1u0a - Revision history

OCA at 06:35, 23 August 2023

2023-08-23T06:35:50Z

←Older revision		Revision as of 06:35, 23 August 2023
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	<StructureSection load='1u0a' size='340' side='right'caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='1u0a' size='340' side='right'caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus~~ macerans~~" (schardinger 1905) donker 1926~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA~~-~~D-GLUCOSE'>BGC</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2ayh\|2ayh]], [[1byh\|1byh]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [https://pdbe.org/1u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [https://www.ebi.ac.uk/pdbsum/1u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [https://pdbe.org/1u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [https://www.ebi.ac.uk/pdbsum/1u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GUB_PAEMA GUB_PAEMA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Licheninase~~]]	+	[[Category: Paenibacillus macerans]]
-	[[Category: Borriss, R]]	+	[[Category: Borriss R]]
-	[[Category: Gaiser~~, O J~~]]	+	[[Category: Gaiser OJ]]
-	[[Category: Heinemann, U]]	+	[[Category: Heinemann U]]
-	[[Category: Piotukh, K]]	+	[[Category: Piotukh K]]
-	[[Category: Planas, A]]	+	[[Category: Planas A]]
-	[[Category: Ponnuswamy~~, M N]]~~	+	[[Category: Ponnuswamy MN]]
-	~~[[Category: 3-1]]~~	+
-	~~[[Category: 4-beta-glucanase]]~~	+
-	~~[[Category: Active-site variant]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Jellyroll architecture]]~~	+
-	~~[[Category: Protein-carbohydrate interaction~~]]	+

OCA at 09:35, 29 September 2021

2021-09-29T09:35:02Z

←Older revision		Revision as of 09:35, 29 September 2021
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	<StructureSection load='1u0a' size='340' side='right'caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='1u0a' size='340' side='right'caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus macerans" (schardinger 1905) donker 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1U0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus macerans" (schardinger 1905) donker 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ayh\|2ayh]], [[1byh\|1byh]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [~~http~~://pdbe.org/1u0a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1u0a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [https://pdbe.org/1u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [https://www.ebi.ac.uk/pdbsum/1u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 13:57, 25 December 2019

2019-12-25T13:57:39Z

←Older revision		Revision as of 13:57, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide==		==Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide==
-	<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>	+	<StructureSection load='1u0a' size='340' side='right'caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus macerans" (schardinger 1905) donker 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus macerans" (schardinger 1905) donker 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Glucanase\|Glucanase]]	+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Licheninase]]		[[Category: Licheninase]]
	[[Category: Borriss, R]]		[[Category: Borriss, R]]

OCA at 08:47, 21 March 2018

2018-03-21T08:47:25Z

←Older revision		Revision as of 08:47, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide==		==Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide==
	<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://pdbe.org/1u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://pdbe.org/1u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:07, 7 February 2016

2016-02-07T21:07:30Z

←Older revision		Revision as of 21:07, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:59, 10 September 2015

2015-09-10T04:59:33Z

←Older revision		Revision as of 04:59, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Paenibacillus_macerans Paenibacillus~~ macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacillus_macerans"_(schardinger_1905)_donker_1926 "aerobacillus macerans" (schardinger 1905) donker 1926]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://pdbe.org/1u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1u0a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Licheninase]]		[[Category: Licheninase]]
-	[[Category: Paenibacillus macerans]]
	[[Category: Borriss, R]]		[[Category: Borriss, R]]
	[[Category: Gaiser, O J]]		[[Category: Gaiser, O J]]

OCA at 13:45, 6 January 2015

2015-01-06T13:45:39Z

←Older revision		Revision as of 13:45, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Licheninase]]		[[Category: Licheninase]]
	[[Category: Paenibacillus macerans]]		[[Category: Paenibacillus macerans]]
-	[[Category: Borriss, R.]]	+	[[Category: Borriss, R]]
-	[[Category: Gaiser, O J.]]	+	[[Category: Gaiser, O J]]
-	[[Category: Heinemann, U.]]	+	[[Category: Heinemann, U]]
-	[[Category: Piotukh, K.]]	+	[[Category: Piotukh, K]]
-	[[Category: Planas, A.]]	+	[[Category: Planas, A]]
-	[[Category: Ponnuswamy, M N.]]	+	[[Category: Ponnuswamy, M N]]
	[[Category: 3-1]]		[[Category: 3-1]]
	[[Category: 4-beta-glucanase]]		[[Category: 4-beta-glucanase]]

