1t98 - Revision history

OCA at 08:38, 1 May 2024

2024-05-01T08:38:56Z

←Older revision		Revision as of 08:38, 1 May 2024
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	<StructureSection load='1t98' size='340' side='right'caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1t98' size='340' side='right'caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1T98 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [~~http~~://pdbe.org/1t98 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [https://pdbe.org/1t98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [https://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI]] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref>	+	[https://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t98 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t98 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.
-
-	The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins.,Fennell-Fezzie R, Gradia SD, Akey D, Berger JM EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272<ref>PMID:15902272</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1t98" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berger~~, J M~~]]	+	[[Category: Berger JM]]
-	[[Category: Fennell-Fezzie, R]]	+	[[Category: Fennell-Fezzie R]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Condensin]]~~	+
-	~~[[Category: Domain swapped]]~~	+
-	~~[[Category: Helix-turn helix]]~~	+
-	~~[[Category: Winged helix~~]]	+

OCA at 11:46, 24 December 2020

2020-12-24T11:46:34Z

←Older revision		Revision as of 11:46, 24 December 2020
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	==Crystal Structure of MukF(1-287)==		==Crystal Structure of MukF(1-287)==
-	<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='1t98' size='340' side='right'caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T98 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t9/1t98_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t9/1t98_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Fennell-Fezzie, R]]		[[Category: Fennell-Fezzie, R]]

OCA at 09:52, 12 October 2017

2017-10-12T09:52:28Z

←Older revision		Revision as of 09:52, 12 October 2017
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	<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>
	</table>		</table>
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Fennell-Fezzie, R]]		[[Category: Fennell-Fezzie, R]]

OCA at 18:40, 10 December 2016

2016-12-10T18:40:38Z

←Older revision		Revision as of 18:40, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal Structure of MukF(1-287)==		==Crystal Structure of MukF(1-287)==
	<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t98 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t98 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Fennell-Fezzie, R]]		[[Category: Fennell-Fezzie, R]]

OCA at 00:15, 12 September 2015

2015-09-12T00:15:40Z

←Older revision		Revision as of 00:15, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://pdbe.org/1t98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t98" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Berger, J M]]		[[Category: Berger, J M]]
	[[Category: Fennell-Fezzie, R]]		[[Category: Fennell-Fezzie, R]]

OCA at 21:16, 25 December 2014

2014-12-25T21:16:57Z

←Older revision		Revision as of 21:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI]] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Berger, J M.]]	+	[[Category: Berger, J M]]
-	[[Category: Fennell-Fezzie, R.]]	+	[[Category: Fennell-Fezzie, R]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Condensin]]		[[Category: Condensin]]

OCA at 20:42, 29 September 2014

2014-09-29T20:42:04Z

←Older revision		Revision as of 20:42, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1t98.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of MukF(1-287)==
		+	<StructureSection load='1t98' size='340' side='right' caption='[[1t98]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T98 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t98 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t98 RCSB], [http://www.ebi.ac.uk/pdbsum/1t98 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t9/1t98_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.

-	~~{{STRUCTURE_1t98\| PDB=1t98 \| SCENE= }}~~	+	The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins.,Fennell-Fezzie R, Gradia SD, Akey D, Berger JM EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272<ref>PMID:15902272</ref>

-	~~===Crystal Structure~~ of ~~MukF(1-287)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15902272}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1t98]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015902272<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Berger, J M.]]		[[Category: Berger, J M.]]

OCA: Protected "1t98" [edit=sysop:move=sysop]

2013-01-05T11:31:33Z

Protected "1t98" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:31, 5 January 2013

OCA at 11:31, 5 January 2013

2013-01-05T11:31:29Z

←Older revision		Revision as of 11:31, 5 January 2013
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-	{{Seed}}
	[[Image:1t98.png\|left\|200px]]		[[Image:1t98.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1t98\| PDB=1t98 \| SCENE= }}		{{STRUCTURE_1t98\| PDB=1t98 \| SCENE= }}

	===Crystal Structure of MukF(1-287)===		===Crystal Structure of MukF(1-287)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15902272}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15902272 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15902272}}		{{ABSTRACT_PUBMED_15902272}}

	==About this Structure==		==About this Structure==
-	~~1T98~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA].	+	[[1t98]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15902272~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015902272</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Berger, J M.]]		[[Category: Berger, J M.]]
	[[Category: Fennell-Fezzie, R.]]		[[Category: Fennell-Fezzie, R.]]
-	[[Category: ~~Coiled coil~~]]	+	[[Category: Cell cycle]]
	[[Category: Condensin]]		[[Category: Condensin]]
	[[Category: Domain swapped]]		[[Category: Domain swapped]]
	[[Category: Helix-turn helix]]		[[Category: Helix-turn helix]]
	[[Category: Winged helix]]		[[Category: Winged helix]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:52:27 2009''

OCA at 05:52, 18 February 2009

2009-02-18T05:52:28Z

←Older revision		Revision as of 05:52, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1T98 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA].	+	1T98 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA].

	==Reference==		==Reference==
-	The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15902272 15902272]~~	+	<ref group="xtra">PMID:15902272</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Berger, J M.]]		[[Category: Berger, J M.]]
	[[Category: Fennell-Fezzie, R.]]		[[Category: Fennell-Fezzie, R.]]
Line 34:		Line 33:
	[[Category: Winged helix]]		[[Category: Winged helix]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 14~~:19:~~35 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:52:27 2009''