1tin - Revision history

OCA at 08:41, 1 May 2024

2024-05-01T08:41:34Z

←Older revision		Revision as of 08:41, 1 May 2024
Line 1:		Line 1:

	==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==		==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==
-	<StructureSection load='1tin' size='340' side='right'caption='[[1tin~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1tin' size='340' side='right'caption='[[1tin]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Boston_marrow Boston marrow~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [https://pdbe.org/1tin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [https://www.ebi.ac.uk/pdbsum/1tin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tin ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [https://pdbe.org/1tin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [https://www.ebi.ac.uk/pdbsum/1tin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ITH5_CUCMA ITH5_CUCMA]] Specifically inhibits both trypsin and activated Hageman factor.	+	[https://www.uniprot.org/uniprot/ITH5_CUCMA ITH5_CUCMA] Specifically inhibits both trypsin and activated Hageman factor.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tin ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tin ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of Cucurbita maxima trypsin inhibitor-V (CMTI-V), which is also a specific inhibitor of the blood coagulation protein, factor XIIa, was determined by 1H NMR spectroscopy in combination with a distance-geometry and simulated annealing algorithm. Sequence-specific resonance assignments were made for all the main-chain and most of the side-chain hydrogens. Stereospecific assignments were also made for some of the beta-, gamma-, delta-, and epsilon-hydrogens and valine methyl hydrogens. The ring conformations of all six prolines in the inhibitor were determined on the basis of 1H-1H vicinal coupling constant patterns; most of the proline ring hydrogens were stereospecifically assigned on the basis of vicinal coupling constant and intraresidue nuclear Overhauser effect (NOE) patterns. Distance constraints were determined on the basis of NOEs between pairs of hydrogens. Dihedral angle constraints were determined from estimates of scalar coupling constants and intraresidue NOEs. On the basis of 727 interproton distance and 111 torsion angle constraints, which included backbone phi angles and side-chain chi 1, chi 2, chi 3, and chi 4 angles, 22 structures were calculated by a distance geometry algorithm and refined by energy minimization and simulated annealing methods. Both main-chain and side-chain atoms are well-defined, except for a loop region, two terminal residues, and some side-chain atoms located on the molecular surface. The average root mean squared deviation in the position for equivalent atoms between the 22 individual structures and the mean structure obtained by averaging their coordinates is 0.58 +/- 0.06 A for the main-chain atoms and 1.01 +/- 0.07 A for all the non-hydrogen atoms of residues 3-40 and 49-67. These structures were compared to the X-ray crystallographic structure of another protein of the same inhibitor family-chymotrypsin inhibitor-2 from barley seeds [CI-2; McPhalen, C. A., & James, M. N. G. (1987) Biochemistry 26, 261-269]. The main-chain folding patterns are highly similar for the two proteins, which possess 62% sequence differences. However, major differences are noted in the N- and C-terminal segments, which may be due to the presence of a disulfide bridge in CMTI-V, but not in CI-2.
-
-	Three-dimensional solution structure of Cucurbita maxima trypsin inhibitor-V determined by NMR spectroscopy.,Cai M, Gong Y, Kao JL, Krishnamoorthi R Biochemistry. 1995 Apr 18;34(15):5201-11. PMID:7711040<ref>PMID:7711040</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Trypsin inhibitor 3D structures\|Trypsin inhibitor 3D structures]]		*[[Trypsin inhibitor 3D structures\|Trypsin inhibitor 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Boston marrow~~]]	+	[[Category: Cucurbita maxima]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cai, M]]	+	[[Category: Cai M]]
-	[[Category: Gong, Y]]	+	[[Category: Gong Y]]
-	[[Category: Kao, J]]	+	[[Category: Kao J]]
-	[[Category: Krishnamoorthi, R]]	+	[[Category: Krishnamoorthi R]]
-	~~[[Category: Serine protease inhibitor~~]]	+

OCA at 09:28, 29 September 2021

2021-09-29T09:28:25Z

←Older revision		Revision as of 09:28, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1tin' size='340' side='right'caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1tin' size='340' side='right'caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [~~http~~://pdbe.org/1tin PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tin PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tin ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [https://pdbe.org/1tin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [https://www.ebi.ac.uk/pdbsum/1tin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ITH5_CUCMA ITH5_CUCMA]] Specifically inhibits both trypsin and activated Hageman factor.	+	[[https://www.uniprot.org/uniprot/ITH5_CUCMA ITH5_CUCMA]] Specifically inhibits both trypsin and activated Hageman factor.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Trypsin inhibitor\|Trypsin inhibitor]]	+	*[[Trypsin inhibitor 3D structures\|Trypsin inhibitor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:15, 4 December 2019

2019-12-04T11:15:47Z

←Older revision		Revision as of 11:15, 4 December 2019
Line 1:		Line 1:

	==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==		==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==
-	<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1tin' size='340' side='right'caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tin_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tin_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin inhibitor\|Trypsin inhibitor]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Boston marrow]]		[[Category: Boston marrow]]
		+	[[Category: Large Structures]]
	[[Category: Cai, M]]		[[Category: Cai, M]]
	[[Category: Gong, Y]]		[[Category: Gong, Y]]

OCA at 06:59, 29 November 2017

2017-11-29T06:59:02Z

←Older revision		Revision as of 06:59, 29 November 2017
Line 1:		Line 1:
		+
	==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==		==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==
	<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://pdbe.org/1tin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://pdbe.org/1tin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Trypsin inhibitor\|Trypsin inhibitor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:12, 8 February 2016

2016-02-08T02:12:35Z

←Older revision		Revision as of 02:12, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tin ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:53, 10 September 2015

