2aum - Revision history

OCA at 07:29, 23 August 2023

2023-08-23T07:29:52Z

←Older revision		Revision as of 07:29, 23 August 2023
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	<StructureSection load='2aum' size='340' side='right'caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2aum' size='340' side='right'caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1zrs~~\|~~1zrs~~]]~~</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4~~.17.13 3.4.17.13] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [https://pdbe.org/2aum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [https://www.ebi.ac.uk/pdbsum/2aum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aum ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [https://pdbe.org/2aum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [https://www.ebi.ac.uk/pdbsum/2aum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aum ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LDC_PSEAE LDC_PSEAE]] Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.	+	[https://www.uniprot.org/uniprot/LDC_PSEAE LDC_PSEAE] Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Muramoyltetrapeptide carboxypeptidase~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Bochtler, M]]	+	[[Category: Bochtler M]]
-	[[Category: Korza~~, H J]]~~	+	[[Category: Korza HJ]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ld-carboxypeptidase]]~~	+
-	~~[[Category: Nucleophilic elbow]]~~	+
-	~~[[Category: Peptidoglycan hydrolase]]~~	+
-	~~[[Category: Serine peptidase]]~~	+
-	~~[[Category: Serine-histidine-glutamate triad~~]]	+

OCA at 17:12, 20 October 2021

2021-10-20T17:12:03Z

←Older revision		Revision as of 17:12, 20 October 2021
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	<StructureSection load='2aum' size='340' side='right'caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2aum' size='340' side='right'caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2AUM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zrs\|1zrs]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [~~http~~://pdbe.org/2aum PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2aum PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2aum ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [https://pdbe.org/2aum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [https://www.ebi.ac.uk/pdbsum/2aum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aum ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LDC_PSEAE LDC_PSEAE]] Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.	+	[[https://www.uniprot.org/uniprot/LDC_PSEAE LDC_PSEAE]] Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:02, 13 February 2020

2020-02-13T05:02:29Z

←Older revision		Revision as of 05:02, 13 February 2020
Line 1:		Line 1:

	==Active site Ser115Ala mutant of LD-carboxypeptidase==		==Active site Ser115Ala mutant of LD-carboxypeptidase==
-	<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='2aum' size='340' side='right'caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
	[[Category: Bochtler, M]]		[[Category: Bochtler, M]]

OCA at 06:51, 9 May 2018

2018-05-09T06:51:35Z

←Older revision		Revision as of 06:51, 9 May 2018
Line 1:		Line 1:
		+
	==Active site Ser115Ala mutant of LD-carboxypeptidase==		==Active site Ser115Ala mutant of LD-carboxypeptidase==
	<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://pdbe.org/2aum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://pdbe.org/2aum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2aum ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/2aum_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/2aum_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aum ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:05, 11 September 2015

2015-09-11T15:05:05Z

←Older revision		Revision as of 15:05, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://pdbe.org/2aum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2aum" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Bochtler, M]]		[[Category: Bochtler, M]]
	[[Category: Korza, H J]]		[[Category: Korza, H J]]

OCA at 18:08, 25 December 2014

2014-12-25T18:08:24Z

←Older revision		Revision as of 18:08, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LDC_PSEAE LDC_PSEAE]] Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Bochtler, M.]]	+	[[Category: Bochtler, M]]
-	[[Category: Korza, H J.]]	+	[[Category: Korza, H J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ld-carboxypeptidase]]		[[Category: Ld-carboxypeptidase]]

