2aor - Revision history

OCA at 07:27, 23 August 2023

2023-08-23T07:27:26Z

←Older revision		Revision as of 07:27, 23 August 2023
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	<StructureSection load='2aor' size='340' side='right'caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2aor' size='340' side='right'caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium~~ influenzae~~" lehmann and neumann 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AOR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[2aoq\|2aoq]]~~</~~div~~>~~</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [https://pdbe.org/2aor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [https://www.ebi.ac.uk/pdbsum/2aor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [https://pdbe.org/2aor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [https://www.ebi.ac.uk/pdbsum/2aor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).	+	[https://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 2aor" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2aor" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA mismatch repair protein 3D structures\|DNA mismatch repair protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ influenzae ~~lehmann and neumann 1896~~]]	+	[[Category: Haemophilus influenzae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang, J]]	+	[[Category: Chang J]]
-	[[Category: Ghirlando, R]]	+	[[Category: Ghirlando R]]
-	[[Category: Joseph, N]]	+	[[Category: Joseph N]]
-	[[Category: Lee~~, J Y~~]]	+	[[Category: Lee JY]]
-	[[Category: Rao~~, D N~~]]	+	[[Category: Rao DN]]
-	[[Category: Yang, W]]	+	[[Category: Yang W]]
-	~~[[Category: Gatc recognition]]~~	+
-	~~[[Category: Hydrolase-dna complex~~]]	+

OCA at 08:35, 27 January 2021

2021-01-27T08:35:16Z

←Older revision		Revision as of 08:35, 27 January 2021
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	==Crystal structure of MutH-hemimethylated DNA complex==		==Crystal structure of MutH-hemimethylated DNA complex==
-	<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2aor' size='340' side='right'caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AOR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2aoq\|2aoq]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [~~http~~://pdbe.org/2aor PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2aor PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [https://pdbe.org/2aor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [https://www.ebi.ac.uk/pdbsum/2aor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).	+	[[https://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bacterium influenzae lehmann and neumann 1896]]		[[Category: Bacterium influenzae lehmann and neumann 1896]]
		+	[[Category: Large Structures]]
	[[Category: Chang, J]]		[[Category: Chang, J]]
	[[Category: Ghirlando, R]]		[[Category: Ghirlando, R]]

OCA at 06:54, 9 May 2018

2018-05-09T06:54:05Z

←Older revision		Revision as of 06:54, 9 May 2018
Line 1:		Line 1:
		+
	==Crystal structure of MutH-hemimethylated DNA complex==		==Crystal structure of MutH-hemimethylated DNA complex==
	<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://pdbe.org/2aor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://pdbe.org/2aor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2aor ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/2aor_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/2aor_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aor ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:25, 11 September 2015

2015-09-11T14:25:17Z

←Older revision		Revision as of 14:25, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Haemophilus_influenzae Haemophilus~~ influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AOR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 ~~Haemophilus~~ influenzae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://pdbe.org/2aor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2aor" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Haemophilus~~ influenzae]]	+	[[Category: Bacterium influenzae lehmann and neumann 1896]]
	[[Category: Chang, J]]		[[Category: Chang, J]]
	[[Category: Ghirlando, R]]		[[Category: Ghirlando, R]]

OCA at 17:41, 25 December 2014

2014-12-25T17:41:07Z

←Older revision		Revision as of 17:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AOR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MUTH_HAEIN MUTH_HAEIN]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
-	[[Category: Chang, J.]]	+	[[Category: Chang, J]]
-	[[Category: Ghirlando, R.]]	+	[[Category: Ghirlando, R]]
-	[[Category: Joseph, N.]]	+	[[Category: Joseph, N]]
-	[[Category: Lee, J Y.]]	+	[[Category: Lee, J Y]]
-	[[Category: Rao, D N.]]	+	[[Category: Rao, D N]]
-	[[Category: Yang, W.]]	+	[[Category: Yang, W]]
	[[Category: Gatc recognition]]		[[Category: Gatc recognition]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]

OCA at 00:50, 30 September 2014

2014-09-30T00:50:50Z

←Older revision		Revision as of 00:50, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2aor.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of MutH-hemimethylated DNA complex==
		+	<StructureSection load='2aor' size='340' side='right' caption='[[2aor]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AOR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2aoq\|2aoq]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mutH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aor OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aor RCSB], [http://www.ebi.ac.uk/pdbsum/2aor PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/2aor_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MutH initiates mismatch repair by nicking the transiently unmethylated daughter strand 5' to a GATC sequence. Here, we report crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates. Both structures contain two Ca2+ ions jointly coordinated by a conserved aspartate and the scissile phosphate, as observed in the restriction endonucleases BamHI and BglI. In the hemimethylated complexes, the active site is more compact and DNA cleavage is more efficient. The Lys residue in the conserved DEK motif coordinates the nucleophilic water in conjunction with the phosphate 3' to the scissile bond; the same Lys is also hydrogen bonded with a carbonyl oxygen in the DNA binding module. We propose that this Lys, which is conserved in many restriction endonucleases and is replaced by Glu or Gln in BamHI and BglII, is a sensor for DNA binding and the linchpin that couples base recognition and DNA cleavage.

