1ucg - Revision history

OCA at 07:33, 25 October 2023

2023-10-25T07:33:54Z

←Older revision		Revision as of 07:33, 25 October 2023
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	<StructureSection load='1ucg' size='340' side='right'caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1ucg' size='340' side='right'caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Balsam_pear Balsam pear~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Ribonuclease_T~~(2) ~~Ribonuclease T(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [https://pdbe.org/1ucg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [https://www.ebi.ac.uk/pdbsum/1ucg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [https://pdbe.org/1ucg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [https://www.ebi.ac.uk/pdbsum/1ucg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNMC1_MOMCH RNMC1_MOMCH] Ribonuclease cleaving preferentially the 5'-side of uridine.<ref>PMID:10964705</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Balsam pear]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kimura, M]]	+	[[Category: Momordica charantia]]
-	[[Category: Numata, T]]	+	[[Category: Kimura M]]
-	[[Category: Suzuki, A]]	+	[[Category: Numata T]]
-	[[Category: Tanaka, I]]	+	[[Category: Suzuki A]]
-	[[Category: Yao, M]]	+	[[Category: Tanaka I]]
-	~~[[Category: Alpha plus beta]]~~	+	[[Category: Yao M]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:40, 29 September 2021

2021-09-29T09:40:03Z

←Older revision		Revision as of 09:40, 29 September 2021
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	<StructureSection load='1ucg' size='340' side='right'caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1ucg' size='340' side='right'caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [~~http~~://pdbe.org/1ucg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ucg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [https://pdbe.org/1ucg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [https://www.ebi.ac.uk/pdbsum/1ucg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:43, 25 December 2019

2019-12-25T13:43:46Z

←Older revision		Revision as of 13:43, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of Ribonuclease MC1 N71T mutant==		==Crystal structure of Ribonuclease MC1 N71T mutant==
-	<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='1ucg' size='340' side='right'caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Balsam pear]]		[[Category: Balsam pear]]
		+	[[Category: Large Structures]]
	[[Category: Kimura, M]]		[[Category: Kimura, M]]
	[[Category: Numata, T]]		[[Category: Numata, T]]

OCA at 08:34, 21 March 2018

2018-03-21T08:34:43Z

←Older revision		Revision as of 08:34, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of Ribonuclease MC1 N71T mutant==		==Crystal structure of Ribonuclease MC1 N71T mutant==
	<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://pdbe.org/1ucg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://pdbe.org/1ucg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ucg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:27, 11 September 2015

2015-09-11T14:27:02Z

←Older revision		Revision as of 14:27, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Momordica_charantia Momordica charantia~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://pdbe.org/1ucg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ucg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Momordica charantia~~]]	+	[[Category: Balsam pear]]
	[[Category: Kimura, M]]		[[Category: Kimura, M]]
	[[Category: Numata, T]]		[[Category: Numata, T]]

OCA at 13:32, 6 January 2015

2015-01-06T13:32:14Z

←Older revision		Revision as of 13:32, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
-	[[Category: Kimura, M.]]	+	[[Category: Kimura, M]]
-	[[Category: Numata, T.]]	+	[[Category: Numata, T]]
-	[[Category: Suzuki, A.]]	+	[[Category: Suzuki, A]]
-	[[Category: Tanaka, I.]]	+	[[Category: Tanaka, I]]
-	[[Category: Yao, M.]]	+	[[Category: Yao, M]]
	[[Category: Alpha plus beta]]		[[Category: Alpha plus beta]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 22:47, 28 September 2014

