2bjc - Revision history

OCA at 11:22, 22 May 2024

2024-05-22T11:22:03Z

←Older revision		Revision as of 11:22, 22 May 2024
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	==NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor==		==NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor==
-	<StructureSection load='2bjc' size='340' side='right'caption='[[2bjc~~]], [[NMR_Ensembles_of_Models \| 16 NMR models~~]]' scene=''>	+	<StructureSection load='2bjc' size='340' side='right'caption='[[2bjc]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~='overflow: auto; max-height: 3em;'>[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</div></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [https://pdbe.org/2bjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [https://www.ebi.ac.uk/pdbsum/2bjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [https://pdbe.org/2bjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [https://www.ebi.ac.uk/pdbsum/2bjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI]] Repressor of the lactose operon. Binds allolactose as an inducer.	+	[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boelens, R]]	+	[[Category: Boelens R]]
-	[[Category: Bonvin~~, A M.J J~~]]	+	[[Category: Bonvin AMJJ]]
-	[[Category: Das, D]]	+	[[Category: Das D]]
-	[[Category: Folkers~~, G E~~]]	+	[[Category: Folkers GE]]
-	[[Category: Kaptein, R]]	+	[[Category: Kaptein R]]
-	[[Category: Salinas~~, R K]]~~	+	[[Category: Salinas RK]]
-	~~[[Category: Altered specificity]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Gal operon]]~~	+
-	~~[[Category: Gal repressor]]~~	+
-	~~[[Category: Hth]]~~	+
-	~~[[Category: Lac headpiece]]~~	+
-	~~[[Category: Lac operon]]~~	+
-	~~[[Category: Lac repressor]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Symmetric dimer]]~~	+
-	~~[[Category: Symmetric dna-binding]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transcription regulator]]~~	+
-	~~[[Category: Transcription regulator/dna~~]]	+

OCA at 13:11, 24 November 2021

2021-11-24T13:11:14Z

←Older revision		Revision as of 13:11, 24 November 2021
Line 3:		Line 3:
	<StructureSection load='2bjc' size='340' side='right'caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2bjc' size='340' side='right'caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [~~http~~://pdbe.org/2bjc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bjc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [https://pdbe.org/2bjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [https://www.ebi.ac.uk/pdbsum/2bjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI]] Repressor of the lactose operon. Binds allolactose as an inducer.	+	[[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI]] Repressor of the lactose operon. Binds allolactose as an inducer.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:23, 19 February 2020

2020-02-19T08:23:11Z

←Older revision		Revision as of 08:23, 19 February 2020
Line 1:		Line 1:

	==NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor==		==NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor==
-	<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>	+	<StructureSection load='2bjc' size='340' side='right'caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Boelens, R]]		[[Category: Boelens, R]]
	[[Category: Bonvin, A M.J J]]		[[Category: Bonvin, A M.J J]]

OCA at 06:57, 16 May 2018

2018-05-16T06:57:29Z

←Older revision		Revision as of 06:57, 16 May 2018
Line 1:		Line 1:
-	==NMR ~~STRUCTURE OF A PROTEIN~~-DNA ~~COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR~~==	+
		+	==NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor==
	<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://pdbe.org/2bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://pdbe.org/2bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bjc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:

	==See Also==		==See Also==
-	*[[Lac repressor ~~3D structures~~\|Lac repressor ~~3D structures~~]]	+	*[[Lac repressor\|Lac repressor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:58, 11 September 2015

2015-09-11T14:58:01Z

←Older revision		Revision as of 14:58, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://pdbe.org/2bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2bjc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Boelens, R]]		[[Category: Boelens, R]]
	[[Category: Bonvin, A M.J J]]		[[Category: Bonvin, A M.J J]]

OCA at 18:02, 25 December 2014

2014-12-25T18:02:44Z

←Older revision		Revision as of 18:02, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI]] Repressor of the lactose operon. Binds allolactose as an inducer.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Boelens, R.]]	+	[[Category: Boelens, R]]
-	[[Category: Bonvin, A M.J J.]]	+	[[Category: Bonvin, A M.J J]]
-	[[Category: Das, D.]]	+	[[Category: Das, D]]
-	[[Category: Folkers, G E.]]	+	[[Category: Folkers, G E]]
-	[[Category: Kaptein, R.]]	+	[[Category: Kaptein, R]]
-	[[Category: Salinas, R K.]]	+	[[Category: Salinas, R K]]
	[[Category: Altered specificity]]		[[Category: Altered specificity]]
	[[Category: Dna-binding]]		[[Category: Dna-binding]]

