1wo9 - Revision history

OCA at 09:28, 6 December 2023

2023-12-06T09:28:32Z

←Older revision		Revision as of 09:28, 6 December 2023
Line 1:		Line 1:

	==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==		==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==
-	<StructureSection load='1wo9' size='340' side='right'caption='[[1wo9~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1wo9' size='340' side='right'caption='[[1wo9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Locusta_migratoria_migratorioides Locusta migratoria migratorioides]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [https://pdbe.org/1wo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [https://www.ebi.ac.uk/pdbsum/1wo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wo9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [https://pdbe.org/1wo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [https://www.ebi.ac.uk/pdbsum/1wo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wo9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8WQ22_LOCMI Q8WQ22_LOCMI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Darbon, H]]	+	[[Category: Locusta migratoria migratorioides]]
-	[[Category: Ferrat, G]]	+	[[Category: Darbon H]]
-	[[Category: Kellenberger, C]]	+	[[Category: Ferrat G]]
-	[[Category: Leone, P]]	+	[[Category: Kellenberger C]]
-	[[Category: Roussel, A]]	+	[[Category: Leone P]]
-	~~[[Category: Hydrolase inhibitor~~]]	+	[[Category: Roussel A]]

OCA at 16:23, 27 October 2021

2021-10-27T16:23:20Z

←Older revision		Revision as of 16:23, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1wo9' size='340' side='right'caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1wo9' size='340' side='right'caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [~~http~~://pdbe.org/1wo9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wo9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wo9 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [https://pdbe.org/1wo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [https://www.ebi.ac.uk/pdbsum/1wo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wo9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:45, 22 January 2020

2020-01-22T17:45:04Z

←Older revision		Revision as of 17:45, 22 January 2020
Line 1:		Line 1:

	==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==		==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==
-	<StructureSection load='1wo9' size='340' side='right' caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1wo9' size='340' side='right'caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Darbon, H]]		[[Category: Darbon, H]]
	[[Category: Ferrat, G]]		[[Category: Ferrat, G]]

OCA at 16:28, 11 April 2018

2018-04-11T16:28:13Z

←Older revision		Revision as of 16:28, 11 April 2018
Line 1:		Line 1:
		+
	==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==		==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==
	<StructureSection load='1wo9' size='340' side='right' caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1wo9' size='340' side='right' caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [http://pdbe.org/1wo9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1wo9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [http://pdbe.org/1wo9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1wo9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wo9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:12, 11 September 2015

2015-09-11T01:12:51Z

←Older revision		Revision as of 01:12, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1wo9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [http://pdbe.org/1wo9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1wo9 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wo9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:15, 15 January 2015

2015-01-15T18:15:51Z

←Older revision		Revision as of 18:15, 15 January 2015
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Darbon, H.]]	+	[[Category: Darbon, H]]
-	[[Category: Ferrat, G.]]	+	[[Category: Ferrat, G]]
-	[[Category: Kellenberger, C.]]	+	[[Category: Kellenberger, C]]
-	[[Category: Leone, P.]]	+	[[Category: Leone, P]]
-	[[Category: Roussel, A.]]	+	[[Category: Roussel, A]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]

OCA at 08:19, 8 October 2014

2014-10-08T08:19:29Z

←Older revision		Revision as of 08:19, 8 October 2014
Line 1:		Line 1:
-	[[~~Image~~:1wo9.~~png\|left\|200px~~]]	+	==Selective inhibition of trypsins by insect peptides: role of P6-P10 loop==
		+	<StructureSection load='1wo9' size='340' side='right' caption='[[1wo9]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WO9 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wo9 RCSB], [http://www.ebi.ac.uk/pdbsum/1wo9 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PMP-D2 and HI, two peptides from Locusta migratoria, were shown to belong to the family of tight-binding protease inhibitors. However, they interact weakly with bovine trypsin (K(i) around 100 nM) despite a trypsin-specific Arg at the primary specificity site P1. Here we demonstrate that they are potent inhibitors of midgut trypsins isolated from the same insect and of a fungal trypsin from Fusarium oxysporum (K(i) <or= 0.02 nM). Therefore, they display a selectivity not existing for the parent chymotrypsin inhibitor PMP-C. By NMR, we demonstrate that HI possesses a highly rigid structure similar to the crystal structure of a variant of PMP-D2 in complex with bovine alpha-chymotrypsin. The main difference with PMP-C is located in the region from residues 20 to 24 (positions P6-P10) that interacts with the loop containing Gly173 in chymotrypsin. The corresponding residue in mammalian trypsins is always a proline that may generate a steric clash with the inhibitor. The residues thought to confer selectivity were mutated with PMP-C as a model. The resulting analogue PMP-D2(K10W,P21A,W25A) loses some activity toward insect and fungal trypsins but is a more potent inhibitor of mammalian trypsins, corresponding to a decrease of selectivity. This work represents a first attempt in tuning the selectivity of natural peptidic serine protease inhibitors by mutating residues out of the reactive loop (P3-P'3).

