1u15 - Revision history

OCA at 06:36, 23 August 2023

2023-08-23T06:36:13Z

←Older revision		Revision as of 06:36, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1u15' size='340' side='right'caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1u15' size='340' side='right'caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anapl Anapl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1hy0\|1hy0]]~~, [[~~1hy1~~\|~~1hy1~~]]~~, [[1u16\|1u16]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.~~1 4.3.2.1] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [https://pdbe.org/1u15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [https://www.ebi.ac.uk/pdbsum/1u15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u15 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [https://pdbe.org/1u15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [https://www.ebi.ac.uk/pdbsum/1u15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARLY1_ANAPL ARLY1_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. Despite possessing the necessary catalytic residues, this protein does not function as an enzymatically active argininosuccinate lyase.<ref>PMID:7944404</ref>	+	[https://www.uniprot.org/uniprot/ARLY1_ANAPL ARLY1_ANAPL] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. Despite possessing the necessary catalytic residues, this protein does not function as an enzymatically active argininosuccinate lyase.<ref>PMID:7944404</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anapl]]~~	+	[[Category: Anas platyrhynchos]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Creagh, L]]	+	[[Category: Creagh L]]
-	[[Category: Greene, C]]	+	[[Category: Greene C]]
-	[[Category: Haynes, C]]	+	[[Category: Haynes C]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: Sampaleanu~~, L M~~]]	+	[[Category: Sampaleanu LM]]
-	[[Category: Tsai, M]]	+	[[Category: Tsai M]]
-	~~[[Category: Duck-delta-crystallin]]~~	+
-	~~[[Category: Enzyme mechanism]]~~	+
-	~~[[Category: Eye lens protein]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 09:35, 29 September 2021

2021-09-29T09:35:17Z

←Older revision		Revision as of 09:35, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1u15' size='340' side='right'caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1u15' size='340' side='right'caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1U15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [~~http~~://pdbe.org/1u15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1u15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1u15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [https://pdbe.org/1u15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [https://www.ebi.ac.uk/pdbsum/1u15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARLY1_ANAPL ARLY1_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. Despite possessing the necessary catalytic residues, this protein does not function as an enzymatically active argininosuccinate lyase.<ref>PMID:7944404</ref>	+	[[https://www.uniprot.org/uniprot/ARLY1_ANAPL ARLY1_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. Despite possessing the necessary catalytic residues, this protein does not function as an enzymatically active argininosuccinate lyase.<ref>PMID:7944404</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:36, 25 December 2019

2019-12-25T13:36:08Z

←Older revision		Revision as of 13:36, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)==		==Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)==
-	<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1u15' size='340' side='right'caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1u15" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1u15" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Crystallin 3D structures\|Crystallin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Anapl]]		[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
		+	[[Category: Large Structures]]
	[[Category: Creagh, L]]		[[Category: Creagh, L]]
	[[Category: Greene, C]]		[[Category: Greene, C]]

OCA at 08:27, 21 March 2018

2018-03-21T08:27:48Z

←Older revision		Revision as of 08:27, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)==		==Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)==
	<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://pdbe.org/1u15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://pdbe.org/1u15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u1/1u15_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u1/1u15_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:39, 9 February 2016

2016-02-09T05:39:59Z

←Older revision		Revision as of 05:39, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u15 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:10, 10 September 2015

2015-09-10T19:10:04Z

←Older revision		Revision as of 19:10, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anas_platyrhynchos Anas platyrhynchos~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://pdbe.org/1u15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARLY1_ANAPL ARLY1_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. Despite possessing the necessary catalytic residues, this protein does not function as an enzymatically active argininosuccinate lyase.<ref>PMID:7944404</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1u15" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anas platyrhynchos~~]]	+	[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
	[[Category: Creagh, L]]		[[Category: Creagh, L]]

OCA at 12:22, 6 January 2015

2015-01-06T12:22:34Z

←Older revision		Revision as of 12:22, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
-	[[Category: Creagh, L.]]	+	[[Category: Creagh, L]]
-	[[Category: Greene, C.]]	+	[[Category: Greene, C]]
-	[[Category: Haynes, C.]]	+	[[Category: Haynes, C]]
-	[[Category: Howell, P L.]]	+	[[Category: Howell, P L]]
-	[[Category: Sampaleanu, L M.]]	+	[[Category: Sampaleanu, L M]]
-	[[Category: Tsai, M~~.]]~~	+	[[Category: Tsai, M]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Duck-delta-crystallin]]		[[Category: Duck-delta-crystallin]]
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 20:20, 29 September 2014

