1sjw - Revision history

OCA at 08:31, 14 February 2024

2024-02-14T08:31:26Z

←Older revision		Revision as of 08:31, 14 February 2024
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	<StructureSection load='1sjw' size='340' side='right'caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='1sjw' size='340' side='right'caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1442 As 4.1442~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1SJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NGV:METHYL+5,7~~-~~DIHYDROXY-2-METHYL-4~~,~~6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-~~1~~-CARBOXYLATE'>NGV</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=38314 AS~~ 4~~.1442])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [~~http~~://pdbe.org/1sjw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [https://pdbe.org/1sjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [https://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SNOAL_STRNO SNOAL_STRNO] Involved in the biosynthesis of the aromatic polyketide antibiotic nogalamycin. Catalyzes the formation of nogalaviketone from nogalonic acid methyl ester (NAME), the last ring-closure step in the biosynthesis of nogalamycin.<ref>PMID:10639368</ref> <ref>PMID:15071504</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjw ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The crystal structure of SnoaL, involved in nogalamycin biosynthesis, with a bound product, has been determined to 1.35 A resolution. The fold of the subunit can be described as a distorted alpha+beta barrel, and the ligand is bound in the hydrophobic interior of the barrel. The 3D structure and site-directed mutagenesis experiments reveal that the mechanism of the intramolecular aldol condensation catalysed by SnoaL is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended pi system of the substrate. These polyketide cyclases thus form of family of enzymes with a unique catalytic strategy for aldol condensation.
-
-	Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.,Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G EMBO J. 2004 May 5;23(9):1911-21. Epub 2004 Apr 8. PMID:15071504<ref>PMID:15071504</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1sjw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1442]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jansson, A]]	+	[[Category: Streptomyces nogalater]]
-	[[Category: Kallio, P]]	+	[[Category: Jansson A]]
-	[[Category: Mantsala, P]]	+	[[Category: Kallio P]]
-	[[Category: Neimi, J]]	+	[[Category: Mantsala P]]
-	~~[[Category: SPINE, Structural Proteomics in Europe~~]]	+	[[Category: Neimi J]]
-	[[Category: Schneider, G]]	+	[[Category: Schneider G]]
-	[[Category: Sultana, A]]	+	[[Category: Sultana A]]
-	[[Category: Wang~~, J S]]~~	+	[[Category: Wang JS]]
-	~~[[Category: Aldol condensation]]~~	+
-	~~[[Category: Anthracycline]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Nogalamycin]]~~	+
-	~~[[Category: Snoal]]~~	+
-	~~[[Category: Spine]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Structural proteomics in europe~~]]	+

OCA at 11:28, 24 December 2020

2020-12-24T11:28:20Z

←Older revision		Revision as of 11:28, 24 December 2020
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	==Structure of polyketide cyclase SnoaL==		==Structure of polyketide cyclase SnoaL==
-	<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>	+	<StructureSection load='1sjw' size='340' side='right'caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://pdbe.org/1sjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://pdbe.org/1sjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1442]]		[[Category: As 4 1442]]
		+	[[Category: Large Structures]]
	[[Category: Jansson, A]]		[[Category: Jansson, A]]
	[[Category: Kallio, P]]		[[Category: Kallio, P]]

OCA at 08:49, 12 October 2017

2017-10-12T08:49:35Z

←Older revision		Revision as of 08:49, 12 October 2017
Line 1:		Line 1:
		+
	==Structure of polyketide cyclase SnoaL==		==Structure of polyketide cyclase SnoaL==
	<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://pdbe.org/1sjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://pdbe.org/1sjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:42, 11 September 2015

2015-09-11T10:42:56Z

←Older revision		Revision as of 10:42, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_nogalater Streptomyces nogalater~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 ~~Streptomyces nogalater~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://pdbe.org/1sjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1sjw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces nogalater~~]]	+	[[Category: As 4 1442]]
	[[Category: Jansson, A]]		[[Category: Jansson, A]]
	[[Category: Kallio, P]]		[[Category: Kallio, P]]

OCA at 07:04, 6 January 2015

2015-01-06T07:04:24Z

←Older revision		Revision as of 07:04, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SJW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
-	[[Category: Jansson, A.]]	+	[[Category: Jansson, A]]
-	[[Category: Kallio, P.]]	+	[[Category: Kallio, P]]
-	[[Category: Mantsala, P.]]	+	[[Category: Mantsala, P]]
-	[[Category: Neimi, J.]]	+	[[Category: Neimi, J]]
-	[[Category: SPINE, Structural Proteomics in Europe.]]	+	[[Category: SPINE, Structural Proteomics in Europe]]
-	[[Category: Schneider, G.]]	+	[[Category: Schneider, G]]
-	[[Category: Sultana, A.]]	+	[[Category: Sultana, A]]
-	[[Category: Wang, J S.]]	+	[[Category: Wang, J S]]
	[[Category: Aldol condensation]]		[[Category: Aldol condensation]]
	[[Category: Anthracycline]]		[[Category: Anthracycline]]