-	~~{{STRUCTURE_1wo9\| PDB=1wo9 \| SCENE= }}~~	+	Selective inhibition of trypsins by insect peptides: role of P6-P10 loop.,Kellenberger C, Ferrat G, Leone P, Darbon H, Roussel A Biochemistry. 2003 Nov 25;42(46):13605-12. PMID:14622007<ref>PMID:14622007</ref>

-	~~===Selective inhibition~~ of ~~trypsins by insect peptides: role~~ of ~~P6-P10 loop===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14622007}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1wo9]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA].~~	+	</StructureSection>
	[[Category: Darbon, H.]]		[[Category: Darbon, H.]]
	[[Category: Ferrat, G.]]		[[Category: Ferrat, G.]]

OCA: Protected "1wo9" [edit=sysop:move=sysop]

2013-01-05T13:36:54Z

Protected "1wo9" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:36, 5 January 2013

OCA at 13:36, 5 January 2013

2013-01-05T13:36:50Z

←Older revision		Revision as of 13:36, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1wo9.png\|left\|200px]]		[[Image:1wo9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1wo9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1wo9\| PDB=1wo9 \| SCENE= }}		{{STRUCTURE_1wo9\| PDB=1wo9 \| SCENE= }}

	===Selective inhibition of trypsins by insect peptides: role of P6-P10 loop===		===Selective inhibition of trypsins by insect peptides: role of P6-P10 loop===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14622007}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14622007 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14622007}}		{{ABSTRACT_PUBMED_14622007}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA].	+	[[1wo9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA].
-		+
-	~~==Reference==~~	+
-	Selective inhibition of trypsins by insect peptides: role of P6-P10 loop., Kellenberger C, Ferrat G, Leone P, Darbon H, Roussel A, Biochemistry. 2003 Nov 25;42(46):13605-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14622007 14622007]	+
	[[Category: Darbon, H.]]		[[Category: Darbon, H.]]
	[[Category: Ferrat, G.]]		[[Category: Ferrat, G.]]
Line 30:		Line 15:
	[[Category: Roussel, A.]]		[[Category: Roussel, A.]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:19:32 2008''

OCA at 13:19, 28 July 2008

2008-07-28T13:19:39Z

←Older revision		Revision as of 13:19, 28 July 2008
Line 1:		Line 1:
-	[[Image:1wo9.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1wo9.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1wo9\| PDB=1wo9 \| SCENE= }}		{{STRUCTURE_1wo9\| PDB=1wo9 \| SCENE= }}

-	~~'''~~Selective inhibition of trypsins by insect peptides: role of P6-P10 loop~~'''~~	+	===Selective inhibition of trypsins by insect peptides: role of P6-P10 loop===


-	~~==Overview==~~	+	<!--
-	~~PMP-D2 and HI~~, ~~two peptides from Locusta migratoria~~, ~~were shown to belong~~ to the ~~family of tight-binding protease inhibitors. However, they interact weakly with bovine trypsin~~ (~~K(i) around 100 nM) despite a trypsin-specific Arg~~ at ~~the primary specificity site P1~~. ~~Here we demonstrate that they are potent inhibitors of midgut trypsins isolated from the same insect and of a fungal trypsin from Fusarium oxysporum (K(i) <or= 0~~.~~02 nM~~)~~. Therefore~~, they display a selectivity not existing for the parent chymotrypsin inhibitor PMP-C. By NMR, we demonstrate that HI possesses a highly rigid structure similar to the crystal structure of a variant of PMP-D2 in complex with bovine alpha-chymotrypsin. The main difference with PMP-C is ~~located in~~ the ~~region from residues 20 to 24 (positions P6-P10) that interacts with the loop containing Gly173 in chymotrypsin~~. ~~The corresponding residue in mammalian trypsins is always a proline that may generate a steric clash with the inhibitor. The residues thought to confer selectivity were mutated with PMP~~-~~C as a model. The resulting analogue PMP~~-D2(K10W,P21A,W25A) loses some activity toward insect and fungal trypsins but is a more potent inhibitor of mammalian trypsins, corresponding to a decrease of selectivity. This work represents a first attempt in tuning the selectivity of natural peptidic serine protease inhibitors by mutating residues out of the reactive loop (P3-P'3).	+	The line below this paragraph, {{ABSTRACT_PUBMED_14622007}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14622007 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14622007}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO9 OCA].

	==Reference==		==Reference==
Line 26:		Line 30:
	[[Category: Roussel, A.]]		[[Category: Roussel, A.]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 13~~:56:24 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:19:32 2008''