1w2v - Revision history

OCA at 13:13, 13 December 2023

2023-12-13T13:13:33Z

←Older revision		Revision as of 13:13, 13 December 2023
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	<StructureSection load='1w2v' size='340' side='right'caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1w2v' size='340' side='right'caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1W2V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W2V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Endo-~~1,4-~~beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [https://pdbe.org/1w2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [https://www.ebi.ac.uk/pdbsum/1w2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w2v ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [~~http~~://pdbe.org/1w2v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1w2v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1w2v ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/XYNA_CELJU XYNA_CELJU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellvibrio ~~cellulosa nagy et al. 2002]]~~	+	[[Category: Cellvibrio japonicus]]
-	~~[[Category: Endo-1,4-beta-xylanase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andrews, S]]	+	[[Category: Andrews S]]
-	[[Category: Davies~~, G J~~]]	+	[[Category: Davies GJ]]
-	[[Category: Ducros~~, V M.A~~]]	+	[[Category: Ducros VMA]]
-	[[Category: Glbert~~, H J~~]]	+	[[Category: Glbert HJ]]
-	[[Category: Lakey~~, J H~~]]	+	[[Category: Lakey JH]]
-	[[Category: Pell~~, G N~~]]	+	[[Category: Pell GN]]
-	[[Category: Taylor~~, E J~~]]	+	[[Category: Taylor EJ]]
-	[[Category: Vincent, F]]	+	[[Category: Vincent F]]
-	~~[[Category: Calcium ion]]~~	+
-	~~[[Category: Error prone pcr]]~~	+
-	~~[[Category: Family 10]]~~	+
-	~~[[Category: Glycosyle hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Thermostable]]~~	+
-	~~[[Category: Xylanase~~]]	+

OCA at 11:49, 29 July 2020

2020-07-29T11:49:54Z

←Older revision		Revision as of 11:49, 29 July 2020
Line 1:		Line 1:

	==The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus==		==The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus==
-	<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='1w2v' size='340' side='right'caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1W2V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1W2V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://pdbe.org/1w2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w2v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://pdbe.org/1w2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w2v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	[[Category: Cellvibrio cellulosa nagy et al. 2002]]		[[Category: Cellvibrio cellulosa nagy et al. 2002]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Andrews, S]]		[[Category: Andrews, S]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]

OCA at 07:13, 4 April 2018

2018-04-04T07:13:54Z

←Older revision		Revision as of 07:13, 4 April 2018
Line 1:		Line 1:
-	==~~THE~~ 3-~~DIMENSIONAL STRUCTURE OF A THERMOSTABLE MUTANT OF A XYLANASE~~ (~~XYN10A~~) ~~FROM CELLVIBRIO JAPONICUS~~==	+
		+	==The 3-dimensional structure of a thermostable mutant of a xylanase (Xyn10A) from Cellvibrio japonicus==
	<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://pdbe.org/1w2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://pdbe.org/1w2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w2v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/1w2v_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/1w2v_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:26, 10 February 2016

2016-02-10T07:26:50Z

←Older revision		Revision as of 07:26, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w2v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:41, 11 September 2015

2015-09-11T06:41:08Z

←Older revision		Revision as of 06:41, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cellvibrio_japonicus Cellvibrio japonicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W2V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W2V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://pdbe.org/1w2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1w2v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellvibrio ~~japonicus~~]]	+	[[Category: Cellvibrio cellulosa nagy et al. 2002]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Andrews, S]]		[[Category: Andrews, S]]

OCA at 13:21, 6 January 2015

2015-01-06T13:21:51Z

←Older revision		Revision as of 13:21, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W2V FirstGlance]. <br>		<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W2V FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
-	[[Category: Andrews, S.]]	+	[[Category: Andrews, S]]
-	[[Category: Davies, G J.]]	+	[[Category: Davies, G J]]
-	[[Category: Ducros, V M.A.]]	+	[[Category: Ducros, V M.A]]
-	[[Category: Glbert, H J.]]	+	[[Category: Glbert, H J]]
-	[[Category: Lakey, J H.]]	+	[[Category: Lakey, J H]]
-	[[Category: Pell, G N.]]	+	[[Category: Pell, G N]]
-	[[Category: Taylor, E J.]]	+	[[Category: Taylor, E J]]
-	[[Category: Vincent, F.]]	+	[[Category: Vincent, F]]
	[[Category: Calcium ion]]		[[Category: Calcium ion]]
	[[Category: Error prone pcr]]		[[Category: Error prone pcr]]

