1vxq - Revision history

OCA: Redirecting to 4lt8

2014-12-10T15:06:44Z

Redirecting to 4lt8

←Older revision		Revision as of 15:06, 10 December 2014
Line 1:		Line 1:
-	~~==Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome==~~	+	#REDIRECT [[4lt8]] This PDB entry is obsolete and replaced by 4lt8
-	~~<StructureSection load='1vxq' size='340' side='right' caption='~~[[~~1vxq]], [[Resolution\|resolution]] 3.14Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[1vxq~~]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VXQ FirstGlance]. <br>	+
-	~~</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vxp\|1vxp]], [[1vxs\|1vxs]], [[1vxt\|1vxt]]</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vxq RCSB], [http://www.ebi.ac.uk/pdbsum/1vxq PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6~~ and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by ~~the ribosome in maintaining the correct translational reading frame.~~	+
-		+
-	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Thermus thermophilus]]~~	+
-	~~[[Category: Dunham, C M.]]~~	+
-	~~[[Category: Dunkle, J A.]]~~	+
-	~~[[Category: Maehigashi, T.]]~~	+
-	~~[[Category: 16]]~~	+
-	~~[[Category: 23]]~~	+
-	~~[[Category: 30]]~~	+
-	~~[[Category: 70]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Ribosomal subunit]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Transfer rna]]~~	+
-	~~[[Category: Trna]]~~	+

OCA at 03:04, 4 September 2014

2014-09-04T03:04:58Z

←Older revision		Revision as of 03:04, 4 September 2014
Line 8:		Line 8:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vxq RCSB], [http://www.ebi.ac.uk/pdbsum/1vxq PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vxq RCSB], [http://www.ebi.ac.uk/pdbsum/1vxq PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
		+
		+	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA: Protected "1vxq" [edit=sysop:move=sysop]

2014-08-07T02:34:51Z

Protected "1vxq" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:34, 7 August 2014

OCA: New page: ==Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome== == Structural highlights ==
<table><tr><td colspan='2'>[[1vxq]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VXQ OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VXQ FirstGlance]. 
</td></tr><tr><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> 
<tr><td class="sblockLbl">[[Non-Standard_Residue|NonStd Res:]]</td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>
<tr><td class="sblockLbl">[[Related_structure|Related:]]</td><td class="sblockDat">[[1vxp|1vxp]], [[1vxs|1vxs]], [[1vxt|1vxt]]</td></tr>
<tr><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vxq RCSB], [http://www.ebi.ac.uk/pdbsum/1vxq PDBsum]</td></tr>
<table>
TOC
</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Dunham, C M.]]
[[Category: Dunkle, J A.]]
[[Category: Maehigashi, T.]]
[[Category: 16]]
[[Category: 23]]
[[Category: 30]]
[[Category: 70]]
[[Category: Protein biosynthesis]]
[[Category: Ribosomal subunit]]
[[Category: Ribosome]]
[[Category: Ribosome]]
[[Category: Rna]]
[[Category: Transfer rna]]
[[Category: Trna]]