2b5k - Revision history

OCA at 16:33, 13 December 2023

2023-12-13T16:33:02Z

←Older revision		Revision as of 16:33, 13 December 2023
Line 1:		Line 1:

	==PV5 NMR solution structure in DPC micelles==		==PV5 NMR solution structure in DPC micelles==
-	<StructureSection load='2b5k' size='340' side='right'caption='[[2b5k~~]], [[NMR_Ensembles_of_Models \| 17 NMR models~~]]' scene=''>	+	<StructureSection load='2b5k' size='340' side='right'caption='[[2b5k]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limulus_polyphemus Limulus polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5K FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NH2:AMINO+GROUP'>NH2</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1rkk\|1rkk]], [[1x7k\|1x7k]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [https://pdbe.org/2b5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [https://www.ebi.ac.uk/pdbsum/2b5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [https://pdbe.org/2b5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [https://www.ebi.ac.uk/pdbsum/2b5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO]] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.	+	[https://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hancock, R E.W~~]]	+	[[Category: Limulus polyphemus]]
-	[[Category: ~~Powers, J P.S~~]]	+	[[Category: Hancock REW]]
-	[[Category: ~~Ramamoorthy, A~~]]	+	[[Category: Powers JPS]]
-	[[Category: ~~Tan,~~ A]]	+	[[Category: Ramamoorthy A]]
-	[[Category: ~~Antimicrobial peptide]]~~	+	[[Category: Tan A]]
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: Beta hairpin]]~~	+
-	~~[[Category: Disulfide bridge]]~~	+
-	~~[[Category: Polyphemusin variant]]~~	+
-	~~[[Category: Pv5~~]]	+

OCA at 13:05, 24 November 2021

2021-11-24T13:05:37Z

←Older revision		Revision as of 13:05, 24 November 2021
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	<StructureSection load='2b5k' size='340' side='right'caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>		<StructureSection load='2b5k' size='340' side='right'caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2B5K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5K FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rkk\|1rkk]], [[1x7k\|1x7k]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [~~http~~://pdbe.org/2b5k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2b5k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [https://pdbe.org/2b5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [https://www.ebi.ac.uk/pdbsum/2b5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO]] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.	+	[[https://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO]] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:09, 19 February 2020

2020-02-19T08:09:31Z

←Older revision		Revision as of 08:09, 19 February 2020
Line 1:		Line 1:

	==PV5 NMR solution structure in DPC micelles==		==PV5 NMR solution structure in DPC micelles==
-	<StructureSection load='2b5k' size='340' side='right' caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>	+	<StructureSection load='2b5k' size='340' side='right'caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B5K FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B5K FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hancock, R E.W]]		[[Category: Hancock, R E.W]]
	[[Category: Powers, J P.S]]		[[Category: Powers, J P.S]]

OCA at 06:36, 16 May 2018

2018-05-16T06:36:20Z

←Older revision		Revision as of 06:36, 16 May 2018
Line 1:		Line 1:
		+
	==PV5 NMR solution structure in DPC micelles==		==PV5 NMR solution structure in DPC micelles==
	<StructureSection load='2b5k' size='340' side='right' caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>		<StructureSection load='2b5k' size='340' side='right' caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://pdbe.org/2b5k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://pdbe.org/2b5k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:54, 10 September 2015

2015-09-10T21:54:35Z

←Older revision		Revision as of 21:54, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://pdbe.org/2b5k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2b5k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:35, 25 December 2014

2014-12-25T06:35:38Z

←Older revision		Revision as of 06:35, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PPM1_LIMPO PPM1_LIMPO]] Significantly inhibits the growth of Gram-negative and Gram-positive bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hancock, R E.W.]]	+	[[Category: Hancock, R E.W]]
-	[[Category: Powers, J P.S.]]	+	[[Category: Powers, J P.S]]
-	[[Category: Ramamoorthy, A.]]	+	[[Category: Ramamoorthy, A]]
-	[[Category: Tan, A.]]	+	[[Category: Tan, A]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]
	[[Category: Antimicrobial protein]]		[[Category: Antimicrobial protein]]

