2cgy - Revision history

OCA at 14:16, 13 December 2023

2023-12-13T14:16:59Z

←Older revision		Revision as of 14:16, 13 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CGY FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CGY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=~~NAG~~:N-ACETYL-D-~~GLUCOSAMINE~~'>~~NAG~~</scene>, <scene name='pdbligand=~~A2G~~:N-ACETYL-2-~~DEOXY-2-AMINO-GALACTOSE~~'>~~A2G~~</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>, <scene name='pdbligand=PRD_900083:Forssman+antigen+fragment'>PRD_900083</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [https://pdbe.org/2cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [https://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [https://pdbe.org/2cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [https://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2F1K8_HELPO Q2F1K8_HELPO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Helix pomatia]]		[[Category: Helix pomatia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Audfray, A]]	+	[[Category: Audfray A]]
-	[[Category: Breton, C]]	+	[[Category: Breton C]]
-	[[Category: Chazalet, V]]	+	[[Category: Chazalet V]]
-	[[Category: Gagnon, J]]	+	[[Category: Gagnon J]]
-	[[Category: Gautier, C]]	+	[[Category: Gautier C]]
-	[[Category: Imberty, A]]	+	[[Category: Imberty A]]
-	[[Category: Lescar, J]]	+	[[Category: Lescar J]]
-	[[Category: Mitchell~~, E P~~]]	+	[[Category: Mitchell EP]]
-	[[Category: Sanchez, J F]]	+	[[Category: Sanchez J-F]]
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Snail]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 09:17, 24 February 2021

2021-02-24T09:17:42Z

←Older revision		Revision as of 09:17, 24 February 2021
Line 1:		Line 1:

-	====	+	==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==
-	<StructureSection load='2cgy' size='340' side='right'caption='[[2cgy]]' scene=''>	+	<StructureSection load='2cgy' size='340' side='right'caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CGY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [https://pdbe.org/2cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [https://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [https://pdbe.org/2cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [https://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 18:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cgy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cgy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Helix pomatia agglutinin (HPA) is a lectin that has been used extensively in histopathology, since its binding to tissue sections from breast and colon cancers is correlated with the worst prognosis for the patients. The lectin recognizes alpha-d-N-acetylgalactosamine (alphaGalNAc) containing epitopes which are only present in cancer cell lines having a high likelihood to undergo metastasis, such as the HT29 cancer colon cell line. Several breast cancer cell lines have also been shown to be labeled, although IGROV1, an ovarian cancer cell line, is not. Inhibition studies, using GalNAc monosaccharides, are reported here, showing that the labeling is dependent upon the presence of carbohydrate epitopes. The crystal structures of the lectin complexed with two GalNAc containing epitopes associated with cancer, the Tn (alphaGalNAc-Ser) and Forssman (alphaGalNAc1-3GalNAc) antigens, show the lectin's specificity for GalNAc is due to a particular network of hydrogen bonds. A histidine residue makes hydrophobic contact with the aglycon, rationalizing the preference for GalNAc bearing an additional sugar or amino acid in the alpha position. These structures provide the molecular basis for the use of HPA in metastasis research.
		+
		+	Structural basis for recognition of breast and colon cancer epitopes Tn antigen and Forssman disaccharide by Helix pomatia lectin.,Lescar J, Sanchez JF, Audfray A, Coll JL, Breton C, Mitchell EP, Imberty A Glycobiology. 2007 Oct;17(10):1077-83. Epub 2007 Jul 25. PMID:17652409<ref>PMID:17652409</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2cgy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Agglutinin 3D structures\|Agglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Helix pomatia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Audfray, A]]
		+	[[Category: Breton, C]]
		+	[[Category: Chazalet, V]]
		+	[[Category: Gagnon, J]]
		+	[[Category: Gautier, C]]
		+	[[Category: Imberty, A]]
		+	[[Category: Lescar, J]]
		+	[[Category: Mitchell, E P]]
		+	[[Category: Sanchez, J F]]
		+	[[Category: Lectin]]
		+	[[Category: Snail]]
		+	[[Category: Sugar binding protein]]

OCA at 07:11, 24 February 2021

2021-02-24T07:11:58Z

←Older revision		Revision as of 07:11, 24 February 2021
Line 1:		Line 1:

