1uu6 - Revision history

OCA at 13:20, 9 May 2024

2024-05-09T13:20:51Z

←Older revision		Revision as of 13:20, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichocladium_griseum Trichocladium griseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA~~-~~D-GLUCOSE'>BGC</scene>~~, ~~<scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~PCA~~:~~PYROGLUTAMIC+ACID~~'>~~PCA~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>~~[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [https://pdbe.org/1uu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uu6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [https://pdbe.org/1uu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uu6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8NJY3_9PEZI Q8NJY3_9PEZI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellulase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berglund~~, G I~~]]	+	[[Category: Trichocladium griseum]]
-	[[Category: Driguez~~, T H~~]]	+	[[Category: Berglund GI]]
-	[[Category: Kenne, L]]	+	[[Category: Driguez TH]]
-	[[Category: Mitchinson, C]]	+	[[Category: Kenne L]]
-	[[Category: Sandgren, M]]	+	[[Category: Mitchinson C]]
-	[[Category: Shaw, A]]	+	[[Category: Sandgren M]]
-	[[Category: Stahlberg, J]]	+	[[Category: Shaw A]]
-	~~[[Category: Cellulose degradation]]~~	+	[[Category: Stahlberg J]]
-	~~[[Category: Endoglucanase]]~~	+
-	~~[[Category: Gh family 12]]~~	+
-	~~[[Category: Glycosyl hydrolase]]~~	+
-	~~[[Category: Humicola grisea cel12a]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ligand complex~~]]	+

OCA at 17:14, 15 December 2021

2021-12-15T17:14:12Z

←Older revision		Revision as of 17:14, 15 December 2021
Line 3:		Line 3:
	<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [~~http~~://pdbe.org/1uu6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1uu6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1uu6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [https://pdbe.org/1uu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uu6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:38, 11 March 2020

2020-03-11T07:38:13Z

←Older revision		Revision as of 07:38, 11 March 2020
Line 3:		Line 3:
	<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Cellulase]]		[[Category: Cellulase]]
-	[[Category: Humicola grisea]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Berglund, G I]]		[[Category: Berglund, G I]]

OCA at 13:11, 1 January 2020

2020-01-01T13:11:23Z

←Older revision		Revision as of 13:11, 1 January 2020
Line 1:		Line 1:

	==X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE==		==X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE==
-	<StructureSection load='1uu6' size='340' side='right' caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='1uu6' size='340' side='right'caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Glucanase\|Glucanase]]	+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Humicola grisea]]		[[Category: Humicola grisea]]
		+	[[Category: Large Structures]]
	[[Category: Berglund, G I]]		[[Category: Berglund, G I]]
	[[Category: Driguez, T H]]		[[Category: Driguez, T H]]

OCA at 08:01, 28 March 2018

2018-03-28T08:01:17Z

←Older revision		Revision as of 08:01, 28 March 2018
Line 1:		Line 1:
		+
	==X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE==		==X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE==
	<StructureSection load='1uu6' size='340' side='right' caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1uu6' size='340' side='right' caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://pdbe.org/1uu6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://pdbe.org/1uu6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uu6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uu6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uu6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:49, 9 February 2016

2016-02-09T20:49:48Z

←Older revision		Revision as of 20:49, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uu6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:54, 11 September 2015

2015-09-11T01:54:40Z

←Older revision		Revision as of 01:54, 11 September 2015
Line 7:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://pdbe.org/1uu6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1uu6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:53, 6 January 2015

2015-01-06T12:53:10Z

←Older revision		Revision as of 12:53, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Humicola grisea]]		[[Category: Humicola grisea]]
-	[[Category: Berglund, G I.]]	+	[[Category: Berglund, G I]]
-	[[Category: Driguez, T H.]]	+	[[Category: Driguez, T H]]
-	[[Category: Kenne, L.]]	+	[[Category: Kenne, L]]
-	[[Category: Mitchinson, C.]]	+	[[Category: Mitchinson, C]]
-	[[Category: Sandgren, M.]]	+	[[Category: Sandgren, M]]
-	[[Category: Shaw, A.]]	+	[[Category: Shaw, A]]
-	[[Category: Stahlberg, J~~.]]~~	+	[[Category: Stahlberg, J]]
-	~~[[Category: Cellulase~~]]	+
	[[Category: Cellulose degradation]]		[[Category: Cellulose degradation]]
	[[Category: Endoglucanase]]		[[Category: Endoglucanase]]

OCA at 22:28, 28 September 2014

2014-09-28T22:28:17Z

←Older revision		Revision as of 22:28, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1uu6.~~png~~\|~~left~~\|200px]]	+	==X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE==
		+	<StructureSection load='1uu6' size='340' side='right' caption='[[1uu6]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UU6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1olr\|1olr]], [[1uu4\|1uu4]], [[1uu5\|1uu5]], [[1w2u\|1w2u]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uu6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uu6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uu6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uu6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As part of an ongoing enzyme discovery program to investigate the properties and catalytic mechanism of glycoside hydrolase family 12 (GH 12) endoglucanases, a GH family that contains several cellulases that are of interest in industrial applications, we have solved four new crystal structures of wild-type Humicola grisea Cel12A in complexes formed by soaking with cellobiose, cellotetraose, cellopentaose, and a thio-linked cellotetraose derivative (G2SG2). These complex structures allow mapping of the non-covalent interactions between the enzyme and the glucosyl chain bound in subsites -4 to +2 of the enzyme, and shed light on the mechanism and function of GH 12 cellulases. The unhydrolysed cellopentaose and the G2SG2 cello-oligomers span the active site of the catalytically active H.grisea Cel12A enzyme, with the pyranoside bound in subsite -1 displaying a S31 skew boat conformation. After soaking in cellotetraose, the cello-oligomer that is found bound in site -4 to -1 contains a beta-1,3-linkage between the two cellobiose units in the oligomer, which is believed to have been formed by a transglycosylation reaction that has occurred during the ligand soak of the protein crystals. The close fit of this ligand and the binding sites occupied suggest a novel mixed beta-glucanase activity for this enzyme.

-	~~{{STRUCTURE_1uu6\| PDB=1uu6 \| SCENE= }}~~	+	Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A.,Sandgren M, Berglund GI, Shaw A, Stahlberg J, Kenne L, Desmet T, Mitchinson C J Mol Biol. 2004 Oct 1;342(5):1505-17. PMID:15364577<ref>PMID:15364577</ref>

-	~~===X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH A SOAKED CELLOPENTAOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15364577}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1uu6]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Humicola_grisea Humicola grisea]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA].~~	+

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015364577<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Humicola grisea]]		[[Category: Humicola grisea]]

OCA at 15:05, 20 October 2012

2012-10-20T15:05:56Z

←Older revision		Revision as of 15:05, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1uu6]] is a 1 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA].	+	[[1uu6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UU6 OCA].

	==See Also==		==See Also==