OCA at 08:16, 8 October 2014

2014-10-08T08:16:53Z

←Older revision		Revision as of 08:16, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2b5k.~~png~~\|~~left~~\|~~200px~~]]	+	==PV5 NMR solution structure in DPC micelles==
		+	<StructureSection load='2b5k' size='340' side='right' caption='[[2b5k]], [[NMR_Ensembles_of_Models \| 17 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B5K FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rkk\|1rkk]], [[1x7k\|1x7k]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b5k RCSB], [http://www.ebi.ac.uk/pdbsum/2b5k PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The horseshoe crab cationic antimicrobial peptide polyphemusin I is highly active in vitro but not protective in mouse models of bacterial and LPS challenge, while a synthetic polyphemusin variant, PV5, was previously shown to be protective in vivo. In this study, we investigated the interaction of these peptides with lipid membranes in an effort to propose a mechanism of interaction. The solution structure of PV5 was determined by proton NMR in the absence and presence of dodecylphosphocholine (DPC) micelles. Like polyphemusin I, PV5 is a beta-hairpin but appeared less amphipathic in solution. Upon association with DPC micelles, PV5 underwent side chain rearrangements which resulted in an increased amphipathic conformation. Using fluorescence spectroscopy, both peptides were found to have limited affinity for neutral vesicles composed of phosphatidylcholine (PC). Incorporation of 25 mol % cholesterol or phosphatidylethanolamine into PC vesicles produced little change in the partitioning of either peptide. Incorporation of 25 mol % phosphatidylglycerol (PG) into PC vesicles, a simple prokaryotic model, resulted in a large increase in the affinity for both peptides, but the partition coefficient for PV5 was almost twice that of polyphemusin I. Differential scanning calorimetry studies supported the partitioning data and demonstrated that neither peptide interacted readily with neutral PC vesicles. Both peptides showed affinity for negatively charged membranes incorporating PG. The affinity of PV5 was much greater as the pretransition peak was absent at low peptide to lipid ratios (1:400) and the reduction in enthalpy of the main transition was greater than that produced by polyphemusin I. Both peptides decreased the lamellar to inverted hexagonal phase transition temperature of PE indicating the induction of negative curvature strain. These results, combined with previous findings that polyphemusin I promotes lipid flip-flop but does not induce significant vesicle leakage, ruled out the torroidal pore and carpet mechanisms of antimicrobial action for these polyphemusins.

-	~~{{STRUCTURE_2b5k\| PDB=2b5k \| SCENE= }}~~	+	Solution structure and interaction of the antimicrobial polyphemusins with lipid membranes.,Powers JP, Tan A, Ramamoorthy A, Hancock RE Biochemistry. 2005 Nov 29;44(47):15504-13. PMID:16300399<ref>PMID:16300399</ref>

-	~~===PV5 NMR solution structure in DPC micelles===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16300399}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2b5k]] is~~ a ~~1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2B5K OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016300399<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Hancock, R E.W.]]		[[Category: Hancock, R E.W.]]
	[[Category: Powers, J P.S.]]		[[Category: Powers, J P.S.]]

OCA: Protected "2b5k" [edit=sysop:move=sysop]

2013-01-06T11:57:38Z

Protected "2b5k" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:57, 6 January 2013

OCA at 11:57, 6 January 2013

2013-01-06T11:57:36Z

←Older revision		Revision as of 11:57, 6 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2b5k.png\|left\|200px]]		[[Image:2b5k.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2b5k", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2b5k\| PDB=2b5k \| SCENE= }}		{{STRUCTURE_2b5k\| PDB=2b5k \| SCENE= }}

	===PV5 NMR solution structure in DPC micelles===		===PV5 NMR solution structure in DPC micelles===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16300399}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16300399 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16300399}}		{{ABSTRACT_PUBMED_16300399}}

	==About this Structure==		==About this Structure==
-	~~2B5K is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA].	+	[[2b5k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA].