-	~~{{STRUCTURE_2aor\| PDB=2aor \| SCENE= }}~~	+	MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage.,Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W Mol Cell. 2005 Oct 7;20(1):155-66. PMID:16209953<ref>PMID:16209953</ref>

-	~~===Crystal structure~~ of ~~MutH-hemimethylated DNA complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16209953}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2aor]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Haemophilus_influenzae Haemophilus influenzae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016209953<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
	[[Category: Chang, J.]]		[[Category: Chang, J.]]

OCA: Protected "2aor" [edit=sysop:move=sysop]

2013-01-06T10:10:28Z

Protected "2aor" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:10, 6 January 2013

OCA at 10:10, 6 January 2013

2013-01-06T10:10:24Z

←Older revision		Revision as of 10:10, 6 January 2013
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-	{{Seed}}
	[[Image:2aor.png\|left\|200px]]		[[Image:2aor.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2aor", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2aor\| PDB=2aor \| SCENE= }}		{{STRUCTURE_2aor\| PDB=2aor \| SCENE= }}

	===Crystal structure of MutH-hemimethylated DNA complex===		===Crystal structure of MutH-hemimethylated DNA complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16209953}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16209953 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16209953}}		{{ABSTRACT_PUBMED_16209953}}

	==About this Structure==		==About this Structure==
-	~~2AOR~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].	+	[[2aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16209953~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016209953</ref><references group="xtra"/>
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
	[[Category: Chang, J.]]		[[Category: Chang, J.]]
Line 32:		Line 20:
	[[Category: Yang, W.]]		[[Category: Yang, W.]]
	[[Category: Gatc recognition]]		[[Category: Gatc recognition]]
-		+	[[Category: Hydrolase-dna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:30:38 2009''~~	+

OCA at 22:30, 17 February 2009

2009-02-17T22:30:40Z

←Older revision		Revision as of 22:30, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2AOR is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].	+	2AOR is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOR OCA].

	==Reference==		==Reference==
-	~~MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage., Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W, Mol Cell. 2005 Oct 7;20(1):155-66.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16209953 16209953]~~	+	<ref group="xtra">PMID:16209953</ref><references group="xtra"/>
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
-	[[Category: Single protein]]
	[[Category: Chang, J.]]		[[Category: Chang, J.]]
	[[Category: Ghirlando, R.]]		[[Category: Ghirlando, R.]]
Line 34:		Line 33:
	[[Category: Gatc recognition]]		[[Category: Gatc recognition]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 07~~:53:~~34 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:30:38 2009''

OCA at 04:53, 29 July 2008

2008-07-29T04:53:36Z

←Older revision		Revision as of 04:53, 29 July 2008
Line 1:		Line 1:
-	[[Image:2aor.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2aor.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2aor\| PDB=2aor \| SCENE= }}		{{STRUCTURE_2aor\| PDB=2aor \| SCENE= }}

-	~~'''~~Crystal structure of MutH-hemimethylated DNA complex~~'''~~	+	===Crystal structure of MutH-hemimethylated DNA complex===


-	~~==Overview==~~	+	<!--
-	~~MutH initiates mismatch repair by nicking~~ the ~~transiently unmethylated daughter strand 5'~~ to a GATC sequence. Here, we report crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates. Both structures contain two Ca2+ ions jointly coordinated by a conserved aspartate and the ~~scissile phosphate,~~ as ~~observed in the restriction endonucleases BamHI and BglI~~. ~~In the hemimethylated complexes, the active site is more compact and DNA cleavage is more efficient~~. The Lys residue in the conserved DEK motif coordinates the nucleophilic water in conjunction with the phosphate 3' to the scissile bond; the same Lys is also hydrogen bonded with a carbonyl oxygen in the DNA binding module. We propose that this Lys, ~~which~~ is ~~conserved in many restriction endonucleases and is replaced by Glu or Gln in BamHI and BglII, is a sensor for DNA binding and~~ the ~~linchpin that couples base recognition and DNA cleavage~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16209953}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16209953 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16209953}}

	==About this Structure==		==About this Structure==
Line 29:		Line 33:
	[[Category: Yang, W.]]		[[Category: Yang, W.]]
	[[Category: Gatc recognition]]		[[Category: Gatc recognition]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 19~~:17:54 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:53:34 2008''