2a8l - Revision history

OCA at 09:11, 14 February 2024

2024-02-14T09:11:47Z

←Older revision		Revision as of 09:11, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2a8l' size='340' side='right'caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2a8l' size='340' side='right'caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8L FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">C20orf13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [https://pdbe.org/2a8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [https://www.ebi.ac.uk/pdbsum/2a8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [https://pdbe.org/2a8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [https://www.ebi.ac.uk/pdbsum/2a8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.	+	[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8l ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8l ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.
-
-	Crystal structure of human Taspase1, a crucial protease regulating the function of MLL.,Khan JA, Dunn BM, Tong L Structure. 2005 Oct;13(10):1443-52. PMID:16216576<ref>PMID:16216576</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2a8l" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dunn~~, B M~~]]	+	[[Category: Dunn BM]]
-	[[Category: Khan~~, J A~~]]	+	[[Category: Khan JA]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	~~[[Category: Asparaginase]]~~	+
-	~~[[Category: Glycosylasparaginase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Leukemia]]~~	+
-	~~[[Category: Mll]]~~	+
-	~~[[Category: Taspase1~~]]	+

OCA at 08:27, 27 January 2021

2021-01-27T08:27:39Z

←Older revision		Revision as of 08:27, 27 January 2021
Line 1:		Line 1:

	==Crystal structure of Human Taspase1 (T234A mutant)==		==Crystal structure of Human Taspase1 (T234A mutant)==
-	<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2a8l' size='340' side='right'caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2A8L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8L FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [~~http~~://pdbe.org/2a8l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2a8l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [https://pdbe.org/2a8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [https://www.ebi.ac.uk/pdbsum/2a8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.	+	[[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8l_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8l_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Dunn, B M]]		[[Category: Dunn, B M]]
	[[Category: Khan, J A]]		[[Category: Khan, J A]]

OCA at 13:57, 12 October 2017

2017-10-12T13:57:26Z

←Older revision		Revision as of 13:57, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of Human Taspase1 (T234A mutant)==		==Crystal structure of Human Taspase1 (T234A mutant)==
	<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://pdbe.org/2a8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://pdbe.org/2a8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:40, 10 February 2016

2016-02-10T06:40:23Z

←Older revision		Revision as of 06:40, 10 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8l ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:21, 11 September 2015

2015-09-11T06:21:32Z

←Older revision		Revision as of 06:21, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8L FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://pdbe.org/2a8l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2a8l" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Dunn, B M]]		[[Category: Dunn, B M]]
	[[Category: Khan, J A]]		[[Category: Khan, J A]]

OCA at 12:30, 25 December 2014

2014-12-25T12:30:08Z

←Older revision		Revision as of 12:30, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8L FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8L FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dunn, B M.]]	+	[[Category: Dunn, B M]]
-	[[Category: Khan, J A.]]	+	[[Category: Khan, J A]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Glycosylasparaginase]]		[[Category: Glycosylasparaginase]]

OCA at 02:01, 30 September 2014

2014-09-30T02:01:05Z

←Older revision		Revision as of 02:01, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2a8l.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Human Taspase1 (T234A mutant)==
		+	<StructureSection load='2a8l' size='340' side='right' caption='[[2a8l]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8L FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8m\|2a8m]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8l RCSB], [http://www.ebi.ac.uk/pdbsum/2a8l PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8l_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.

-	~~{{STRUCTURE_2a8l\| PDB=2a8l \| SCENE= }}~~	+	Crystal structure of human Taspase1, a crucial protease regulating the function of MLL.,Khan JA, Dunn BM, Tong L Structure. 2005 Oct;13(10):1443-52. PMID:16216576<ref>PMID:16216576</ref>

-	~~===Crystal structure~~ of ~~Human Taspase1 (T234A mutant)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16216576}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2a8l]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016216576<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]

OCA: Protected "2a8l" [edit=sysop:move=sysop]

2013-01-06T11:14:21Z

Protected "2a8l" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 6 January 2013

OCA at 11:14, 6 January 2013

2013-01-06T11:14:18Z

←Older revision		Revision as of 11:14, 6 January 2013
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-	{{Seed}}
	[[Image:2a8l.png\|left\|200px]]		[[Image:2a8l.png\|left\|200px]]

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	{{STRUCTURE_2a8l\| PDB=2a8l \| SCENE= }}		{{STRUCTURE_2a8l\| PDB=2a8l \| SCENE= }}

	===Crystal structure of Human Taspase1 (T234A mutant)===		===Crystal structure of Human Taspase1 (T234A mutant)===

-
-	<!--
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	{{ABSTRACT_PUBMED_16216576}}		{{ABSTRACT_PUBMED_16216576}}

	==About this Structure==		==About this Structure==
-	~~2A8L~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA].	+	[[2a8l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16216576~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016216576</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]
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	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Glycosylasparaginase]]		[[Category: Glycosylasparaginase]]
		+	[[Category: Hydrolase]]
	[[Category: Leukemia]]		[[Category: Leukemia]]
	[[Category: Mll]]		[[Category: Mll]]
	[[Category: Taspase1]]		[[Category: Taspase1]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:13:08 2009''

OCA at 14:13, 17 February 2009

2009-02-17T14:13:10Z

←Older revision		Revision as of 14:13, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2A8L is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA].	+	2A8L is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8L OCA].

	==Reference==		==Reference==
-	~~Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16216576 16216576]~~	+	<ref group="xtra">PMID:16216576</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]
	[[Category: Khan, J A.]]		[[Category: Khan, J A.]]
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	[[Category: Taspase1]]		[[Category: Taspase1]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 22~~:27:~~52 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:13:08 2009''