	==Reference==		==Reference==
-	~~Solution structure and interaction of the antimicrobial polyphemusins with lipid membranes., Powers JP, Tan A, Ramamoorthy A, Hancock RE, Biochemistry. 2005 Nov 29;44(47):15504-13.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16300399 16300399]~~	+	<ref group="xtra">PMID:016300399</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Hancock, R E.W.]]		[[Category: Hancock, R E.W.]]
	[[Category: Powers, J P.S.]]		[[Category: Powers, J P.S.]]
Line 30:		Line 17:
	[[Category: Tan, A.]]		[[Category: Tan, A.]]
	[[Category: Antimicrobial peptide]]		[[Category: Antimicrobial peptide]]
		+	[[Category: Antimicrobial protein]]
	[[Category: Beta hairpin]]		[[Category: Beta hairpin]]
	[[Category: Disulfide bridge]]		[[Category: Disulfide bridge]]
	[[Category: Polyphemusin variant]]		[[Category: Polyphemusin variant]]
	[[Category: Pv5]]		[[Category: Pv5]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:07:46 2008''

OCA at 10:07, 28 July 2008

2008-07-28T10:07:49Z

←Older revision		Revision as of 10:07, 28 July 2008
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-	[[Image:2b5k.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2b5k.png\|left\|200px]]

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	{{STRUCTURE_2b5k\| PDB=2b5k \| SCENE= }}		{{STRUCTURE_2b5k\| PDB=2b5k \| SCENE= }}

-	~~'''~~PV5 NMR solution structure in DPC micelles~~'''~~	+	===PV5 NMR solution structure in DPC micelles===


-	~~==Overview==~~	+	<!--
-	The ~~horseshoe crab cationic antimicrobial peptide polyphemusin I is highly active in vitro but not protective in mouse models of bacterial and LPS challenge~~, ~~while a synthetic polyphemusin variant~~, ~~PV5, was previously shown to be protective in vivo. In this study, we investigated~~ the ~~interaction of these peptides with lipid membranes in an effort~~ to ~~propose a mechanism of interaction. The solution structure of PV5 was determined by proton NMR in~~ the ~~absence and presence of dodecylphosphocholine~~ (DPC) micelles. Like polyphemusin I, PV5 is a beta-hairpin but appeared less amphipathic in solution. Upon association with DPC micelles, PV5 underwent side chain rearrangements which resulted in an increased amphipathic conformation. Using fluorescence spectroscopy, both peptides were found to have limited affinity for neutral vesicles composed of phosphatidylcholine (PC). Incorporation of 25 mol % cholesterol or phosphatidylethanolamine into PC vesicles produced little change in the partitioning of either peptide. Incorporation of 25 mol % phosphatidylglycerol (PG) into PC vesicles, a simple prokaryotic model, resulted in a large increase in the affinity for both peptides, but the partition coefficient for PV5 was almost twice that of polyphemusin I. Differential scanning calorimetry studies supported the partitioning data and demonstrated that neither peptide interacted readily with neutral PC vesicles. Both peptides showed affinity for negatively charged membranes incorporating PG. The affinity of PV5 was much greater as ~~the pretransition peak was absent~~ at ~~low peptide to lipid ratios (1~~:~~400) and the reduction in enthalpy of the main transition was greater than that produced by polyphemusin I~~. ~~Both peptides decreased the lamellar to inverted hexagonal phase transition temperature of PE indicating the induction of negative curvature strain~~. ~~These results~~, ~~combined with previous findings that polyphemusin I promotes lipid flip-flop but does not induce significant vesicle leakage, ruled out~~ the ~~torroidal pore and carpet mechanisms of antimicrobial action for these polyphemusins~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16300399}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16300399 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16300399}}

	==About this Structure==		==About this Structure==
-	2B5K is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA].	+	2B5K is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5K OCA].

	==Reference==		==Reference==
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	[[Category: Polyphemusin variant]]		[[Category: Polyphemusin variant]]
	[[Category: Pv5]]		[[Category: Pv5]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 19~~:53:04 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:07:46 2008''