2c0a - Revision history

OCA at 09:20, 9 May 2024

2024-05-09T09:20:45Z

←Older revision		Revision as of 09:20, 9 May 2024
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	<StructureSection load='2c0a' size='340' side='right'caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2c0a' size='340' side='right'caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C0A FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [https://pdbe.org/2c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [https://www.ebi.ac.uk/pdbsum/2c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [https://pdbe.org/2c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [https://www.ebi.ac.uk/pdbsum/2c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ALKH_ECOLI ALKH_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fullerton~~, S W.B~~]]	+	[[Category: Fullerton SWB]]
-	[[Category: Hutchins~~, M J~~]]	+	[[Category: Hutchins MJ]]
-	[[Category: Naismith~~, J H~~]]	+	[[Category: Naismith JH]]
-	[[Category: Toone~~, E J]]~~	+	[[Category: Toone EJ]]
-	~~[[Category: Aldolase]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Multifunctional enzyme]]~~	+
-	~~[[Category: Schiff base~~]]	+

OCA at 13:21, 24 November 2021

2021-11-24T13:21:50Z

←Older revision		Revision as of 13:21, 24 November 2021
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	<StructureSection load='2c0a' size='340' side='right'caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2c0a' size='340' side='right'caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2C0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [~~http~~://pdbe.org/2c0a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2c0a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [https://pdbe.org/2c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [https://www.ebi.ac.uk/pdbsum/2c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:16, 19 February 2020

2020-02-19T08:16:26Z

←Older revision		Revision as of 08:16, 19 February 2020
Line 1:		Line 1:

	==Mechanism of the Class I KDPG aldolase==		==Mechanism of the Class I KDPG aldolase==
-	<StructureSection load='2c0a' size='340' side='right' caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='2c0a' size='340' side='right'caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0A FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Aldolase\|Aldolase]]	+	*[[Aldolase 3D structures\|Aldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Fullerton, S W.B]]		[[Category: Fullerton, S W.B]]
	[[Category: Hutchins, M J]]		[[Category: Hutchins, M J]]

OCA at 06:47, 16 May 2018

2018-05-16T06:47:19Z

←Older revision		Revision as of 06:47, 16 May 2018
Line 1:		Line 1:
		+
	==Mechanism of the Class I KDPG aldolase==		==Mechanism of the Class I KDPG aldolase==
	<StructureSection load='2c0a' size='340' side='right' caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2c0a' size='340' side='right' caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://pdbe.org/2c0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://pdbe.org/2c0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c0a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/2c0a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/2c0a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:41, 9 February 2016

2016-02-09T01:41:10Z

←Older revision		Revision as of 01:41, 9 February 2016
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://pdbe.org/2c0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c0a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2c0a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:24, 15 January 2015

2015-01-15T18:24:48Z

←Older revision		Revision as of 18:24, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
-	[[Category: Fullerton, S W.B.]]	+	[[Category: Fullerton, S W.B]]
-	[[Category: Hutchins, M J.]]	+	[[Category: Hutchins, M J]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
-	[[Category: Toone, E J.]]	+	[[Category: Toone, E J]]
	[[Category: Aldolase]]		[[Category: Aldolase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Multifunctional enzyme]]		[[Category: Multifunctional enzyme]]
	[[Category: Schiff base]]		[[Category: Schiff base]]

