1z3c - Revision history

OCA at 07:03, 23 August 2023

2023-08-23T07:03:28Z

←Older revision		Revision as of 07:03, 23 August 2023
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	<StructureSection load='1z3c' size='340' side='right'caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1z3c' size='340' side='right'caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enccn Enccn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SA8:S~~-~~5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=6035 ENCCN])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [https://pdbe.org/1z3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [https://www.ebi.ac.uk/pdbsum/1z3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3c ProSAT]</span></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/mRNA_(guanine~~-~~N(7)~~-)-~~methyltransferase mRNA (guanine~~-~~N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [~~http~~://pdbe.org/1z3c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1z3c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3c ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU]] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>	+	[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 1z3c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1z3c" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[MRNA capping enzyme\|MRNA capping enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enccn~~]]	+	[[Category: Encephalitozoon cuniculi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fabrega, C]]	+	[[Category: Fabrega C]]
-	[[Category: Hausmann, S]]	+	[[Category: Hausmann S]]
-	[[Category: Lima~~, C D~~]]	+	[[Category: Lima CD]]
-	[[Category: Schneller~~, S W~~]]	+	[[Category: Schneller SW]]
-	[[Category: Shuman, S]]	+	[[Category: Shuman S]]
-	[[Category: Zhang, S]]	+	[[Category: Zhang S]]
-	~~[[Category: Azoadomet]]~~	+
-	~~[[Category: Cap]]~~	+
-	~~[[Category: M7g]]~~	+
-	~~[[Category: Messenger rna cap]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:57, 20 January 2021

2021-01-20T08:57:30Z

←Older revision		Revision as of 08:57, 20 January 2021
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	==Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet==		==Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet==
-	<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1z3c' size='340' side='right'caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://pdbe.org/1z3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://pdbe.org/1z3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1z3c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1z3c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[MRNA capping enzyme\|MRNA capping enzyme]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Enccn]]		[[Category: Enccn]]
		+	[[Category: Large Structures]]
	[[Category: Fabrega, C]]		[[Category: Fabrega, C]]
	[[Category: Hausmann, S]]		[[Category: Hausmann, S]]

OCA at 06:49, 25 April 2018

2018-04-25T06:49:37Z

←Older revision		Revision as of 06:49, 25 April 2018
Line 1:		Line 1:
		+
	==Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet==		==Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet==
	<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://pdbe.org/1z3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://pdbe.org/1z3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z3c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:00, 12 September 2015

2015-09-12T00:00:38Z

←Older revision		Revision as of 00:00, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Encephalitozoon_cuniculi Encephalitozoon cuniculi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ~~Encephalitozoon cuniculi~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://pdbe.org/1z3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1z3c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Encephalitozoon cuniculi~~]]	+	[[Category: Enccn]]
	[[Category: Fabrega, C]]		[[Category: Fabrega, C]]
	[[Category: Hausmann, S]]		[[Category: Hausmann, S]]

OCA at 21:08, 25 December 2014

2014-12-25T21:08:45Z

←Older revision		Revision as of 21:08, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU]] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
-	[[Category: Fabrega, C.]]	+	[[Category: Fabrega, C]]
-	[[Category: Hausmann, S.]]	+	[[Category: Hausmann, S]]
-	[[Category: Lima, C D.]]	+	[[Category: Lima, C D]]
-	[[Category: Schneller, S W.]]	+	[[Category: Schneller, S W]]
-	[[Category: Shuman, S.]]	+	[[Category: Shuman, S]]
-	[[Category: Zhang, S.]]	+	[[Category: Zhang, S]]
	[[Category: Azoadomet]]		[[Category: Azoadomet]]
	[[Category: Cap]]		[[Category: Cap]]

OCA at 20:34, 29 September 2014

2014-09-29T20:34:06Z

←Older revision		Revision as of 20:34, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1z3c.~~png~~\|~~left~~\|200px]]	+	==Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet==
		+	<StructureSection load='1z3c' size='340' side='right' caption='[[1z3c]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z3C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SA8:S-5-AZAMETHIONINE-5-DEOXYADENOSINE'>SA8</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri2\|1ri2]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z3c RCSB], [http://www.ebi.ac.uk/pdbsum/1z3c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase Ecm1 has been characterized structurally but not biochemically. Here we show that purified Ecm1 is a monomeric protein that catalyzes methyl transfer from S-adenosylmethionine (AdoMet) to GTP. The reaction is cofactor-independent and optimal at pH 7.5. Ecm1 also methylates GpppA, GDP, and dGTP but not ATP, CTP, UTP, ITP, or m(7)GTP. The affinity of Ecm1 for the cap dinucleotide GpppA (K 0.1 mm) is higher than that for GTP (K(m) 1 mm) or GDP (K(m) 2.4 mm). Methylation of GTP by Ecm1 in the presence of 5 microm AdoMet is inhibited by the reaction product AdoHcy (IC(50) 4 microm) and by substrate analogs sinefungin (IC(50) 1.5 microm), aza-AdoMet (IC(50) 100 microm), and carbocyclic aza-AdoMet (IC(50) 35 microm). The crystal structure of an Ecm1.aza-AdoMet binary complex reveals that the inhibitor occupies the same site as AdoMet. Structure-function analysis of Ecm1 by alanine scanning and conservative substitutions identified functional groups necessary for methyltransferase activity in vivo. Amino acids Lys-54, Asp-70, Asp-78, and Asp-94, which comprise the AdoMet-binding site, and Phe-141, which contacts the cap guanosine, are essential for cap methyltransferase activity in vitro.

