2ab3 - Revision history

OCA at 16:31, 13 December 2023

2023-12-13T16:31:58Z

←Older revision		Revision as of 16:31, 13 December 2023
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	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
-	<StructureSection load='2ab3' size='340' side='right'caption='[[2ab3~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='2ab3' size='340' side='right'caption='[[2ab3]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [https://pdbe.org/2ab3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [https://pdbe.org/2ab3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab3 ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Darby~~, M K~~]]	+	[[Category: Darby MK]]
-	[[Category: Germann~~, M W~~]]	+	[[Category: Germann MW]]
-	[[Category: Mishra~~, S H~~]]	+	[[Category: Mishra SH]]
-	[[Category: Shelley~~, C M]]~~	+	[[Category: Shelley CM]]
-	~~[[Category: Beta beta alpha]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rreiib-tr]]~~	+
-	~~[[Category: Zinc finger protein~~]]	+

OCA at 14:38, 17 November 2021

2021-11-17T14:38:39Z

←Older revision		Revision as of 14:38, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2ab3' size='340' side='right'caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab3' size='340' side='right'caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [~~http~~://pdbe.org/2ab3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ab3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [https://pdbe.org/2ab3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ab3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:02, 13 February 2020

2020-02-13T05:02:13Z

←Older revision		Revision as of 05:02, 13 February 2020
Line 1:		Line 1:

	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
-	<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='2ab3' size='340' side='right'caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Darby, M K]]		[[Category: Darby, M K]]
	[[Category: Germann, M W]]		[[Category: Germann, M W]]

OCA at 06:51, 9 May 2018

2018-05-09T06:51:19Z

←Older revision		Revision as of 06:51, 9 May 2018
Line 1:		Line 1:
		+
	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==		==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
	<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [http://pdbe.org/2ab3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [http://pdbe.org/2ab3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ab3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:44, 10 September 2015

2015-09-10T20:44:37Z

←Older revision		Revision as of 20:44, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [http://pdbe.org/2ab3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab3 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ab3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Darby, M K]]		[[Category: Darby, M K]]
	[[Category: Germann, M W]]		[[Category: Germann, M W]]

OCA at 16:15, 15 January 2015

2015-01-15T16:15:04Z

←Older revision		Revision as of 16:15, 15 January 2015
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Darby, M K.]]	+	[[Category: Darby, M K]]
-	[[Category: Germann, M W.]]	+	[[Category: Germann, M W]]
-	[[Category: Mishra, S H.]]	+	[[Category: Mishra, S H]]
-	[[Category: Shelley, C M.]]	+	[[Category: Shelley, C M]]
	[[Category: Beta beta alpha]]		[[Category: Beta beta alpha]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]
	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]

OCA at 08:21, 8 October 2014

2014-10-08T08:21:08Z

←Older revision		Revision as of 08:21, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2ab3.~~png~~\|~~left\|200px~~]]	+	==Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins==
		+	<StructureSection load='2ab3' size='340' side='right' caption='[[2ab3]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AB3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ab3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ab3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ab3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ab3 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Rev responsive element (RRE), a part of unspliced human immunodeficiency virus (HIV) RNA, serves a crucial role in the production of infectious HIV virions. The viral protein Rev binds to RRE and facilitates transport of mRNA to the cytoplasm. Inhibition of the Rev-RRE interaction disrupts the viral life cycle. Using a phage display protocol, dual zinc finger proteins (ZNFs) were generated that bind specifically to RREIIB at the high affinity Rev binding site. These proteins were further shortened and simplified, and they still retained their RNA binding affinity. The solution structures of ZNF29 and a mutant, ZNF29G29R, have been determined by nuclear magnetic resonance (NMR) spectroscopy. Both proteins form C(2)H(2)-type zinc fingers with essentially identical structures. RNA protein interactions were evaluated quantitatively by isothermal titration calorimetry, which revealed dissociation constants (K(d)'s) in the nanomolar range. The interaction with the RNA is dependent upon the zinc finger structure; in the presence of EDTA, RNA binding is abolished. For both proteins, RNA binding is mediated by the alpha-helical portion of the zinc fingers and target the bulge region of RREIIB-TR. However, ZNF29G29R exhibits significantly stronger binding to the RNA target than ZNF29; this illustrates that the binding of the zinc finger scaffold is amenable to further improvements.

