2a8m - Revision history

OCA at 09:11, 14 February 2024

2024-02-14T09:11:50Z

←Older revision		Revision as of 09:11, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2a8m' size='340' side='right'caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2a8m' size='340' side='right'caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">C20orf13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [https://pdbe.org/2a8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [https://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [https://pdbe.org/2a8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [https://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.	+	[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8m ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.
-
-	Crystal structure of human Taspase1, a crucial protease regulating the function of MLL.,Khan JA, Dunn BM, Tong L Structure. 2005 Oct;13(10):1443-52. PMID:16216576<ref>PMID:16216576</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2a8m" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dunn~~, B M~~]]	+	[[Category: Dunn BM]]
-	[[Category: Khan~~, J A~~]]	+	[[Category: Khan JA]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	~~[[Category: Asparaginase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Leukemia]]~~	+
-	~~[[Category: Mll]]~~	+
-	~~[[Category: Taspase~~]]	+

OCA at 08:27, 27 January 2021

2021-01-27T08:27:40Z

←Older revision		Revision as of 08:27, 27 January 2021
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	==Crystal Structure of Human Taspase1 (T234S mutant)==		==Crystal Structure of Human Taspase1 (T234S mutant)==
-	<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='2a8m' size='340' side='right'caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2A8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [~~http~~://pdbe.org/2a8m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [https://pdbe.org/2a8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [https://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.	+	[[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8m_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Dunn, B M]]		[[Category: Dunn, B M]]
	[[Category: Khan, J A]]		[[Category: Khan, J A]]

OCA at 16:14, 12 October 2017

2017-10-12T16:14:10Z

←Older revision		Revision as of 16:14, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of Human Taspase1 (T234S mutant)==		==Crystal Structure of Human Taspase1 (T234S mutant)==
	<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://pdbe.org/2a8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://pdbe.org/2a8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 15:20, 8 February 2016

2016-02-08T15:20:38Z

←Older revision		Revision as of 15:20, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:53, 10 September 2015

2015-09-10T12:53:57Z

←Older revision		Revision as of 12:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://pdbe.org/2a8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2a8m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Dunn, B M]]		[[Category: Dunn, B M]]
	[[Category: Khan, J A]]		[[Category: Khan, J A]]

OCA at 00:17, 25 December 2014

2014-12-25T00:17:51Z

←Older revision		Revision as of 00:17, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dunn, B M.]]	+	[[Category: Dunn, B M]]
-	[[Category: Khan, J A.]]	+	[[Category: Khan, J A]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 02:54, 30 September 2014

2014-09-30T02:54:36Z

←Older revision		Revision as of 02:54, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2a8m.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Human Taspase1 (T234S mutant)==
		+	<StructureSection load='2a8m' size='340' side='right' caption='[[2a8m]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A8M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8i\|2a8i]], [[2a8j\|2a8j]], [[2a8l\|2a8l]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a8m RCSB], [http://www.ebi.ac.uk/pdbsum/2a8m PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8m_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.

-	~~{{STRUCTURE_2a8m\| PDB=2a8m \| SCENE= }}~~	+	Crystal structure of human Taspase1, a crucial protease regulating the function of MLL.,Khan JA, Dunn BM, Tong L Structure. 2005 Oct;13(10):1443-52. PMID:16216576<ref>PMID:16216576</ref>

-	~~===Crystal Structure~~ of ~~Human Taspase1 (T234S mutant)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16216576}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2a8m]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016216576<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]

OCA: Protected "2a8m" [edit=sysop:move=sysop]

2013-01-06T12:05:12Z

Protected "2a8m" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:05, 6 January 2013

OCA at 12:05, 6 January 2013

2013-01-06T12:05:10Z

←Older revision		Revision as of 12:05, 6 January 2013
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-	{{Seed}}
	[[Image:2a8m.png\|left\|200px]]		[[Image:2a8m.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2a8m", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2a8m\| PDB=2a8m \| SCENE= }}		{{STRUCTURE_2a8m\| PDB=2a8m \| SCENE= }}

	===Crystal Structure of Human Taspase1 (T234S mutant)===		===Crystal Structure of Human Taspase1 (T234S mutant)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16216576}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16216576 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16216576}}		{{ABSTRACT_PUBMED_16216576}}

	==About this Structure==		==About this Structure==
-	~~2A8M~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].	+	[[2a8m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16216576~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016216576</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]
Line 29:		Line 17:
	[[Category: Tong, L.]]		[[Category: Tong, L.]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
		+	[[Category: Hydrolase]]
	[[Category: Leukemia]]		[[Category: Leukemia]]
	[[Category: Mll]]		[[Category: Mll]]
	[[Category: Taspase]]		[[Category: Taspase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:58:27 2009''

OCA at 21:58, 16 February 2009

2009-02-16T21:58:28Z

←Older revision		Revision as of 21:58, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2A8M is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].	+	2A8M is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].

	==Reference==		==Reference==
-	~~Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16216576 16216576]~~	+	<ref group="xtra">PMID:16216576</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Dunn, B M.]]		[[Category: Dunn, B M.]]
	[[Category: Khan, J A.]]		[[Category: Khan, J A.]]
Line 34:		Line 33:
	[[Category: Taspase]]		[[Category: Taspase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 04~~:19:~~19 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:58:27 2009''