-	~~{{STRUCTURE_1z3c\| PDB=1z3c \| SCENE= }}~~	+	Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase: methyl acceptor specificity, inhibition BY S-adenosylmethionine analogs, and structure-guided mutational analysis.,Hausmann S, Zheng S, Fabrega C, Schneller SW, Lima CD, Shuman S J Biol Chem. 2005 May 27;280(21):20404-12. Epub 2005 Mar 9. PMID:15760890<ref>PMID:15760890</ref>

-	~~===Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15760890}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1z3c]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015760890<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]

OCA: Protected "1z3c" [edit=sysop:move=sysop]

2013-01-05T17:29:32Z

Protected "1z3c" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:29, 5 January 2013

OCA at 17:29, 5 January 2013

2013-01-05T17:29:26Z

←Older revision		Revision as of 17:29, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1z3c.png\|left\|200px]]		[[Image:1z3c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1z3c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1z3c\| PDB=1z3c \| SCENE= }}		{{STRUCTURE_1z3c\| PDB=1z3c \| SCENE= }}

	===Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet===		===Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15760890}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15760890 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15760890}}		{{ABSTRACT_PUBMED_15760890}}

	==About this Structure==		==About this Structure==
-	~~1Z3C~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA].	+	[[1z3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15760890~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015760890</ref><references group="xtra"/>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]
Line 37:		Line 25:
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rna]]		[[Category: Rna]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 07:39:58 2009''~~	+

OCA at 05:39, 18 February 2009

2009-02-18T05:39:59Z

←Older revision		Revision as of 05:39, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1Z3C is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA].	+	1Z3C is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3C OCA].

	==Reference==		==Reference==
-	Encephalitozoon cuniculi mRNA cap (guanine N-7) methyltransferase: methyl acceptor specificity, inhibition BY S-adenosylmethionine analogs, and structure-guided mutational analysis., Hausmann S, Zheng S, Fabrega C, Schneller SW, Lima CD, Shuman S, J Biol Chem. 2005 May 27;280(21):20404-12. Epub 2005 Mar 9. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15760890 15760890]~~	+	<ref group="xtra">PMID:15760890</ref><references group="xtra"/>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
-	[[Category: Single protein]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]
	[[Category: Hausmann, S.]]		[[Category: Hausmann, S.]]
Line 39:		Line 38:
	[[Category: Rna]]		[[Category: Rna]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 14~~:05:~~41 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:39:58 2009''

OCA at 11:05, 29 July 2008

2008-07-29T11:05:49Z

←Older revision		Revision as of 11:05, 29 July 2008
Line 1:		Line 1:
-	[[Image:1z3c.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1z3c.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1z3c\| PDB=1z3c \| SCENE= }}		{{STRUCTURE_1z3c\| PDB=1z3c \| SCENE= }}

-	~~'''~~Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet~~'''~~	+	===Encephalitozooan cuniculi mRNA Cap (Guanine-N7) Methyltransferasein complexed with AzoAdoMet===


-	~~==Overview==~~	+	<!--
-	~~The Encephalitozoon cuniculi mRNA cap (guanine N~~-~~7) methyltransferase Ecm1 has been characterized structurally but not biochemically. Here we show that purified Ecm1 is a monomeric protein that catalyzes methyl transfer from S~~-~~adenosylmethionine (AdoMet) to GTP.~~ The ~~reaction is cofactor-independent and optimal at pH 7.5. Ecm1 also methylates GpppA~~, ~~GDP~~, ~~and dGTP but not ATP, CTP, UTP, ITP, or m(7)GTP. The affinity of Ecm1 for~~ the ~~cap dinucleotide GpppA~~ (~~K 0~~.~~1 mm) is higher than that for GTP (K(m) 1 mm) or GDP (K(m) 2~~.~~4 mm~~)~~. Methylation of GTP by Ecm1 in the presence of 5 microm AdoMet~~ is ~~inhibited by~~ the ~~reaction product AdoHcy (IC(50) 4 microm) and by substrate analogs sinefungin (IC(50) 1~~.~~5 microm), aza~~-~~AdoMet (IC(50) 100 microm), and carbocyclic aza~~-AdoMet (IC(50) 35 microm). The crystal structure of an Ecm1.aza-AdoMet binary complex reveals that the inhibitor occupies the same site as AdoMet. Structure-function analysis of Ecm1 by alanine scanning and conservative substitutions identified functional groups necessary for methyltransferase activity in vivo. Amino acids Lys-54, Asp-70, Asp-78, and Asp-94, which comprise the AdoMet-binding site, and Phe-141, which contacts the cap guanosine, are essential for cap methyltransferase activity in vitro.	+	The line below this paragraph, {{ABSTRACT_PUBMED_15760890}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15760890 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15760890}}

	==About this Structure==		==About this Structure==
Line 34:		Line 38:
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rna]]		[[Category: Rna]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 17~~:07:57 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:05:41 2008''