2b88 - Revision history

OCA at 05:33, 15 May 2024

2024-05-15T05:33:27Z

←Older revision		Revision as of 05:33, 15 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B88 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [https://pdbe.org/2b88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [https://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [https://pdbe.org/2b88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [https://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:25, 14 June 2023

2023-06-14T08:25:16Z

←Older revision		Revision as of 08:25, 14 June 2023
Line 1:		Line 1:

	==Structural basis for molecular recognition in an affibody:affibody complex==		==Structural basis for molecular recognition in an affibody:affibody complex==
-	<StructureSection load='2b88' size='340' side='right'caption='[[2b88~~]], [[NMR_Ensembles_of_Models \| 40 NMR models~~]]' scene=''>	+	<StructureSection load='2b88' size='340' side='right'caption='[[2b88]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B88 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2b87\|2b87]], [[2b89\|2b89]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [https://pdbe.org/2b88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [https://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [https://pdbe.org/2b88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [https://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q70AB8_STAAU Q70AB8_STAAU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Dogan, J~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Hard, T~~]]	+	[[Category: Dogan J]]
-	[[Category: ~~Lendel, C~~]]	+	[[Category: Hard T]]
-	[[Category: ~~Affibody]]~~	+	[[Category: Lendel C]]
-	~~[[Category: Induced fit]]~~	+
-	~~[[Category: Molecular recognition]]~~	+
-	~~[[Category: Nmr spectroscopy]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Protein-protein interaction~~]]	+

OCA at 06:45, 1 December 2021

2021-12-01T06:45:21Z

←Older revision		Revision as of 06:45, 1 December 2021
Line 3:		Line 3:
	<StructureSection load='2b88' size='340' side='right'caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2b88' size='340' side='right'caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2B88 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2b87\|2b87]], [[2b89\|2b89]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [~~http~~://pdbe.org/2b88 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [https://pdbe.org/2b88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [https://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:17, 19 February 2020

2020-02-19T08:17:04Z

←Older revision		Revision as of 08:17, 19 February 2020
Line 1:		Line 1:

	==Structural basis for molecular recognition in an affibody:affibody complex==		==Structural basis for molecular recognition in an affibody:affibody complex==
-	<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>	+	<StructureSection load='2b88' size='340' side='right'caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
Line 30:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Dogan, J]]		[[Category: Dogan, J]]
	[[Category: Hard, T]]		[[Category: Hard, T]]

OCA at 06:48, 16 May 2018

2018-05-16T06:48:20Z

←Older revision		Revision as of 06:48, 16 May 2018
Line 1:		Line 1:
		+
	==Structural basis for molecular recognition in an affibody:affibody complex==		==Structural basis for molecular recognition in an affibody:affibody complex==
	<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://pdbe.org/2b88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://pdbe.org/2b88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b88 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/2b88_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/2b88_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:04, 8 February 2016

2016-02-08T12:04:58Z

←Older revision		Revision as of 12:04, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b88 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:24, 10 September 2015

2015-09-10T11:24:13Z

←Older revision		Revision as of 11:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://pdbe.org/2b88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2b88" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Dogan, J]]		[[Category: Dogan, J]]
	[[Category: Hard, T]]		[[Category: Hard, T]]

OCA at 10:36, 8 January 2015

2015-01-08T10:36:30Z

←Older revision		Revision as of 10:36, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Dogan, J.]]	+	[[Category: Dogan, J]]
-	[[Category: Hard, T.]]	+	[[Category: Hard, T]]
-	[[Category: Lendel, C.]]	+	[[Category: Lendel, C]]
	[[Category: Affibody]]		[[Category: Affibody]]
	[[Category: Induced fit]]		[[Category: Induced fit]]

OCA at 01:19, 30 September 2014

2014-09-30T01:19:28Z

←Older revision		Revision as of 01:19, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2b88.~~png~~\|~~left~~\|200px]]	+	==Structural basis for molecular recognition in an affibody:affibody complex==
		+	<StructureSection load='2b88' size='340' side='right' caption='[[2b88]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2b88]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B88 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b87\|2b87]], [[2b89\|2b89]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b88 RCSB], [http://www.ebi.ac.uk/pdbsum/2b88 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/2b88_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Affibody molecules constitute a class of engineered binding proteins based on the 58-residue three-helix bundle Z domain derived from staphylococcal protein A (SPA). Affibody proteins are selected as binders to target proteins by phage display of combinatorial libraries in which typically 13 side-chains on the surface of helices 1 and 2 in the Z domain have been randomized. The Z(Taq):anti-Z(Taq) affibody-affibody complex, consisting of Z(Taq), originally selected as a binder to Taq DNA polymerase, and anti-Z(Taq), selected as binder to Z(Taq), is formed with a dissociation constant K(d) approximately 100 nM. We have determined high-precision solution structures of free Z(Taq) and anti-Z(Taq), and the Z(Taq):anti-Z(Taq) complex under identical experimental conditions (25 degrees C in 50 mM NaCl with 20 mM potassium phosphate buffer at pH 6.4). The complex is formed with helices 1 and 2 of anti-Z(Taq) in perpendicular contact with helices 1 and 2 of Z(Taq). The interaction surface is large ( approximately 1670 A(2)) and unusually non-polar (70 %) compared to other protein-protein complexes. It involves all varied residues on anti-Z(Taq), most corresponding (Taq DNA polymerase binding) side-chains on Z(Taq), and several additional side-chain and backbone contacts. Other notable features include a substantial rearrangement (induced fit) of aromatic side-chains in Z(Taq) upon binding, a close contact between glycine residues in the two subunits that might involve aliphatic glycine Halpha to backbone carbonyl hydrogen bonds, and four hydrogen bonds made by the two guanidinium N(eta)H(2) groups of an arginine side-chain. Comparisons of the present structure with other data for affibody proteins and the Z domain suggest that intrinsic binding properties of the originating SPA surface might be inherited by the affibody binders. A thermodynamic characterization of Z(Taq) and anti-Z(Taq) is presented in an accompanying paper.

-	~~{{STRUCTURE_2b88\| PDB=2b88 \| SCENE= }}~~	+	Structural basis for molecular recognition in an affibody:affibody complex.,Lendel C, Dogan J, Hard T J Mol Biol. 2006 Jun 23;359(5):1293-304. Epub 2006 May 6. PMID:16750222<ref>PMID:16750222</ref>

-	~~===Structural basis for molecular recognition in an affibody:affibody complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16750222}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2b88]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2B88 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016750222<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Dogan, J.]]		[[Category: Dogan, J.]]

OCA: Protected "2b88" [edit=sysop:move=sysop]

2013-01-06T10:34:22Z

Protected "2b88" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:34, 6 January 2013