2abh - Revision history

OCA at 09:12, 14 February 2024

2024-02-14T09:12:19Z

←Older revision		Revision as of 09:12, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ABH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [https://pdbe.org/2abh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [https://www.ebi.ac.uk/pdbsum/2abh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [https://pdbe.org/2abh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [https://www.ebi.ac.uk/pdbsum/2abh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI]] Part of the ABC transporter complex PstSACB involved in phosphate import.	+	[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI] Part of the ABC transporter complex PstSACB involved in phosphate import.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2abh ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2abh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Electrostatic interactions are among the key forces determining the structure and function of proteins. These are exemplified in the liganded form of the receptor, a phosphate binding protein from Escherichia coli. The phosphate, completely dehydrated and buried in the receptor, is bound by 12 hydrogen bonds as well as a salt link with Arg 135. We have modulated the ionic attraction while preserving the hydrogen bonds by mutating Asp 137, also salt linked to Arg 135, to Asn, Gly or Thr. High-resolution crystallographic analysis revealed that Gly and Thr (but not Asn) mutant proteins have incorporated a more electronegative Cl- in place of the Asp carboxylate. That no dramatic effect on phosphate affinity was produced by these ionic perturbations indicates a major role for hydrogen bonds and other local dipoles in the binding and charge stabilization of ionic ligands.
-
-	Modulation of a salt link does not affect binding of phosphate to its specific active transport receptor.,Yao N, Ledvina PS, Choudhary A, Quiocho FA Biochemistry. 1996 Feb 20;35(7):2079-85. PMID:8652549<ref>PMID:8652549</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2abh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Phosphate-binding protein\|Phosphate-binding protein]]		*[[Phosphate-binding protein\|Phosphate-binding protein]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Choudhary, A]]	+	[[Category: Choudhary A]]
-	[[Category: Ledvina~~, P S~~]]	+	[[Category: Ledvina PS]]
-	[[Category: Quiocho~~, F A~~]]	+	[[Category: Quiocho FA]]
-	[[Category: Yao, N]]	+	[[Category: Yao N]]
-	~~[[Category: Phosphotransferase]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 14:38, 17 November 2021

2021-11-17T14:38:48Z

←Older revision		Revision as of 14:38, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2abh' size='340' side='right'caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2abh' size='340' side='right'caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ABH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [~~http~~://pdbe.org/2abh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2abh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [https://pdbe.org/2abh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [https://www.ebi.ac.uk/pdbsum/2abh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI]] Part of the ABC transporter complex PstSACB involved in phosphate import.	+	[[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI]] Part of the ABC transporter complex PstSACB involved in phosphate import.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:19, 13 February 2020

2020-02-13T05:19:18Z

←Older revision		Revision as of 05:19, 13 February 2020
Line 1:		Line 1:

	==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==		==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==
-	<StructureSection load='2abh' size='340' side='right' caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='2abh' size='340' side='right'caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Choudhary, A]]		[[Category: Choudhary, A]]
	[[Category: Ledvina, P S]]		[[Category: Ledvina, P S]]

OCA at 07:10, 9 May 2018

2018-05-09T07:10:14Z

←Older revision		Revision as of 07:10, 9 May 2018
Line 1:		Line 1:
		+
	==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==		==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==
	<StructureSection load='2abh' size='340' side='right' caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2abh' size='340' side='right' caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://pdbe.org/2abh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://pdbe.org/2abh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2abh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/2abh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/2abh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2abh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:30, 11 September 2015

2015-09-11T11:30:45Z

←Older revision		Revision as of 11:30, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://pdbe.org/2abh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2abh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 15:52, 25 December 2014

2014-12-25T15:52:23Z

←Older revision		Revision as of 15:52, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI]] Part of the ABC transporter complex PstSACB involved in phosphate import.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Choudhary, A.]]	+	[[Category: Choudhary, A]]
-	[[Category: Ledvina, P S.]]	+	[[Category: Ledvina, P S]]
-	[[Category: Quiocho, F A.]]	+	[[Category: Quiocho, F A]]
-	[[Category: Yao, N.]]	+	[[Category: Yao, N]]
	[[Category: Phosphotransferase]]		[[Category: Phosphotransferase]]
	[[Category: Transport]]		[[Category: Transport]]

