1tgn - Revision history

OCA at 07:04, 27 September 2023

2023-09-27T07:04:38Z

←Older revision		Revision as of 07:04, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='1tgn' size='340' side='right'caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1tgn' size='340' side='right'caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [https://pdbe.org/1tgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [https://www.ebi.ac.uk/pdbsum/1tgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [https://pdbe.org/1tgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [https://www.ebi.ac.uk/pdbsum/1tgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kossiakoff~~, A A~~]]	+	[[Category: Kossiakoff AA]]
-	[[Category: Stroud~~, R M~~]]	+	[[Category: Stroud RM]]

OCA at 09:27, 29 September 2021

2021-09-29T09:27:26Z

←Older revision		Revision as of 09:27, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1tgn' size='340' side='right'caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1tgn' size='340' side='right'caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [~~http~~://pdbe.org/1tgn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tgn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgn ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [https://pdbe.org/1tgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [https://www.ebi.ac.uk/pdbsum/1tgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:39, 4 December 2019

2019-12-04T11:39:01Z

←Older revision		Revision as of 11:39, 4 December 2019
Line 1:		Line 1:

	==STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION==		==STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION==
-	<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='1tgn' size='340' side='right'caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tgn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tgn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1tgn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tgn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Kossiakoff, A A]]		[[Category: Kossiakoff, A A]]
	[[Category: Stroud, R M]]		[[Category: Stroud, R M]]

OCA at 06:58, 29 November 2017

2017-11-29T06:58:49Z

←Older revision		Revision as of 06:58, 29 November 2017
Line 1:		Line 1:
		+
	==STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION==		==STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION==
	<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://pdbe.org/1tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://pdbe.org/1tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1tgn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tgn" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:13, 9 February 2016

2016-02-09T19:13:11Z

←Older revision		Revision as of 19:13, 9 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tgn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:14, 11 September 2015

2015-09-11T01:14:42Z

←Older revision		Revision as of 01:14, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://pdbe.org/1tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tgn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: Kossiakoff, A A]]		[[Category: Kossiakoff, A A]]
	[[Category: Stroud, R M]]		[[Category: Stroud, R M]]

OCA at 08:16, 6 January 2015

2015-01-06T08:16:23Z

←Older revision		Revision as of 08:16, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Kossiakoff, A A.]]	+	[[Category: Kossiakoff, A A]]
-	[[Category: Stroud, R M.]]	+	[[Category: Stroud, R M]]

OCA at 21:57, 28 September 2014

2014-09-28T21:57:59Z

←Older revision		Revision as of 21:57, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1tgn.~~png\|left~~\|200px]]	+	==STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION==
		+	<StructureSection load='1tgn' size='340' side='right' caption='[[1tgn]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tgn RCSB], [http://www.ebi.ac.uk/pdbsum/1tgn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tgn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional crystal structure of bovine trypsinogen at approximately pH 7.5 was initially solved at 2.6 A resolution using the multiple isomorphous replacement method. Preliminary refinement cycles of the atomic coordinates trypsinogen have been carried out first to a resolution of 2.1 A, and later to 1.9 A, using constrained difference Fourier refinement; During the process, structure factors Fc and phi c were calculated from the trypsinogen structure and final interpretation was based on an electron-density map computed with terms (2 Fo - Fc) and phases phic at a resolution of 1.9 A. Crystals of trypsinogen grown from ethanol-water mixtures are trigonal with space group P3121, and cell dimension a = 55.17 A and c = 109.25 A. The structure is compared with the bovine diisopropylphosphoryltrypsin structure at approximately pH 7.2, oirginally determined from orthohombic crystals by Stroud et al. (Stroud, R.M., Kay L.M., and Dickerson, R.E. (1971), Cold Spring Harbor Symp. Quant. Biol. 36, 125-140; Stroud, R.M., Kay, L.M., and Dickerson, R.E. (1974), J. Mol. Biol. 83, 185-208), and later refined at 1.5 A resolution by Chambers and Stroud (Chambers, J.L., and Stroud, R.M. (1976), Acta Crystallogr. (in press)). At lower pH, 4.0-5.5 diogen, with cell dimensions a = 55.05 A and c = 109.45 A. This finding was used in the solution of the six trypsinogen heavy-atom derivatives prior to isomorphous phase analysis, and as a further basis of comparison between trypsinogen and the low pH trypsin structure. There are small differences between the two diisopropylphosphoryltrypsin structures. Bovine trypsinogen has a large and accessible cavity at the site where the native enzyme binds specific side chains of a substrate. The conformation and stability of the binding site differ from that found in trypsin at approximately pH 7.5, and from that in the low pH form of diisopropylphosphoryltrypsin. The catalytic site containing Asp-102, His-57, and Ser-195 is similar to that found in trypsin and contains a similar hydrogen-bounded network. The carboxyl group of Asp-194, which is salt bridged to the amino terminal of Ile-16 in native trypsin or other serine proteases, is apparently hydrogen bonded to internal solvent molecules in a loosely organized part of the zymogen structure. The unusually charged N-terminal hexapeptide of trypsinogen, whose removal leads to activation of the zymogen, lies on the outside surface of the molecule. There are significant structural changes which accompany activation in neighboring regions, which include residues 142-152, 215-550, 188A-195. The NH group of Gly-193, normally involved in stabilization of reaction intermediates (Steitz, T.A., Henderson, R., and Blow, D.M. (1969), J. Mol. Biol. 46, 337-348; Henderson, R. (1970), J. Mol. Biol. 54, 341-354; robertus, J.D., Kraut, J., Alden, R.A., and Birkoft, J.J. (1972), Biochemistry 11, 4293-4303) in the enzyme, is moved 1.9 A away from its position in trypsin...

-	~~{{STRUCTURE_1tgn\| PDB=1tgn \| SCENE= }}~~	+	Structure of bovine trypsinogen at 1.9 A resolution.,Kossiakoff AA, Chambers JL, Kay LM, Stroud RM Biochemistry. 1977 Feb 22;16(4):654-64. PMID:556951<ref>PMID:556951</ref>

-	~~===STRUCTURE OF BOVINE TRYPSINOGEN AT 1.9 ANGSTROMS RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_556951}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1tgn]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:000556951<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Kossiakoff, A A.]]		[[Category: Kossiakoff, A A.]]
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]

OCA at 14:51, 20 October 2012

2012-10-20T14:51:17Z

←Older revision		Revision as of 14:51, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1tgn]] is a 1 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA].	+	[[1tgn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGN OCA].

	==See Also==		==See Also==

OCA: Protected "1tgn" [edit=sysop:move=sysop]

2012-07-26T16:30:16Z

Protected "1tgn" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:30, 26 July 2012