1uhk - Revision history

OCA at 23:53, 27 December 2023

2023-12-27T23:53:39Z

←Older revision		Revision as of 23:53, 27 December 2023
Line 3:		Line 3:
	<StructureSection load='1uhk' size='340' side='right'caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1uhk' size='340' side='right'caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [https://pdbe.org/1uhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [https://www.ebi.ac.uk/pdbsum/1uhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [https://pdbe.org/1uhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [https://www.ebi.ac.uk/pdbsum/1uhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AEQ2_AEQVI AEQ2_AEQVI]] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.	+	[https://www.uniprot.org/uniprot/AEQ2_AEQVI AEQ2_AEQVI] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chong~~, K T~~]]	+	[[Category: Chong KT]]
-	[[Category: Inouye, S]]	+	[[Category: Inouye S]]
-	[[Category: Nakagawa, A]]	+	[[Category: Nakagawa A]]
-	[[Category: Shimomura, O]]	+	[[Category: Shimomura O]]
-	[[Category: Teranishi, K]]	+	[[Category: Teranishi K]]
-	[[Category: Toma, S]]	+	[[Category: Toma S]]
-	~~[[Category: Ef-hand motif]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Luminescent protein~~]]	+

OCA at 06:10, 6 October 2021

2021-10-06T06:10:54Z

←Older revision		Revision as of 06:10, 6 October 2021
Line 3:		Line 3:
	<StructureSection load='1uhk' size='340' side='right'caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1uhk' size='340' side='right'caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [~~http~~://pdbe.org/1uhk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1uhk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [https://pdbe.org/1uhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [https://www.ebi.ac.uk/pdbsum/1uhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AEQ2_AEQVI AEQ2_AEQVI]] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.	+	[[https://www.uniprot.org/uniprot/AEQ2_AEQVI AEQ2_AEQVI]] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:53, 1 January 2020

2020-01-01T12:53:58Z

←Older revision		Revision as of 12:53, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of n-aequorin==		==Crystal structure of n-aequorin==
-	<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1uhk' size='340' side='right'caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Aeqvi]]		[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Chong, K T]]		[[Category: Chong, K T]]
	[[Category: Inouye, S]]		[[Category: Inouye, S]]

OCA at 07:43, 28 March 2018

2018-03-28T07:43:41Z

←Older revision		Revision as of 07:43, 28 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of n-aequorin==		==Crystal structure of n-aequorin==
	<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://pdbe.org/1uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://pdbe.org/1uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uh/1uhk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uh/1uhk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uhk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:18, 11 September 2015

2015-09-11T11:18:59Z

←Older revision		Revision as of 11:18, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aequorea_victoria Aequorea victoria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://pdbe.org/1uhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1uhk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aequorea victoria~~]]	+	[[Category: Aeqvi]]
	[[Category: Chong, K T]]		[[Category: Chong, K T]]
	[[Category: Inouye, S]]		[[Category: Inouye, S]]

OCA at 15:44, 25 December 2014

2014-12-25T15:44:34Z

←Older revision		Revision as of 15:44, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AEQ2_AEQVI AEQ2_AEQVI]] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
-	[[Category: Chong, K T.]]	+	[[Category: Chong, K T]]
-	[[Category: Inouye, S.]]	+	[[Category: Inouye, S]]
-	[[Category: Nakagawa, A.]]	+	[[Category: Nakagawa, A]]
-	[[Category: Shimomura, O.]]	+	[[Category: Shimomura, O]]
-	[[Category: Teranishi, K.]]	+	[[Category: Teranishi, K]]
-	[[Category: Toma, S.]]	+	[[Category: Toma, S]]
-	[[Category: ~~4 ef~~-hand motif]]	+	[[Category: Ef-hand motif]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]

