1tjw - Revision history

OCA at 06:31, 23 August 2023

2023-08-23T06:31:16Z

←Older revision		Revision as of 06:31, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1tjw' size='340' side='right'caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tjw' size='340' side='right'caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anapl Anapl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [https://pdbe.org/1tjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [https://www.ebi.ac.uk/pdbsum/1tjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [https://pdbe.org/1tjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [https://www.ebi.ac.uk/pdbsum/1tjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anapl]]~~	+	[[Category: Anas platyrhynchos]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Codding~~, P W~~]]	+	[[Category: Codding PW]]
-	[[Category: Horvatin, C]]	+	[[Category: Horvatin C]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: Lobsanov~~, Y D~~]]	+	[[Category: Lobsanov YD]]
-	[[Category: Sampaleanu~~, L M~~]]	+	[[Category: Sampaleanu LM]]
-	[[Category: Smith~~, G D~~]]	+	[[Category: Smith GD]]
-	[[Category: Tsai, M]]	+	[[Category: Tsai M]]
-	~~[[Category: Delta 2 crystallin]]~~	+
-	~~[[Category: Enzyme mechanism]]~~	+
-	~~[[Category: Eye lens protein]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 09:28, 29 September 2021

2021-09-29T09:28:47Z

←Older revision		Revision as of 09:28, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1tjw' size='340' side='right'caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tjw' size='340' side='right'caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [~~http~~://pdbe.org/1tjw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tjw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [https://pdbe.org/1tjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [https://www.ebi.ac.uk/pdbsum/1tjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:45, 25 December 2019

2019-12-25T13:45:07Z

←Older revision		Revision as of 13:45, 25 December 2019
Line 1:		Line 1:

	==Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate==		==Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate==
-	<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1tjw' size='340' side='right'caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1tjw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tjw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Crystallin 3D structures\|Crystallin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 39:
	[[Category: Anapl]]		[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
		+	[[Category: Large Structures]]
	[[Category: Codding, P W]]		[[Category: Codding, P W]]
	[[Category: Horvatin, C]]		[[Category: Horvatin, C]]

OCA at 08:36, 21 March 2018

2018-03-21T08:36:09Z

←Older revision		Revision as of 08:36, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate==		==Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate==
	<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://pdbe.org/1tjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://pdbe.org/1tjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tjw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tjw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:29, 8 February 2016

2016-02-08T16:29:43Z

←Older revision		Revision as of 16:29, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tjw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:24, 10 September 2015

2015-09-10T13:24:27Z

←Older revision		Revision as of 13:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anas_platyrhynchos Anas platyrhynchos~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://pdbe.org/1tjw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tjw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anas platyrhynchos~~]]	+	[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
	[[Category: Codding, P W]]		[[Category: Codding, P W]]

OCA at 12:34, 6 January 2015

2015-01-06T12:34:26Z

←Older revision		Revision as of 12:34, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
-	[[Category: Codding, P W.]]	+	[[Category: Codding, P W]]
-	[[Category: Horvatin, C.]]	+	[[Category: Horvatin, C]]
-	[[Category: Howell, P L.]]	+	[[Category: Howell, P L]]
-	[[Category: Lobsanov, Y D.]]	+	[[Category: Lobsanov, Y D]]
-	[[Category: Sampaleanu, L M.]]	+	[[Category: Sampaleanu, L M]]
-	[[Category: Smith, G D.]]	+	[[Category: Smith, G D]]
-	[[Category: Tsai, M~~.]]~~	+	[[Category: Tsai, M]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Delta 2 crystallin]]		[[Category: Delta 2 crystallin]]
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 20:25, 29 September 2014

2014-09-29T20:25:34Z

←Older revision		Revision as of 20:25, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1tjw.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate==
		+	<StructureSection load='1tjw' size='340' side='right' caption='[[1tjw]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AS1:ARGININOSUCCINATE'>AS1</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tjw RCSB], [http://www.ebi.ac.uk/pdbsum/1tjw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tjw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Delta crystallin, a taxon-specific crystallin present in avian eye lenses, is homologous to the urea cycle enzyme ASL (argininosuccinate lyase). Although there are two delta crystallin isoforms in duck lenses, ddeltac1 (duck delta1 crystallin) and ddeltac2 (duck delta2 crystallin), only ddeltac2 is catalytically active. Previous structural studies have suggested that residues Ser283 and His162 in the multi-subunit active site of ddeltac2/ASL are the putative catalytic acid/base, while the highly conserved, positively charged Lys289 is thought to help stabilize the carbanion intermediate. The strict conservation of a small hydroxy-containing residue (Thr or Ser) at position 161 adjacent to the putative catalytic base, as well as its proximity to the substrate in the S283A ddeltac2 enzyme-substrate complex, prompted us to investigate further the role this residue. Structures of the active T161S and inactive T161D ddeltac2 mutants, as well as T161D complexed with argininosuccinate, have been determined to 2.0 A resolution. The structures suggest that a hydroxy group is required at position 161 to help correctly position the side chain of Lys289 and the fumarate moiety of the substrate. Threonine is probably favoured over serine, because the interaction of its methyl group with Leu206 would restrict its conformational flexibility. Residues larger than Thr or Ser interfere with substrate binding, supporting previous suggestions that correct positioning of the substrate's fumarate moiety is essential for catalysis to occur. The presence of the 280s loop (i.e. a loop formed by residues 270-290) in the 'open' conformation suggests that loop closure, thought to be essential for sequestration of the substrate, may be triggered by the formation of the carbanion or aci-carboxylate intermediates, whose charge distribution more closely mimics that of the sulphate ion found in the active-site region of the inactive ddeltac1. The 280s loop in ddeltac1 is in the closed conformation.

-	~~{{STRUCTURE_1tjw\| PDB=1tjw \| SCENE= }}~~	+	Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.,Sampaleanu LM, Codding PW, Lobsanov YD, Tsai M, Smith GD, Horvatin C, Howell PL Biochem J. 2004 Dec 1;384(Pt 2):437-47. PMID:15320872<ref>PMID:15320872</ref>

-	~~===Crystal Structure~~ of ~~T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15320872}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1tjw]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Anas_platyrhynchos Anas platyrhynchos]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015320872<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]

OCA: Protected "1tjw" [edit=sysop:move=sysop]

2013-01-05T11:07:03Z

Protected "1tjw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:07, 5 January 2013

OCA at 11:07, 5 January 2013

2013-01-05T11:07:00Z

←Older revision		Revision as of 11:07, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1tjw.png\|left\|200px]]		[[Image:1tjw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tjw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tjw\| PDB=1tjw \| SCENE= }}		{{STRUCTURE_1tjw\| PDB=1tjw \| SCENE= }}

	===Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate===		===Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15320872}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15320872 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15320872}}		{{ABSTRACT_PUBMED_15320872}}

	==About this Structure==		==About this Structure==
-	~~1TJW~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA].	+	[[1tjw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15320872~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015320872</ref><references group="xtra"/>
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
Line 37:		Line 25:
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
-		+	[[Category: Lyase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 00:27:00 2009''~~	+