-	==~~STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN~~==	+	====
-	<StructureSection load='2cgy' size='340' side='right'caption='[[2cgy]]~~, [[Resolution\|resolution]] 2.80Å~~' scene=''>	+	<StructureSection load='2cgy' size='340' side='right'caption='[[2cgy]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~2CGY~~ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=~~2CGY~~ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [https://pdbe.org/2cgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [https://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [~~http~~://pdbe.org/2cgy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>	+
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 18:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cgy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cgy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Helix pomatia agglutinin (HPA) is a lectin that has been used extensively in histopathology, since its binding to tissue sections from breast and colon cancers is correlated with the worst prognosis for the patients. The lectin recognizes alpha-d-N-acetylgalactosamine (alphaGalNAc) containing epitopes which are only present in cancer cell lines having a high likelihood to undergo metastasis, such as the HT29 cancer colon cell line. Several breast cancer cell lines have also been shown to be labeled, although IGROV1, an ovarian cancer cell line, is not. Inhibition studies, using GalNAc monosaccharides, are reported here, showing that the labeling is dependent upon the presence of carbohydrate epitopes. The crystal structures of the lectin complexed with two GalNAc containing epitopes associated with cancer, the Tn (alphaGalNAc-Ser) and Forssman (alphaGalNAc1-3GalNAc) antigens, show the lectin's specificity for GalNAc is due to a particular network of hydrogen bonds. A histidine residue makes hydrophobic contact with the aglycon, rationalizing the preference for GalNAc bearing an additional sugar or amino acid in the alpha position. These structures provide the molecular basis for the use of HPA in metastasis research.
-
-	Structural basis for recognition of breast and colon cancer epitopes Tn antigen and Forssman disaccharide by Helix pomatia lectin.,Lescar J, Sanchez JF, Audfray A, Coll JL, Breton C, Mitchell EP, Imberty A Glycobiology. 2007 Oct;17(10):1077-83. Epub 2007 Jul 25. PMID:17652409<ref>PMID:17652409</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2cgy" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Agglutinin 3D structures\|Agglutinin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Helix pomatia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Audfray, A]]~~	+	[[Category: Z-disk]]
-	~~[[Category: Breton, C]]~~	+
-	~~[[Category: Chazalet, V]]~~	+
-	~~[[Category: Gagnon, J]]~~	+
-	~~[[Category: Gautier, C]]~~	+
-	~~[[Category: Imberty, A]]~~	+
-	~~[[Category: Lescar, J]]~~	+
-	~~[[Category: Mitchell, E P]]~~	+
-	~~[[Category: Sanchez, J F]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Snail]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 11:59, 10 May 2019

2019-05-10T11:59:31Z

←Older revision		Revision as of 11:59, 10 May 2019
Line 1:		Line 1:

	==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==		==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==
-	<StructureSection load='2cgy' size='340' side='right' caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2cgy' size='340' side='right'caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CGY FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CGY FirstGlance]. <br>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Agglutinin\|Agglutinin]]	+	*[[Agglutinin 3D structures\|Agglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Helix pomatia]]		[[Category: Helix pomatia]]
		+	[[Category: Large Structures]]
	[[Category: Audfray, A]]		[[Category: Audfray, A]]
	[[Category: Breton, C]]		[[Category: Breton, C]]

OCA at 08:04, 23 May 2018

2018-05-23T08:04:14Z

←Older revision		Revision as of 08:04, 23 May 2018
Line 1:		Line 1:
		+
	==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==		==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==
	<StructureSection load='2cgy' size='340' side='right' caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2cgy' size='340' side='right' caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://pdbe.org/2cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://pdbe.org/2cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cgy ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cgy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cgy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cgy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 2cgy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2cgy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Agglutinin\|Agglutinin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:29, 11 September 2015

2015-09-11T10:29:23Z

←Older revision		Revision as of 10:29, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://pdbe.org/2cgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2cgy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:13, 15 January 2015