OCA at 05:27, 29 September 2014

2014-09-29T05:27:43Z

←Older revision		Revision as of 05:27, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2bjc.~~png~~\|~~left~~\|200px]]	+	==NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR==
		+	<StructureSection load='2bjc' size='340' side='right' caption='[[2bjc]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cjg\|1cjg]], [[1efa\|1efa]], [[1jwl\|1jwl]], [[1jye\|1jye]], [[1jyf\|1jyf]], [[1l1m\|1l1m]], [[1lbg\|1lbg]], [[1lbh\|1lbh]], [[1lbi\|1lbi]], [[1lcc\|1lcc]], [[1lcd\|1lcd]], [[1lqc\|1lqc]], [[1ltp\|1ltp]], [[1osl\|1osl]], [[1tlf\|1tlf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bjc RCSB], [http://www.ebi.ac.uk/pdbsum/2bjc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex of an altered-specificity mutant lac headpiece in which Tyr17 and Gln18 were exchanged for valine and alanine, respectively, as found in the recognition helix of the gal repressor. As previously described by Lehming et al. (EMBO J. 1987, 6, 3145-3153), this altered-specificity mutant of the lac repressor recognizes a variant lac operator that is similar to the gal operator Oe. The mutant lac headpiece showed the predicted specificity and is also able to mimic the gal repressor by recognizing and bending the natural gal operator Oe. These structural data show that, while most of the anchoring points that help the lac headpiece to assemble on the lac operator were preserved, a different network of protein-DNA interactions connecting Ala17 and Val18 to bases in the DNA major groove drives the specificity towards the altered operator.

-	~~{{STRUCTURE_2bjc\| PDB=2bjc \| SCENE= }}~~	+	Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor.,Kopke Salinas R, Folkers GE, Bonvin AM, Das D, Boelens R, Kaptein R Chembiochem. 2005 Sep;6(9):1628-37. PMID:16094693<ref>PMID:16094693</ref>

-	~~===NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16094693}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2bjc]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].~~	+

	==See Also==		==See Also==
-	*[[Lac repressor\|Lac repressor]]	+	*[[Lac repressor 3D structures\|Lac repressor 3D structures]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016094693<~~/~~ref~~><~~ref group="xtra">PMID:012065400</ref><ref group="xtra">PMID:002826131</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Boelens, R.]]		[[Category: Boelens, R.]]

OCA at 19:45, 20 October 2012

2012-10-20T19:45:10Z

←Older revision		Revision as of 19:45, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2bjc]] is a 4 chain structure ~~of [[Lac repressor]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].	+	[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].

	==See Also==		==See Also==

OCA at 08:21, 27 July 2012

2012-07-27T08:21:57Z

←Older revision		Revision as of 08:21, 27 July 2012
Line 1:		Line 1:
	[[Image:2bjc.png\|left\|200px]]		[[Image:2bjc.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2bjc", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2bjc\| PDB=2bjc \| SCENE= }}		{{STRUCTURE_2bjc\| PDB=2bjc \| SCENE= }}

	===NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR===		===NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16094693}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16094693 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16094693}}		{{ABSTRACT_PUBMED_16094693}}

	==About this Structure==		==About this Structure==
-	[[2bjc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].	+	[[2bjc]] is a 4 chain structure of [[Lac repressor]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA].
		+
		+	==See Also==
		+	*[[Lac repressor\|Lac repressor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16094693~~</ref><ref group="xtra">PMID:~~12065400~~</ref><ref group="xtra">PMID:~~2826131~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016094693</ref><ref group="xtra">PMID:012065400</ref><ref group="xtra">PMID:002826131</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Boelens, R.]]		[[Category: Boelens, R.]]
Line 39:		Line 31:
	[[Category: Lac repressor]]		[[Category: Lac repressor]]
	[[Category: Mutant]]		[[Category: Mutant]]
-	[[Category: Nmr]]
	[[Category: Repressor]]		[[Category: Repressor]]
	[[Category: Symmetric dimer]]		[[Category: Symmetric dimer]]

OCA: Protected "2bjc" [edit=sysop:move=sysop]

2011-03-14T21:17:36Z

Protected "2bjc" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:17, 14 March 2011