with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA].	+	[[1s5b]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA].

	==See Also==		==See Also==

OCA at 10:46, 27 July 2012

2012-07-27T10:46:13Z

←Older revision		Revision as of 10:46, 27 July 2012
Line 1:		Line 1:
	[[Image:1s5b.png\|left\|200px]]		[[Image:1s5b.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1s5b", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1s5b\| PDB=1s5b \| SCENE= }}		{{STRUCTURE_1s5b\| PDB=1s5b \| SCENE= }}

	===Cholera holotoxin with an A-subunit Y30S mutation Form 3===		===Cholera holotoxin with an A-subunit Y30S mutation Form 3===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15049684}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15049684 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15049684}}		{{ABSTRACT_PUBMED_15049684}}

	==About this Structure==		==About this Structure==
-	[[1s5b]] is a 6 chain structure of [[Cholera toxin]] with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA].	+	[[1s5b]] is a 6 chain structure of [[Cholera toxin]] and [[User:David Solfiell/sandbox 1]] with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA].

	==See Also==		==See Also==
-	*[[Cholera toxin]]	+	*[[Cholera toxin\|Cholera toxin]]
		+	*[[User:David Solfiell/sandbox 1\|User:David Solfiell/sandbox 1]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15049684~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015049684</ref><references group="xtra"/>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Amaya, E I.]]		[[Category: Amaya, E I.]]
Line 37:		Line 27:
	[[Category: Heat-labile enterotoxin]]		[[Category: Heat-labile enterotoxin]]
	[[Category: Toxin]]		[[Category: Toxin]]
-	[[Category: ~~Transerase~~]]	+	[[Category: Transferase]]

OCA: Protected "1s5b" [edit=sysop:move=sysop]

2010-12-26T09:26:37Z

Protected "1s5b" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:26, 26 December 2010

←Older revision		Revision as of 15:30, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1s5b' size='340' side='right'caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>		<StructureSection load='1s5b' size='340' side='right'caption='[[1s5b]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1S5B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s5b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S5B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xtc\|1xtc]], [[1lts\|1lts]], [[1ltg\|1ltg]], [[1lta\|1lta]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTXA, TOXA, VC1457 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884]), CTXB, TOXB, VC1456 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [~~http~~://pdbe.org/1s5b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5b OCA], [https://pdbe.org/1s5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s5b RCSB], [https://www.ebi.ac.uk/pdbsum/1s5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHTA_VIBCH CHTA_VIBCH]] The A1 chain catalyzes the ADP-ribosylation of Gs alpha, a GTP-binding regulatory protein, to activate the adenylate cyclase. This leads to an overproduction of cAMP and eventually to a hypersecretion of chloride and bicarbonate followed by water, resulting in the characteristic cholera stool. The A2 chain tethers A1 to the pentameric ring. [[~~http~~://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.	+	[[https://www.uniprot.org/uniprot/CHTA_VIBCH CHTA_VIBCH]] The A1 chain catalyzes the ADP-ribosylation of Gs alpha, a GTP-binding regulatory protein, to activate the adenylate cyclase. This leads to an overproduction of cAMP and eventually to a hypersecretion of chloride and bicarbonate followed by water, resulting in the characteristic cholera stool. The A2 chain tethers A1 to the pentameric ring. [[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]