2014-09-29T20:20:31Z

←Older revision		Revision as of 20:20, 29 September 2014
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-	[[~~Image:~~1u15.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)==
		+	<StructureSection load='1u15' size='340' side='right' caption='[[1u15]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U15 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy0\|1hy0]], [[1hy1\|1hy1]], [[1u16\|1u16]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u15 RCSB], [http://www.ebi.ac.uk/pdbsum/1u15 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u1/1u15_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Delta-crystallin is directly related to argininosuccinate lyase (ASL), and catalyzes the reversible hydrolysis of argininosuccinate to arginine and fumarate. Two delta-crystallin isoforms exist in duck lenses, delta1 and delta2, which are 94% identical in amino acid sequence. Although the sequences of duck delta2-crystallin (ddeltac2) and duck delta1-crystallin (ddeltac1) are 69 and 71% identical to that of human ASL, respectively, only ddeltac2 has maintained ASL activity. Domain exchange experiments and comparisons of various delta-crystallin structures have suggested that the amino acid substitutions in the 20's (residues 22-31) and 70's (residues 74-89) loops of ddeltac1 are responsible for the loss of enzyme activity in this isoform. To test this hypothesis, a double loop mutant (DLM) of ddeltac1 was constructed in which all the residues that differ between the two isoforms in the 20's and 70's loops were mutated to those of ddeltac2. Contrary to expectations, kinetic analysis of the DLM found that it was enzymatically inactive. Furthermore, binding of argininosuccinate by the DLM, as well as the ddeltac1, could not be detected by isothermal titration calorimetry (ITC). To examine the conformation of the 20's and 70's loops in the DLM, and to understand why the DLM is unable to bind the substrate, its structure was determined to 2.5 A resolution. Comparison of this structure with both wild-type ddeltac1 and ddeltac2 structures reveals that the conformations of the 20's and 70's loops in the DLM mutant are very similar to those of ddeltac2. This suggests that the five amino acid substitutions in domain 1 which lie outside of the two loop regions and which are different in the DLM, and ddeltac2, must be important enzymatically. The structure of the DLM in complex with sulfate was also determined to 2.2 A resolution. This structure demonstrates that the conformational changes of the 280's loop and domain 3, previously observed in ddeltac1, also occur in the DLM upon sulfate binding, reinforcing the hypothesis that these events may occur in the active ddeltac2 protein during catalysis.

-	~~{{STRUCTURE_1u15\| PDB=1u15 \| SCENE= }}~~	+	A duck delta1 crystallin double loop mutant provides insight into residues important for argininosuccinate lyase activity.,Tsai M, Sampaleanu LM, Greene C, Creagh L, Haynes C, Howell PL Biochemistry. 2004 Sep 21;43(37):11672-82. PMID:15362851<ref>PMID:15362851</ref>

-	~~===Crystal structure~~ of ~~a duck-delta-crystallin-1 double loop mutant (DLM)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15362851}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1u15]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Anas_platyrhynchos Anas platyrhynchos]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015362851<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]

OCA: Protected "1u15" [edit=sysop:move=sysop]

2013-01-05T10:59:23Z

Protected "1u15" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 5 January 2013

OCA at 10:59, 5 January 2013

2013-01-05T10:59:21Z

←Older revision		Revision as of 10:59, 5 January 2013
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-	{{Seed}}
	[[Image:1u15.png\|left\|200px]]		[[Image:1u15.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1u15", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1u15\| PDB=1u15 \| SCENE= }}		{{STRUCTURE_1u15\| PDB=1u15 \| SCENE= }}

	===Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)===		===Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15362851}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15362851 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15362851}}		{{ABSTRACT_PUBMED_15362851}}

	==About this Structure==		==About this Structure==
-	~~1U15~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA].	+	[[1u15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U15 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15362851~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015362851</ref><references group="xtra"/>
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
Line 36:		Line 24:
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
-		+	[[Category: Lyase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 06:54:50 2009''~~	+