2014-09-28T22:47:08Z

←Older revision		Revision as of 22:47, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ucg.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Ribonuclease MC1 N71T mutant==
		+	<StructureSection load='1ucg' size='340' side='right' caption='[[1ucg]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]], [[1ucc\|1ucc]], [[1ucd\|1ucd]], [[1j1f\|1j1f]], [[1j1g\|1j1g]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucg RCSB], [http://www.ebi.ac.uk/pdbsum/1ucg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribonuclease MC1 (RNase MC1), isolated from bitter gourd seeds, is a uridine specific RNase belonging to the RNase T2 family. Mutations of Asn71 in RNase MC1 to the amino acids Thr (N71T) and Ser (N71S) in guanosine preferential RNases altered the substrate specificity from uridine specific to guanosine specific, as shown by the transphosphorylation of diribonucleoside monophosphates [Numata, T., et al. (2001) Biochemistry 40, 524-530]. To elucidate the structural basis for the alteration of substrate specificity, crystal structures of the RNase MC1 mutants N71T and N71S, free or complexed with 5'-GMP, were determined at resolutions higher than 2 A. In the N71T-5'-GMP and N71S-5'-GMP complexes, the guanine moiety was, as in the case of the uracil moiety bound to wild-type RNase MC1, firmly stabilized in the B2 site by an extensive network of hydrogen bonds and hydrophobic interactions. Structure comparisons showed that mutations of Asn71 to Thr or Ser cause an enlargement of the B2 site, which then make it feasible to insert a guanine base into the B2 site of mutants N71T and N71S. This binding further allows for hydrogen bonding interaction of the side chain hydroxyl groups of Thr71 or Ser71 with the N7 atom of the guanine base. The mode of guanine binding of mutants N71T and N71S was found to be essentially identical to that of a guanosine preferential RNase NW from Nicotiana glutinosa. In particular, hydrogen bonds between the N7 atom of the guanine base and the hydroxyl groups of the amino acids at position 71 (RNase MC1 numbering) were completely conserved in three guanosine preferential enzymes, thereby indicating that the hydrogen bond may play an essential role in guanine binding in guanosine preferential RNases in the RNase T2 family. Consequently, it can be concluded that amino acids at position 71 (RNase MC1 numbering) serve as one of the determinants for substrate specificity (or preference) in the RNase T2 fimily by changing the size and shape of the B2 site.

-	~~{{STRUCTURE_1ucg\| PDB=1ucg \| SCENE= }}~~	+	Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity.,Numata T, Suzuki A, Kakuta Y, Kimura K, Yao M, Tanaka I, Yoshida Y, Ueda T, Kimura M Biochemistry. 2003 May 13;42(18):5270-8. PMID:12731868<ref>PMID:12731868</ref>

-	~~===Crystal structure~~ of ~~Ribonuclease MC1 N71T mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12731868}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ucg]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Momordica_charantia Momordica charantia]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012731868<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: Kimura, M.]]		[[Category: Kimura, M.]]

OCA at 19:34, 20 October 2012

2012-10-20T19:34:57Z

←Older revision		Revision as of 19:34, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ucg]] is a 2 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA].	+	[[1ucg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA].

	==See Also==		==See Also==

OCA: Protected "1ucg" [edit=sysop:move=sysop]

2012-07-27T07:49:33Z

Protected "1ucg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:49, 27 July 2012

OCA at 07:49, 27 July 2012

2012-07-27T07:49:31Z

←Older revision		Revision as of 07:49, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ucg.png\|left\|200px]]		[[Image:1ucg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ucg", creates the "Structure Box" on the page.
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1ucg\| PDB=1ucg \| SCENE= }}		{{STRUCTURE_1ucg\| PDB=1ucg \| SCENE= }}

	===Crystal structure of Ribonuclease MC1 N71T mutant===		===Crystal structure of Ribonuclease MC1 N71T mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12731868}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12731868 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12731868}}		{{ABSTRACT_PUBMED_12731868}}

	==About this Structure==		==About this Structure==
-	~~1UCG~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA].	+	[[1ucg]] is a 2 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCG OCA].
		+
		+	==See Also==
		+	*[[Ribonuclease\|Ribonuclease]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12731868~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012731868</ref><references group="xtra"/>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: Kimura, M.]]		[[Category: Kimura, M.]]
Line 31:		Line 22:
	[[Category: Yao, M.]]		[[Category: Yao, M.]]
	[[Category: Alpha plus beta]]		[[Category: Alpha plus beta]]
-		+	[[Category: Hydrolase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:32:05 2009''~~	+