OCA at 01:38, 30 September 2014

2014-09-30T01:38:32Z

←Older revision		Revision as of 01:38, 30 September 2014
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-	[[~~Image:~~2aum.~~png~~\|~~left~~\|200px]]	+	==Active site Ser115Ala mutant of LD-carboxypeptidase==
		+	<StructureSection load='2aum' size='340' side='right' caption='[[2aum]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AUM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrs\|1zrs]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Muramoyltetrapeptide_carboxypeptidase Muramoyltetrapeptide carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.13 3.4.17.13] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2aum RCSB], [http://www.ebi.ac.uk/pdbsum/2aum PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/2aum_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	LD-Carboxypeptidases (EC 3.4.17.13) are named for their ability to cleave amide bonds between l- and d-amino acids, which occur naturally in bacterial peptidoglycan. They are specific for the link between meso-diaminopimelic acid and d-alanine and therefore degrade GlcNAc-MurNAc tetrapeptides to the corresponding tripeptides. As only the tripeptides can be reused as peptidoglycan building blocks, ld-carboxypeptidases are thought to play a role in peptidoglycan recycling. Despite the pharmaceutical interest in peptidoglycan biosynthesis, the fold and catalytic type of ld-carboxypeptidases are unknown. Here, we show that a previously uncharacterized open reading frame in Pseudomonas aeruginosa has ld-carboxypeptidase activity and present the crystal structure of this enzyme. The structure shows that the enzyme consists of an N-terminal beta-sheet and a C-terminal beta-barrel domain. At the interface of the two domains, Ser(115) adopts a highly strained conformation in the context of a strand-turn-helix motif that is similar to the "nucleophilic elbow" in alphabeta-hydrolases. Ser(115) is hydrogen-bonded to a histidine residue, which is oriented by a glutamate residue. All three residues, which occur in the order Ser-Glu-His in the amino acid sequence, are strictly conserved in naturally occurring ld-carboxypeptidases and cannot be mutated to alanines without loss of activity. We conclude that ld-carboxypeptidases are serine peptidases with Ser-His-Glu catalytic triads.

-	~~{{STRUCTURE_2aum\| PDB=2aum \| SCENE= }}~~	+	Pseudomonas aeruginosa LD-carboxypeptidase, a serine peptidase with a Ser-His-Glu triad and a nucleophilic elbow.,Korza HJ, Bochtler M J Biol Chem. 2005 Dec 9;280(49):40802-12. Epub 2005 Sep 14. PMID:16162494<ref>PMID:16162494</ref>

-	~~===Active site Ser115Ala mutant~~ of ~~LD-carboxypeptidase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16162494}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2aum]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016162494<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]

OCA: Protected "2aum" [edit=sysop:move=sysop]

2013-01-06T10:53:32Z

Protected "2aum" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 6 January 2013

OCA at 10:53, 6 January 2013

2013-01-06T10:53:29Z

←Older revision		Revision as of 10:53, 6 January 2013
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-	{{Seed}}
	[[Image:2aum.png\|left\|200px]]		[[Image:2aum.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2aum\| PDB=2aum \| SCENE= }}		{{STRUCTURE_2aum\| PDB=2aum \| SCENE= }}

	===Active site Ser115Ala mutant of LD-carboxypeptidase===		===Active site Ser115Ala mutant of LD-carboxypeptidase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16162494}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16162494 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16162494}}		{{ABSTRACT_PUBMED_16162494}}

	==About this Structure==		==About this Structure==
-	~~2AUM~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA].	+	[[2aum]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16162494~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016162494</ref><references group="xtra"/>
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Bochtler, M.]]		[[Category: Bochtler, M.]]
	[[Category: Korza, H J.]]		[[Category: Korza, H J.]]
		+	[[Category: Hydrolase]]
	[[Category: Ld-carboxypeptidase]]		[[Category: Ld-carboxypeptidase]]
	[[Category: Nucleophilic elbow]]		[[Category: Nucleophilic elbow]]
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	[[Category: Serine peptidase]]		[[Category: Serine peptidase]]
	[[Category: Serine-histidine-glutamate triad]]		[[Category: Serine-histidine-glutamate triad]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:14:24 2009''

OCA at 23:14, 17 February 2009

2009-02-17T23:14:32Z

←Older revision		Revision as of 23:14, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2AUM is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA].	+	2AUM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUM OCA].

	==Reference==		==Reference==
-	~~Pseudomonas aeruginosa LD-carboxypeptidase, a serine peptidase with a Ser-His-Glu triad and a nucleophilic elbow., Korza HJ, Bochtler M, J Biol Chem. 2005 Dec 9;280(49):40802-12. Epub 2005 Sep 14.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16162494 16162494]~~	+	<ref group="xtra">PMID:16162494</ref><references group="xtra"/>
	[[Category: Muramoyltetrapeptide carboxypeptidase]]		[[Category: Muramoyltetrapeptide carboxypeptidase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Single protein]]
	[[Category: Bochtler, M.]]		[[Category: Bochtler, M.]]
	[[Category: Korza, H J.]]		[[Category: Korza, H J.]]
Line 35:		Line 34:
	[[Category: Serine-histidine-glutamate triad]]		[[Category: Serine-histidine-glutamate triad]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:33:~~37 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:14:24 2009''