1se0 - Revision history

OCA at 08:30, 14 February 2024

2024-02-14T08:30:06Z

←Older revision		Revision as of 08:30, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1se0' size='340' side='right'caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1se0' size='340' side='right'caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1sdz\|1sdz]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [https://pdbe.org/1se0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [https://www.ebi.ac.uk/pdbsum/1se0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1se0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [~~http~~://pdbe.org/1se0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1se0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1se0 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~IAP1_DROME IAP1_DROME]~~] Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Nc, and effector caspases ~~ICE~~ and ~~DCP~~-1. Acts as a ~~NEDD8~~-E3 ubiquitin-protein ligase for ~~ICE~~. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and W-dependent cell death in the eye. Interaction of th with Nc is required to suppress Nc-mediated cell death~~; th~~-mediated ubiquitination of Nc. Acts as a positive regulator of Wnt signaling.<ref>PMID:~~8548811~~</ref> <ref>PMID:17397804</ref> <ref>PMID:18259196</ref> <ref>PMID:21145488</ref> <ref>PMID:22304967</ref> <ref>PMID:~~14517550~~</ref>	+	[https://www.uniprot.org/uniprot/DIAP1_DROME DIAP1_DROME] Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling.<ref>PMID:14517550</ref> <ref>PMID:17397804</ref> <ref>PMID:18259196</ref> <ref>PMID:21145488</ref> <ref>PMID:22304967</ref> <ref>PMID:8548811</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1se0 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1se0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Drosophila melanogaster inhibitor of apoptosis protein DIAP1 suppresses apoptosis in part through inhibition of the effector caspase DrICE. The pro-death proteins Reaper, Hid and Grim (RHG) induce apoptosis by antagonizing DIAP1 function. However, the underlying molecular mechanisms remain unknown. Here we demonstrate that DIAP1 directly inhibits the catalytic activity of DrICE through its BIR1 domain and this inhibition is countered effectively by the RHG proteins. Inhibition of DrICE by DIAP1 occurs only after the cleavage of its N-terminal 20 amino acids and involves a conserved surface groove on BIR1. Crystal structures of BIR1 bound to the RHG peptides show that the RHG proteins use their N-terminal IAP-binding motifs to bind to the same surface groove, hence relieving DIAP1-mediated inhibition of DrICE. These studies define novel molecular mechanisms for the inhibition and activation of a representative D. melanogaster effector caspase.
-
-	Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim.,Yan N, Wu JW, Chai J, Li W, Shi Y Nat Struct Mol Biol. 2004 May;11(5):420-8. Epub 2004 Apr 25. PMID:15107838<ref>PMID:15107838</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1se0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Shi, Y]]	+	[[Category: Shi Y]]
-	[[Category: Wu~~, J W~~]]	+	[[Category: Wu JW]]
-	[[Category: Yan, N]]	+	[[Category: Yan N]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Bir]]~~	+
-	~~[[Category: Caspase]]~~	+
-	~~[[Category: Grim]]~~	+
-	~~[[Category: Iap~~]]	+

OCA at 11:21, 24 December 2020

2020-12-24T11:21:38Z

←Older revision		Revision as of 11:21, 24 December 2020
Line 1:		Line 1:

	==Crystal structure of DIAP1 BIR1 bound to a Grim peptide==		==Crystal structure of DIAP1 BIR1 bound to a Grim peptide==
-	<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='1se0' size='340' side='right'caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1sdz\|1sdz]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://pdbe.org/1se0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://pdbe.org/1se0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Shi, Y]]		[[Category: Shi, Y]]
	[[Category: Wu, J W]]		[[Category: Wu, J W]]

OCA at 06:55, 8 March 2018

2018-03-08T06:55:17Z

←Older revision		Revision as of 06:55, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of DIAP1 BIR1 bound to a Grim peptide==		==Crystal structure of DIAP1 BIR1 bound to a Grim peptide==
	<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://pdbe.org/1se0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://pdbe.org/1se0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/se/1se0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/se/1se0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1se0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:46, 11 September 2015

2015-09-11T07:46:34Z

←Older revision		Revision as of 07:46, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://pdbe.org/1se0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1se0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Shi, Y]]		[[Category: Shi, Y]]
	[[Category: Wu, J W]]		[[Category: Wu, J W]]

OCA at 13:25, 25 December 2014

2014-12-25T13:25:49Z

←Older revision		Revision as of 13:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IAP1_DROME IAP1_DROME]] Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Nc, and effector caspases ICE and DCP-1. Acts as a NEDD8-E3 ubiquitin-protein ligase for ICE. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and W-dependent cell death in the eye. Interaction of th with Nc is required to suppress Nc-mediated cell death; th-mediated ubiquitination of Nc. Acts as a positive regulator of Wnt signaling.<ref>PMID:8548811</ref> <ref>PMID:17397804</ref> <ref>PMID:18259196</ref> <ref>PMID:21145488</ref> <ref>PMID:22304967</ref> <ref>PMID:14517550</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Shi, Y.]]	+	[[Category: Shi, Y]]
-	[[Category: Wu, J W.]]	+	[[Category: Wu, J W]]
-	[[Category: Yan, N.]]	+	[[Category: Yan, N]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Bir]]		[[Category: Bir]]