2015-01-15T16:13:00Z

←Older revision		Revision as of 16:13, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CGY FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CGY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Helix pomatia]]		[[Category: Helix pomatia]]
-	[[Category: Audfray, A.]]	+	[[Category: Audfray, A]]
-	[[Category: Breton, C.]]	+	[[Category: Breton, C]]
-	[[Category: Chazalet, V.]]	+	[[Category: Chazalet, V]]
-	[[Category: Gagnon, J.]]	+	[[Category: Gagnon, J]]
-	[[Category: Gautier, C.]]	+	[[Category: Gautier, C]]
-	[[Category: Imberty, A.]]	+	[[Category: Imberty, A]]
-	[[Category: Lescar, J.]]	+	[[Category: Lescar, J]]
-	[[Category: Mitchell, E P.]]	+	[[Category: Mitchell, E P]]
-	[[Category: Sanchez, J F.]]	+	[[Category: Sanchez, J F]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Snail]]		[[Category: Snail]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 08:22, 3 October 2014

2014-10-03T08:22:58Z

←Older revision		Revision as of 08:22, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2cgy\| PDB=2cgy \| SCENE= }}~~	+	==STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN==
-	===STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN===	+	<StructureSection load='2cgy' size='340' side='right' caption='[[2cgy]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_17652409}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CGY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FSM:FORSMANN+ANTIGEN'>FSM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ccv\|2ccv]], [[2ce6\|2ce6]], [[2cgz\|2cgz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cgy RCSB], [http://www.ebi.ac.uk/pdbsum/2cgy PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cgy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Helix pomatia agglutinin (HPA) is a lectin that has been used extensively in histopathology, since its binding to tissue sections from breast and colon cancers is correlated with the worst prognosis for the patients. The lectin recognizes alpha-d-N-acetylgalactosamine (alphaGalNAc) containing epitopes which are only present in cancer cell lines having a high likelihood to undergo metastasis, such as the HT29 cancer colon cell line. Several breast cancer cell lines have also been shown to be labeled, although IGROV1, an ovarian cancer cell line, is not. Inhibition studies, using GalNAc monosaccharides, are reported here, showing that the labeling is dependent upon the presence of carbohydrate epitopes. The crystal structures of the lectin complexed with two GalNAc containing epitopes associated with cancer, the Tn (alphaGalNAc-Ser) and Forssman (alphaGalNAc1-3GalNAc) antigens, show the lectin's specificity for GalNAc is due to a particular network of hydrogen bonds. A histidine residue makes hydrophobic contact with the aglycon, rationalizing the preference for GalNAc bearing an additional sugar or amino acid in the alpha position. These structures provide the molecular basis for the use of HPA in metastasis research.

-	~~==About this Structure==~~	+	Structural basis for recognition of breast and colon cancer epitopes Tn antigen and Forssman disaccharide by Helix pomatia lectin.,Lescar J, Sanchez JF, Audfray A, Coll JL, Breton C, Mitchell EP, Imberty A Glycobiology. 2007 Oct;17(10):1077-83. Epub 2007 Jul 25. PMID:17652409<ref>PMID:17652409</ref>
-	~~[[2cgy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia~~ Helix pomatia]. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2CGY OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:017652409</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Helix pomatia]]		[[Category: Helix pomatia]]
	[[Category: Audfray, A.]]		[[Category: Audfray, A.]]

OCA at 08:25, 23 April 2014

2014-04-23T08:25:11Z

←Older revision		Revision as of 08:25, 23 April 2014
Line 1:		Line 1:
-	[[Image:2cgy.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2cgy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2cgy\| PDB=2cgy \| SCENE= }}		{{STRUCTURE_2cgy\| PDB=2cgy \| SCENE= }}
-
	===STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN===		===STRUCTURE OF HELIX POMATIA AGGLUTININ WITH FORSMANN ANTIGEN===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17652409}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17652409 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17652409}}		{{ABSTRACT_PUBMED_17652409}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:017652409</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017652409</ref><references group="xtra"/><references/>
	[[Category: Helix pomatia]]		[[Category: Helix pomatia]]
	[[Category: Audfray, A.]]		[[Category: Audfray, A.]]

OCA: Protected "2cgy" [edit=sysop:move=sysop]

2011-09-28T04:56:33Z

Protected "2cgy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:56, 28 September 2011