OCA at 09:05, 3 October 2014

2014-10-03T09:05:45Z

←Older revision		Revision as of 09:05, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2c0a~~\| ~~PDB~~=2c0a \| ~~SCENE~~= }}	+	==Mechanism of the Class I KDPG aldolase==
-	===~~Mechanism~~ of the ~~Class I KDPG aldolase~~===	+	<StructureSection load='2c0a' size='340' side='right' caption='[[2c0a]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_16403639}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C0A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]], [[1wau\|1wau]], [[1wbh\|1wbh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c0a RCSB], [http://www.ebi.ac.uk/pdbsum/2c0a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/2c0a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In vivo, 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase catalyzes the reversible, stereospecific retro-aldol cleavage of KDPG to pyruvate and D-glyceraldehyde-3-phosphate. The enzyme is a lysine-dependent (Class I) aldolase that functions through the intermediacy of a Schiff base. Here, we propose a mechanism for this enzyme based on crystallographic studies of wild-type and mutant aldolases. The three dimensional structure of KDPG aldolase from the thermophile Thermotoga maritima was determined to 1.9A. The structure is the standard alpha/beta barrel observed for all Class I aldolases. At the active site Lys we observe clear density for a pyruvate Schiff base. Density for a sulfate ion bound in a conserved cluster of residues close to the Schiff base is also observed. We have also determined the structure of a mutant of Escherichia coli KDPG aldolase in which the proposed general acid/base catalyst has been removed (E45N). One subunit of the trimer contains density suggesting a trapped pyruvate carbinolamine intermediate. All three subunits contain a phosphate ion bound in a location effectively identical to that of the sulfate ion bound in the T. maritima enzyme. The sulfate and phosphate ions experimentally locate the putative phosphate binding site of the aldolase and, together with the position of the bound pyruvate, facilitate construction of a model for the full-length KDPG substrate complex. The model requires only minimal positional adjustments of the experimentally determined covalent intermediate and bound anion to accommodate full-length substrate. The model identifies the key catalytic residues of the protein and suggests important roles for two observable water molecules. The first water molecule remains bound to the enzyme during the entire catalytic cycle, shuttling protons between the catalytic glutamate and the substrate. The second water molecule arises from dehydration of the carbinolamine and serves as the nucleophilic water during hydrolysis of the enzyme-product Schiff base. The second water molecule may also mediate the base-catalyzed enolization required to form the carbon nucleophile, again bridging to the catalytic glutamate. Many aspects of this mechanism are observed in other Class I aldolases and suggest a mechanistically and, perhaps, evolutionarily related family of aldolases distinct from the N-acetylneuraminate lyase (NAL) family.

-	~~==About this Structure==~~	+	Mechanism of the Class I KDPG aldolase.,Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH Bioorg Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PMID:16403639<ref>PMID:16403639</ref>
-	~~[[2c0a]] is a 3 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2C0A OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:016403639</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Fullerton, S W.B.]]		[[Category: Fullerton, S W.B.]]

OCA at 05:50, 4 December 2013

2013-12-04T05:50:38Z

←Older revision		Revision as of 05:50, 4 December 2013
Line 1:		Line 1:
-	[[Image:2c0a.png\|left\|200px]]
-
	{{STRUCTURE_2c0a\| PDB=2c0a \| SCENE= }}		{{STRUCTURE_2c0a\| PDB=2c0a \| SCENE= }}
-		+	===Mechanism of the Class I KDPG aldolase===
-	===~~MECHANISM OF THE CLASS~~ I KDPG ~~ALDOLASE~~===	+
-		+
	{{ABSTRACT_PUBMED_16403639}}		{{ABSTRACT_PUBMED_16403639}}

	==About this Structure==		==About this Structure==
-	[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA].	+	[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA].

	==See Also==		==See Also==
Line 14:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016403639</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016403639</ref><references group="xtra"/><references/>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Fullerton, S W.B.]]		[[Category: Fullerton, S W.B.]]
	[[Category: Hutchins, M J.]]		[[Category: Hutchins, M J.]]

OCA at 12:12, 20 October 2012

2012-10-20T12:12:17Z

←Older revision		Revision as of 12:12, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2c0a]] is a 3 chain structure ~~of [[Aldolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA].	+	[[2c0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C0A OCA].

	==See Also==		==See Also==

OCA: Protected "2c0a" [edit=sysop:move=sysop]

2012-07-26T07:56:39Z

Protected "2c0a" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:56, 26 July 2012