1v9h - Revision history

OCA at 07:50, 25 October 2023

2023-10-25T07:50:23Z

←Older revision		Revision as of 07:50, 25 October 2023
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	<StructureSection load='1v9h' size='340' side='right'caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1v9h' size='340' side='right'caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Balsam_pear Balsam pear~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [https://pdbe.org/1v9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [https://www.ebi.ac.uk/pdbsum/1v9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [https://pdbe.org/1v9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [https://www.ebi.ac.uk/pdbsum/1v9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9h ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNMC1_MOMCH RNMC1_MOMCH] Ribonuclease cleaving preferentially the 5'-side of uridine.<ref>PMID:10964705</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Balsam pear]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kakuta, Y]]	+	[[Category: Momordica charantia]]
-	[[Category: Kimura, K]]	+	[[Category: Kakuta Y]]
-	[[Category: Kimura, M]]	+	[[Category: Kimura K]]
-	[[Category: Numata, T]]	+	[[Category: Kimura M]]
-	~~[[Category: Hydolase]]~~	+	[[Category: Numata T]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nucleic acid]]~~	+
-	~~[[Category: Rna~~]]	+

OCA at 06:28, 6 October 2021

2021-10-06T06:28:18Z

←Older revision		Revision as of 06:28, 6 October 2021
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	<StructureSection load='1v9h' size='340' side='right'caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1v9h' size='340' side='right'caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1V9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [~~http~~://pdbe.org/1v9h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1v9h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [https://pdbe.org/1v9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [https://www.ebi.ac.uk/pdbsum/1v9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9h ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:52, 1 January 2020

2020-01-01T12:52:55Z

←Older revision		Revision as of 12:52, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP==		==Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP==
-	<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1v9h' size='340' side='right'caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>		<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
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	</StructureSection>		</StructureSection>
	[[Category: Balsam pear]]		[[Category: Balsam pear]]
		+	[[Category: Large Structures]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Kimura, K]]		[[Category: Kimura, K]]

OCA at 07:42, 28 March 2018

2018-03-28T07:42:17Z

←Older revision		Revision as of 07:42, 28 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP==		==Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP==
	<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://pdbe.org/1v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://pdbe.org/1v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9h ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/1v9h_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/1v9h_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:10, 8 February 2016

2016-02-08T18:10:26Z

←Older revision		Revision as of 18:10, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v9h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:06, 10 September 2015

2015-09-10T14:06:56Z

←Older revision		Revision as of 14:06, 10 September 2015
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	<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Momordica_charantia Momordica charantia~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Balsam_pear Balsam pear]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://pdbe.org/1v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1v9h" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Momordica charantia~~]]	+	[[Category: Balsam pear]]
	[[Category: Kakuta, Y]]		[[Category: Kakuta, Y]]
	[[Category: Kimura, K]]		[[Category: Kimura, K]]

OCA at 13:30, 6 January 2015

2015-01-06T13:30:47Z

←Older revision		Revision as of 13:30, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>		<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
-	[[Category: Kakuta, Y.]]	+	[[Category: Kakuta, Y]]
-	[[Category: Kimura, K.]]	+	[[Category: Kimura, K]]
-	[[Category: Kimura, M.]]	+	[[Category: Kimura, M]]
-	[[Category: Numata, T.]]	+	[[Category: Numata, T]]
	[[Category: Hydolase]]		[[Category: Hydolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Nucleic acid]]		[[Category: Nucleic acid]]
	[[Category: Rna]]		[[Category: Rna]]

OCA at 22:46, 28 September 2014

2014-09-28T22:46:27Z

←Older revision		Revision as of 22:46, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1v9h.~~png\|left~~\|200px]]	+	==Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP==
		+	<StructureSection load='1v9h' size='340' side='right' caption='[[1v9h]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V9H FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v9h RCSB], [http://www.ebi.ac.uk/pdbsum/1v9h PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/1v9h_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ribonuclease MC1 (RNase MC1) from the seeds of the bitter gourd belongs to the RNase T2 family. We evaluated the contribution of 11 amino acids conserved in the RNase T2 family to protein folding of RNase MC1. Thermal unfolding experiments showed that substitution of Tyr(101), Phe(102), Ala(105), and Phe(190) resulted in a significant decrease in themostability; the T(m) values were 47-58 degrees C compared to that for the wild type (64 degrees C). Mutations of Pro(125), Gly(127), Gly(144), and Val(165) caused a moderate decrease in thermostability (T(m): 60-62 degrees C). In contrast, mutations of Asp(107) and Gly(173) did little effect on thermostability. The contribution of Tyr(101), Phe(102), Pro(125), and Gly(127) to protein stability was further corroborated by means of Gdn-HCl unfolding and protease digestions. Taken together, it appeared that Tyr(101), Phe(102), Ala(105), Pro(125), Gly(127), Gly(144), Leu(162), Val(165), and Phe(190) conserved in the RNase T2 family play an important role in the stability of the proteins.

-	~~{{STRUCTURE_1v9h\| PDB=1v9h \| SCENE= }}~~	+	Amino acids conserved at the C-terminal half of the ribonuclease T2 family contribute to protein stability of the enzymes.,Kimura K, Numata T, Kakuta Y, Kimura M Biosci Biotechnol Biochem. 2004 Aug;68(8):1748-57. PMID:15322360<ref>PMID:15322360</ref>

-	~~===Crystal structure~~ of the ~~RNase MC1 mutant Y101A in complex with 5'-UMP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15322360}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1v9h]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Momordica_charantia Momordica charantia]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015322360<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: Kakuta, Y.]]		[[Category: Kakuta, Y.]]

OCA at 11:01, 20 October 2012

2012-10-20T11:01:04Z

←Older revision		Revision as of 11:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1v9h]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA].	+	[[1v9h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9H OCA].

	==See Also==		==See Also==

OCA: Protected "1v9h" [edit=sysop:move=sysop]

2012-07-26T03:59:22Z

Protected "1v9h" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:59, 26 July 2012