-	~~{{STRUCTURE_2ab3\| PDB=2ab3 \| SCENE= }}~~	+	Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins.,Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW Biopolymers. 2006 Nov;83(4):352-64. PMID:16826557<ref>PMID:16826557</ref>

-	~~===Solution structures and characterization~~ of ~~HIV RRE IIB RNA targeting zinc finger proteins===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16826557}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ab3]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016826557<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Darby, M K.]]		[[Category: Darby, M K.]]

OCA: Protected "2ab3" [edit=sysop:move=sysop]

2013-01-06T10:53:07Z

Protected "2ab3" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 6 January 2013

OCA at 10:53, 6 January 2013

2013-01-06T10:53:05Z

←Older revision		Revision as of 10:53, 6 January 2013
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-	{{Seed}}
	[[Image:2ab3.png\|left\|200px]]		[[Image:2ab3.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2ab3\| PDB=2ab3 \| SCENE= }}		{{STRUCTURE_2ab3\| PDB=2ab3 \| SCENE= }}

	===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===		===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===

-
-	<!--
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	{{ABSTRACT_PUBMED_16826557}}		{{ABSTRACT_PUBMED_16826557}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA].	+	[[2ab3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA].

	==Reference==		==Reference==
-	Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins., Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW, Biopolymers. 2006 Nov;83(4):352-64. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/16826557 16826557~~]	+	<ref group="xtra">PMID:016826557</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
	[[Category: Darby, M K.]]		[[Category: Darby, M K.]]
	[[Category: Germann, M W.]]		[[Category: Germann, M W.]]
Line 29:		Line 18:
	[[Category: Shelley, C M.]]		[[Category: Shelley, C M.]]
	[[Category: Beta beta alpha]]		[[Category: Beta beta alpha]]
		+	[[Category: Rna binding protein]]
	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:03:34 2008''

OCA at 09:03, 28 July 2008

2008-07-28T09:03:36Z

←Older revision		Revision as of 09:03, 28 July 2008
Line 1:		Line 1:
-	[[Image:2ab3.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ab3.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2ab3\| PDB=2ab3 \| SCENE= }}		{{STRUCTURE_2ab3\| PDB=2ab3 \| SCENE= }}

-	~~'''~~Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins~~'''~~	+	===Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins===


-	~~==Overview==~~	+	<!--
-	The ~~Rev responsive element (RRE)~~, ~~a part of unspliced human immunodeficiency virus (HIV) RNA~~, ~~serves a crucial role in~~ the ~~production of infectious HIV virions. The viral protein Rev binds to RRE and facilitates transport of mRNA~~ to the ~~cytoplasm. Inhibition of the Rev-RRE interaction disrupts the viral life cycle. Using a phage display protocol, dual zinc finger proteins~~ (~~ZNFs) were generated that bind specifically to RREIIB~~ at ~~the high affinity Rev binding site~~. ~~These proteins were further shortened and simplified, and they still retained their RNA binding affinity~~. ~~The solution structures of ZNF29 and a mutant, ZNF29G29R, have been determined by nuclear magnetic resonance (NMR~~) ~~spectroscopy. Both proteins form C(2)H(2)-type zinc fingers with essentially identical structures. RNA protein interactions were evaluated quantitatively by isothermal titration calorimetry~~, ~~which revealed dissociation constants (K(d)'s) in the nanomolar range. The interaction with the RNA~~ is ~~dependent upon~~ the ~~zinc finger structure; in the presence of EDTA, RNA binding is abolished~~. ~~For both proteins, RNA binding is mediated by the alpha~~-~~helical portion of the zinc fingers and target the bulge region of RREIIB~~-~~TR. However, ZNF29G29R exhibits significantly stronger binding to the RNA target than ZNF29; this illustrates that the binding of the zinc finger scaffold is amenable to further improvements.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_16826557}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16826557 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16826557}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA].

	==Reference==		==Reference==
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	[[Category: Rreiib-tr]]		[[Category: Rreiib-tr]]
	[[Category: Zinc finger protein]]		[[Category: Zinc finger protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 18~~:50:14 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:03:34 2008''