OCA at 01:10, 30 September 2014

2014-09-30T01:10:03Z

←Older revision		Revision as of 01:10, 30 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2abh\| PDB=2abh \| SCENE= }}~~	+	==PHOSPHATE-BINDING PROTEIN (RE-REFINED)==
-	===PHOSPHATE-BINDING PROTEIN (RE-REFINED)===	+	<StructureSection load='2abh' size='340' side='right' caption='[[2abh]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_8652549}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ABH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2abh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2abh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2abh RCSB], [http://www.ebi.ac.uk/pdbsum/2abh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ab/2abh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Electrostatic interactions are among the key forces determining the structure and function of proteins. These are exemplified in the liganded form of the receptor, a phosphate binding protein from Escherichia coli. The phosphate, completely dehydrated and buried in the receptor, is bound by 12 hydrogen bonds as well as a salt link with Arg 135. We have modulated the ionic attraction while preserving the hydrogen bonds by mutating Asp 137, also salt linked to Arg 135, to Asn, Gly or Thr. High-resolution crystallographic analysis revealed that Gly and Thr (but not Asn) mutant proteins have incorporated a more electronegative Cl- in place of the Asp carboxylate. That no dramatic effect on phosphate affinity was produced by these ionic perturbations indicates a major role for hydrogen bonds and other local dipoles in the binding and charge stabilization of ionic ligands.

-	~~==About this Structure==~~	+	Modulation of a salt link does not affect binding of phosphate to its specific active transport receptor.,Yao N, Ledvina PS, Choudhary A, Quiocho FA Biochemistry. 1996 Feb 20;35(7):2079-85. PMID:8652549<ref>PMID:8652549</ref>
-	~~[[2abh]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http~~:~~//oca.weizmann.ac.il/oca~~-~~bin/send-pdb?obs=1&id=1abh 1abh]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2ABH OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Phosphate-binding protein\|Phosphate-binding protein]]		*[[Phosphate-binding protein\|Phosphate-binding protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008652549<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Choudhary, A.]]		[[Category: Choudhary, A.]]

OCA at 09:59, 11 March 2013

2013-03-11T09:59:23Z

←Older revision		Revision as of 09:59, 11 March 2013
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-	[[Image:2abh.png\|left\|200px]]
-
	{{STRUCTURE_2abh\| PDB=2abh \| SCENE= }}		{{STRUCTURE_2abh\| PDB=2abh \| SCENE= }}
-
	===PHOSPHATE-BINDING PROTEIN (RE-REFINED)===		===PHOSPHATE-BINDING PROTEIN (RE-REFINED)===
-
	{{ABSTRACT_PUBMED_8652549}}		{{ABSTRACT_PUBMED_8652549}}

OCA: Protected "2abh" [edit=sysop:move=sysop]

2013-01-06T10:25:42Z

Protected "2abh" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:25, 6 January 2013

OCA at 10:25, 6 January 2013

2013-01-06T10:25:39Z

←Older revision		Revision as of 10:25, 6 January 2013
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-	{{Seed}}
	[[Image:2abh.png\|left\|200px]]		[[Image:2abh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2abh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2abh\| PDB=2abh \| SCENE= }}		{{STRUCTURE_2abh\| PDB=2abh \| SCENE= }}

	===PHOSPHATE-BINDING PROTEIN (RE-REFINED)===		===PHOSPHATE-BINDING PROTEIN (RE-REFINED)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8652549}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8652549 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_8652549}}		{{ABSTRACT_PUBMED_8652549}}

	==About this Structure==		==About this Structure==
-	~~2ABH~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA].	+	[[2abh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abh 1abh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABH OCA].
		+
		+	==See Also==
		+	*[[Phosphate-binding protein\|Phosphate-binding protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8652549~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008652549</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Choudhary, A.]]		[[Category: Choudhary, A.]]
Line 31:		Line 22:
	[[Category: Phosphotransferase]]		[[Category: Phosphotransferase]]
	[[Category: Transport]]		[[Category: Transport]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:59:00 2009''