OCA at 14:04, 29 September 2014

2014-09-29T14:04:38Z

←Older revision		Revision as of 14:04, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1sjw.~~png~~\|~~left~~\|200px]]	+	==Structure of polyketide cyclase SnoaL==
		+	<StructureSection load='1sjw' size='340' side='right' caption='[[1sjw]], [[Resolution\|resolution]] 1.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The crystal structure of SnoaL, involved in nogalamycin biosynthesis, with a bound product, has been determined to 1.35 A resolution. The fold of the subunit can be described as a distorted alpha+beta barrel, and the ligand is bound in the hydrophobic interior of the barrel. The 3D structure and site-directed mutagenesis experiments reveal that the mechanism of the intramolecular aldol condensation catalysed by SnoaL is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended pi system of the substrate. These polyketide cyclases thus form of family of enzymes with a unique catalytic strategy for aldol condensation.

-	~~{{STRUCTURE_1sjw\| PDB=1sjw \| SCENE= }}~~	+	Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.,Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G EMBO J. 2004 May 5;23(9):1911-21. Epub 2004 Apr 8. PMID:15071504<ref>PMID:15071504</ref>

-	~~===Structure~~ of ~~polyketide cyclase SnoaL===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15071504}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1sjw]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_nogalater Streptomyces nogalater]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015071504<~~/~~ref~~><~~ref group="xtra">PMID:015616961</ref><references group="xtra"~~/>	+
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Jansson, A.]]		[[Category: Jansson, A.]]

OCA: Protected "1sjw" [edit=sysop:move=sysop]

2013-01-04T10:24:02Z

Protected "1sjw" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:24, 4 January 2013

OCA at 10:24, 4 January 2013

2013-01-04T10:24:00Z

←Older revision		Revision as of 10:24, 4 January 2013
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-	{{Seed}}
	[[Image:1sjw.png\|left\|200px]]		[[Image:1sjw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1sjw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1sjw\| PDB=1sjw \| SCENE= }}		{{STRUCTURE_1sjw\| PDB=1sjw \| SCENE= }}

	===Structure of polyketide cyclase SnoaL===		===Structure of polyketide cyclase SnoaL===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15071504}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15071504 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15071504}}		{{ABSTRACT_PUBMED_15071504}}

	==About this Structure==		==About this Structure==
-	~~1SJW~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].	+	[[1sjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15071504~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015071504</ref><ref group="xtra">PMID:015616961</ref><references group="xtra"/>
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Jansson, A.]]		[[Category: Jansson, A.]]
Line 35:		Line 23:
	[[Category: Aldol condensation]]		[[Category: Aldol condensation]]
	[[Category: Anthracycline]]		[[Category: Anthracycline]]
-	[[Category: ~~Crystal structure~~]]	+	[[Category: Lyase]]
	[[Category: Nogalamycin]]		[[Category: Nogalamycin]]
	[[Category: Snoal]]		[[Category: Snoal]]
Line 41:		Line 29:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Structural proteomics in europe]]		[[Category: Structural proteomics in europe]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:11:52 2009''

OCA at 18:11, 17 February 2009

2009-02-17T18:11:53Z

←Older revision		Revision as of 18:11, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1SJW is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].	+	1SJW is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].

	==Reference==		==Reference==
-	Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation., Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G, EMBO J. 2004 May 5;23(9):1911-21. Epub 2004 Apr 8. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15071504 15071504]~~	+	<ref group="xtra">PMID:15071504</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Jansson, A.]]		[[Category: Jansson, A.]]
Line 43:		Line 42:
	[[Category: Structural proteomics in europe]]		[[Category: Structural proteomics in europe]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:03:~~22 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:11:52 2009''

OCA at 01:03, 29 July 2008

2008-07-29T01:03:33Z

←Older revision		Revision as of 01:03, 29 July 2008
Line 1:		Line 1:
-	[[Image:1sjw.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1sjw.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1sjw\| PDB=1sjw \| SCENE= }}		{{STRUCTURE_1sjw\| PDB=1sjw \| SCENE= }}

-	~~'''~~Structure of polyketide cyclase SnoaL~~'''~~	+	===Structure of polyketide cyclase SnoaL===


-	~~==Overview==~~	+	<!--
-	SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The ~~crystal structure of SnoaL~~, ~~involved in nogalamycin biosynthesis~~, ~~with a bound product, has been determined~~ to ~~1.35 A resolution. The fold of~~ the ~~subunit can be described~~ as ~~a distorted alpha+beta barrel, and the ligand is bound in the hydrophobic interior of the barrel~~. The 3D structure and site-directed mutagenesis experiments reveal that the mechanism of the intramolecular aldol condensation catalysed by SnoaL is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. ~~The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate~~) ~~intermediate~~ is ~~mainly achieved by~~ the ~~delocalisation of the electron pair over the extended pi system of the substrate. These polyketide cyclases thus form of family of enzymes with a unique catalytic strategy for aldol condensation~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_15071504}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15071504 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15071504}}

	==About this Structure==		==About this Structure==
Line 38:		Line 42:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Structural proteomics in europe]]		[[Category: Structural proteomics in europe]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Apr 13 08~~:10:59 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:03:22 2008''