OCA at 19:50, 29 September 2014

2014-09-29T19:50:43Z

←Older revision		Revision as of 19:50, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1w2v.~~png~~\|~~left~~\|200px]]	+	==THE 3-DIMENSIONAL STRUCTURE OF A THERMOSTABLE MUTANT OF A XYLANASE (XYN10A) FROM CELLVIBRIO JAPONICUS==
		+	<StructureSection load='1w2v' size='340' side='right' caption='[[1w2v]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W2V FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1clx\|1clx]], [[1e5n\|1e5n]], [[1e8r\|1e8r]], [[1qld\|1qld]], [[1w2p\|1w2p]], [[1w32\|1w32]], [[1w3h\|1w3h]], [[1xys\|1xys]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w2v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w2v RCSB], [http://www.ebi.ac.uk/pdbsum/1w2v PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/1w2v_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metal ions such as calcium often play a key role in protein thermostability. The inclusion of metal ions in industrial processes is, however, problematic. Thus, the evolution of enzymes that display enhanced stability, which is not reliant on divalent metals, is an important biotechnological goal. Here we have used forced protein evolution to interrogate whether the stabilizing effect of calcium in an industrially relevant enzyme can be replaced with amino acid substitutions. Our study has focused on the GH10 xylanase CjXyn10A from Cellvibrio japonicus, which contains an extended calcium binding loop that confers proteinase resistance and thermostability. Three rounds of error-prone PCR and selection identified a treble mutant, D262N/A80T/R347C, which in the absence of calcium is more thermostable than wild type CjXyn10A bound to the divalent metal. D262N influences the properties of the calcium binding site, A80T fills a cavity in the enzyme, increasing the number of hydrogen bonds and van der Waals interactions, and the R347C mutation introduces a disulfide bond that decreases the free energy of the unfolded enzyme. A derivative of CjXyn10A (CfCjXyn10A) in which the calcium binding loop has been replaced with a much shorter loop from Cellulomonas fimi CfXyn10A was also subjected to forced protein evolution to select for thermostablizing mutations. Two amino acid substitutions within the introduced loop and the A80T mutation increased the thermostability of the enzyme. This study demonstrates how forced protein evolution can be used to introduce enhanced stability into industrially relevant enzymes while removing calcium as a major stability determinant.

-	~~{{STRUCTURE_1w2v\| PDB=1w2v \| SCENE= }}~~	+	The use of forced protein evolution to investigate and improve stability of family 10 xylanases. The production of Ca2+-independent stable xylanases.,Andrews SR, Taylor EJ, Pell G, Vincent F, Ducros VM, Davies GJ, Lakey JH, Gilbert HJ J Biol Chem. 2004 Dec 24;279(52):54369-79. Epub 2004 Sep 27. PMID:15452124<ref>PMID:15452124</ref>

-	~~===THE 3-DIMENSIONAL STRUCTURE OF A THERMOSTABLE MUTANT OF A XYLANASE (XYN10A) FROM CELLVIBRIO JAPONICUS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15452124}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1w2v]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015452124<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]

OCA: Protected "1w2v" [edit=sysop:move=sysop]

2013-01-05T13:11:50Z

Protected "1w2v" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:11, 5 January 2013

OCA at 13:11, 5 January 2013

2013-01-05T13:11:48Z

←Older revision		Revision as of 13:11, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1w2v.png\|left\|200px]]		[[Image:1w2v.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1w2v", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1w2v\| PDB=1w2v \| SCENE= }}		{{STRUCTURE_1w2v\| PDB=1w2v \| SCENE= }}

	===THE 3-DIMENSIONAL STRUCTURE OF A THERMOSTABLE MUTANT OF A XYLANASE (XYN10A) FROM CELLVIBRIO JAPONICUS===		===THE 3-DIMENSIONAL STRUCTURE OF A THERMOSTABLE MUTANT OF A XYLANASE (XYN10A) FROM CELLVIBRIO JAPONICUS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15452124}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15452124 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15452124}}		{{ABSTRACT_PUBMED_15452124}}

	==About this Structure==		==About this Structure==
-	~~1W2V~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA].	+	[[1w2v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15452124~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015452124</ref><references group="xtra"/>
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
Line 42:		Line 30:
	[[Category: Thermostable]]		[[Category: Thermostable]]
	[[Category: Xylanase]]		[[Category: Xylanase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:29:35 2009''

OCA at 14:29, 17 February 2009

2009-02-17T14:29:36Z

←Older revision		Revision as of 14:29, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1W2V is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA].	+	1W2V is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W2V OCA].

	==Reference==		==Reference==
-	The use of forced protein evolution to investigate and improve stability of family 10 xylanases. The production of Ca2+-independent stable xylanases., Andrews SR, Taylor EJ, Pell G, Vincent F, Ducros VM, Davies GJ, Lakey JH, Gilbert HJ, J Biol Chem. 2004 Dec 24;279(52):54369-79. Epub 2004 Sep 27. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15452124 15452124]~~	+	<ref group="xtra">PMID:15452124</ref><references group="xtra"/>
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
-	[[Category: Single protein]]
	[[Category: Andrews, S.]]		[[Category: Andrews, S.]]
	[[Category: Davies, G J.]]		[[Category: Davies, G J.]]
Line 44:		Line 43:
	[[Category: Xylanase]]		[[Category: Xylanase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 23~~:10:~~14 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:29:35 2009''