1tgg - Revision history

OCA at 06:03, 5 July 2023

2023-07-05T06:03:45Z

←Older revision		Revision as of 06:03, 5 July 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=~~IIL~~:~~ISO-ISOLEUCINE~~'>~~IIL~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>~~[[1rh4\|1rh4]]~~</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [https://pdbe.org/1tgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [https://www.ebi.ac.uk/pdbsum/1tgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [https://pdbe.org/1tgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [https://www.ebi.ac.uk/pdbsum/1tgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgg ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alber, T]]	+	[[Category: Alber T]]
-	[[Category: Harbury~~, P B~~]]	+	[[Category: Harbury PB]]
-	[[Category: Kim~~, P S~~]]	+	[[Category: Kim PS]]
-	[[Category: Plecs~~, J J]]~~	+	[[Category: Plecs JJ]]
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: De novo design]]~~	+
-	~~[[Category: De novo protein~~]]	+

OCA at 09:27, 29 September 2021

2021-09-29T09:27:24Z

←Older revision		Revision as of 09:27, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1tgg' size='340' side='right'caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tgg' size='340' side='right'caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TGG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rh4\|1rh4]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [~~http~~://pdbe.org/1tgg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tgg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [https://pdbe.org/1tgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [https://www.ebi.ac.uk/pdbsum/1tgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:15, 18 December 2019

2019-12-18T14:15:20Z

←Older revision		Revision as of 14:15, 18 December 2019
Line 1:		Line 1:

	==RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER==		==RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER==
-	<StructureSection load='1tgg' size='340' side='right' caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1tgg' size='340' side='right'caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGG FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Harbury, P B]]		[[Category: Harbury, P B]]

OCA at 07:55, 14 March 2018

2018-03-14T07:55:22Z

←Older revision		Revision as of 07:55, 14 March 2018
Line 1:		Line 1:
		+
	==RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER==		==RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER==
	<StructureSection load='1tgg' size='340' side='right' caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tgg' size='340' side='right' caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [http://pdbe.org/1tgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [http://www.ebi.ac.uk/pdbsum/1tgg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [http://pdbe.org/1tgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [http://www.ebi.ac.uk/pdbsum/1tgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:02, 10 September 2015

2015-09-10T00:02:07Z

←Older revision		Revision as of 00:02, 10 September 2015
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [http://www.ebi.ac.uk/pdbsum/1tgg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [http://pdbe.org/1tgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [http://www.ebi.ac.uk/pdbsum/1tgg PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tgg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:20, 15 January 2015

2015-01-15T18:20:35Z

←Older revision		Revision as of 18:20, 15 January 2015
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alber, T.]]	+	[[Category: Alber, T]]
-	[[Category: Harbury, P B.]]	+	[[Category: Harbury, P B]]
-	[[Category: Kim, P S.]]	+	[[Category: Kim, P S]]
-	[[Category: Plecs, J J.]]	+	[[Category: Plecs, J J]]
	[[Category: Coiled coil]]		[[Category: Coiled coil]]
	[[Category: De novo design]]		[[Category: De novo design]]
	[[Category: De novo protein]]		[[Category: De novo protein]]

OCA at 08:22, 8 October 2014

2014-10-08T08:22:54Z

←Older revision		Revision as of 08:22, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1tgg.~~png~~\|~~left~~\|~~200px~~]]	+	==RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER==
		+	<StructureSection load='1tgg' size='340' side='right' caption='[[1tgg]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CGU:GAMMA-CARBOXY-GLUTAMIC+ACID'>CGU</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rh4\|1rh4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgg RCSB], [http://www.ebi.ac.uk/pdbsum/1tgg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Designing new protein folds requires a method for simultaneously optimizing the conformation of the backbone and the side-chains. One approach to this problem is the use of a parameterized backbone, which allows the systematic exploration of families of structures. We report the crystal structure of RH3, a right-handed, three-helix coiled coil that was designed using a parameterized backbone and detailed modeling of core packing. This crystal structure was determined using another rationally designed feature, a metal-binding site that permitted experimental phasing of the X-ray data. RH3 adopted the intended fold, which has not been observed previously in biological proteins. Unanticipated structural asymmetry in the trimer was a principal source of variation within the RH3 structure. The sequence of RH3 differs from that of a previously characterized right-handed tetramer, RH4, at only one position in each 11 amino acid sequence repeat. This close similarity indicates that the design method is sensitive to the core packing interactions that specify the protein structure. Comparison of the structures of RH3 and RH4 indicates that both steric overlap and cavity formation provide strong driving forces for oligomer specificity.