OCA at 18:58, 29 September 2014

2014-09-29T18:58:25Z

←Older revision		Revision as of 18:58, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1uhk.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of n-aequorin==
		+	<StructureSection load='1uhk' size='340' side='right' caption='[[1uhk]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UHK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZN:(2S,8R)-8-BENZYL-2-HYDROPEROXY-6-(4-HYDROXYPHENYL)-2-(2-NAPHTHYLMETHYL)-7,8-DIHYDROIMIDAZO[1,2-A]PYRAZIN-3(2H)-ONE'>CZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ej3\|1ej3]], [[1el4\|1el4]], [[1jf2\|1jf2]], [[1uhh\|1uhh]], [[1uhi\|1uhi]], [[1uhj\|1uhj]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uhk RCSB], [http://www.ebi.ac.uk/pdbsum/1uhk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uh/1uhk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The photoprotein aequorin emits light by an intramolecular reaction in the presence of a trace amount of Ca(2+). Semi-synthetic aequorins, produced by replacing the coelenterazine moiety in aequorin with the analogues of coelenterazine, show widely different sensitivities to Ca(2+). To understand the structural basis of the Ca(2+)-sensitivity, we determined the crystal structures of four semi-synthetic aequorins (cp-, i-, br- and n-aequorins) at resolutions of 1.6-1.8 A. In general, the protein structures of these semi-synthetic aequorins are almost identical to native aequorin. Of the four EF-hand domains in the molecule, EF-hand II does not bind Ca(2+), and the loop of EF-hand IV is clearly deformed. It is most likely that the binding of Ca(2+) with EF-hands I and III triggers luminescence. Although little difference was found in the overall structures of aequorins investigated, some significant differences were found in the interactions between the substituents of coelenterazine moiety and the amino acid residues in the binding pocket. The coelenterazine moieties in i-, br-, and n-aequorins have bulky 2-substitutions, which can interfere with the conformational changes of protein structure that follow the binding of Ca(2+) to aequorin. In cp-aequorin, the cyclopentylmethyl group that substitutes for the original 8-benzyl group does not interact hydrophobically with the protein part, giving the coelenterazine moiety more conformational freedom to promote the light-emitting reaction. The differences of various semi-synthetic aequorins in Ca(2+)-sensitivity and reaction rate are explained by the capability of the involved groups and structures to undergo conformational changes in response to the Ca(2+)-binding.

-	~~{{STRUCTURE_1uhk\| PDB=1uhk \| SCENE= }}~~	+	The crystal structures of semi-synthetic aequorins.,Toma S, Chong KT, Nakagawa A, Teranishi K, Inouye S, Shimomura O Protein Sci. 2005 Feb;14(2):409-16. Epub 2005 Jan 4. PMID:15632284<ref>PMID:15632284</ref>

-	~~===Crystal structure~~ of ~~n-aequorin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15632284}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1uhk]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aequorea_victoria Aequorea victoria]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015632284<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
	[[Category: Chong, K T.]]		[[Category: Chong, K T.]]

OCA: Protected "1uhk" [edit=sysop:move=sysop]

2013-01-05T11:48:21Z

Protected "1uhk" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:48, 5 January 2013

OCA at 11:48, 5 January 2013

2013-01-05T11:48:17Z

←Older revision		Revision as of 11:48, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1uhk.png\|left\|200px]]		[[Image:1uhk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1uhk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1uhk\| PDB=1uhk \| SCENE= }}		{{STRUCTURE_1uhk\| PDB=1uhk \| SCENE= }}

	===Crystal structure of n-aequorin===		===Crystal structure of n-aequorin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15632284}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15632284 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15632284}}		{{ABSTRACT_PUBMED_15632284}}

	==About this Structure==		==About this Structure==
-	~~1UHK~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA].	+	[[1uhk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15632284~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015632284</ref><references group="xtra"/>
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
	[[Category: Chong, K T.]]		[[Category: Chong, K T.]]
Line 33:		Line 21:
	[[Category: 4 ef-hand motif]]		[[Category: 4 ef-hand motif]]
	[[Category: Complex]]		[[Category: Complex]]
-		+	[[Category: Luminescent protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 20:48:21 2009''~~	+

OCA at 18:48, 17 February 2009

2009-02-17T18:48:24Z

←Older revision		Revision as of 18:48, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1UHK is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA].	+	1UHK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHK OCA].

	==Reference==		==Reference==
-	~~The crystal structures of semi-synthetic aequorins., Toma S, Chong KT, Nakagawa A, Teranishi K, Inouye S, Shimomura O, Protein Sci. 2005 Feb;14(2):409-16. Epub 2005 Jan 4.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15632284 15632284]~~	+	<ref group="xtra">PMID:15632284</ref><references group="xtra"/>
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
-	[[Category: Single protein]]
	[[Category: Chong, K T.]]		[[Category: Chong, K T.]]
	[[Category: Inouye, S.]]		[[Category: Inouye, S.]]
Line 35:		Line 34:
	[[Category: Complex]]		[[Category: Complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:45:~~55 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:48:21 2009''