2015-09-10T06:53:55Z

←Older revision		Revision as of 06:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cucurbita_maxima Cucurbita maxima~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Boston_marrow Boston marrow]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://pdbe.org/1tin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tin" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin inhibitor\|Trypsin inhibitor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cucurbita maxima~~]]	+	[[Category: Boston marrow]]
	[[Category: Cai, M]]		[[Category: Cai, M]]
	[[Category: Gong, Y]]		[[Category: Gong, Y]]

OCA at 19:49, 24 December 2014

2014-12-24T19:49:03Z

←Older revision		Revision as of 19:49, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ITH5_CUCMA ITH5_CUCMA]] Specifically inhibits both trypsin and activated Hageman factor.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Cucurbita maxima]]		[[Category: Cucurbita maxima]]
-	[[Category: Cai, M.]]	+	[[Category: Cai, M]]
-	[[Category: Gong, Y.]]	+	[[Category: Gong, Y]]
-	[[Category: Kao, J.]]	+	[[Category: Kao, J]]
-	[[Category: Krishnamoorthi, R.]]	+	[[Category: Krishnamoorthi, R]]
	[[Category: Serine protease inhibitor]]		[[Category: Serine protease inhibitor]]

OCA at 20:21, 29 September 2014

2014-09-29T20:21:41Z

←Older revision		Revision as of 20:21, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1tin.~~png\|left~~\|200px]]	+	==THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY==
		+	<StructureSection load='1tin' size='340' side='right' caption='[[1tin]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TIN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tin RCSB], [http://www.ebi.ac.uk/pdbsum/1tin PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tin_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of Cucurbita maxima trypsin inhibitor-V (CMTI-V), which is also a specific inhibitor of the blood coagulation protein, factor XIIa, was determined by 1H NMR spectroscopy in combination with a distance-geometry and simulated annealing algorithm. Sequence-specific resonance assignments were made for all the main-chain and most of the side-chain hydrogens. Stereospecific assignments were also made for some of the beta-, gamma-, delta-, and epsilon-hydrogens and valine methyl hydrogens. The ring conformations of all six prolines in the inhibitor were determined on the basis of 1H-1H vicinal coupling constant patterns; most of the proline ring hydrogens were stereospecifically assigned on the basis of vicinal coupling constant and intraresidue nuclear Overhauser effect (NOE) patterns. Distance constraints were determined on the basis of NOEs between pairs of hydrogens. Dihedral angle constraints were determined from estimates of scalar coupling constants and intraresidue NOEs. On the basis of 727 interproton distance and 111 torsion angle constraints, which included backbone phi angles and side-chain chi 1, chi 2, chi 3, and chi 4 angles, 22 structures were calculated by a distance geometry algorithm and refined by energy minimization and simulated annealing methods. Both main-chain and side-chain atoms are well-defined, except for a loop region, two terminal residues, and some side-chain atoms located on the molecular surface. The average root mean squared deviation in the position for equivalent atoms between the 22 individual structures and the mean structure obtained by averaging their coordinates is 0.58 +/- 0.06 A for the main-chain atoms and 1.01 +/- 0.07 A for all the non-hydrogen atoms of residues 3-40 and 49-67. These structures were compared to the X-ray crystallographic structure of another protein of the same inhibitor family-chymotrypsin inhibitor-2 from barley seeds [CI-2; McPhalen, C. A., & James, M. N. G. (1987) Biochemistry 26, 261-269]. The main-chain folding patterns are highly similar for the two proteins, which possess 62% sequence differences. However, major differences are noted in the N- and C-terminal segments, which may be due to the presence of a disulfide bridge in CMTI-V, but not in CI-2.

-	~~{{STRUCTURE_1tin\| PDB=1tin \| SCENE= }}~~	+	Three-dimensional solution structure of Cucurbita maxima trypsin inhibitor-V determined by NMR spectroscopy.,Cai M, Gong Y, Kao JL, Krishnamoorthi R Biochemistry. 1995 Apr 18;34(15):5201-11. PMID:7711040<ref>PMID:7711040</ref>

-	~~===THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7711040}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1tin]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cucurbita_maxima Cucurbita maxima]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007711040<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cucurbita maxima]]		[[Category: Cucurbita maxima]]
	[[Category: Cai, M.]]		[[Category: Cai, M.]]

OCA: Protected "1tin" [edit=sysop:move=sysop]

2013-01-05T11:01:29Z

Protected "1tin" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:01, 5 January 2013

OCA at 11:01, 5 January 2013

2013-01-05T11:01:24Z

←Older revision		Revision as of 11:01, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1tin.png\|left\|200px]]		[[Image:1tin.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tin", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tin\| PDB=1tin \| SCENE= }}		{{STRUCTURE_1tin\| PDB=1tin \| SCENE= }}

	===THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY===		===THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF CUCURBITA MAXIMA TRYPSIN INHIBITOR-V DETERMINED BY NMR SPECTROSCOPY===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_7711040}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 7711040 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_7711040}}		{{ABSTRACT_PUBMED_7711040}}

	==About this Structure==		==About this Structure==
-	~~1TIN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA].	+	[[1tin]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~7711040~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007711040</ref><references group="xtra"/>
	[[Category: Cucurbita maxima]]		[[Category: Cucurbita maxima]]
	[[Category: Cai, M.]]		[[Category: Cai, M.]]
Line 30:		Line 18:
	[[Category: Krishnamoorthi, R.]]		[[Category: Krishnamoorthi, R.]]
	[[Category: Serine protease inhibitor]]		[[Category: Serine protease inhibitor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:03:48 2009''