-	~~{{STRUCTURE_1tgg\| PDB=1tgg \| SCENE= }}~~	+	Structural test of the parameterized-backbone method for protein design.,Plecs JJ, Harbury PB, Kim PS, Alber T J Mol Biol. 2004 Sep 3;342(1):289-97. PMID:15313624<ref>PMID:15313624</ref>

-	~~===RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15313624}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA].~~	+	</StructureSection>
	[[Category: Alber, T.]]		[[Category: Alber, T.]]
	[[Category: Harbury, P B.]]		[[Category: Harbury, P B.]]

OCA: Protected "1tgg" [edit=sysop:move=sysop]

2013-01-05T13:39:42Z

Protected "1tgg" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:39, 5 January 2013

OCA at 13:39, 5 January 2013

2013-01-05T13:39:40Z

←Older revision		Revision as of 13:39, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1tgg.png\|left\|200px]]		[[Image:1tgg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tgg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tgg\| PDB=1tgg \| SCENE= }}		{{STRUCTURE_1tgg\| PDB=1tgg \| SCENE= }}

	===RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER===		===RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15313624}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15313624 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15313624}}		{{ABSTRACT_PUBMED_15313624}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA].	+	[[1tgg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGG OCA].
-		+
-	~~==Reference==~~	+
-	Structural test of the parameterized-backbone method for protein design., Plecs JJ, Harbury PB, Kim PS, Alber T, J Mol Biol. 2004 Sep 3;342(1):289-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313624 15313624]	+
	[[Category: Alber, T.]]		[[Category: Alber, T.]]
	[[Category: Harbury, P B.]]		[[Category: Harbury, P B.]]
Line 30:		Line 15:
	[[Category: Coiled coil]]		[[Category: Coiled coil]]
	[[Category: De novo design]]		[[Category: De novo design]]
-		+	[[Category: De novo protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Sun Jul 27 15:10:27 2008''~~	+

OCA at 12:10, 27 July 2008

2008-07-27T12:10:34Z

←Older revision		Revision as of 12:10, 27 July 2008
Line 1:		Line 1:
-	[[Image:1tgg.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1tgg.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1tgg\| PDB=1tgg \| SCENE= }}		{{STRUCTURE_1tgg\| PDB=1tgg \| SCENE= }}

-	~~'''~~RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER~~'''~~	+	===RH3 DESIGNED RIGHT-HANDED COILED COIL TRIMER===


-	~~==Overview==~~	+	<!--
-	~~Designing new protein folds requires a method for simultaneously optimizing the conformation of the backbone and the side-chains. One approach to~~ this ~~problem is the use of a parameterized backbone~~, ~~which allows the systematic exploration of families of structures. We report the crystal structure of RH3~~, a right-handed, three-helix coiled coil that was designed using a parameterized backbone and detailed modeling of core packing. This crystal structure was determined using another rationally designed feature, a metal-binding site that permitted experimental phasing of the ~~X-ray data. RH3 adopted~~ the ~~intended fold, which has not been observed previously in biological proteins~~. ~~Unanticipated structural asymmetry in the trimer was a principal source of variation within the RH3 structure~~. ~~The sequence of RH3 differs from that of a previously characterized right-handed tetramer~~, ~~RH4, at only one position in each 11 amino acid sequence repeat. This close similarity indicates that the design method~~ is ~~sensitive to~~ the core packing interactions that specify the protein structure. Comparison of the structures of RH3 and RH4 indicates that both steric overlap and cavity formation provide strong driving forces for oligomer specificity.	+	The line below this paragraph, {{ABSTRACT_PUBMED_15313624}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15313624 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15313624}}

	==About this Structure==		==About this Structure==
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	[[Category: Coiled coil]]		[[Category: Coiled coil]]
	[[Category: De novo design]]		[[Category: De novo design]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 09~~:55:23 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:10:27 2008''