1y2t - Revision history

OCA at 13:37, 13 March 2024

2024-03-13T13:37:32Z

←Older revision		Revision as of 13:37, 13 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1y2u\|1y2u]]~~, [[~~1y2v~~\|~~1y2v~~]]~~, [[1y2w\|1y2w]], [[1y2x\|1y2x]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [https://pdbe.org/1y2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [https://www.ebi.ac.uk/pdbsum/1y2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [https://pdbe.org/1y2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [https://www.ebi.ac.uk/pdbsum/1y2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI]] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>	+	[https://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2t ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The lectin from the common mushroom Agaricus bisporus, the most popular edible species in Western countries, has potent antiproliferative effects on human epithelial cancer cells, without any apparent cytotoxicity. This property confers to it an important therapeutic potential as an antineoplastic agent. The three-dimensional structure of the lectin was determined by x-ray diffraction. The protein is a tetramer with 222 symmetry, and each monomer presents a novel fold with two beta sheets connected by a helix-loop-helix motif. Selectivity was studied by examining the binding of four monosaccharides and seven disaccharides in two different crystal forms. The T-antigen disaccharide, Galbeta1-3GalNAc, mediator of the antiproliferative effects of the protein, binds at a shallow depression on the surface of the molecule. The binding of N-acetylgalactosamine overlaps with that moiety of the T antigen, but surprisingly, N-acetylglucosamine, which differs from N-acetylgalactosamine only in the configuration of epimeric hydroxyl 4, binds at a totally different site on the opposite side of the helix-loop-helix motif. The lectin thus has two distinct binding sites per monomer that recognize the different configuration of a single epimeric hydroxyl. The structure of the protein and its two carbohydrate-binding sites are described in detail in this study.
-
-	The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl.,Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL J Biol Chem. 2005 Mar 18;280(11):10614-23. Epub 2004 Dec 13. PMID:15596442<ref>PMID:15596442</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1y2t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Capaldi, S]]	+	[[Category: Capaldi S]]
-	[[Category: Carrizo~~, M E~~]]	+	[[Category: Carrizo ME]]
-	[[Category: Irazoqui~~, F J~~]]	+	[[Category: Irazoqui FJ]]
-	[[Category: Monaco~~, H L~~]]	+	[[Category: Monaco HL]]
-	[[Category: Nores~~, G A~~]]	+	[[Category: Nores GA]]
-	[[Category: Perduca, M]]	+	[[Category: Perduca M]]
-	~~[[Category: Abl]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Mushroom]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 16:23, 3 November 2021

2021-11-03T16:23:05Z

←Older revision		Revision as of 16:23, 3 November 2021
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	<StructureSection load='1y2t' size='340' side='right'caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1y2t' size='340' side='right'caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [~~http~~://pdbe.org/1y2t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1y2t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [https://pdbe.org/1y2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [https://www.ebi.ac.uk/pdbsum/1y2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI]] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>	+	[[https://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI]] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:34, 29 January 2020

2020-01-29T16:34:54Z

←Older revision		Revision as of 16:34, 29 January 2020
Line 1:		Line 1:

	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin==		==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin==
-	<StructureSection load='1y2t' size='340' side='right' caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='1y2t' size='340' side='right'caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
		+	[[Category: Large Structures]]
	[[Category: Capaldi, S]]		[[Category: Capaldi, S]]
	[[Category: Carrizo, M E]]		[[Category: Carrizo, M E]]

OCA at 06:55, 18 April 2018

2018-04-18T06:55:24Z

←Older revision		Revision as of 06:55, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin==		==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin==
	<StructureSection load='1y2t' size='340' side='right' caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1y2t' size='340' side='right' caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://pdbe.org/1y2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://pdbe.org/1y2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2t_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2t_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:40, 11 September 2015

2015-09-11T12:40:02Z

←Older revision		Revision as of 12:40, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://pdbe.org/1y2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1y2t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:33, 25 December 2014

2014-12-25T16:33:52Z

←Older revision		Revision as of 16:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>		<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ABL_AGABI ABL_AGABI]] Lectin that recognizes O-linked galactose-beta-1,3-N-acetylgalactosamine, a disaccharide (Thomsen-Friedenreich antigen or T-disaccharide), present on cell surface glycoproteins. Can also bind galactose-beta-1,3-N-acetylglucosamine. Does not bind monosaccharides. Can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization it inhibits nuclear import of nuclear localization signal dependent proteins. Inhibits proliferation of malignant cells without cytotoxicity for normal cells.<ref>PMID:4343164</ref> <ref>PMID:8402638</ref> <ref>PMID:9988731</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
-	[[Category: Capaldi, S.]]	+	[[Category: Capaldi, S]]
-	[[Category: Carrizo, M E.]]	+	[[Category: Carrizo, M E]]
-	[[Category: Irazoqui, F J.]]	+	[[Category: Irazoqui, F J]]
-	[[Category: Monaco, H L.]]	+	[[Category: Monaco, H L]]
-	[[Category: Nores, G A.]]	+	[[Category: Nores, G A]]
-	[[Category: Perduca, M.]]	+	[[Category: Perduca, M]]
	[[Category: Abl]]		[[Category: Abl]]
-	[[Category: Agaricus bisporus]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Mushroom]]		[[Category: Mushroom]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 19:48, 29 September 2014