OCA at 20:24, 28 September 2014

2014-09-28T20:24:39Z

←Older revision		Revision as of 20:24, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1u0a.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide==
		+	<StructureSection load='1u0a' size='340' side='right' caption='[[1u0a]], [[Resolution\|resolution]] 1.64Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ayh\|2ayh]], [[1byh\|1byh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0a RCSB], [http://www.ebi.ac.uk/pdbsum/1u0a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Depolymerization of polysaccharides is catalyzed by highly specific enzymes that promote hydrolysis of the scissile glycosidic bond by an activated water molecule. 1,3-1,4-beta-Glucanases selectively cleave beta-1,4 glycosidic bonds in 3-O-substituted glucopyranosyl units within polysaccharides with mixed linkage. The reaction follows a double-displacement mechanism by which the configuration of the anomeric C(1)-atom of the glucosyl unit in subsite -I is retained. Here we report the high-resolution crystal structure of the hybrid 1,3-1,4-beta-glucanase H(A16-M)(E105Q/E109Q) in complex with a beta-glucan tetrasaccharide. The structure shows four beta-d-glucosyl moieties bound to the substrate-binding cleft covering subsites -IV to -I, thus corresponding to the reaction product. The ten active-site residues Asn26, Glu63, Arg65, Phe92, Tyr94, Glu105, Asp107, Glu109, Asn182 and Trp184 form a network of hydrogen bonds and hydrophobic stacking interactions with the substrate. These residues were previously identified by mutational analysis as significant for stabilization of the enzyme-carbohydrate complex, with Glu105 and Glu109 being the catalytic residues. Compared to the Michaelis complex model, the tetrasaccharide moiety is slightly shifted toward that part of the cleft binding the non-reducing end of the substrate, but shows previously unanticipated strong stacking interactions with Phe92 in subsite -I. A number of specific hydrogen-bond contacts between the enzyme and the equatorial O(2), O(3) and O(6) hydroxyl groups of the glucosyl residues in subsites -I, -II and -III are the structural basis for the observed substrate specificity of 1,3-1,4-beta-glucanases. Kinetic analysis of enzyme variants with the all beta-1,3 linked polysaccharide laminarin identified key residues mediating substrate specificity in good agreement with the structural data. The comparison with structures of the apo-enzyme H(A16-M) and a covalent enzyme-inhibitor (E.I) complex, together with kinetic and mutagenesis data, yields new insights into the structural requirements for substrate binding and catalysis. A detailed view of enzyme-carbohydrate interactions is presented and mechanistic implications are discussed.

-	~~{{STRUCTURE_1u0a\| PDB=1u0a \| SCENE= }}~~	+	Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanase.,Gaiser OJ, Piotukh K, Ponnuswamy MN, Planas A, Borriss R, Heinemann U J Mol Biol. 2006 Apr 7;357(4):1211-25. Epub 2006 Jan 25. PMID:16483609<ref>PMID:16483609</ref>

-	~~===Crystal structure~~ of the ~~engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16483609}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1u0a]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Paenibacillus_macerans Paenibacillus macerans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA].~~	+

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016483609<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Licheninase]]		[[Category: Licheninase]]
	[[Category: Paenibacillus macerans]]		[[Category: Paenibacillus macerans]]

OCA at 07:52, 20 October 2012

2012-10-20T07:52:08Z

←Older revision		Revision as of 07:52, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1u0a]] is a 4 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA].	+	[[1u0a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0A OCA].

	==See Also==		==See Also==

OCA: Protected "1u0a" [edit=sysop:move=sysop]

2012-07-25T17:43:07Z

Protected "1u0a" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:43, 25 July 2012