1szn - Revision history

OCA at 08:36, 1 May 2024

2024-05-01T08:36:56Z

←Older revision		Revision as of 08:36, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='1szn' size='340' side='right'caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='1szn' size='340' side='right'caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Hypocrea_jecorina Hypocrea jecorina~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.54Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [https://pdbe.org/1szn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [https://www.ebi.ac.uk/pdbsum/1szn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [https://pdbe.org/1szn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [https://www.ebi.ac.uk/pdbsum/1szn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q92456_HYPJE Q92456_HYPJE] Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides.[ARBA:ARBA00003969]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.
-
-	Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.,Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043<ref>PMID:15136043</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Galactosidase 3D structures\|Galactosidase 3D structures]]		*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alpha-galactosidase]]
-	[[Category: Hypocrea jecorina]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Eneyskaya, E V~~]]	+	[[Category: Trichoderma reesei]]
-	[[Category: ~~Golubev, A M~~]]	+	[[Category: Brando Neto JR]]
-	[[Category: ~~Kulminskaya, A A~~]]	+	[[Category: Eneyskaya EV]]
-	[[Category: ~~Nagem, R A.P~~]]	+	[[Category: Golubev AM]]
-	[[Category: ~~Neto, J R.Brando~~]]	+	[[Category: Kulminskaya AA]]
-	[[Category: ~~Neustroev, K N~~]]	+	[[Category: Nagem RAP]]
-	[[Category: ~~Polikarpov, I~~]]	+	[[Category: Neustroev KN]]
-	[[Category: ~~Shabalin, K A~~]]	+	[[Category: Polikarpov I]]
-	[[Category: ~~Ev, A N.~~Savel]]	+	[[Category: Savel'ev AN]]
-	[[Category: ~~Glycoprotein]]~~	+	[[Category: Shabalin KA]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Two domain~~]]	+

OCA at 09:02, 21 April 2021

2021-04-21T09:02:24Z

←Older revision		Revision as of 09:02, 21 April 2021
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	<StructureSection load='1szn' size='340' side='right'caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='1szn' size='340' side='right'caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_13631 Atcc 13631~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [~~http~~://pdbe.org/1szn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1szn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1szn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [https://pdbe.org/1szn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [https://www.ebi.ac.uk/pdbsum/1szn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
-	[[Category: ~~Atcc 13631~~]]	+	[[Category: Hypocrea jecorina]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]

OCA at 13:36, 17 July 2019

2019-07-17T13:36:29Z

←Older revision		Revision as of 13:36, 17 July 2019
Line 1:		Line 1:

	==THE STRUCTURE OF ALPHA-GALACTOSIDASE==		==THE STRUCTURE OF ALPHA-GALACTOSIDASE==
-	<StructureSection load='1szn' size='340' side='right' caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>	+	<StructureSection load='1szn' size='340' side='right'caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Atcc 13631]]		[[Category: Atcc 13631]]
		+	[[Category: Large Structures]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]
	[[Category: Golubev, A M]]		[[Category: Golubev, A M]]

OCA at 11:52, 12 October 2017

2017-10-12T11:52:08Z

←Older revision		Revision as of 11:52, 12 October 2017
Line 1:		Line 1:
		+
	==THE STRUCTURE OF ALPHA-GALACTOSIDASE==		==THE STRUCTURE OF ALPHA-GALACTOSIDASE==
	<StructureSection load='1szn' size='340' side='right' caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='1szn' size='340' side='right' caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trichoderma_reesei Trichoderma reesei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://pdbe.org/1szn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://pdbe.org/1szn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1szn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Galactosidase\|Galactosidase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
-	[[Category: ~~Trichoderma reesei~~]]	+	[[Category: Atcc 13631]]
	[[Category: Eneyskaya, E V]]		[[Category: Eneyskaya, E V]]
	[[Category: Golubev, A M]]		[[Category: Golubev, A M]]

OCA at 17:57, 11 September 2015

2015-09-11T17:57:04Z

←Older revision		Revision as of 17:57, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://pdbe.org/1szn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1szn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:23, 6 January 2015