2014-09-29T19:48:58Z

←Older revision		Revision as of 19:48, 29 September 2014
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-	[[~~Image:~~1y2t.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the common edible mushroom (Agaricus bisporus) lectin==
		+	<StructureSection load='1y2t' size='340' side='right' caption='[[1y2t]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y2T FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y2u\|1y2u]], [[1y2v\|1y2v]], [[1y2w\|1y2w]], [[1y2x\|1y2x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y2t RCSB], [http://www.ebi.ac.uk/pdbsum/1y2t PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2t_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The lectin from the common mushroom Agaricus bisporus, the most popular edible species in Western countries, has potent antiproliferative effects on human epithelial cancer cells, without any apparent cytotoxicity. This property confers to it an important therapeutic potential as an antineoplastic agent. The three-dimensional structure of the lectin was determined by x-ray diffraction. The protein is a tetramer with 222 symmetry, and each monomer presents a novel fold with two beta sheets connected by a helix-loop-helix motif. Selectivity was studied by examining the binding of four monosaccharides and seven disaccharides in two different crystal forms. The T-antigen disaccharide, Galbeta1-3GalNAc, mediator of the antiproliferative effects of the protein, binds at a shallow depression on the surface of the molecule. The binding of N-acetylgalactosamine overlaps with that moiety of the T antigen, but surprisingly, N-acetylglucosamine, which differs from N-acetylgalactosamine only in the configuration of epimeric hydroxyl 4, binds at a totally different site on the opposite side of the helix-loop-helix motif. The lectin thus has two distinct binding sites per monomer that recognize the different configuration of a single epimeric hydroxyl. The structure of the protein and its two carbohydrate-binding sites are described in detail in this study.

-	~~{{STRUCTURE_1y2t\| PDB=1y2t \| SCENE= }}~~	+	The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl.,Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL J Biol Chem. 2005 Mar 18;280(11):10614-23. Epub 2004 Dec 13. PMID:15596442<ref>PMID:15596442</ref>

-	~~===Crystal structure~~ of the ~~common edible mushroom (Agaricus bisporus) lectin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15596442}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1y2t]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Agaricus_bisporus Agaricus bisporus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015596442<~~/~~ref~~><~~ref group="xtra">PMID:015039564</ref><references group="xtra"~~/>	+
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Capaldi, S.]]		[[Category: Capaldi, S.]]

OCA at 12:12, 23 January 2013

2013-01-23T12:12:19Z

←Older revision		Revision as of 12:12, 23 January 2013
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	[[Image:1y2t.png\|left\|200px]]		[[Image:1y2t.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1y2t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1y2t\| PDB=1y2t \| SCENE= }}		{{STRUCTURE_1y2t\| PDB=1y2t \| SCENE= }}

	===Crystal structure of the common edible mushroom (Agaricus bisporus) lectin===		===Crystal structure of the common edible mushroom (Agaricus bisporus) lectin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15596442}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15596442 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15596442}}		{{ABSTRACT_PUBMED_15596442}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15596442~~</ref><ref group="xtra">PMID:~~15039564~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015596442</ref><ref group="xtra">PMID:015039564</ref><references group="xtra"/>
	[[Category: Agaricus bisporus]]		[[Category: Agaricus bisporus]]
	[[Category: Capaldi, S.]]		[[Category: Capaldi, S.]]

OCA: Protected "1y2t" [edit=sysop:move=sysop]

2011-03-14T23:07:19Z

Protected "1y2t" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:07, 14 March 2011

OCA at 23:07, 14 March 2011

2011-03-14T23:07:18Z

←Older revision		Revision as of 23:07, 14 March 2011
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-	{{Seed}}
	[[Image:1y2t.png\|left\|200px]]		[[Image:1y2t.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~1Y2T~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA].	+	[[1y2t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2T OCA].

	==Reference==		==Reference==
Line 35:		Line 34:
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Mushroom]]		[[Category: Mushroom]]
-		+	[[Category: Sugar binding protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 21:53:44 2009''~~	+