OCA at 16:01, 29 September 2014

2014-09-29T16:01:35Z

←Older revision		Revision as of 16:01, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1se0.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of DIAP1 BIR1 bound to a Grim peptide==
		+	<StructureSection load='1se0' size='340' side='right' caption='[[1se0]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SE0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sdz\|1sdz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IAP1, TH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1se0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1se0 RCSB], [http://www.ebi.ac.uk/pdbsum/1se0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/se/1se0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Drosophila melanogaster inhibitor of apoptosis protein DIAP1 suppresses apoptosis in part through inhibition of the effector caspase DrICE. The pro-death proteins Reaper, Hid and Grim (RHG) induce apoptosis by antagonizing DIAP1 function. However, the underlying molecular mechanisms remain unknown. Here we demonstrate that DIAP1 directly inhibits the catalytic activity of DrICE through its BIR1 domain and this inhibition is countered effectively by the RHG proteins. Inhibition of DrICE by DIAP1 occurs only after the cleavage of its N-terminal 20 amino acids and involves a conserved surface groove on BIR1. Crystal structures of BIR1 bound to the RHG peptides show that the RHG proteins use their N-terminal IAP-binding motifs to bind to the same surface groove, hence relieving DIAP1-mediated inhibition of DrICE. These studies define novel molecular mechanisms for the inhibition and activation of a representative D. melanogaster effector caspase.

-	~~{{STRUCTURE_1se0\| PDB=1se0 \| SCENE= }}~~	+	Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim.,Yan N, Wu JW, Chai J, Li W, Shi Y Nat Struct Mol Biol. 2004 May;11(5):420-8. Epub 2004 Apr 25. PMID:15107838<ref>PMID:15107838</ref>

-	~~===Crystal structure~~ of ~~DIAP1 BIR1 bound to a Grim peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15107838}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1se0]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015107838<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Shi, Y.]]		[[Category: Shi, Y.]]

OCA: Protected "1se0" [edit=sysop:move=sysop]

2013-01-04T10:24:41Z

Protected "1se0" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:24, 4 January 2013

OCA at 10:24, 4 January 2013

2013-01-04T10:24:38Z

←Older revision		Revision as of 10:24, 4 January 2013
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-	{{Seed}}
	[[Image:1se0.png\|left\|200px]]		[[Image:1se0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1se0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1se0\| PDB=1se0 \| SCENE= }}		{{STRUCTURE_1se0\| PDB=1se0 \| SCENE= }}

	===Crystal structure of DIAP1 BIR1 bound to a Grim peptide===		===Crystal structure of DIAP1 BIR1 bound to a Grim peptide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15107838}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15107838 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15107838}}		{{ABSTRACT_PUBMED_15107838}}

	==About this Structure==		==About this Structure==
-	~~1SE0~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].	+	[[1se0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15107838~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015107838</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Shi, Y.]]		[[Category: Shi, Y.]]
Line 33:		Line 21:
	[[Category: Grim]]		[[Category: Grim]]
	[[Category: Iap]]		[[Category: Iap]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:22:03 2009''

OCA at 15:22, 17 February 2009

2009-02-17T15:22:05Z

←Older revision		Revision as of 15:22, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1SE0 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].	+	1SE0 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].

	==Reference==		==Reference==
-	~~Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim., Yan N, Wu JW, Chai J, Li W, Shi Y, Nat Struct Mol Biol. 2004 May;11(5):420-8. Epub 2004 Apr 25.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15107838 15107838]~~	+	<ref group="xtra">PMID:15107838</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Single protein]]
	[[Category: Shi, Y.]]		[[Category: Shi, Y.]]
	[[Category: Wu, J W.]]		[[Category: Wu, J W.]]
Line 35:		Line 34:
	[[Category: Iap]]		[[Category: Iap]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 00~~:19:~~11 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:22:03 2009''

OCA at 21:19, 28 July 2008

2008-07-28T21:19:13Z

←Older revision		Revision as of 21:19, 28 July 2008
Line 1:		Line 1:
-	[[Image:1se0.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1se0.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1se0\| PDB=1se0 \| SCENE= }}		{{STRUCTURE_1se0\| PDB=1se0 \| SCENE= }}

-	~~'''~~Crystal structure of DIAP1 BIR1 bound to a Grim peptide~~'''~~	+	===Crystal structure of DIAP1 BIR1 bound to a Grim peptide===


-	~~==Overview==~~	+	<!--
-	The ~~Drosophila melanogaster inhibitor of apoptosis protein DIAP1 suppresses apoptosis in part through inhibition of~~ the ~~effector caspase DrICE~~. ~~The pro-death proteins Reaper, Hid and Grim (RHG~~) ~~induce apoptosis by antagonizing DIAP1 function. However~~, ~~the underlying molecular mechanisms remain unknown. Here we demonstrate that DIAP1 directly inhibits the catalytic activity of DrICE through its BIR1 domain and this inhibition~~ is ~~countered effectively by~~ the ~~RHG proteins~~. ~~Inhibition of DrICE by DIAP1 occurs only after the cleavage of its N~~-~~terminal 20 amino acids and involves a conserved surface groove on BIR1. Crystal structures of BIR1 bound to the RHG peptides show that the RHG proteins use their N~~-terminal IAP-binding motifs to bind to the same surface groove, hence relieving DIAP1-mediated inhibition of DrICE. These studies define novel molecular mechanisms for the inhibition and activation of a representative D. melanogaster effector caspase.	+	The line below this paragraph, {{ABSTRACT_PUBMED_15107838}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15107838 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15107838}}

	==About this Structure==		==About this Structure==
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	[[Category: Grim]]		[[Category: Grim]]
	[[Category: Iap]]		[[Category: Iap]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:35:38 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:19:11 2008''