2015-01-06T08:23:09Z

←Older revision		Revision as of 08:23, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Trichoderma reesei]]		[[Category: Trichoderma reesei]]
-	[[Category: Eneyskaya, E V.]]	+	[[Category: Eneyskaya, E V]]
-	[[Category: Golubev, A M.]]	+	[[Category: Golubev, A M]]
-	[[Category: Kulminskaya, A A.]]	+	[[Category: Kulminskaya, A A]]
-	[[Category: Nagem, R A.P.]]	+	[[Category: Nagem, R A.P]]
-	[[Category: Neto, J R.Brando.]]	+	[[Category: Neto, J R.Brando]]
-	[[Category: Neustroev, K N.]]	+	[[Category: Neustroev, K N]]
-	[[Category: Polikarpov, I.]]	+	[[Category: Polikarpov, I]]
-	[[Category: Shabalin, K A.]]	+	[[Category: Shabalin, K A]]
-	[[Category: Ev, A N.Savel.]]	+	[[Category: Ev, A N.Savel]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Two domain]]		[[Category: Two domain]]

OCA at 20:36, 28 September 2014

2014-09-28T20:36:10Z

←Older revision		Revision as of 20:36, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1szn.~~png\|left~~\|200px]]	+	==THE STRUCTURE OF ALPHA-GALACTOSIDASE==
		+	<StructureSection load='1szn' size='340' side='right' caption='[[1szn]], [[Resolution\|resolution]] 1.54Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szn RCSB], [http://www.ebi.ac.uk/pdbsum/1szn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.

-	~~{{STRUCTURE_1szn\| PDB=1szn \| SCENE= }}~~	+	Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.,Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043<ref>PMID:15136043</ref>

-	~~===THE STRUCTURE OF ALPHA-GALACTOSIDASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15136043}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1szn]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Trichoderma_reesei Trichoderma reesei]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA].~~	+

	==See Also==		==See Also==
	*[[Galactosidase\|Galactosidase]]		*[[Galactosidase\|Galactosidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015136043<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Trichoderma reesei]]		[[Category: Trichoderma reesei]]

OCA at 20:47, 20 October 2012

2012-10-20T20:47:35Z

←Older revision		Revision as of 20:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1szn]] is a 1 chain structure ~~of [[Galactosidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA].	+	[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA].

	==See Also==		==See Also==

OCA at 11:38, 27 July 2012

2012-07-27T11:38:03Z

←Older revision		Revision as of 11:38, 27 July 2012
Line 1:		Line 1:
	[[Image:1szn.png\|left\|200px]]		[[Image:1szn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1szn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1szn\| PDB=1szn \| SCENE= }}		{{STRUCTURE_1szn\| PDB=1szn \| SCENE= }}

	===THE STRUCTURE OF ALPHA-GALACTOSIDASE===		===THE STRUCTURE OF ALPHA-GALACTOSIDASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15136043}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15136043 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15136043}}		{{ABSTRACT_PUBMED_15136043}}

	==About this Structure==		==About this Structure==
-	[[1szn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Hypocrea_jecorina Hypocrea jecorina~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA].	+	[[1szn]] is a 1 chain structure of [[Galactosidase]] with sequence from [http://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZN OCA].
		+
		+	==See Also==
		+	*[[Galactosidase\|Galactosidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15136043~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015136043</ref><references group="xtra"/>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
-	[[Category: ~~Hypocrea jecorina~~]]	+	[[Category: Trichoderma reesei]]
	[[Category: Eneyskaya, E V.]]		[[Category: Eneyskaya, E V.]]
	[[Category: Golubev, A M.]]		[[Category: Golubev, A M.]]
Line 34:		Line 26:
	[[Category: Shabalin, K A.]]		[[Category: Shabalin, K A.]]
	[[Category: Ev, A N.Savel.]]		[[Category: Ev, A N.Savel.]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Hydrolase]]
		+	[[Category: Two domain]]

OCA: Protected "1szn" [edit=sysop:move=sysop]

2011-03-16T07:37:20Z

Protected "1szn" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:37, 16 March 2011